Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proto-oncogene Wnt-1

Gene

Wnt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (By similarity). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (PubMed:15454084, PubMed:16116452). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (PubMed:2202907, PubMed:16116452). Has a role in osteoblast function, bone development and bone homeostasis (By similarity).By similarity3 Publications

Miscellaneous

Many mouse mammary tumors induced by mouse mammary tumor virus (MMTV) contain a provirus integrated into a host cell region which has been named INT-1 (now Wnt1).1 Publication

Caution

A palmitoylation site was proposed at Cys-93, but it was later shown that this cysteine is engaged in a disulfide bond.By similarity

GO - Molecular functioni

  • cytokine activity Source: BHF-UCL
  • frizzled binding Source: GO_Central
  • protein domain specific binding Source: UniProtKB
  • receptor ligand activity Source: ParkinsonsUK-UCL
  • signaling receptor binding Source: MGI
  • transcription regulatory region DNA binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-3238698 WNT ligand biogenesis and trafficking
R-MMU-4086400 PCP/CE pathway
R-MMU-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane

Names & Taxonomyi

Protein namesi
Recommended name:
Proto-oncogene Wnt-1
Alternative name(s):
Proto-oncogene Int-12 Publications
Gene namesi
Name:Wnt1
Synonyms:Int-13 Publications, Wnt-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:98953 Wnt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Important perinatal lethality, due to defects in brain development. After 14.5 dpc, embryos display dramatic malformations of the mesencephalon and metencephalon, and especially the cerebellum. They show mild midbrain hydrocephaly by 17.5 dpc. Only one out of ten live-born pups survives more than 30 days; these mice respond normally to light, sound, smell and touch, but display severe ataxia.1 Publication

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000004140628 – 370Proto-oncogene Wnt-1Add BLAST343

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi29N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi93 ↔ 104By similarity
Disulfide bondi143 ↔ 151By similarity
Disulfide bondi153 ↔ 170By similarity
Disulfide bondi218 ↔ 232By similarity
Disulfide bondi220 ↔ 227By similarity
Lipidationi224O-palmitoleoyl serine; by PORCNBy similarity1
Disulfide bondi315 ↔ 330By similarity
Glycosylationi316N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi345 ↔ 360By similarity
Glycosylationi346N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi347 ↔ 357By similarity
Disulfide bondi352 ↔ 353By similarity
Glycosylationi359N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Palmitoleoylation is required for efficient binding to frizzled receptors. Palmitoleoylation is necessary for proper trafficking to cell surface (By similarity). Depalmitoleoylated by NOTUM, leading to inhibit Wnt signaling pathway (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiP04426
PRIDEiP04426

PTM databases

iPTMnetiP04426
PhosphoSitePlusiP04426
SwissPalmiP04426

Expressioni

Tissue specificityi

Testis and mid-gestational embryos. In the testis, detected only in postmeiotic germ cells undergoing differentiation from round spermatids into mature spermatozoa. In the embryos, expression is restricted to the developing CNS in regions of the neural tube other than the telencephalon. Expressed in osteoblast; expression levels increase with advancing osteoblast differentiation. Expressed in the brain, femur, spleen, and hematopoietic bone marrow.3 Publications

Developmental stagei

Accumulates throughout the neural plate at the anterior head folds of the 9 day embryo but only at its lateral tips in more posterior regions. Following neural tube closure, expression is restricted to specific regions of the dorsal wall of the brain ventricles and spinal cord, the ventral wall of the midbrain and the diencephalon, and the lateral walls of the neuroepithelium at the midbrain-hindbrain junction.1 Publication

Gene expression databases

BgeeiENSMUSG00000022997 Expressed in 76 organ(s), highest expression level in brain
CleanExiMM_WNT1
ExpressionAtlasiP04426 baseline and differential
GenevisibleiP04426 MM

Interactioni

Subunit structurei

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity. The complex with AFM may represent the physiological form in body fluids (By similarity). Interacts with PORCN (PubMed:10866835). Interacts with RSPO1, RSPO2 and RSPO3 (PubMed:16543246). Interacts with WLS (PubMed:19841259).By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204567, 9 interactors
CORUMiP04426
DIPiDIP-39896N
IntActiP04426, 4 interactors
STRINGi10090.ENSMUSP00000023734

