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UniProtKB - P04390 (T2E5_ECOLX)
Protein
Type II restriction enzyme EcoRV
Gene
ecoRVR
Organism
Escherichia coli
Status
Functioni
A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-GATATC-3' and cleaves after T-3.
1 Publication3 PublicationsCatalytic activityi
- Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.2 Publications EC:3.1.21.4
Cofactori
Mg2+Note: Binds 2 magnesium ions per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 45 | Magnesium 2 | 1 | |
Active sitei | 74 | 1 | ||
Metal bindingi | 74 | Magnesium 1 | 1 | |
Metal bindingi | 74 | Magnesium 2 | 1 | |
Active sitei | 90 | 1 | ||
Metal bindingi | 90 | Magnesium 1 | 1 | |
Active sitei | 92 | 1 |
GO - Molecular functioni
- DNA binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
GO - Biological processi
- DNA restriction-modification system Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Endonuclease, Hydrolase, Nuclease |
Biological process | Restriction system |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Type II restriction enzyme EcoRV1 Publication (EC:3.1.21.41 Publication)Short name: R.EcoRV Alternative name(s): Endonuclease EcoRV Type-2 restriction enzyme EcoRV |
Gene namesi | Name:ecoRVR |
Encoded oni | Plasmid pLB11 Publication Plasmid pLG131 Publication |
Organismi | Escherichia coli |
Taxonomic identifieri | 562 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 70 | N → Q: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 73 | P → A or G: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 74 | D → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 74 | D → E: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 90 | D → A, N, E or T: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 92 | K → E: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 183 – 188 | Missing : Weak, non-specific phosphodiesterase activity. 1 Publication | 6 | |
Mutagenesisi | 183 | S → A or T: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 183 | S → I: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 185 | N → D, A or Q: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 186 | T → S or N: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 187 | T → S or N: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 188 | N → A, Q or T: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 188 | N → D: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 190 | G → A: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 216 – 245 | Missing : Loss of activity. 1 PublicationAdd BLAST | 30 |
Chemistry databases
DrugBanki | DB08651, 3'-THIO-THYMIDINE-5'-PHOSPHATE |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000077305 | 2 – 245 | Type II restriction enzyme EcoRVAdd BLAST | 244 |
Interactioni
Subunit structurei
Homodimer.
1 Publication1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P04390 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04390 |
Family & Domainsi
Family and domain databases
Gene3Di | 3.40.600.10, 1 hit |
InterProi | View protein in InterPro IPR037057, DNA_rep_MutH/RE_typeII_sf IPR011335, Restrct_endonuc-II-like IPR015314, Restrct_endonuc_II_EcoRV IPR019755, Restrct_endonuc_II_EcoRV_Pbac |
Pfami | View protein in Pfam PF09233, Endonuc-EcoRV, 1 hit |
PIRSFi | PIRSF000995, RE_EcoRV, 1 hit |
