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Protein

Type-2 restriction enzyme EcoRV

Gene

ecoRVR

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GATATC and cleaves after T-3.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Mg2+Note: Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Magnesium 21
Active sitei741
Metal bindingi74Magnesium 11
Metal bindingi74Magnesium 21
Active sitei901
Metal bindingi90Magnesium 11
Active sitei921

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandMagnesium, Metal-binding

Protein family/group databases

REBASEi995 EcoRV

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme EcoRV (EC:3.1.21.4)
Short name:
R.EcoRV
Alternative name(s):
Endonuclease EcoRV
Type II restriction enzyme EcoRV
Gene namesi
Name:ecoRVR
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi70N → Q: Decrease in activity. 1 Publication1
Mutagenesisi73P → A or G: Loss of activity. 1 Publication1
Mutagenesisi74D → A: Loss of activity. 1 Publication1
Mutagenesisi74D → E: Decrease in activity. 1 Publication1
Mutagenesisi90D → A, N, E or T: Loss of activity. 1 Publication1
Mutagenesisi183S → A or T: Decrease in activity. 1 Publication1
Mutagenesisi183S → I: Loss of activity. 1 Publication1
Mutagenesisi185N → D, A or Q: Loss of activity. 1 Publication1
Mutagenesisi186T → S or N: Loss of activity. 1 Publication1
Mutagenesisi187T → S or N: No loss of activity. 1 Publication1
Mutagenesisi188N → A, Q or T: Decrease in activity. 1 Publication1
Mutagenesisi188N → D: Loss of activity. 1 Publication1
Mutagenesisi190G → A: No loss of activity. 1 Publication1

Chemistry databases

DrugBankiDB08651 3'-THIO-THYMIDINE-5'-PHOSPHATE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000773052 – 245Type-2 restriction enzyme EcoRVAdd BLAST244

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1245
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04390
SMRiP04390
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04390

Family & Domainsi

Family and domain databases

Gene3Di3.40.600.10, 1 hit
InterProiView protein in InterPro
IPR037057 DNA_rep_MutH/RE_typeII_sf
IPR011335 Restrct_endonuc-II-like
IPR015314 Restrct_endonuc_II_EcoRV
IPR019755 Restrct_endonuc_II_EcoRV_Pbac
PfamiView protein in Pfam
PF09233 Endonuc-EcoRV, 1 hit
PIRSFiPIRSF000995 RE_EcoRV, 1 hit
SUPFAMiSSF52980 SSF52980, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04390-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLRSDLINA LYDENQKYDV CGIISAEGKI YPLGSDTKVL STIFELFSRP
60 70 80 90 100
IINKIAEKHG YIVEEPKQQN HYPDFTLYKP SEPNKKIAID IKTTYTNKEN
110 120 130 140 150
EKIKFTLGGY TSFIRNNTKN IVYPFDQYIA HWIIGYVYTR VATRKSSLKT
160 170 180 190 200
YNINELNEIP KPYKGVKVFL QDKWVIAGDL AGSGNTTNIG SIHAHYKDFV
210 220 230 240
EGKGIFDSED EFLDYWRNYE RTSQLRNDKY NNISEYRNWI YRGRK
Length:245
Mass (Da):28,650
Last modified:January 23, 2007 - v3
Checksum:i7DB7EC640A746281
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00530 Genomic DNA Translation: CAA25208.1
M19941 Genomic DNA Translation: AAA24615.1
PIRiA00784 NDECR5
RefSeqiNP_863580.1, NC_005019.1
WP_011117659.1, NZ_QITH01000057.1
YP_007316617.1, NC_019982.1

Genome annotation databases

GeneIDi14401263
1446621

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00530 Genomic DNA Translation: CAA25208.1
M19941 Genomic DNA Translation: AAA24615.1
PIRiA00784 NDECR5
RefSeqiNP_863580.1, NC_005019.1
WP_011117659.1, NZ_QITH01000057.1
YP_007316617.1, NC_019982.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AZ0X-ray2.00A/B2-245[»]
1AZ3X-ray2.40A/B2-245[»]
1AZ4X-ray2.40A/B2-245[»]
1B94X-ray1.90A/B2-245[»]
1B95X-ray2.05A/B2-245[»]
1B96X-ray2.30A/B2-245[»]
1B97X-ray1.90A/B2-245[»]
1BGBX-ray2.00A/B2-245[»]
1BSSX-ray2.15A/B2-245[»]
1BSUX-ray2.00A/B2-245[»]
1BUAX-ray2.15A/B2-245[»]
1EO3X-ray2.00A/B1-245[»]
1EO4X-ray1.90A/B1-245[»]
1EONX-ray1.60A/B1-245[»]
1EOOX-ray2.16A/B1-245[»]
1EOPX-ray2.60A/B1-245[»]
1RV5X-ray2.10A/B2-245[»]
1RVAX-ray2.00A/B2-245[»]
1RVBX-ray2.10A/B2-245[»]
1RVCX-ray2.10A/B2-245[»]
1RVEX-ray2.50A/B1-245[»]
1STXX-ray2.10A/B2-245[»]
1SUZX-ray1.80A/B2-245[»]
1SX5X-ray1.50A/B2-245[»]
1SX8X-ray2.15A/B2-245[»]
2B0DX-ray2.00A/B1-245[»]
2B0EX-ray1.90A/B1-245[»]
2GE5X-ray2.40A/B2-220[»]
2RVEX-ray3.00A/B2-245[»]
4RVEX-ray3.00A/B/C2-245[»]
5F8AX-ray1.76A/B2-245[»]
5HLKX-ray2.00A/B2-245[»]
ProteinModelPortaliP04390
SMRiP04390
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB08651 3'-THIO-THYMIDINE-5'-PHOSPHATE

Protein family/group databases

REBASEi995 EcoRV

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi14401263
1446621

Miscellaneous databases

EvolutionaryTraceiP04390

Family and domain databases

Gene3Di3.40.600.10, 1 hit
InterProiView protein in InterPro
IPR037057 DNA_rep_MutH/RE_typeII_sf
IPR011335 Restrct_endonuc-II-like
IPR015314 Restrct_endonuc_II_EcoRV
IPR019755 Restrct_endonuc_II_EcoRV_Pbac
PfamiView protein in Pfam
PF09233 Endonuc-EcoRV, 1 hit
PIRSFiPIRSF000995 RE_EcoRV, 1 hit
SUPFAMiSSF52980 SSF52980, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiT2E5_ECOLX
AccessioniPrimary (citable) accession number: P04390
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: October 10, 2018
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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