UniProtKB - P04295 (TRM3_HHV11)

BLAST

>sp|P04295|TRM3_HHV11 Tripartite terminase subunit 3 OS=Human herpesvirus 1 (strain 17) OX=10299 GN=TRM3 PE=1 SV=2
MFGQQLASDVQQYLERLEKQRQLKVGADEASAGLTMGGDALRVPFLDFATATPKRHQTVV
PGVGTLHDCCEHSPLFSAVARRLLFNSLVPAQLKGRDFGGDHTAKLEFLAPELVRAVARL
RFKECAPADVVPQRNAYYSVLNTFQALHRSEAFRQLVHFVRDFAQLLKTSFRASSLTETT
GPPKKRAKVDVATHGRTYGTLELFQKMILMHATYFLAAVLLGDHAEQVNTFLRLVFEIPL
FSDAAVRHFRQRATVFLVPRRHGKTWFLVPLIALSLASFRGIKIGYTAHIRKATEPVFEE
IDACLRGWFGSARVDHVKGETISFSFPDGSRSTIVFASSHNTNGIRGQDFNLLFVDEANF
IRPDAVQTIMGFLNQANCKIIFVSSTNTGKASTSFLYNLRGAADELLNVVTYICDDHMPR
VVTHTNATACSCYILNKPVFITMDGAVRRTADLFLADSFMQEIIGGQARETGDDRPVLTK
SAGERFLLYRPSTTTNSGLMAPDLYVYVDPAFTANTRASGTGVAVVGRYRDDYIIFALEH
FFLRALTGSAPADIARCVVHSLTQVLALHPGAFRGVRVAVEGNSSQDSAVAIATHVHTEM
HRLLASEGADAGSGPELLFYHCEPPGSAVLYPFFLLNKQKTPAFEHFIKKFNSGGVMASQ
EIVSATVRLQTDPVEYLLEQLNNLTETVSPNTDVRTYSGKRNGASDDLMVAVIMAIYLAA
QAGPPHTFAPITRVS

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History
Entry version 87 (10 Oct 2018)
Sequence version 2 (01 Jul 1989)
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Protein

Tripartite terminase subunit 3

Gene

TRM3

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Component of the molecular motor that translocates viral genomic DNA in empty capsid during DNA packaging. Forms a tripartite terminase complex together with TRM1 and TRM2 in the host cytoplasm. Once the complex reaches the host nucleus, it interacts with the capsid portal vertex. This portal forms a ring in which genomic DNA is translocated into the capsid. TRM3 carries an RNase H-like nuclease activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genomes.UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	357	For ATPase activityUniRule annotation	1
Active siteⁱ	509	For nuclease activityUniRule annotation1 Publication	1
Active siteⁱ	581	For nuclease activityUniRule annotation1 Publication	1
Active siteⁱ	707	For nuclease activityUniRule annotation1 Publication	1

GO - Molecular functionⁱ

DNA binding Source: UniProtKB-KW
nuclease activity Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA packaging Source: UniProtKB-UniRule
viral release from host cell Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Hydrolase
Biological process	Viral genome packaging, Viral release from host cell

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Tripartite terminase subunit 3UniRule annotation (EC:3.1.-.-UniRule annotation) Alternative name(s): Terminase large subunitUniRule annotation
Gene namesⁱ	Name:TRM3UniRule annotation Ordered Locus Names:UL15
Organismⁱ	Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifierⁱ	10299 [NCBI]
Taxonomic lineageⁱ	Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1
Virus hostⁱ	Homo sapiens (Human) [TaxID: 9606]
Proteomesⁱ	UP000009294 Componentⁱ: Genome

Subcellular locationⁱ

Host nucleus
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_04013
2 Publications
Manual assertion based on experiment inⁱ
- Ref.4
  "Herpes simplex virus 1 DNA packaging proteins encoded by UL6, UL15, UL17, UL28, and UL33 are located on the external surface of the viral capsid."
  Wills E., Scholtes L., Baines J.D.
  J. Virol. 80:10894-10899(2006) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION.
- Ref.5
  "Putative terminase subunits of herpes simplex virus 1 form a complex in the cytoplasm and interact with portal protein in the nucleus."
  Yang K., Homa F., Baines J.D.
  J. Virol. 81:6419-6433(2007) [PubMed] [Europe PMC] [Abstract]
  Cited for: INTERACTION WITH TRM1 AND PORTAL PROTEIN, SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL.

