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Protein

Tropomyosin alpha-1 chain

Gene

TPM1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Muscle protein

Enzyme and pathway databases

ReactomeiR-GGA-390522 Striated Muscle Contraction
R-GGA-445355 Smooth Muscle Contraction

Names & Taxonomyi

Protein namesi
Recommended name:
Tropomyosin alpha-1 chain
Alternative name(s):
Alpha-tropomyosin
Tropomyosin-1
Gene namesi
Name:TPM1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002056251 – 284Tropomyosin alpha-1 chainAdd BLAST284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP04268

PTM databases

iPTMnetiP04268

Expressioni

Gene expression databases

BgeeiENSGALG00000003521 Expressed in 11 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasiP04268 baseline and differential

Interactioni

Subunit structurei

Homodimer (PubMed:23832280). Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain (By similarity). Interacts with HRG (via the HRR domain); the interaction contributes to the antiangiogenic properties of the histidine/proline-rich region (HRR) of HRG (PubMed:15269838, PubMed:15313924).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Smtnl1Q99LM33EBI-8073544,EBI-8073484From Mus musculus.

GO - Molecular functioni

Protein-protein interaction databases

IntActiP04268, 1 interactor
MINTiP04268

Structurei

Secondary structure

1284
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04268
SMRiP04268
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04268

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 284By similarityAdd BLAST284

Domaini

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Sequence similaritiesi

Belongs to the tropomyosin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074494
HOVERGENiHBG107404
InParanoidiP04268
KOiK10373
OMAiQDKADTC
PhylomeDBiP04268
TreeFamiTF351519

Family and domain databases

InterProiView protein in InterPro
IPR000533 Tropomyosin
PfamiView protein in Pfam
PF00261 Tropomyosin, 1 hit
PRINTSiPR00194 TROPOMYOSIN
PROSITEiView protein in PROSITE
PS00326 TROPOMYOSIN, 1 hit

Sequences (6+)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 6 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P04268-1) [UniParc]FASTAAdd to basket
Also known as: Fast-twitch skeletal muscle, CTm7

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEERSKQLE DELVALQKKL
60 70 80 90 100
KGTEDELDKY SESLKDAQEK LELADKKATD AESEVASLNR RIQLVEEELD
110 120 130 140 150
RAQERLATAL QKLEEAEKAA DESERGMKVI ENRAQKDEEK MEIQEIQLKE
160 170 180 190 200
AKHIAEEADR KYEEVARKLV IIEGDLERAE ERAELSESKC AELEEELKTV
210 220 230 240 250
TNNLKSLEAQ AEKYSQKEDK YEEEIKVLTD KLKEAETRAE FAERSVTKLE
260 270 280
KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSI
Length:284
Mass (Da):32,766
Last modified:August 13, 1987 - v2
Checksum:iDBBBD3DB7F36DACB
GO
Isoform 2 (identifier: P04268-2) [UniParc]FASTAAdd to basket
Also known as: Smooth muscle

The sequence of this isoform differs from the canonical sequence as follows:
     42-80: ELVALQKKLK...LELADKKATD → DIVQLEKQLR...LLFAEENAAK
     258-284: DELYAQKLKYKAISEELDHALNDMTSI → EKVAHAKEENLNMHQMLDQTLLELNNM

Show »
Length:284
Mass (Da):32,926
Checksum:iC29E05F9DEA8F27F
GO
Isoform 4 (identifier: P04268-4) [UniParc]FASTAAdd to basket
Also known as: Fibroblast F1

The sequence of this isoform differs from the canonical sequence as follows:
     189-212: KCAELEEELKTVTNNLKSLEAQAE → QVRQLEEQLRIMDQTLKALMAAED
     258-284: DELYAQKLKYKAISEELDHALNDMTSI → EKVAHAKEENLNMHQMLDQTLLELNNM

Show »
Length:284
Mass (Da):32,960
Checksum:iE94405DA8D65597F
GO
Isoform 5 (identifier: P04268-5) [UniParc]FASTAAdd to basket
Also known as: Fibroblast F2

The sequence of this isoform differs from the canonical sequence as follows:
     258-284: DELYAQKLKYKAISEELDHALNDMTSI → EKVAHAKEENLNMHQMLDQTLLELNNM

Show »
Length:284
Mass (Da):32,821
Checksum:iCEA880914631B0DC
GO
Isoform 6 (identifier: P04268-6) [UniParc]FASTAAdd to basket
Also known as: Brain major

