UniProtKB - P04413 (US03_HHV11)
Protein
Serine/threonine-protein kinase US3
Gene
US3
Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Functioni
Multifunctional serine/threonine kinase that plays a role in several processes including egress of virus particles from the nucleus, modulation of the actin cytoskeleton and inhibition of apoptosis. Phosphorylates UL31 and UL34, two critical regulators of capsid budding from nucleus to endoplasmic reticulum, thereby facilitating virion egress. Modulates and redistributes host components of the nuclear envelope, including LMNA, emerin/EMD and the nuclear matrix protein MATR3. Phosphorylates envelope glycoprotein B (gB), probably to direct it to the cell surface. Promotes virus intracellular spread by restructuring host cell cytoskeleton. Blocks host apoptosis to extend cell survival and allow efficient viral replication. Promotes viral gene expression by phosphorylating host HDAC2 to reduce viral genome silencing.8 Publications
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 220 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 305 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 197 – 205 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- modulation by virus of host apoptotic process Source: UniProtKB-KW
- modulation by virus of host chromatin organization Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Host-virus interaction, Modulation of host cell apoptosis by virus, Modulation of host chromatin by virus |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:US3 |
Organismi | Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) |
Taxonomic identifieri | 10299 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Peploviricota › Herviviricetes › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Host cytoplasm By similarity
- Host nucleus By similarity
GO - Cellular componenti
- host cell cytoplasm Source: UniProtKB-SubCell
- host cell nucleus Source: UniProtKB-SubCell
- viral tegument Source: CACAO
Keywords - Cellular componenti
Host cytoplasm, Host nucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 147 | S → A: Complete loss of autophosphorylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086180 | 1 – 481 | Serine/threonine-protein kinase US3Add BLAST | 481 |
Post-translational modificationi
Phosphorylated by UL13; this phosphorylation regulates subsequent phosphorylation of UL31 and UL34 by US3. Autophosphorylated.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | P04413 |
PTM databases
iPTMneti | P04413 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 971434, 32 interactors |
IntActi | P04413, 29 interactors |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 191 – 478 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 288 |
Sequence similaritiesi
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P04413-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MACRKFCRVY GGQGRRKEEA VPPETKPSRV FPHGPFYTPA EDACLDSPPP
60 70 80 90 100
ETPKPSHTTP PSEAERLCHL QEILAQMYGN QDYPIEDDPS ADAADDVDED
110 120 130 140 150
APDDVAYPEE YAEELFLPGD ATGPLIGAND HIPPPCGASP PGIRRRSRDE
160 170 180 190 200
IGATGFTAEE LDAMDREAAR AISRGGKPPS TMAKLVTGMG FTIHGALTPG
210 220 230 240 250
SEGCVFDSSH PDYPQRVIVK AGWYTSTSHE ARLLRRLDHP AILPLLDLHV
260 270 280 290 300
VSGVTCLVLP KYQADLYTYL SRRLNPLGRP QIAAVSRQLL SAVDYIHRQG
310 320 330 340 350
IIHRDIKTEN IFINTPEDIC LGDFGAACFV QGSRSSPFPY GIAGTIDTNA
360 370 380 390 400
PEVLAGDPYT TTVDIWSAGL VIFETAVHNA SLFSAPRGPK RGPCDSQITR
410 420 430 440 450
IIRQAQVHVD EFSPHPESRL TSRYRSRAAG NNRPPYTRPA WTRYYKMDID
460 470 480
VEYLVCKALT FDGALRPSAA ELLCLPLFQQ K
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L00036 Genomic DNA Translation: AAA96685.1 X14112 Genomic DNA Translation: CAA32280.1 X02138 Genomic DNA Translation: CAA26057.1 |
PIRi | A00656, TVBE17 |
RefSeqi | YP_009137138.1, NC_001806.2 |
Genome annotation databases
GeneIDi | 2703401 |
KEGGi | vg:2703401 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L00036 Genomic DNA Translation: AAA96685.1 X14112 Genomic DNA Translation: CAA32280.1 X02138 Genomic DNA Translation: CAA26057.1 |
PIRi | A00656, TVBE17 |
RefSeqi | YP_009137138.1, NC_001806.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 971434, 32 interactors |
IntActi | P04413, 29 interactors |
PTM databases
iPTMneti | P04413 |
Proteomic databases
PRIDEi | P04413 |
Genome annotation databases
GeneIDi | 2703401 |
KEGGi | vg:2703401 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | US03_HHV11 | |
Accessioni | P04413Primary (citable) accession number: P04413 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | February 10, 2021 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families