Note:

Responsible for the nuclear localization of the two others subunits TRM1 and TRM2.UniRule annotation1 Publication

GO - Cellular componentⁱ

host cell nucleus Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000115940	1 – 735	Tripartite terminase subunit 3Add BLAST		735

Proteomic databases

PRIDEⁱ	P04295

PRIDEⁱ

P04295

Interactionⁱ

Subunit structureⁱ

Interacts with the terminase subunits TRM1 and TRM2. Interacts with portal protein.UniRule annotation3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
TRM1	P10212	8	EBI-7032965,EBI-7032948
TRM2	P10217	6	EBI-7032965,EBI-7033000

Protein-protein interaction databases

IntActⁱ	P04295, 3 interactors
Molecular INTeraction database More...MINTⁱ	P04295

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	480 – 488	Combined sources	9
Helixⁱ	497 – 499	Combined sources	3
Beta strandⁱ	502 – 509	Combined sources	8
Beta strandⁱ	514 – 516	Combined sources	3
Beta strandⁱ	520 – 529	Combined sources	10
Beta strandⁱ	532 – 542	Combined sources	11
Helixⁱ	550 – 568	Combined sources	19
Beta strandⁱ	574 – 581	Combined sources	8
Helixⁱ	586 – 600	Combined sources	15
Beta strandⁱ	616 – 619	Combined sources	4
Helixⁱ	640 – 652	Combined sources	13
Beta strandⁱ	656 – 663	Combined sources	8
Beta strandⁱ	665 – 671	Combined sources	7
Helixⁱ	673 – 681	Combined sources	9
Helixⁱ	707 – 719	Combined sources	13

Show more details

3D structure databases

ProteinModelPortalⁱ	P04295
SMRⁱ	P04295
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Length
Motifⁱ	183 – 189	Nuclear localization signalUniRule annotation1 Publication	7
Motifⁱ	258 – 265	Walker A motifUniRule annotation	8
Motifⁱ	352 – 357	Walker B motifUniRule annotation	6

Sequence similaritiesⁱ

Belongs to the herpesviridae TRM3 protein family.UniRule annotation

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG0900002F

OrthoDBⁱ

VOG0900002F

Family and domain databases

Gene3Dⁱ	3.30.420.320, 1 hit
HAMAPⁱ	MF_04013 HSV_TRM3, 1 hit
InterProⁱ	View protein in InterPro IPR003498 DNA_pack_C IPR038435 DNA_pack_C_sf IPR003499 DNA_pack_N IPR033663 HSV_TRM3
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02499 DNA_pack_C, 1 hit PF02500 DNA_pack_N, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P04295-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MFGQQLASDV QQYLERLEKQ RQLKVGADEA SAGLTMGGDA LRVPFLDFAT 
        60         70         80         90        100
ATPKRHQTVV PGVGTLHDCC EHSPLFSAVA RRLLFNSLVP AQLKGRDFGG 
       110        120        130        140        150
DHTAKLEFLA PELVRAVARL RFKECAPADV VPQRNAYYSV LNTFQALHRS 
       160        170        180        190        200
EAFRQLVHFV RDFAQLLKTS FRASSLTETT GPPKKRAKVD VATHGRTYGT 
       210        220        230        240        250
LELFQKMILM HATYFLAAVL LGDHAEQVNT FLRLVFEIPL FSDAAVRHFR 
       260        270        280        290        300
QRATVFLVPR RHGKTWFLVP LIALSLASFR GIKIGYTAHI RKATEPVFEE 
       310        320        330        340        350
IDACLRGWFG SARVDHVKGE TISFSFPDGS RSTIVFASSH NTNGIRGQDF 
       360        370        380        390        400
NLLFVDEANF IRPDAVQTIM GFLNQANCKI IFVSSTNTGK ASTSFLYNLR 
       410        420        430        440        450
GAADELLNVV TYICDDHMPR VVTHTNATAC SCYILNKPVF ITMDGAVRRT 
       460        470        480        490        500
ADLFLADSFM QEIIGGQARE TGDDRPVLTK SAGERFLLYR PSTTTNSGLM 
       510        520        530        540        550
APDLYVYVDP AFTANTRASG TGVAVVGRYR DDYIIFALEH FFLRALTGSA 
       560        570        580        590        600
PADIARCVVH SLTQVLALHP GAFRGVRVAV EGNSSQDSAV AIATHVHTEM 
       610        620        630        640        650
HRLLASEGAD AGSGPELLFY HCEPPGSAVL YPFFLLNKQK TPAFEHFIKK 
       660        670        680        690        700
FNSGGVMASQ EIVSATVRLQ TDPVEYLLEQ LNNLTETVSP NTDVRTYSGK 
       710        720        730 
RNGASDDLMV AVIMAIYLAA QAGPPHTFAP ITRVS