SUPFAMi | SSF52980, SSF52980, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P04390-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLRSDLINA LYDENQKYDV CGIISAEGKI YPLGSDTKVL STIFELFSRP
60 70 80 90 100
IINKIAEKHG YIVEEPKQQN HYPDFTLYKP SEPNKKIAID IKTTYTNKEN
110 120 130 140 150
EKIKFTLGGY TSFIRNNTKN IVYPFDQYIA HWIIGYVYTR VATRKSSLKT
160 170 180 190 200
YNINELNEIP KPYKGVKVFL QDKWVIAGDL AGSGNTTNIG SIHAHYKDFV
210 220 230 240
EGKGIFDSED EFLDYWRNYE RTSQLRNDKY NNISEYRNWI YRGRK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00530 Genomic DNA Translation: CAA25208.1 M19941 Genomic DNA Translation: AAA24615.1 |
PIRi | A00784, NDECR5 |
RefSeqi | NP_863580.1, NC_005019.1 WP_011117659.1, NZ_VUDS01000061.1 YP_007316617.1, NC_019982.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00530 Genomic DNA Translation: CAA25208.1 M19941 Genomic DNA Translation: AAA24615.1 |
PIRi | A00784, NDECR5 |
RefSeqi | NP_863580.1, NC_005019.1 WP_011117659.1, NZ_VUDS01000061.1 YP_007316617.1, NC_019982.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AZ0 | X-ray | 2.00 | A/B | 2-245 | [»] | |
1AZ3 | X-ray | 2.40 | A/B | 2-245 | [»] | |
1AZ4 | X-ray | 2.40 | A/B | 2-245 | [»] | |
1B94 | X-ray | 1.90 | A/B | 2-245 | [»] | |
1B95 | X-ray | 2.05 | A/B | 2-245 | [»] | |
1B96 | X-ray | 2.30 | A/B | 2-245 | [»] | |
1B97 | X-ray | 1.90 | A/B | 2-245 | [»] | |
1BGB | X-ray | 2.00 | A/B | 2-245 | [»] | |
1BSS | X-ray | 2.15 | A/B | 2-245 | [»] | |
1BSU | X-ray | 2.00 | A/B | 2-245 | [»] | |
1BUA | X-ray | 2.15 | A/B | 2-245 | [»] | |
1EO3 | X-ray | 2.00 | A/B | 1-245 | [»] | |
1EO4 | X-ray | 1.90 | A/B | 1-245 | [»] | |
1EON | X-ray | 1.60 | A/B | 1-245 | [»] | |
1EOO | X-ray | 2.16 | A/B | 1-245 | [»] | |
1EOP | X-ray | 2.60 | A/B | 1-245 | [»] | |
1RV5 | X-ray | 2.10 | A/B | 2-245 | [»] | |
1RVA | X-ray | 2.00 | A/B | 2-245 | [»] | |
1RVB | X-ray | 2.10 | A/B | 2-245 | [»] | |
1RVC | X-ray | 2.10 | A/B | 2-245 | [»] | |
1RVE | X-ray | 2.50 | A/B | 1-245 | [»] | |
1STX | X-ray | 2.10 | A/B | 2-245 | [»] | |
1SUZ | X-ray | 1.80 | A/B | 2-245 | [»] | |
1SX5 | X-ray | 1.50 | A/B | 2-245 | [»] | |
1SX8 | X-ray | 2.15 | A/B | 2-245 | [»] | |
2B0D | X-ray | 2.00 | A/B | 1-245 | [»] | |
2B0E | X-ray | 1.90 | A/B | 1-245 | [»] | |
2GE5 | X-ray | 2.40 | A/B | 2-220 | [»] | |
2RVE | X-ray | 3.00 | A/B | 2-245 | [»] | |
4RVE | X-ray | 3.00 | A/B/C | 2-245 | [»] | |
5F8A | X-ray | 1.76 | A/B | 2-245 | [»] | |
5HLK | X-ray | 2.00 | A/B | 2-245 | [»] | |
SMRi | P04390 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
DrugBanki | DB08651, 3'-THIO-THYMIDINE-5'-PHOSPHATE |
Miscellaneous databases
EvolutionaryTracei | P04390 |
PROi | PR:P04390 |
Family and domain databases
Gene3Di | 3.40.600.10, 1 hit |
InterProi | View protein in InterPro IPR037057, DNA_rep_MutH/RE_typeII_sf IPR011335, Restrct_endonuc-II-like IPR015314, Restrct_endonuc_II_EcoRV IPR019755, Restrct_endonuc_II_EcoRV_Pbac |
Pfami | View protein in Pfam PF09233, Endonuc-EcoRV, 1 hit |
PIRSFi | PIRSF000995, RE_EcoRV, 1 hit |
SUPFAMi | SSF52980, SSF52980, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | T2E5_ECOLX | |
Accessioni | P04390Primary (citable) accession number: P04390 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 1987 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 147 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, PlasmidDocuments
- Restriction enzymes and methylases
Classification of restriction enzymes and methylases and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references