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Protein

Major DNA-binding protein

Gene

DBP

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays several crucial roles in viral infection. Participates in the opening of the viral DNA origin to initiate replication by interacting with the origin-binding protein. May disrupt loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation. Promotes viral DNA recombination by performing strand-transfer, characterized by the ability to transfer a DNA strand from a linear duplex to a complementary single-stranded DNA circle. Can also catalyze the renaturation of complementary single strands. Additionally, reorganizes the host cell nucleus, leading to the formation of prereplicative sites and replication compartments. This process is driven by the protein which can form double-helical filaments in the absence of DNA.UniRule annotation5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri499 – 512UniRule annotationAdd BLAST14

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-UniRule
  • single-stranded DNA binding Source: UniProtKB-UniRule

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Major DNA-binding proteinUniRule annotation
Gene namesi
Name:DBPUniRule annotation
Synonyms:ICP8
ORF Names:UL29
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome
  • UP000180652 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation2 Publications
  • Note: In the absence of DNA replication, found in the nuclear framework-associated structures (prereplicative sites). As viral DNA replication proceeds, it migrates to globular intranuclear structures (replication compartments).UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001157431 – 1196Major DNA-binding proteinAdd BLAST1196

Proteomic databases

PRIDEiP04296

Interactioni

Subunit structurei

Homooligomers. Forms double-helical filaments necessary for the formation of replication compartments within the host nucleus (PubMed:26676794). Interacts with the origin-binding protein (PubMed:7961904). Interacts with the helicase primase complex; this interaction stimulates primer synthesis activity of the helicase-primase complex (PubMed:9129659). Interacts with the DNA polymerase (PubMed:3031068). Interacts with the alkaline exonuclease; this interaction increases its nuclease processivity (PubMed:15078942). Interacts with ICP27; this interaction plays a role in the stimulation of late gene transcription (PubMed:15582656).UniRule annotation6 Publications

Protein-protein interaction databases

BioGridi971471, 20 interactors
ELMiP04296
IntActiP04296, 53 interactors
MINTiP04296

Structurei

Secondary structure

11196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiP04296
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04296

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1170 – 1196Required for nuclear localizationUniRule annotationAdd BLAST27

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi843 – 844Required for filament formationUniRule annotation2
Motifi1142 – 1144Required for filament formationUniRule annotation3

Sequence similaritiesi

Belongs to the herpesviridae major DNA-binding protein family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri499 – 512UniRule annotationAdd BLAST14

Keywords - Domaini

Zinc-finger

Phylogenomic databases

KOiK19442
OrthoDBiVOG0900000X

Family and domain databases

HAMAPiMF_04007 HSV_DNBI, 1 hit
InterProiView protein in InterPro
IPR035989 DBP_sf
IPR000635 Viral_ssDNA-bd
PfamiView protein in Pfam
PF00747 Viral_DNA_bp, 1 hit
SUPFAMiSSF118208 SSF118208, 1 hit

Sequencei

Sequence statusi: Complete.

