UniProtKB - P04395 (3MG2_ECOLI)
Protein
DNA-3-methyladenine glycosylase 2
Gene
alkA
Organism
Escherichia coli (strain K12)
Status
Functioni
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2-methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions.
Catalytic activityi
- Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine. EC:3.2.2.21
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 218 | Determinant for substrate specificity and/or activity | 1 | |
Active sitei | 238 | Proton acceptor | 1 |
GO - Molecular functioni
- alkylated DNA binding Source: GO_Central
- alkylbase DNA N-glycosylase activity Source: EcoliWiki
- DNA-3-methyladenine glycosylase activity Source: GO_Central
- DNA-3-methylguanine glycosylase activity Source: UniProtKB-EC
- DNA-7-methyladenine glycosylase activity Source: UniProtKB-EC
- DNA-7-methylguanine glycosylase activity Source: GO_Central
GO - Biological processi
- base-excision repair Source: EcoliWiki
- base-excision repair, AP site formation Source: GO_Central
- cellular response to DNA damage stimulus Source: EcoliWiki
- DNA dealkylation involved in DNA repair Source: EcoliWiki
- DNA repair Source: EcoliWiki
Keywordsi
Molecular function | Hydrolase |
Biological process | DNA damage, DNA repair |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11222-MONOMER MetaCyc:EG11222-MONOMER |
BRENDAi | 3.2.2.21, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-3-methyladenine glycosylase 2 (EC:3.2.2.21)Alternative name(s): 3-methyladenine-DNA glycosylase II, inducible Short name: TAG II DNA-3-methyladenine glycosidase II DNA-3-methyladenine glycosylase II |
Gene namesi | Name:alkA Synonyms:aidA Ordered Locus Names:b2068, JW2053 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: EcoliWiki
- protein-DNA complex Source: GO_Central
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 124 | Q → A: Methylmethane sulfonate-resistant. 1 Publication | 1 | |
Mutagenesisi | 218 | W → A: No catalytic activity, methylmethane sulfonate-sensitive. 1 Publication | 1 | |
Mutagenesisi | 237 | D → N: More than 30% catalytic activity, methylmethane sulfonate-resistant. 1 Publication | 1 | |
Mutagenesisi | 238 | D → N: No catalytic activity, methylmethane sulfonate-sensitive. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194878 | 1 – 282 | DNA-3-methyladenine glycosylase 2Add BLAST | 282 |
Proteomic databases
jPOSTi | P04395 |
PaxDbi | P04395 |
PRIDEi | P04395 |
Expressioni
Inductioni
Up-regulated by methylated Ada in response to the exposure to alkylating agents.
Interactioni
Subunit structurei
Monomer.
Binary interactionsi
P04395
With | #Exp. | IntAct |
---|---|---|
aceF [P06959] | 2 | EBI-544077,EBI-542707 |
Protein-protein interaction databases
BioGRIDi | 4259681, 55 interactors |
DIPi | DIP-9084N |
IntActi | P04395, 19 interactors |
STRINGi | 511145.b2068 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P04395 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04395 |
Family & Domainsi
Sequence similaritiesi
Belongs to the alkylbase DNA glycosidase AlkA family.Curated
Phylogenomic databases
eggNOGi | COG0122, Bacteria |
HOGENOMi | CLU_000445_72_3_6 |
InParanoidi | P04395 |
PhylomeDBi | P04395 |
Family and domain databases
CDDi | cd00056, ENDO3c, 1 hit |
Gene3Di | 1.10.1670.10, 1 hit 3.30.310.20, 1 hit |
InterProi | View protein in InterPro IPR010316, AlkA_N IPR037046, AlkA_N_sf IPR000035, Alkylbase_DNA_glycsylse_CS IPR011257, DNA_glycosylase IPR003265, HhH-GPD_domain IPR023170, HTH_base_excis_C |
Pfami | View protein in Pfam PF06029, AlkA_N, 1 hit PF00730, HhH-GPD, 1 hit |
SMARTi | View protein in SMART SM01009, AlkA_N, 1 hit SM00478, ENDO3c, 1 hit |
SUPFAMi | SSF48150, SSF48150, 1 hit |
PROSITEi | View protein in PROSITE PS00516, ALKYLBASE_DNA_GLYCOS, 1 hit |
i Sequence
Sequence statusi: Complete.