Structurei

3D structure databases

ProteinModelPortaliP04426
SMRiP04426
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
GeneTreeiENSGT00760000118943
HOGENOMiHOG000039528
HOVERGENiHBG001595
InParanoidiP04426
KOiK03209
OMAiWNCPTAS
OrthoDBiEOG091G0OFF
PhylomeDBiP04426
TreeFamiTF105310

Family and domain databases

InterProiView protein in InterPro
IPR005817 Wnt
IPR009139 Wnt1
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 2 hits
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01841 WNT1PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04426-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLWALLPSW VSTTLLLALT ALPAALAANS SGRWWGIVNI ASSTNLLTDS
60 70 80 90 100
KSLQLVLEPS LQLLSRKQRR LIRQNPGILH SVSGGLQSAV RECKWQFRNR
110 120 130 140 150
RWNCPTAPGP HLFGKIVNRG CRETAFIFAI TSAGVTHSVA RSCSEGSIES
160 170 180 190 200
CTCDYRRRGP GGPDWHWGGC SDNIDFGRLF GREFVDSGEK GRDLRFLMNL
210 220 230 240 250
HNNEAGRTTV FSEMRQECKC HGMSGSCTVR TCWMRLPTLR AVGDVLRDRF
260 270 280 290 300
DGASRVLYGN RGSNRASRAE LLRLEPEDPA HKPPSPHDLV YFEKSPNFCT
310 320 330 340 350
YSGRLGTAGT AGRACNSSSP ALDGCELLCC GRGHRTRTQR VTERCNCTFH
360 370
WCCHVSCRNC THTRVLHECL
Length:370
Mass (Da):41,086
Last modified:August 13, 1987 - v1
Checksum:i02EEB23109231A40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02593 Genomic DNA Translation: AAA39321.1
M11943 mRNA Translation: AAA39322.1
BC005449 mRNA Translation: AAH05449.1
CCDSiCCDS27807.1
PIRiA23447 TVMST1
RefSeqiNP_067254.1, NM_021279.4
UniGeneiMm.1123

Genome annotation databases

EnsembliENSMUST00000023734; ENSMUSP00000023734; ENSMUSG00000022997
GeneIDi22408
KEGGimmu:22408
UCSCiuc007xnx.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02593 Genomic DNA Translation: AAA39321.1
M11943 mRNA Translation: AAA39322.1
BC005449 mRNA Translation: AAH05449.1
CCDSiCCDS27807.1
PIRiA23447 TVMST1
RefSeqiNP_067254.1, NM_021279.4
UniGeneiMm.1123

3D structure databases

ProteinModelPortaliP04426
SMRiP04426
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204567, 9 interactors
CORUMiP04426
DIPiDIP-39896N
IntActiP04426, 4 interactors
STRINGi10090.ENSMUSP00000023734

PTM databases

iPTMnetiP04426
PhosphoSitePlusiP04426
SwissPalmiP04426

Proteomic databases

PaxDbiP04426
PRIDEiP04426

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023734; ENSMUSP00000023734; ENSMUSG00000022997
GeneIDi22408
KEGGimmu:22408
UCSCiuc007xnx.1 mouse

Organism-specific databases

CTDi7471
MGIiMGI:98953 Wnt1

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
GeneTreeiENSGT00760000118943
HOGENOMiHOG000039528
HOVERGENiHBG001595
InParanoidiP04426
KOiK03209
OMAiWNCPTAS
OrthoDBiEOG091G0OFF
PhylomeDBiP04426
TreeFamiTF105310

Enzyme and pathway databases

ReactomeiR-MMU-3238698 WNT ligand biogenesis and trafficking
R-MMU-4086400 PCP/CE pathway
R-MMU-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane

Miscellaneous databases

PROiPR:P04426
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022997 Expressed in 76 organ(s), highest expression level in brain
CleanExiMM_WNT1
ExpressionAtlasiP04426 baseline and differential
GenevisibleiP04426 MM

Family and domain databases

InterProiView protein in InterPro
IPR005817 Wnt
IPR009139 Wnt1
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 2 hits
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01841 WNT1PROTEIN
PR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWNT1_MOUSE
AccessioniPrimary (citable) accession number: P04426
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 7, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again