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Protein

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Gene

NDUFV2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[2Fe-2S] clusterCuratedNote: Binds 1 [2Fe-2S] cluster.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi135Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi140Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi176Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi180Iron-sulfur (2Fe-2S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-BTA-611105 Respiratory electron transport
R-BTA-6799198 Complex I biogenesis

Protein family/group databases

TCDBi3.D.1.6.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
NADH dehydrogenase subunit II
NADH-ubiquinone oxidoreductase 24 kDa subunit
Gene namesi
Name:NDUFV2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 24

Organism-specific databases

VGNCiVGNC:31975 NDUFV2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32Mitochondrion1 PublicationAdd BLAST32
ChainiPRO_000002000233 – 249NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrialAdd BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61N6-acetyllysineBy similarity1
Modified residuei193Phosphotyrosine; by SRCBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP04394
PeptideAtlasiP04394
PRIDEiP04394

Expressioni

Gene expression databases

BgeeiENSBTAG00000004871

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.2 Publications

Protein-protein interaction databases

CORUMiP04394
DIPiDIP-38821N
IntActiP04394, 3 interactors
STRINGi9913.ENSBTAP00000052906

Structurei

3D structure databases

ProteinModelPortaliP04394
SMRiP04394
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 24 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3196 Eukaryota
COG1905 LUCA
GeneTreeiENSGT00390000017580
HOGENOMiHOG000257748
HOVERGENiHBG029601
InParanoidiP04394
KOiK03943
OMAiLTAKDMQ
OrthoDBiEOG091G0GRD
TreeFamiTF300004

Family and domain databases

CDDicd03064 TRX_Fd_NuoE, 1 hit
InterProiView protein in InterPro
IPR002023 NuoE-like
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR10371 PTHR10371, 1 hit
PIRSFiPIRSF000216 NADH_DH_24kDa, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
TIGRFAMsiTIGR01958 nuoE_fam, 1 hit
PROSITEiView protein in PROSITE
PS01099 COMPLEX1_24K, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLSAALRAR AAGLAAHWGK HIRNLHKTAV QNGAGGALFV HRDTPENNPE
60 70 80 90 100
TPFDFTPENY KRIEAIVKNY PEGHKAAAVL PVLDLAQRQN GWLPISAMNK
110 120 130 140 150
VAEILQVPPM RVYEVATFYT MYNRKPVGKY HIQVCTTTPC MLRNSDSILE
160 170 180 190 200
AIQKKLGIKV GETTPDKLFT LIEVECLGAC VNAPMVQIND NYYEDLTPKD
210 220 230 240
IEEIIDELKA GKIPKPGPRS GRFSCEPAGG LTSLTEPPKG PGFGVQAGL
Length:249
Mass (Da):27,308
Last modified:February 1, 1991 - v3
Checksum:i8F7E8259F08EBF65
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89 – 97QNGWLPISA → SSGTSYPDVLKBZZZPPGGA AIILW AA sequence (PubMed:6861757).Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14724 mRNA Translation: CAA32848.1
BC102401 mRNA Translation: AAI02402.1
M22539 mRNA Translation: AAA87358.1
PIRiB30113
RefSeqiNP_776990.1, NM_174565.3
UniGeneiBt.88596

Genome annotation databases

EnsembliENSBTAT00000006405; ENSBTAP00000052906; ENSBTAG00000004871
GeneIDi282290
KEGGibta:282290

Similar proteinsi

Entry informationi

Entry nameiNDUV2_BOVIN
AccessioniPrimary (citable) accession number: P04394
Secondary accession number(s): Q3T0G9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: February 1, 1991
Last modified: March 28, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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