UniProtKB - P04329 (CAPSD_BBV)
Capsid protein alpha
alpha
Functioni
Capsid protein alpha self-assembles to form an icosahedral procapsid with a T=3 symmetry, about 30 nm in diameter, and consisting of 60 capsid proteins trimers. In addition, calcium ions are incorporated per capsid during assembly. The capsid encapsulates the two genomic RNAs. Capsid maturation occurs via autoproteolytic cleavage of capsid protein alpha generating capsid protein beta and the membrane-active peptide gamma (By similarity).
By similarityPeptide gamma is a membrane-disrupting peptide produced by virus maturation, thereby creating the infectious virion. After penetration into the host cell, peptide gamma is probably exposed/released in endosomes, where it disrupts the endosomal membrane, facilitating translocation of viral RNA into the cytoplasm (By similarity).
By similarityCatalytic activityi
- Hydrolysis of an asparaginyl bond involved in the maturation of the structural protein of the virus, typically -Asn-|-Ala- or -Asn-|-Phe-. EC:3.4.23.44
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 75 | 1 | ||
Metal bindingi | 249 | CalciumBy similarity | 1 | |
Metal bindingi | 251 | CalciumBy similarity | 1 |
GO - Molecular functioni
- aspartic-type endopeptidase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Aspartyl protease, Hydrolase, Protease |
Ligand | Calcium, Metal-binding |
Protein family/group databases
MEROPSi | N01.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Capsid protein alphaCleaved into the following 2 chains: Alternative name(s): Coat protein beta Nodavirus endopeptidase Alternative name(s): Coat protein gamma |
Gene namesi | Name:alpha |
Organismi | Black beetle virus (BBV) |
Taxonomic identifieri | 12285 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Kitrinoviricota › Magsaviricetes › Nodamuvirales › Nodaviridae › Alphanodavirus |
Virus hosti | Heteronychus arator (African black beetle) [TaxID: 295550] |
Proteomesi |
|
Subcellular locationi
- Virion Curated
- Virion Curated
- Virion Curated Note: Located inside the capsid and probably externalized in early endosomes.Curated
Keywords - Cellular componenti
Capsid protein, T=3 icosahedral capsid protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000402386 | 1 – 407 | Capsid protein alphaAdd BLAST | 407 | |
ChainiPRO_0000039190 | 1 – 363 | Capsid protein betaAdd BLAST | 363 | |
ChainiPRO_0000039191 | 364 – 407 | Peptide gammaAdd BLAST | 44 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 69 ↔ 318 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bondStructurei
Secondary structure
3D structure databases
SMRi | P04329 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04329 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 50 | DisorderedSequence analysisAdd BLAST | 50 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 11 – 29 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 33 – 47 | Basic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 2.60.120.20, 1 hit |
InterProi | View protein in InterPro IPR000696, Peptidase_A6 IPR029053, Viral_coat |
Pfami | View protein in Pfam PF01829, Peptidase_A6, 1 hit |
PRINTSi | PR00863, NODAVIRPTASE |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MVRNNNRRRQ RTQRIVTTTT QTAPVPQQNV PKQPRRRRNR ARRNRRQGRA
60 70 80 90 100
MNMGALTRLS QPGLAFLKCA FAPPDFNTDP GKGIPDRFEG KVVTRKDVLN
110 120 130 140 150
QSINFTANRD TFILIAPTPG VAYWVADVPA GTFPISTTTF NAVNFPGFNS
160 170 180 190 200
MFGNAAASRS DQVSSFRYAS MNVGIYPTSN LMQFAGSITV WKCPVKLSNV
210 220 230 240 250
QFPVATTPAT SALVHTLVGL DGVLAVGPDN FSESFIKGVF SQSVCNEPDF
260 270 280 290 300
EFSDILEGIQ TLPPANVTVA TSGQPFNLAA GAEAVSGIVG WGNMDTIVIR
310 320 330 340 350
VSAPTGAVNS AILKTWACLE YRPNPNAMLY QFGHDSPPCD EVALQEYRTV
360 370 380 390 400
ARSLPVAVIA AQNASMWERV KSIIKSSLAM ASNVPGPIGI AASGLSGLSA
LFEGFGF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00956 Genomic RNA Translation: CAA25468.1 |
PIRi | A04151, VCBB2G |
RefSeqi | NP_049329.1, NC_002037.1 |
Genome annotation databases
GeneIDi | 956653 |
KEGGi | vg:956653 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Virus Particle ExploreR db Icosahedral capsid structure |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00956 Genomic RNA Translation: CAA25468.1 |
PIRi | A04151, VCBB2G |
RefSeqi | NP_049329.1, NC_002037.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2BBV | X-ray | 2.80 | A/B/C | 1-363 | [»] | |
D/E/F | 364-407 | [»] | ||||
SMRi | P04329 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
MEROPSi | N01.001 |
Genome annotation databases
GeneIDi | 956653 |
KEGGi | vg:956653 |
Miscellaneous databases
EvolutionaryTracei | P04329 |
Family and domain databases
Gene3Di | 2.60.120.20, 1 hit |
InterProi | View protein in InterPro IPR000696, Peptidase_A6 IPR029053, Viral_coat |
Pfami | View protein in Pfam PF01829, Peptidase_A6, 1 hit |
PRINTSi | PR00863, NODAVIRPTASE |
MobiDBi | Search... |
Entry informationi
Entry namei | CAPSD_BBV | |
Accessioni | P04329Primary (citable) accession number: P04329 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 1987 |
Last sequence update: | March 20, 1987 | |
Last modified: | June 2, 2021 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families