Length:735

Mass (Da):80,923

Last modified:July 1, 1989 - v2

Checksum:ⁱA7B8D69C3E6CD965

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X14112 Genomic DNA Translation: CAA32330.1 X03839 Genomic DNA Translation: CAA27456.1 Different termination.
PIRⁱ	A03781 WMBE31 F30083 WMBET5
NCBI Reference Sequences More...RefSeqⁱ	YP_009137089.1, NC_001806.2

Genome annotation databases

GeneIDⁱ	2703385
KEGGⁱ	vg:2703385

Protein	Similar proteins		Species	Score	Length	Source
P04295	Tripartite terminase subunit 3		HHV1		735	UniRef100_P04295
Tripartite terminase subunit 3		HHV11		735
Tripartite terminase subunit 3		Human alphaherpesvirus 1 strain RH2		735
Tripartite terminase subunit 3		HHV1		734
DNA packaging protein (Fragment)		HHV1		123
+2

Protein	Similar proteins		Species	Score	Length	Source
P04295	Tripartite terminase subunit 3		HHV2H		734	UniRef90_P04295
Tripartite terminase subunit 3		HHV1		735
Tripartite terminase subunit 3		HHV11		735
Tripartite terminase subunit 3		Human alphaherpesvirus 1 strain RH2		735
Tripartite terminase subunit 3		HHV2		734
+31

Protein	Similar proteins		Species	Score	Length	Source
P04295	Tripartite terminase subunit 3		HHV2H		734	UniRef50_P04295
Tripartite terminase subunit 3		VZVD		747
Tripartite terminase subunit 3		EHV1B		734
Tripartite terminase subunit 3		EHV1V		734
Tripartite terminase subunit 3		HHV1		735
+121

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X14112 Genomic DNA Translation: CAA32330.1 X03839 Genomic DNA Translation: CAA27456.1 Different termination.
PIRⁱ	A03781 WMBE31 F30083 WMBET5
RefSeqⁱ	YP_009137089.1, NC_001806.2

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4IOX	X-ray	2.46	A/B/C	471-735	[»]
	5HUW	X-ray	1.95	A/B	181-192	[»]
ProteinModelPortalⁱ	P04295
SMRⁱ	P04295
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P04295, 3 interactors
MINTⁱ	P04295

Proteomic databases

PRIDEⁱ	P04295

PRIDEⁱ

P04295

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	2703385
KEGGⁱ	vg:2703385

Phylogenomic databases

OrthoDBⁱ	VOG0900002F

OrthoDBⁱ

VOG0900002F

Family and domain databases

Gene3Dⁱ	3.30.420.320, 1 hit
HAMAPⁱ	MF_04013 HSV_TRM3, 1 hit
InterProⁱ	View protein in InterPro IPR003498 DNA_pack_C IPR038435 DNA_pack_C_sf IPR003499 DNA_pack_N IPR033663 HSV_TRM3
Pfamⁱ	View protein in Pfam PF02499 DNA_pack_C, 1 hit PF02500 DNA_pack_N, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TRM3_HHV11
Accessionⁱ	P04295Primary (citable) accession number: P04295
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
	Last sequence update:	July 1, 1989
	Last modified:	October 10, 2018
	This is version 87 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB

UniParc

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Annotation systems

Supporting data

UniProtKB - P04295 (TRM3_HHV11)

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Tripartite terminase subunit 3

TRM3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