UniProtKB - P04295 (TRM3_HHV11)
Protein
Tripartite terminase subunit 3
Gene
TRM3
Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Functioni
Component of the molecular motor that translocates viral genomic DNA in empty capsid during DNA packaging. Forms a tripartite terminase complex together with TRM1 and TRM2 in the host cytoplasm. Once the complex reaches the host nucleus, it interacts with the capsid portal vertex. This portal forms a ring in which genomic DNA is translocated into the capsid. TRM3 carries an RNase H-like nuclease activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genomes.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 357 | For ATPase activityUniRule annotation | 1 | |
Active sitei | 509 | For nuclease activityUniRule annotation1 Publication | 1 | |
Active sitei | 581 | For nuclease activityUniRule annotation1 Publication | 1 | |
Active sitei | 707 | For nuclease activityUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- nuclease activity Source: UniProtKB-UniRule
GO - Biological processi
- DNA packaging Source: UniProtKB-UniRule
- viral release from host cell Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding, Hydrolase |
Biological process | Viral genome packaging, Viral release from host cell |
Names & Taxonomyi
Protein namesi | Recommended name: Tripartite terminase subunit 3UniRule annotation (EC:3.1.-.-UniRule annotation)Alternative name(s): Terminase large subunitUniRule annotation |
Gene namesi | Name:TRM3UniRule annotation Ordered Locus Names:UL15 |
Organismi | Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) |
Taxonomic identifieri | 10299 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Peploviricota › Herviviricetes › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Host nucleus UniRule annotation2 Publications Note: Responsible for the nuclear localization of the two others subunits TRM1 and TRM2.UniRule annotation1 Publication
GO - Cellular componenti
- host cell nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host nucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000115940 | 1 – 735 | Tripartite terminase subunit 3Add BLAST | 735 |
Proteomic databases
PRIDEi | P04295 |
Interactioni
Subunit structurei
Interacts with the terminase subunits TRM1 and TRM2.
Interacts with portal protein.
UniRule annotation3 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
IntActi | P04295, 3 interactors |
MINTi | P04295 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P04295 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 183 – 189 | Nuclear localization signalUniRule annotation1 Publication | 7 | |
Motifi | 258 – 265 | Walker A motifUniRule annotation | 8 | |
Motifi | 352 – 357 | Walker B motifUniRule annotation | 6 |
Sequence similaritiesi
Belongs to the herpesviridae TRM3 protein family.UniRule annotation
Family and domain databases
Gene3Di | 3.30.420.320, 1 hit |
HAMAPi | MF_04013, HSV_TRM3, 1 hit |
InterProi | View protein in InterPro IPR003498, DNA_pack_C IPR038435, DNA_pack_C_sf IPR003499, DNA_pack_N IPR033663, HSV_TRM3 |
Pfami | View protein in Pfam PF02499, DNA_pack_C, 1 hit PF02500, DNA_pack_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P04295-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFGQQLASDV QQYLERLEKQ RQLKVGADEA SAGLTMGGDA LRVPFLDFAT
60 70 80 90 100
ATPKRHQTVV PGVGTLHDCC EHSPLFSAVA RRLLFNSLVP AQLKGRDFGG
110 120 130 140 150
DHTAKLEFLA PELVRAVARL RFKECAPADV VPQRNAYYSV LNTFQALHRS
160 170 180 190 200
EAFRQLVHFV RDFAQLLKTS FRASSLTETT GPPKKRAKVD VATHGRTYGT
210 220 230 240 250
LELFQKMILM HATYFLAAVL LGDHAEQVNT FLRLVFEIPL FSDAAVRHFR
260 270 280 290 300
QRATVFLVPR RHGKTWFLVP LIALSLASFR GIKIGYTAHI RKATEPVFEE
310 320 330 340 350
IDACLRGWFG SARVDHVKGE TISFSFPDGS RSTIVFASSH NTNGIRGQDF
360 370 380 390 400
NLLFVDEANF IRPDAVQTIM GFLNQANCKI IFVSSTNTGK ASTSFLYNLR
410 420 430 440 450
GAADELLNVV TYICDDHMPR VVTHTNATAC SCYILNKPVF ITMDGAVRRT
460 470 480 490 500
ADLFLADSFM QEIIGGQARE TGDDRPVLTK SAGERFLLYR PSTTTNSGLM
510 520 530 540 550
APDLYVYVDP AFTANTRASG TGVAVVGRYR DDYIIFALEH FFLRALTGSA
560 570 580 590 600
PADIARCVVH SLTQVLALHP GAFRGVRVAV EGNSSQDSAV AIATHVHTEM
610 620 630 640 650
HRLLASEGAD AGSGPELLFY HCEPPGSAVL YPFFLLNKQK TPAFEHFIKK
660 670 680 690 700
FNSGGVMASQ EIVSATVRLQ TDPVEYLLEQ LNNLTETVSP NTDVRTYSGK
710 720 730
RNGASDDLMV AVIMAIYLAA QAGPPHTFAP ITRVS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14112 Genomic DNA Translation: CAA32330.1 X03839 Genomic DNA Translation: CAA27456.1 Different termination. |
PIRi | A03781, WMBE31 F30083, WMBET5 |
RefSeqi | YP_009137089.1, NC_001806.2 |
Genome annotation databases
GeneIDi | 2703385 |
KEGGi | vg:2703385 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14112 Genomic DNA Translation: CAA32330.1 X03839 Genomic DNA Translation: CAA27456.1 Different termination. |
PIRi | A03781, WMBE31 F30083, WMBET5 |
RefSeqi | YP_009137089.1, NC_001806.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4IOX | X-ray | 2.46 | A/B/C | 471-735 | [»] | |
5HUW | X-ray | 1.95 | A/B | 181-192 | [»] | |
6M5R | electron microscopy | 3.50 | A | 35-728 | [»] | |
6M5S | electron microscopy | 3.90 | A | 35-727 | [»] | |
6M5T | electron microscopy | 2.46 | A | 471-735 | [»] | |
6M5U | electron microscopy | 3.80 | A | 35-728 | [»] | |
6M5V | electron microscopy | 4.50 | A | 38-727 | [»] | |
SMRi | P04295 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P04295, 3 interactors |
MINTi | P04295 |
Proteomic databases
PRIDEi | P04295 |
Genome annotation databases
GeneIDi | 2703385 |
KEGGi | vg:2703385 |
Family and domain databases
Gene3Di | 3.30.420.320, 1 hit |
HAMAPi | MF_04013, HSV_TRM3, 1 hit |
InterProi | View protein in InterPro IPR003498, DNA_pack_C IPR038435, DNA_pack_C_sf IPR003499, DNA_pack_N IPR033663, HSV_TRM3 |
Pfami | View protein in Pfam PF02499, DNA_pack_C, 1 hit PF02500, DNA_pack_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRM3_HHV11 | |
Accessioni | P04295Primary (citable) accession number: P04295 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 1987 |
Last sequence update: | July 1, 1989 | |
Last modified: | February 10, 2021 | |
This is version 95 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families