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MDAIKKKMQM...LELADKKATD → MAALSSLEAV...VDQERALREE
     259-284: ELYAQKLKYKAISEELDHALNDMTSI → QLYQQLEQNSRLTNELKLALNED

Show »
Length:245
Mass (Da):28,361
Checksum:i0F45465ECA0AD748
GO
Isoform 7 (identifier: P04268-7) [UniParc]FASTAAdd to basket
Also known as: Brain minor

The sequence of this isoform differs from the canonical sequence as follows:
     259-284: ELYAQKLKYKAISEELDHALNDMTSI → QLYQQLEQNSRLTNELKLALNED

Show »
Length:281
Mass (Da):32,502
Checksum:i862297483A4C684C
GO

Computationally mapped potential isoform sequencesi

There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1D5PSX3A0A1D5PSX3_CHICK
Tropomyosin alpha-1 chain
TPM1
321Annotation score:
F1NM23F1NM23_CHICK
Tropomyosin alpha-1 chain
TPM1
284Annotation score:
Q8AWI4Q8AWI4_CHICK
Alpha-tropomyosin 2
TPM1
284Annotation score:
A0A1D5P342A0A1D5P342_CHICK
Tropomyosin alpha-1 chain
TPM1
248Annotation score:
A0A1L1RW88A0A1L1RW88_CHICK
Tropomyosin alpha-1 chain
TPM1
158Annotation score:
A0A1L1RYZ7A0A1L1RYZ7_CHICK
Tropomyosin alpha-1 chain
TPM1
244Annotation score:
A0A1L1RKQ2A0A1L1RKQ2_CHICK
Tropomyosin alpha-1 chain
TPM1
321Annotation score:
A0A1D5NVL7A0A1D5NVL7_CHICK
Tropomyosin alpha-1 chain
TPM1
278Annotation score:
A0A1L1RU52A0A1L1RU52_CHICK
Tropomyosin alpha-1 chain
TPM1
158Annotation score:
A0A1L1RVF2A0A1L1RVF2_CHICK
Tropomyosin alpha-1 chain
TPM1
169Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34E → D AA sequence (PubMed:3997866).Curated1
Sequence conflicti45A → S AA sequence (PubMed:3997866).Curated1
Sequence conflicti63S → A AA sequence (PubMed:3997866).Curated1
Sequence conflicti75D → E AA sequence (PubMed:3997866).Curated1
Sequence conflicti83 – 84SE → AD AA sequence (PubMed:3997866).Curated2
Sequence conflicti132N → S AA sequence (PubMed:3997866).Curated1
Sequence conflicti157E → D AA sequence (PubMed:3997866).Curated1
Sequence conflicti165V → A in AAA48610 (PubMed:3671073).Curated1
Sequence conflicti172I → L AA sequence (PubMed:3997866).Curated1
Sequence conflicti174G → S AA sequence (PubMed:3997866).Curated1
Sequence conflicti188S → G AA sequence (PubMed:3997866).Curated1
Sequence conflicti229T → S AA sequence (PubMed:3997866).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti171I → V. 1
Natural varianti175D → E. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0065871 – 80MDAIK…KKATD → MAALSSLEAVRKKIRSLQEQ ADAAEERAGKLQREVDQERA LREE in isoform 6. CuratedAdd BLAST80
Alternative sequenceiVSP_00658842 – 80ELVAL…KKATD → DIVQLEKQLRVTEDSRDQVL EELHKSEDSLLFAEENAAK in isoform 2. CuratedAdd BLAST39
Alternative sequenceiVSP_006589189 – 212KCAEL…EAQAE → QVRQLEEQLRIMDQTLKALM AAED in isoform 4. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_006590258 – 284DELYA…DMTSI → EKVAHAKEENLNMHQMLDQT LLELNNM in isoform 2, isoform 4 and isoform 5. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_006591259 – 284ELYAQ…DMTSI → QLYQQLEQNSRLTNELKLAL NED in isoform 6 and isoform 7. CuratedAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32441 mRNA Translation: AAA48610.1
X57991
, X57993, X57994, X57995 Genomic DNA Translation: CAA41056.1
M69144
, M69140, M69142, M69143 Genomic DNA Translation: AAA48577.1
X16090 Genomic DNA Translation: CAA34217.1 Sequence problems.
X57991
, X57993, X57994, X57996 Genomic DNA Translation: CAA41059.1
X57991
, X57993, X57994, X57996 Genomic DNA Translation: CAA41058.1
X57991
, X57993, X57994, X57996 Genomic DNA Translation: CAA41057.1
M36336 mRNA Translation: AAA65120.1
M36337 mRNA Translation: AAA65121.1
AY150210 mRNA Translation: AAN75276.1
AADN02040437 Genomic DNA No translation available.
AADN02040438 Genomic DNA No translation available.
J00910 mRNA Translation: AAA49113.1
PIRiS24399 TMCHA
S24400 TMCHS2
S24401
S24402
RefSeqiNP_990732.1, NM_205401.1 [P04268-4]
XP_015134260.1, XM_015278774.1 [P04268-1]
XP_015134264.1, XM_015278778.1 [P04268-5]
XP_015134271.1, XM_015278785.1 [P04268-7]
UniGeneiGga.4108

