UniProtKB - P04355 (MT2_RAT)
Protein
Metallothionein-2
Gene
Mt2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 5 | Divalent metal cation; cluster B | 1 | |
Metal bindingi | 7 | Divalent metal cation; cluster B | 1 | |
Metal bindingi | 13 | Divalent metal cation; cluster B | 1 | |
Metal bindingi | 15 | Divalent metal cation; cluster B | 1 | |
Metal bindingi | 19 | Divalent metal cation; cluster B | 1 | |
Metal bindingi | 21 | Divalent metal cation; cluster B | 1 | |
Metal bindingi | 24 | Divalent metal cation; cluster B | 1 | |
Metal bindingi | 26 | Divalent metal cation; cluster B | 1 | |
Metal bindingi | 29 | Divalent metal cation; cluster B | 1 | |
Metal bindingi | 33 | Divalent metal cation; cluster A | 1 | |
Metal bindingi | 34 | Divalent metal cation; cluster A | 1 | |
Metal bindingi | 36 | Divalent metal cation; cluster A | 1 | |
Metal bindingi | 37 | Divalent metal cation; cluster A | 1 | |
Metal bindingi | 41 | Divalent metal cation; cluster A | 1 | |
Metal bindingi | 44 | Divalent metal cation; cluster A | 1 | |
Metal bindingi | 48 | Divalent metal cation; cluster A | 1 | |
Metal bindingi | 50 | Divalent metal cation; cluster A | 1 | |
Metal bindingi | 57 | Divalent metal cation; cluster A | 1 | |
Metal bindingi | 59 | Divalent metal cation; cluster A | 1 | |
Metal bindingi | 60 | Divalent metal cation; cluster A | 1 |
GO - Molecular functioni
- metal ion binding Source: GO_Central
- zinc ion binding Source: UniProtKB
GO - Biological processi
- cellular response to cadmium ion Source: GO_Central
- cellular response to copper ion Source: GO_Central
- cellular response to zinc ion Source: UniProtKB
- cellular zinc ion homeostasis Source: GO_Central
- detoxification of copper ion Source: GO_Central
- negative regulation of growth Source: UniProtKB
Keywordsi
Ligand | Metal-binding, Metal-thiolate cluster |
Enzyme and pathway databases
Reactomei | R-RNO-5661231, Metallothioneins bind metals |
Names & Taxonomyi
Protein namesi | Recommended name: Metallothionein-2Short name: MT-2 Alternative name(s): Metallothionein-II Short name: MT-II |
Gene namesi | Name:Mt2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197222 | 1 – 61 | Metallothionein-2Add BLAST | 61 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine1 Publication | 1 | |
Modified residuei | 58 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | P04355 |
PTM databases
iPTMneti | P04355 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000043098, Expressed in testis and 21 other tissues |
ExpressionAtlasi | P04355, baseline and differential |
Genevisiblei | P04355, RN |
Interactioni
Protein-protein interaction databases
IntActi | P04355, 1 interactor |
STRINGi | 10116.ENSRNOP00000062585 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P04355 |
SMRi | P04355 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04355 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 29 | BetaAdd BLAST | 29 | |
Regioni | 30 – 61 | AlphaAdd BLAST | 32 |
Domaini
Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4738, Eukaryota |
GeneTreei | ENSGT00950000182967 |
HOGENOMi | CLU_171204_2_0_1 |
InParanoidi | P04355 |
OMAi | CKECKCA |
PhylomeDBi | P04355 |
TreeFami | TF336054 |
Family and domain databases
Gene3Di | 4.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR003019, Metalthion IPR017854, Metalthion_dom_sf IPR023587, Metalthion_dom_sf_vert IPR000006, Metalthion_vert IPR018064, Metalthion_vert_metal_BS |
PANTHERi | PTHR23299, PTHR23299, 1 hit |
Pfami | View protein in Pfam PF00131, Metallothio, 1 hit |
PRINTSi | PR00860, MTVERTEBRATE |
SUPFAMi | SSF57868, SSF57868, 1 hit |
PROSITEi | View protein in PROSITE PS00203, METALLOTHIONEIN_VRT, 1 hit |
i Sequence
Sequence statusi: Complete.
P04355-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDPNCSCATD GSCSCAGSCK CKQCKCTSCK KSCCSCCPVG CAKCSQGCIC
60
KEASDKCSCC A
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11794 Genomic DNA Translation: AAA41640.1 |
PIRi | B61561, SMRT2 |
RefSeqi | NP_001131036.1, NM_001137564.1 |
Genome annotation databases
Ensembli | ENSRNOT00000067391; ENSRNOP00000062585; ENSRNOG00000043098 |
GeneIDi | 689415 |
KEGGi | rno:689415 |
UCSCi | RGD:1592345, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11794 Genomic DNA Translation: AAA41640.1 |
PIRi | B61561, SMRT2 |
RefSeqi | NP_001131036.1, NM_001137564.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MRT | NMR | - | A | 31-61 | [»] | |
2MRT | NMR | - | A | 1-30 | [»] | |
4MT2 | X-ray | 2.00 | A | 1-61 | [»] | |
BMRBi | P04355 | |||||
SMRi | P04355 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P04355, 1 interactor |
STRINGi | 10116.ENSRNOP00000062585 |
PTM databases
iPTMneti | P04355 |
Proteomic databases
PaxDbi | P04355 |
Genome annotation databases
Ensembli | ENSRNOT00000067391; ENSRNOP00000062585; ENSRNOG00000043098 |
GeneIDi | 689415 |
KEGGi | rno:689415 |
UCSCi | RGD:1592345, rat |
Organism-specific databases
CTDi | 4502 |
Phylogenomic databases
eggNOGi | KOG4738, Eukaryota |
GeneTreei | ENSGT00950000182967 |
HOGENOMi | CLU_171204_2_0_1 |
InParanoidi | P04355 |
OMAi | CKECKCA |
PhylomeDBi | P04355 |
TreeFami | TF336054 |
Enzyme and pathway databases
Reactomei | R-RNO-5661231, Metallothioneins bind metals |
Miscellaneous databases
EvolutionaryTracei | P04355 |
PROi | PR:P04355 |
Gene expression databases
Bgeei | ENSRNOG00000043098, Expressed in testis and 21 other tissues |
ExpressionAtlasi | P04355, baseline and differential |
Genevisiblei | P04355, RN |
Family and domain databases
Gene3Di | 4.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR003019, Metalthion IPR017854, Metalthion_dom_sf IPR023587, Metalthion_dom_sf_vert IPR000006, Metalthion_vert IPR018064, Metalthion_vert_metal_BS |
PANTHERi | PTHR23299, PTHR23299, 1 hit |
Pfami | View protein in Pfam PF00131, Metallothio, 1 hit |
PRINTSi | PR00860, MTVERTEBRATE |
SUPFAMi | SSF57868, SSF57868, 1 hit |
PROSITEi | View protein in PROSITE PS00203, METALLOTHIONEIN_VRT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MT2_RAT | |
Accessioni | P04355Primary (citable) accession number: P04355 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 1987 |
Last sequence update: | March 20, 1987 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Metallothioneins
Classification of metallothioneins and list of entries