UniProtKB - P04329 (CAPSD_BBV)

BLAST

>sp|P04329|CAPSD_BBV Capsid protein alpha OS=Black beetle virus OX=12285 GN=alpha PE=1 SV=1
MVRNNNRRRQRTQRIVTTTTQTAPVPQQNVPKQPRRRRNRARRNRRQGRAMNMGALTRLS
QPGLAFLKCAFAPPDFNTDPGKGIPDRFEGKVVTRKDVLNQSINFTANRDTFILIAPTPG
VAYWVADVPAGTFPISTTTFNAVNFPGFNSMFGNAAASRSDQVSSFRYASMNVGIYPTSN
LMQFAGSITVWKCPVKLSNVQFPVATTPATSALVHTLVGLDGVLAVGPDNFSESFIKGVF
SQSVCNEPDFEFSDILEGIQTLPPANVTVATSGQPFNLAAGAEAVSGIVGWGNMDTIVIR
VSAPTGAVNSAILKTWACLEYRPNPNAMLYQFGHDSPPCDEVALQEYRTVARSLPVAVIA
AQNASMWERVKSIIKSSLAMASNVPGPIGIAASGLSGLSALFEGFGF

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History
Entry version 102 (28 Mar 2018)
Sequence version 1 (20 Mar 1987)
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Protein

Capsid protein alpha

Gene

alpha

Organism

Black beetle virus (BBV)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Capsid protein alpha self-assembles to form an icosahedral procapsid with a T=3 symmetry, about 30 nm in diameter, and consisting of 60 capsid proteins trimers. In addition, calcium ions are incorporated per capsid during assembly. The capsid encapsulates the two genomic RNAs. Capsid maturation occurs via autoproteolytic cleavage of capsid protein alpha generating capsid protein beta and the membrane-active peptide gamma (By similarity).By similarity

Peptide gamma is a membrane-disrupting peptide produced by virus maturation, thereby creating the infectious virion. After penetration into the host cell, peptide gamma is probably exposed/released in endosomes, where it disrupts the endosomal membrane, facilitating translocation of viral RNA into the cytoplasm (By similarity).By similarity

Catalytic activityⁱ

Hydrolysis of an asparaginyl bond involved in the maturation of the structural protein of the virus, typically -Asn-|-Ala- or -Asn-|-Phe-.

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	75		1
Metal bindingⁱ	249	CalciumBy similarity	1
Metal bindingⁱ	251	CalciumBy similarity	1

GO - Molecular functionⁱ

aspartic-type endopeptidase activity Source: UniProtKB-KW
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Aspartyl protease, Hydrolase, Protease
Ligand	Calcium, Metal-binding

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	N01.001

MEROPSⁱ

N01.001

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Capsid protein alpha Cleaved into the following 2 chains: Capsid protein beta (EC:3.4.23.44) Alternative name(s): Coat protein beta Nodavirus endopeptidase Peptide gamma Alternative name(s): Coat protein gamma
Gene namesⁱ	Name:alpha
Organismⁱ	Black beetle virus (BBV)
Taxonomic identifierⁱ	12285 [NCBI]
Taxonomic lineageⁱ	Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Nodaviridae › Alphanodavirus
Virus hostⁱ	Heteronychus arator (African black beetle) [TaxID: 295550]
Proteomesⁱ	UP000203003 Componentⁱ: Genome

Subcellular locationⁱ

Capsid protein alpha :

Virion Curated

Capsid protein beta :

Virion Curated

Peptide gamma :

Virion Curated

Note:

Located inside the capsid and probably externalized in early endosomes.Curated

GO - Cellular componentⁱ

T=3 icosahedral viral capsid Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Capsid protein, T=3 icosahedral capsid protein, Virion

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
ChainⁱPRO_0000402386	1 – 407	Capsid protein alphaAdd BLAST	407
ChainⁱPRO_0000039190	1 – 363	Capsid protein betaAdd BLAST	363
ChainⁱPRO_0000039191	364 – 407	Peptide gammaAdd BLAST	44

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Disulfide bondⁱ	69 ↔ 318	By similarity

Post-translational modificationⁱ

Capsid protein alpha autocatalytically maturates into capsid protein beta and peptide gamma.By similarity

