UniProtKB - P04425 (GSHB_ECOLI)
Protein
Glutathione synthetase
Gene
gshB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- EC:6.3.2.3
Cofactori
Activity regulationi
Inhibited by 7,8-dihydrofolate, methotrexate and trimethoprim.
: glutathione biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Glutamate--cysteine ligase (gshA), Glutamate--cysteine ligase (gshA)
- Glutathione synthetase (gshB), Glutathione synthetase (gshB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 281 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 283 | Magnesium or manganeseBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 151 – 207 | ATPBy similarityAdd BLAST | 57 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- glutathione synthase activity Source: EcoCyc
- ligase activity, forming carbon-nitrogen bonds Source: GO_Central
- magnesium ion binding Source: EcoCyc
GO - Biological processi
- glutathione biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Ligase |
Biological process | Glutathione biosynthesis |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:GLUTATHIONE-SYN-MONOMER MetaCyc:GLUTATHIONE-SYN-MONOMER |
BRENDAi | 6.3.2.3, 2026 |
UniPathwayi | UPA00142;UER00210 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione synthetase (EC:6.3.2.3)Alternative name(s): GSH synthetase Short name: GSH-S Short name: GSHase Glutathione synthase |
Gene namesi | Name:gshB Synonyms:gsh-II Ordered Locus Names:b2947, JW2914 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 122 | C → A: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 195 | C → A: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 222 | C → A: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 227 | P → V: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 233 | R → A or K: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 240 | G → V: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 241 | R → A or K: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 289 | C → A: No loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197465 | 1 – 316 | Glutathione synthetaseAdd BLAST | 316 |
Proteomic databases
jPOSTi | P04425 |
PaxDbi | P04425 |
PRIDEi | P04425 |
Interactioni
Subunit structurei
Homotetramer.
Protein-protein interaction databases
BioGRIDi | 4259406, 20 interactors 851765, 1 interactor |
DIPi | DIP-9840N |
IntActi | P04425, 5 interactors |
STRINGi | 511145.b2947 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P04425 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04425 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 125 – 310 | ATP-graspAdd BLAST | 186 |
Sequence similaritiesi
Belongs to the prokaryotic GSH synthase family.Curated
Phylogenomic databases
eggNOGi | COG0189, Bacteria |
HOGENOMi | CLU_068239_0_0_6 |
InParanoidi | P04425 |
PhylomeDBi | P04425 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
HAMAPi | MF_00162, GSH_S, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR006284, Glut_synth_pro IPR004218, GSHS_ATP-bd IPR004215, GSHS_N IPR016185, PreATP-grasp_dom_sf |
PANTHERi | PTHR21621:SF4, PTHR21621:SF4, 1 hit |
Pfami | View protein in Pfam PF02955, GSH-S_ATP, 1 hit PF02951, GSH-S_N, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01380, glut_syn, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
i Sequence
Sequence statusi: Complete.
P04425-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIKLGIVMDP IANINIKKDS SFAMLLEAQR RGYELHYMEM GDLYLINGEA
60 70 80 90 100
RAHTRTLNVK QNYEEWFSFV GEQDLPLADL DVILMRKDPP FDTEFIYATY
110 120 130 140 150
ILERAEEKGT LIVNKPQSLR DCNEKLFTAW FSDLTPETLV TRNKAQLKAF
160 170 180 190 200
WEKHSDIILK PLDGMGGASI FRVKEGDPNL GVIAETLTEH GTRYCMAQNY
210 220 230 240 250
LPAIKDGDKR VLVVDGEPVP YCLARIPQGG ETRGNLAAGG RGEPRPLTES
260 270 280 290 300
DWKIARQIGP TLKEKGLIFV GLDIIGDRLT EINVTSPTCI REIEAEFPVS
310
ITGMLMDAIE ARLQQQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01666 Genomic DNA Translation: CAA25826.1 U28377 Genomic DNA Translation: AAA69114.1 U00096 Genomic DNA Translation: AAC75984.1 AP009048 Genomic DNA Translation: BAE77010.1 |
PIRi | A01194, SYECGS |
RefSeqi | NP_417422.1, NC_000913.3 WP_000593273.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC75984; AAC75984; b2947 BAE77010; BAE77010; BAE77010 |
GeneIDi | 58463729 947445 |
KEGGi | ecj:JW2914 eco:b2947 |
PATRICi | fig|1411691.4.peg.3786 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01666 Genomic DNA Translation: CAA25826.1 U28377 Genomic DNA Translation: AAA69114.1 U00096 Genomic DNA Translation: AAC75984.1 AP009048 Genomic DNA Translation: BAE77010.1 |
PIRi | A01194, SYECGS |
RefSeqi | NP_417422.1, NC_000913.3 WP_000593273.1, NZ_STEB01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GLV | X-ray | 2.70 | A | 1-316 | [»] | |
1GSA | X-ray | 2.00 | A | 1-316 | [»] | |
1GSH | X-ray | 2.00 | A | 1-316 | [»] | |
2GLT | X-ray | 2.20 | A | 1-316 | [»] | |
SMRi | P04425 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259406, 20 interactors 851765, 1 interactor |
DIPi | DIP-9840N |
IntActi | P04425, 5 interactors |
STRINGi | 511145.b2947 |
Proteomic databases
jPOSTi | P04425 |
PaxDbi | P04425 |
PRIDEi | P04425 |
Genome annotation databases
EnsemblBacteriai | AAC75984; AAC75984; b2947 BAE77010; BAE77010; BAE77010 |
GeneIDi | 58463729 947445 |
KEGGi | ecj:JW2914 eco:b2947 |
PATRICi | fig|1411691.4.peg.3786 |
Organism-specific databases
EchoBASEi | EB0414 |
Phylogenomic databases
eggNOGi | COG0189, Bacteria |
HOGENOMi | CLU_068239_0_0_6 |
InParanoidi | P04425 |
PhylomeDBi | P04425 |
Enzyme and pathway databases
UniPathwayi | UPA00142;UER00210 |
BioCyci | EcoCyc:GLUTATHIONE-SYN-MONOMER MetaCyc:GLUTATHIONE-SYN-MONOMER |
BRENDAi | 6.3.2.3, 2026 |
Miscellaneous databases
EvolutionaryTracei | P04425 |
PROi | PR:P04425 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
HAMAPi | MF_00162, GSH_S, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR006284, Glut_synth_pro IPR004218, GSHS_ATP-bd IPR004215, GSHS_N IPR016185, PreATP-grasp_dom_sf |
PANTHERi | PTHR21621:SF4, PTHR21621:SF4, 1 hit |
Pfami | View protein in Pfam PF02955, GSH-S_ATP, 1 hit PF02951, GSH-S_N, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01380, glut_syn, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GSHB_ECOLI | |
Accessioni | P04425Primary (citable) accession number: P04425 Secondary accession number(s): Q2M9P6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | April 7, 2021 | |
This is version 186 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families