P04296-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
METKPKTATT IKVPPGPLGY VYARACPSEG IELLALLSAR SGDSDVAVAP
60 70 80 90 100
LVVGLTVESG FEANVAVVVG SRTTGLGGTA VSLKLTPSHY SSSVYVFHGG
110 120 130 140 150
RHLDPSTQAP NLTRLCERAR RHFGFSDYTP RPGDLKHETT GEALCERLGL
160 170 180 190 200
DPDRALLYLV VTEGFKEAVC INNTFLHLGG SDKVTIGGAE VHRIPVYPLQ
210 220 230 240 250
LFMPDFSRVI AEPFNANHRS IGEKFTYPLP FFNRPLNRLL FEAVVGPAAV
260 270 280 290 300
ALRCRNVDAV ARAAAHLAFD ENHEGAALPA DITFTAFEAS QGKTPRGGRD
310 320 330 340 350
GGGKGAAGGF EQRLASVMAG DAALALESIV SMAVFDEPPT DISAWPLFEG
360 370 380 390 400
QDTAAARANA VGAYLARAAG LVGAMVFSTN SALHLTEVDD AGPADPKDHS
410 420 430 440 450
KPSFYRFFLV PGTHVAANPQ VDREGHVVPG FEGRPTAPLV GGTQEFAGEH
460 470 480 490 500
LAMLCGFSPA LLAKMLFYLE RCDGAVIVGR QEMDVFRYVA DSNQTDVPCN
510 520 530 540 550
LCTFDTRHAC VHTTLMRLRA RHPKFASAAR GAIGVFGTMN SMYSDCDVLG
560 570 580 590 600
NYAAFSALKR ADGSETARTI MQETYRAATE RVMAELETLQ YVDQAVPTAM
610 620 630 640 650
GRLETIITNR EALHTVVNNV RQVVDREVEQ LMRNLVEGRN FKFRDGLGEA
660 670 680 690 700
NHAMSLTLDP YACGPCPLLQ LLGRRSNLAV YQDLALSQCH GVFAGQSVEG
710 720 730 740 750
RNFRNQFQPV LRRRVMDMFN NGFLSAKTLT VALSEGAAIC APSLTAGQTA
760 770 780 790 800
PAESSFEGDV ARVTLGFPKE LRVKSRVLFA GASANASEAA KARVASLQSA
810 820 830 840 850
YQKPDKRVDI LLGPLGFLLK QFHAAIFPNG KPPGSNQPNP QWFWTALQRN
860 870 880 890 900
QLPARLLSRE DIETIAFIKK FSLDYGAINF INLAPNNVSE LAMYYMANQI
910 920 930 940 950
LRYCDHSTYF INTLTAIIAG SRRPPSVQAA AAWSAQGGAG LEAGARALMD
960 970 980 990 1000
AVDAHPGAWT SMFASCNLLR PVMAARPMVV LGLSISKYYG MAGNDRVFQA
1010 1020 1030 1040 1050
GNWASLMGGK NACPLLIFDR TRKFVLACPR AGFVCAASSL GGGAHESSLC
1060 1070 1080 1090 1100
EQLRGIISEG GAAVASSVFV ATVKSLGPRT QQLQIEDWLA LLEDEYLSEE
1110 1120 1130 1140 1150
MMELTARALE RGNGEWSTDA ALEVAHEAEA LVSQLGNAGE VFNFGDFGCE
1160 1170 1180 1190
DDNATPFGGP GAPGPAFAGR KRAFHGDDPF GEGPPDKKGD LTLDML
Length:1,196
Mass (Da):128,350
Last modified:March 20, 1987 - v1
Checksum:i453799162E5B99E9
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti44S → A in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti224K → N in strain: Nonneuroinvasive mutant HF10 and 17 syn+. 1
Natural varianti306A → P in strain: Nonneuroinvasive mutant HF10 and 17 syn+. 1
Natural varianti428V → A in strain: Nonneuroinvasive mutant HF10. 1
Natural varianti475A → G in strain: Nonneuroinvasive mutant HF10 and 17 syn+. 1
Natural varianti964A → T in strain: Nonneuroinvasive mutant HF10. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA Translation: CAA32322.1
X03181 Genomic DNA Translation: CAA26940.1
M21631 Genomic DNA Translation: AAA45787.1
DQ889502 Genomic DNA Translation: ABI63491.1
FJ593289 Genomic DNA Translation: ACM62252.1
PIRiA03790 DNBEV1
RefSeqiYP_009137104.1, NC_001806.2

Genome annotation databases

GeneIDi2703458
KEGGivg:2703458

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14112 Genomic DNA Translation: CAA32322.1
X03181 Genomic DNA Translation: CAA26940.1
M21631 Genomic DNA Translation: AAA45787.1
DQ889502 Genomic DNA Translation: ABI63491.1
FJ593289 Genomic DNA Translation: ACM62252.1
PIRiA03790 DNBEV1
RefSeqiYP_009137104.1, NC_001806.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1URJX-ray3.00A/B1-1136[»]
SMRiP04296
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi971471, 20 interactors
ELMiP04296
IntActiP04296, 53 interactors
MINTiP04296

Proteomic databases

PRIDEiP04296

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703458
KEGGivg:2703458

Phylogenomic databases

KOiK19442
OrthoDBiVOG0900000X

Miscellaneous databases

EvolutionaryTraceiP04296

Family and domain databases

HAMAPiMF_04007 HSV_DNBI, 1 hit
InterProiView protein in InterPro
IPR035989 DBP_sf
IPR000635 Viral_ssDNA-bd
PfamiView protein in Pfam
PF00747 Viral_DNA_bp, 1 hit
SUPFAMiSSF118208 SSF118208, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDNBI_HHV11
AccessioniPrimary (citable) accession number: P04296
Secondary accession number(s): B9VQF7, Q09IA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: November 7, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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