Keywords - PTMⁱ

Disulfide bond

Miscellaneous databases

PMAP-CutDBⁱ	P04329

PMAP-CutDBⁱ

P04329

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	61 – 71	Combined sources	11
Helixⁱ	73 – 75	Combined sources	3
Beta strandⁱ	76 – 78	Combined sources	3
Beta strandⁱ	90 – 105	Combined sources	16
Beta strandⁱ	109 – 115	Combined sources	7
Beta strandⁱ	121 – 129	Combined sources	9
Beta strandⁱ	139 – 144	Combined sources	6
Helixⁱ	148 – 152	Combined sources	5
Turnⁱ	160 – 162	Combined sources	3
Beta strandⁱ	163 – 169	Combined sources	7
Beta strandⁱ	172 – 177	Combined sources	6
Turnⁱ	181 – 183	Combined sources	3
Beta strandⁱ	187 – 193	Combined sources	7
Beta strandⁱ	196 – 203	Combined sources	8
Beta strandⁱ	211 – 219	Combined sources	9
Helixⁱ	220 – 224	Combined sources	5
Beta strandⁱ	231 – 234	Combined sources	4
Helixⁱ	235 – 237	Combined sources	3
Beta strandⁱ	239 – 242	Combined sources	4
Beta strandⁱ	247 – 249	Combined sources	3
Beta strandⁱ	260 – 263	Combined sources	4
Helixⁱ	269 – 272	Combined sources	4
Beta strandⁱ	275 – 279	Combined sources	5
Turnⁱ	283 – 285	Combined sources	3
Beta strandⁱ	296 – 303	Combined sources	8
Beta strandⁱ	309 – 323	Combined sources	15
Beta strandⁱ	327 – 329	Combined sources	3
Helixⁱ	330 – 332	Combined sources	3
Helixⁱ	341 – 352	Combined sources	12
Helixⁱ	360 – 362	Combined sources	3
Helixⁱ	367 – 376	Combined sources	10

Show more details

3D structure databases

ProteinModelPortalⁱ	P04329
SMRⁱ	P04329
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P04329

Family & Domainsⁱ

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	17 – 22	Poly-Thr		6
Compositional biasⁱ	35 – 46	Poly-ArgAdd BLAST		12

Sequence similaritiesⁱ

Belongs to the peptidase A6 family.Curated

Family and domain databases

Gene3Dⁱ	2.60.120.20, 1 hit
InterProⁱ	View protein in InterPro IPR000696 Peptidase_A6 IPR029053 Viral_coat
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01829 Peptidase_A6, 1 hit
PRINTSⁱ	PR00863 NODAVIRPTASE

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P04329-1 [UniParc]FASTA Add to basket

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        10         20         30         40         50
MVRNNNRRRQ RTQRIVTTTT QTAPVPQQNV PKQPRRRRNR ARRNRRQGRA 
        60         70         80         90        100
MNMGALTRLS QPGLAFLKCA FAPPDFNTDP GKGIPDRFEG KVVTRKDVLN 
       110        120        130        140        150
QSINFTANRD TFILIAPTPG VAYWVADVPA GTFPISTTTF NAVNFPGFNS 
       160        170        180        190        200
MFGNAAASRS DQVSSFRYAS MNVGIYPTSN LMQFAGSITV WKCPVKLSNV 
       210        220        230        240        250
QFPVATTPAT SALVHTLVGL DGVLAVGPDN FSESFIKGVF SQSVCNEPDF 
       260        270        280        290        300
EFSDILEGIQ TLPPANVTVA TSGQPFNLAA GAEAVSGIVG WGNMDTIVIR 
       310        320        330        340        350
VSAPTGAVNS AILKTWACLE YRPNPNAMLY QFGHDSPPCD EVALQEYRTV 
       360        370        380        390        400
ARSLPVAVIA AQNASMWERV KSIIKSSLAM ASNVPGPIGI AASGLSGLSA 

LFEGFGF

Length:407

Mass (Da):43,838

Last modified:March 20, 1987 - v1

Checksum:ⁱ3A6C6C9A98A5C26C

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X00956 Genomic RNA Translation: CAA25468.1
PIRⁱ	A04151 VCBB2G
NCBI Reference Sequences More...RefSeqⁱ	NP_049329.1, NC_002037.1

Genome annotation databases

GeneIDⁱ	956653
KEGGⁱ	vg:956653

Similar proteinsⁱ

50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P04329	Capsid protein alpha	)	FHV		407	UniRef50_P12870
Capsid protein alpha		BOOLV		403
Putative coat protein		Newington virus		395
Coat protein		Alphanodavirus HB-2007/CHN		407
Coat protein		Drosophila melanogaster American nodavirus (ANV) SW-2009a		407
+8

Cross-referencesⁱ

Web resourcesⁱ

Virus Particle ExploreR db

Icosahedral capsid structure

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X00956 Genomic RNA Translation: CAA25468.1
PIRⁱ	A04151 VCBB2G
RefSeqⁱ	NP_049329.1, NC_002037.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2BBV	X-ray	2.80	A/B/C	1-363	[»]
				D/E/F	364-407	[»]
ProteinModelPortalⁱ	P04329
SMRⁱ	P04329
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein family/group databases

MEROPSⁱ	N01.001

MEROPSⁱ

N01.001

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	956653
KEGGⁱ	vg:956653

Miscellaneous databases

EvolutionaryTraceⁱ	P04329
PMAP-CutDBⁱ	P04329

Family and domain databases

Gene3Dⁱ	2.60.120.20, 1 hit
InterProⁱ	View protein in InterPro IPR000696 Peptidase_A6 IPR029053 Viral_coat
Pfamⁱ	View protein in Pfam PF01829 Peptidase_A6, 1 hit
PRINTSⁱ	PR00863 NODAVIRPTASE
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CAPSD_BBV
Accessionⁱ	P04329Primary (citable) accession number: P04329
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
	Last sequence update:	March 20, 1987
	Last modified:	March 28, 2018
	This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB

UniParc

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UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P04329 (CAPSD_BBV)

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Capsid protein alpha

alpha

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