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Protein

Capsid protein alpha

Gene

alpha

Organism
Black beetle virus (BBV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Capsid protein alpha self-assembles to form an icosahedral procapsid with a T=3 symmetry, about 30 nm in diameter, and consisting of 60 capsid proteins trimers. In addition, calcium ions are incorporated per capsid during assembly. The capsid encapsulates the two genomic RNAs. Capsid maturation occurs via autoproteolytic cleavage of capsid protein alpha generating capsid protein beta and the membrane-active peptide gamma (By similarity).By similarity
Peptide gamma is a membrane-disrupting peptide produced by virus maturation, thereby creating the infectious virion. After penetration into the host cell, peptide gamma is probably exposed/released in endosomes, where it disrupts the endosomal membrane, facilitating translocation of viral RNA into the cytoplasm (By similarity).By similarity

Catalytic activityi

Hydrolysis of an asparaginyl bond involved in the maturation of the structural protein of the virus, typically -Asn-|-Ala- or -Asn-|-Phe-.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei751
Metal bindingi249CalciumBy similarity1
Metal bindingi251CalciumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
LigandCalcium, Metal-binding

Protein family/group databases

MEROPSiN01.001

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid protein alpha
Cleaved into the following 2 chains:
Alternative name(s):
Coat protein beta
Nodavirus endopeptidase
Alternative name(s):
Coat protein gamma
Gene namesi
Name:alpha
OrganismiBlack beetle virus (BBV)
Taxonomic identifieri12285 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNodaviridaeAlphanodavirus
Virus hostiHeteronychus arator (African black beetle) [TaxID: 295550]
Proteomesi
  • UP000203003 Componenti: Genome

Subcellular locationi

Peptide gamma :
  • Virion Curated
  • Note: Located inside the capsid and probably externalized in early endosomes.Curated

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, T=3 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004023861 – 407Capsid protein alphaAdd BLAST407
ChainiPRO_00000391901 – 363Capsid protein betaAdd BLAST363
ChainiPRO_0000039191364 – 407Peptide gammaAdd BLAST44

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi69 ↔ 318By similarity

Post-translational modificationi

Capsid protein alpha autocatalytically maturates into capsid protein beta and peptide gamma.By similarity

Keywords - PTMi

Disulfide bond

Miscellaneous databases

PMAP-CutDBiP04329

Structurei

Secondary structure

1407
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04329
SMRiP04329
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04329

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi17 – 22Poly-Thr6
Compositional biasi35 – 46Poly-ArgAdd BLAST12

Sequence similaritiesi

Belongs to the peptidase A6 family.Curated

Family and domain databases

Gene3Di2.60.120.20, 1 hit
InterProiView protein in InterPro
IPR000696 Peptidase_A6
IPR029053 Viral_coat
PfamiView protein in Pfam
PF01829 Peptidase_A6, 1 hit
PRINTSiPR00863 NODAVIRPTASE

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04329-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVRNNNRRRQ RTQRIVTTTT QTAPVPQQNV PKQPRRRRNR ARRNRRQGRA
60 70 80 90 100
MNMGALTRLS QPGLAFLKCA FAPPDFNTDP GKGIPDRFEG KVVTRKDVLN
110 120 130 140 150
QSINFTANRD TFILIAPTPG VAYWVADVPA GTFPISTTTF NAVNFPGFNS
160 170 180 190 200
MFGNAAASRS DQVSSFRYAS MNVGIYPTSN LMQFAGSITV WKCPVKLSNV
210 220 230 240 250
QFPVATTPAT SALVHTLVGL DGVLAVGPDN FSESFIKGVF SQSVCNEPDF
260 270 280 290 300
EFSDILEGIQ TLPPANVTVA TSGQPFNLAA GAEAVSGIVG WGNMDTIVIR
310 320 330 340 350
VSAPTGAVNS AILKTWACLE YRPNPNAMLY QFGHDSPPCD EVALQEYRTV
360 370 380 390 400
ARSLPVAVIA AQNASMWERV KSIIKSSLAM ASNVPGPIGI AASGLSGLSA

LFEGFGF
Length:407
Mass (Da):43,838
Last modified:March 20, 1987 - v1
Checksum:i3A6C6C9A98A5C26C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00956 Genomic RNA Translation: CAA25468.1
PIRiA04151 VCBB2G
RefSeqiNP_049329.1, NC_002037.1

Genome annotation databases

GeneIDi956653
KEGGivg:956653

Similar proteinsi

Cross-referencesi

Web resourcesi

Virus Particle ExploreR db

Icosahedral capsid structure

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00956 Genomic RNA Translation: CAA25468.1
PIRiA04151 VCBB2G
RefSeqiNP_049329.1, NC_002037.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BBVX-ray2.80A/B/C1-363[»]
D/E/F364-407[»]
ProteinModelPortaliP04329
SMRiP04329
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiN01.001

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi956653
KEGGivg:956653

Miscellaneous databases

EvolutionaryTraceiP04329
PMAP-CutDBiP04329

Family and domain databases

Gene3Di2.60.120.20, 1 hit
InterProiView protein in InterPro
IPR000696 Peptidase_A6
IPR029053 Viral_coat
PfamiView protein in Pfam
PF01829 Peptidase_A6, 1 hit
PRINTSiPR00863 NODAVIRPTASE
ProtoNetiSearch...

Entry informationi

Entry nameiCAPSD_BBV
AccessioniPrimary (citable) accession number: P04329
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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