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Protein

Tegument protein VP16

Gene

UL48

Organism
Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection (By similarity).By similarity1 Publication
May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei442Critical role in activationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processHost-virus interaction, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Tegument protein VP16
Alternative name(s):
Alpha trans-inducing protein
Alpha-TIF
ICP25
Vmw65
Gene namesi
ORF Names:UL48
OrganismiHuman herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10304 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001157991 – 490Tegument protein VP16Add BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineBy similarity1
Modified residuei353PhosphoserineBy similarity1
Modified residuei411PhosphoserineBy similarity1
Modified residuei452PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP04486

Interactioni

Subunit structurei

Interacts with VP22. Interacts with gH (via C-terminus). Interacts with the virion host shutoff protein (vhs). Interacts with VP11/12. Associates with the VP16-induced complex; binding to host HCFC1 activates VP16 for association with the octamer motif-binding host protein POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes (By similarity).By similarity

Structurei

Secondary structure

1490
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiP04486
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04486

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni411 – 490Transcriptional activationBy similarityAdd BLAST80

Domaini

The transcriptional activation region seems to target many proteins of the RNA polymerase II transcription machinery.By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.1290.10, 1 hit
InterProiView protein in InterPro
IPR003174 Alpha_TIF
IPR036538 Alpha_TIF_sf
IPR021051 HSV_VP16_C
PfamiView protein in Pfam
PF02232 Alpha_TIF, 1 hit
PF12149 HSV_VP16_C, 1 hit
SMARTiView protein in SMART
SM00814 Alpha_TIF, 1 hit
SUPFAMiSSF56548 SSF56548, 1 hit

Sequencei

Sequence statusi: Complete.

P04486-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDLLVDELFA DMDADGASPP PPRPAGGPKN TPAAPPLYAT GRLSQAQLMP
60 70 80 90 100
SPPMPVPPAA LFNRLLDDLG FSAGPALCTM LDTWNEDLFS ALPTNADLYR
110 120 130 140 150
ECKFLSTLPS DVVEWGDAYV PERAQIDIRA HGDVAFPTLP ATRDGLGLYY
160 170 180 190 200
EALSRFFHAE LRAREESYRT VLANFCSALY RYLRASVRQL HRQAHMRGRD
210 220 230 240 250
RDLGEMLRAT IADRYYRETA RLARVLFLHL YLFLTREILW AAYAEQMMRP
260 270 280 290 300
DLFDCLCCDL ESWRQLAGLF QPFMFVNGAL TVRGVPIEAR RLRELNHIRE
310 320 330 340 350
HLNLPLVRSA ATEEPGAPLT TPPTLHGNQA RASGYFMVLI RAKLDSYSSF
360 370 380 390 400
TTSPSEAVMR EHAYSRARTK NNYGSTIEGL LDLPDDDAPE EAGLAAPRLS
410 420 430 440 450
FLPAGHTRRL STAPPTDVSL GDELHLDGED VAMAHADALD DFDLDMLGDG
460 470 480 490
DSPGPGFTPH DSAPYGALDM ADFEFEQMFT DALGIDEYGG
Length:490
Mass (Da):54,316
Last modified:September 22, 2009 - v2
Checksum:i014372DE8FE6C417
GO

Sequence cautioni

The sequence AAA45766 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K03350 Genomic DNA Translation: AAA45766.1 Different initiation.
PIRiA03727 IXBE1F

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K03350 Genomic DNA Translation: AAA45766.1 Different initiation.
PIRiA03727 IXBE1F

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2K2UNMR-B456-490[»]
SMRiP04486
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP04486

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP04486

Family and domain databases

Gene3Di1.10.1290.10, 1 hit
InterProiView protein in InterPro
IPR003174 Alpha_TIF
IPR036538 Alpha_TIF_sf
IPR021051 HSV_VP16_C
PfamiView protein in Pfam
PF02232 Alpha_TIF, 1 hit
PF12149 HSV_VP16_C, 1 hit
SMARTiView protein in SMART
SM00814 Alpha_TIF, 1 hit
SUPFAMiSSF56548 SSF56548, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVP16_HHV1F
AccessioniPrimary (citable) accession number: P04486
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: September 22, 2009
Last modified: November 7, 2018
This is version 79 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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