P04395-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYTLNWQPPY DWSWMLGFLA ARAVSSVETV ADSYYARSLA VGEYRGVVTA
60 70 80 90 100
IPDIARHTLH INLSAGLEPV AAECLAKMSR LFDLQCNPQI VNGALGRLGA
110 120 130 140 150
ARPGLRLPGC VDAFEQGVRA ILGQLVSVAM AAKLTARVAQ LYGERLDDFP
160 170 180 190 200
EYICFPTPQR LAAADPQALK ALGMPLKRAE ALIHLANAAL EGTLPMTIPG
210 220 230 240 250
DVEQAMKTLQ TFPGIGRWTA NYFALRGWQA KDVFLPDDYL IKQRFPGMTP
260 270 280
AQIRRYAERW KPWRSYALLH IWYTEGWQPD EA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02498 Genomic DNA Translation: AAA23430.1 U00096 Genomic DNA Translation: AAC75129.1 AP009048 Genomic DNA Translation: BAA15926.1 M13827 Genomic DNA No translation available. |
PIRi | A00904, DGECMA |
RefSeqi | NP_416572.1, NC_000913.3 WP_000288420.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75129; AAC75129; b2068 BAA15926; BAA15926; BAA15926 |
GeneIDi | 947371 |
KEGGi | ecj:JW2053 eco:b2068 |
PATRICi | fig|1411691.4.peg.183 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02498 Genomic DNA Translation: AAA23430.1 U00096 Genomic DNA Translation: AAC75129.1 AP009048 Genomic DNA Translation: BAA15926.1 M13827 Genomic DNA No translation available. |
PIRi | A00904, DGECMA |
RefSeqi | NP_416572.1, NC_000913.3 WP_000288420.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DIZ | X-ray | 2.50 | A/B | 1-282 | [»] | |
1MPG | X-ray | 1.80 | A/B | 1-282 | [»] | |
1PVS | X-ray | 2.40 | A/B | 1-282 | [»] | |
3CVS | X-ray | 2.40 | A/B/C/D | 1-282 | [»] | |
3CVT | X-ray | 2.50 | A/B/C/D | 1-282 | [»] | |
3CW7 | X-ray | 2.30 | A/B/C/D | 1-282 | [»] | |
3CWA | X-ray | 2.40 | A/B/C/D | 1-282 | [»] | |
3CWS | X-ray | 2.30 | A/B/C/D | 1-282 | [»] | |
3CWT | X-ray | 2.30 | A/B/C/D | 1-282 | [»] | |
3CWU | X-ray | 2.80 | A/B/C/D | 1-282 | [»] | |
3D4V | X-ray | 2.90 | A/B/C/D | 1-282 | [»] | |
3OGD | X-ray | 2.80 | A | 2-282 | [»] | |
3OH6 | X-ray | 2.89 | A | 2-282 | [»] | |
3OH9 | X-ray | 2.80 | A | 2-282 | [»] | |
SMRi | P04395 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259681, 55 interactors |
DIPi | DIP-9084N |
IntActi | P04395, 19 interactors |
STRINGi | 511145.b2068 |
Proteomic databases
jPOSTi | P04395 |
PaxDbi | P04395 |
PRIDEi | P04395 |
Genome annotation databases
EnsemblBacteriai | AAC75129; AAC75129; b2068 BAA15926; BAA15926; BAA15926 |
GeneIDi | 947371 |
KEGGi | ecj:JW2053 eco:b2068 |
PATRICi | fig|1411691.4.peg.183 |
Organism-specific databases
EchoBASEi | EB1204 |
Phylogenomic databases
eggNOGi | COG0122, Bacteria |
HOGENOMi | CLU_000445_72_3_6 |
InParanoidi | P04395 |
PhylomeDBi | P04395 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11222-MONOMER MetaCyc:EG11222-MONOMER |
BRENDAi | 3.2.2.21, 2026 |
Miscellaneous databases
EvolutionaryTracei | P04395 |
PROi | PR:P04395 |
Family and domain databases
CDDi | cd00056, ENDO3c, 1 hit |
Gene3Di | 1.10.1670.10, 1 hit 3.30.310.20, 1 hit |
InterProi | View protein in InterPro IPR010316, AlkA_N IPR037046, AlkA_N_sf IPR000035, Alkylbase_DNA_glycsylse_CS IPR011257, DNA_glycosylase IPR003265, HhH-GPD_domain IPR023170, HTH_base_excis_C |
Pfami | View protein in Pfam PF06029, AlkA_N, 1 hit PF00730, HhH-GPD, 1 hit |
SMARTi | View protein in SMART SM01009, AlkA_N, 1 hit SM00478, ENDO3c, 1 hit |
SUPFAMi | SSF48150, SSF48150, 1 hit |
PROSITEi | View protein in PROSITE PS00516, ALKYLBASE_DNA_GLYCOS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 3MG2_ECOLI | |
Accessioni | P04395Primary (citable) accession number: P04395 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 1987 |
Last sequence update: | March 20, 1987 | |
Last modified: | December 2, 2020 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families