Genome annotation databases

EnsembliENSGALT00000005572; ENSGALP00000005562; ENSGALG00000003521 [P04268-1]
ENSGALT00000039589; ENSGALP00000038799; ENSGALG00000003521 [P04268-4]
ENSGALT00000060647; ENSGALP00000051797; ENSGALG00000003521 [P04268-5]
GeneIDi396366
KEGGigga:396366

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32441 mRNA Translation: AAA48610.1
X57991
, X57993, X57994, X57995 Genomic DNA Translation: CAA41056.1
M69144
, M69140, M69142, M69143 Genomic DNA Translation: AAA48577.1
X16090 Genomic DNA Translation: CAA34217.1 Sequence problems.
X57991
, X57993, X57994, X57996 Genomic DNA Translation: CAA41059.1
X57991
, X57993, X57994, X57996 Genomic DNA Translation: CAA41058.1
X57991
, X57993, X57994, X57996 Genomic DNA Translation: CAA41057.1
M36336 mRNA Translation: AAA65120.1
M36337 mRNA Translation: AAA65121.1
AY150210 mRNA Translation: AAN75276.1
AADN02040437 Genomic DNA No translation available.
AADN02040438 Genomic DNA No translation available.
J00910 mRNA Translation: AAA49113.1
PIRiS24399 TMCHA
S24400 TMCHS2
S24401
S24402
RefSeqiNP_990732.1, NM_205401.1 [P04268-4]
XP_015134260.1, XM_015278774.1 [P04268-1]
XP_015134264.1, XM_015278778.1 [P04268-5]
XP_015134271.1, XM_015278785.1 [P04268-7]
UniGeneiGga.4108

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IC2X-ray2.00A/B/C/D1-80[»]
3MTUX-ray2.10A/B/C/D1-29[»]
E/F257-284[»]
3MUDX-ray2.20A/B246-257[»]
C/D1-29[»]
3U1AX-ray2.00A/B/C/D1-81[»]
3U1CX-ray1.80A/B1-98[»]
ProteinModelPortaliP04268
SMRiP04268
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04268, 1 interactor
MINTiP04268

PTM databases

iPTMnetiP04268

Proteomic databases

PRIDEiP04268

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000005572; ENSGALP00000005562; ENSGALG00000003521 [P04268-1]
ENSGALT00000039589; ENSGALP00000038799; ENSGALG00000003521 [P04268-4]
ENSGALT00000060647; ENSGALP00000051797; ENSGALG00000003521 [P04268-5]
GeneIDi396366
KEGGigga:396366

Organism-specific databases

CTDi7168

Phylogenomic databases

GeneTreeiENSGT00550000074494
HOVERGENiHBG107404
InParanoidiP04268
KOiK10373
OMAiQDKADTC
PhylomeDBiP04268
TreeFamiTF351519

Enzyme and pathway databases

ReactomeiR-GGA-390522 Striated Muscle Contraction
R-GGA-445355 Smooth Muscle Contraction

Miscellaneous databases

EvolutionaryTraceiP04268
PROiPR:P04268

Gene expression databases

BgeeiENSGALG00000003521 Expressed in 11 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasiP04268 baseline and differential

Family and domain databases

InterProiView protein in InterPro
IPR000533 Tropomyosin
PfamiView protein in Pfam
PF00261 Tropomyosin, 1 hit
PRINTSiPR00194 TROPOMYOSIN
PROSITEiView protein in PROSITE
PS00326 TROPOMYOSIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPM1_CHICK
AccessioniPrimary (citable) accession number: P04268
Secondary accession number(s): P02559
, P08942, P18441, P18442, P49436, P49438, P49439, Q540N4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: September 12, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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