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Protein

DNA polymerase clamp

Gene

45

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Replisome processivity clamp subunit that increases the processivity of DNA synthesis. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.1 Publication

GO - Biological processi

  • bidirectional double-stranded viral DNA replication Source: UniProtKB
  • DNA replication Source: UniProtKB-KW

Keywordsi

Biological processDNA replication, Viral DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase clamp1 Publication
Alternative name(s):
DNA polymerase accessory protein 45
DNA polymerase sliding clamp1 Publication
Gp45
Gene namesi
Name:45
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492291 – 228DNA polymerase clampAdd BLAST228

Interactioni

Subunit structurei

Homotrimer (PubMed:26735934). Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the helicase and the helicase assembly factor (PubMed:16800624, PubMed:26102578). Interacts with the DNA polymerase; this interaction constitutes the polymerase holoenzyme (PubMed:8475061).4 Publications

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04525
SMRiP04525
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04525

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK18943
OrthoDBiVOG090000N8

Family and domain databases

InterProiView protein in InterPro
IPR004190 DNA_pol_proc_fac
IPR015200 Gp45_slide_clamp_C
PfamiView protein in Pfam
PF02916 DNA_PPF, 1 hit
PF09116 gp45-slide_C, 1 hit

Sequencei

Sequence statusi: Complete.

P04525-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLSKDTTAL LKNFATINSG IMLKSGQFIM TRAVNGTTYA EANISDVIDF
60 70 80 90 100
DVAIYDLNGF LGILSLVNDD AEISQSEDGN IKIADARSTI FWPAADPSTV
110 120 130 140 150
VAPNKPIPFP VASAVTEIKA EDLQQLLRVS RGLQIDTIAI TVKEGKIVIN
160 170 180 190 200
GFNKVEDSAL TRVKYSLTLG DYDGENTFNF IINMANMKMQ PGNYKLLLWA
210 220
KGKQGAAKFE GEHANYVVAL EADSTHDF
Length:228
Mass (Da):24,858
Last modified:December 19, 2001 - v3
Checksum:i765DF4AAB76D005B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69Missing in CAA24777 (PubMed:6284751).Curated1
Sequence conflicti93P → R in CAA24777 (PubMed:6284751).Curated1
Sequence conflicti93P → R in CAA25340 (PubMed:6284751).Curated1
Sequence conflicti93P → R in AAC05393 (PubMed:6284751).Curated1
Sequence conflicti100V → D in CAA24777 (PubMed:6284751).Curated1
Sequence conflicti129V → L in CAA24777 (PubMed:6284751).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01535 Genomic DNA Translation: CAA24777.1
M10160 Genomic DNA Translation: AAC05393.1
X00769 Genomic DNA Translation: CAA25340.1
AF158101 Genomic DNA Translation: AAD42470.1
M15080 Genomic DNA Translation: AAA32519.1
PIRiA04301 IDBP44
RefSeqiNP_049666.1, NC_000866.4

Genome annotation databases

GeneIDi1258821
KEGGivg:1258821

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01535 Genomic DNA Translation: CAA24777.1
M10160 Genomic DNA Translation: AAC05393.1
X00769 Genomic DNA Translation: CAA25340.1
AF158101 Genomic DNA Translation: AAD42470.1
M15080 Genomic DNA Translation: AAA32519.1
PIRiA04301 IDBP44
RefSeqiNP_049666.1, NC_000866.4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CZDX-ray2.45A/B/C1-228[»]
3U5ZX-ray3.50F/G/H/P/Q/R1-228[»]
3U60X-ray3.34F/G/H1-228[»]
3U61X-ray3.20F/G/H1-228[»]
6DRTX-ray2.12A/B/C1-228[»]
ProteinModelPortaliP04525
SMRiP04525
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258821
KEGGivg:1258821

Phylogenomic databases

KOiK18943
OrthoDBiVOG090000N8

Miscellaneous databases

EvolutionaryTraceiP04525

Family and domain databases

InterProiView protein in InterPro
IPR004190 DNA_pol_proc_fac
IPR015200 Gp45_slide_clamp_C
PfamiView protein in Pfam
PF02916 DNA_PPF, 1 hit
PF09116 gp45-slide_C, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPA5_BPT4
AccessioniPrimary (citable) accession number: P04525
Secondary accession number(s): Q9T0Q0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: December 19, 2001
Last modified: November 7, 2018
This is version 95 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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