UniProtKB - P04235 (CD3D_MOUSE)
T-cell surface glycoprotein CD3 delta chain
Cd3d
Functioni
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly (PubMed:10935641).
Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.
By similarity1 PublicationGO - Molecular functioni
- identical protein binding Source: MGI
- transmembrane signaling receptor activity Source: GO_Central
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- cell surface receptor signaling pathway Source: GO_Central
- positive thymic T cell selection Source: UniProtKB
- protein-containing complex assembly Source: UniProtKB
- T cell activation Source: UniProtKB
Keywordsi
Molecular function | Receptor |
Biological process | Adaptive immunity, Immunity |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-202424, Downstream TCR signaling R-MMU-202427, Phosphorylation of CD3 and TCR zeta chains R-MMU-202430, Translocation of ZAP-70 to Immunological synapse R-MMU-202433, Generation of second messenger molecules R-MMU-389948, PD-1 signaling R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: T-cell surface glycoprotein CD3 delta chainAlternative name(s): T-cell receptor T3 delta chain CD_antigen: CD3d |
Gene namesi | Name:Cd3d Synonyms:T3d |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88331, Cd3d |
VEuPathDBi | HostDB:ENSMUSG00000032094 |
Subcellular locationi
Other locations
Plasma Membrane
- alpha-beta T cell receptor complex Source: MGI
- external side of plasma membrane Source: GO_Central
- plasma membrane Source: MGI
- T cell receptor complex Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 22 – 105 | ExtracellularSequence analysisAdd BLAST | 84 | |
Transmembranei | 106 – 126 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 127 – 173 | CytoplasmicSequence analysisAdd BLAST | 47 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Add BLAST | 21 | |
ChainiPRO_0000016489 | 22 – 173 | T-cell surface glycoprotein CD3 delta chainAdd BLAST | 152 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 37 ↔ 73 | By similarity | ||
Glycosylationi | 38 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 55 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 74 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 149 | PhosphotyrosinePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 160 | PhosphotyrosinePROSITE-ProRule annotationBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | P04235 |
PaxDbi | P04235 |
PRIDEi | P04235 |
ProteomicsDBi | 279967 |
PTM databases
GlyGeni | P04235, 3 sites |
iPTMneti | P04235 |
PhosphoSitePlusi | P04235 |
SwissPalmi | P04235 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000032094, Expressed in thymus and 98 other tissues |
ExpressionAtlasi | P04235, baseline and differential |
Genevisiblei | P04235, MM |
Interactioni
Subunit structurei
The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta.
Interacts with coreceptors CD4 and CD8.
By similarity1 PublicationGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
CORUMi | P04235 |
ELMi | P04235 |
IntActi | P04235, 1 interactor |
STRINGi | 10090.ENSMUSP00000034602 |
Miscellaneous databases
RNActi | P04235, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 138 – 166 | ITAMPROSITE-ProRule annotationAdd BLAST | 29 |
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S4XC, Eukaryota |
GeneTreei | ENSGT00940000153312 |
HOGENOMi | CLU_115449_0_0_1 |
InParanoidi | P04235 |
OMAi | YQPLRDH |
OrthoDBi | 1466902at2759 |
PhylomeDBi | P04235 |
TreeFami | TF335892 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR015484, CD3_esu/gsu/dsu IPR015485, CD3D IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR032052, Ig_4 IPR003110, Phos_immunorcpt_sig_ITAM |
PANTHERi | PTHR10570, PTHR10570, 1 hit PTHR10570:SF5, PTHR10570:SF5, 1 hit |
Pfami | View protein in Pfam PF16680, Ig_4, 1 hit PF02189, ITAM, 1 hit |
SMARTi | View protein in SMART SM00077, ITAM, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS51055, ITAM_1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MEHSGILASL ILIAVLPQGS PFKIQVTEYE DKVFVTCNTS VMHLDGTVEG
60 70 80 90 100
WFAKNKTLNL GKGVLDPRGI YLCNGTEQLA KVVSSVQVHY RMCQNCVELD
110 120 130 140 150
SGTMAGVIFI DLIATLLLAL GVYCFAGHET GRPSGAAEVQ ALLKNEQLYQ
160 170
PLRDREDTQY SRLGGNWPRN KKS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1L1SV10 | A0A1L1SV10_MOUSE | T-cell surface glycoprotein CD3 del... | Cd3d | 38 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12729, M12728 Genomic DNA Translation: AAA37268.1 M18222 Genomic DNA Translation: AAA37399.1 |
CCDSi | CCDS23124.1 |
PIRi | A02246, RWMSD2 |
RefSeqi | NP_038515.3, NM_013487.3 |
Genome annotation databases
Ensembli | ENSMUST00000034602; ENSMUSP00000034602; ENSMUSG00000032094 |
GeneIDi | 12500 |
KEGGi | mmu:12500 |
UCSCi | uc009pey.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12729, M12728 Genomic DNA Translation: AAA37268.1 M18222 Genomic DNA Translation: AAA37399.1 |
CCDSi | CCDS23124.1 |
PIRi | A02246, RWMSD2 |
RefSeqi | NP_038515.3, NM_013487.3 |
3D structure databases
AlphaFoldDBi | P04235 |
SMRi | P04235 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | P04235 |
ELMi | P04235 |
IntActi | P04235, 1 interactor |
STRINGi | 10090.ENSMUSP00000034602 |
PTM databases
GlyGeni | P04235, 3 sites |
iPTMneti | P04235 |
PhosphoSitePlusi | P04235 |
SwissPalmi | P04235 |
Proteomic databases
EPDi | P04235 |
PaxDbi | P04235 |
PRIDEi | P04235 |
ProteomicsDBi | 279967 |
Protocols and materials databases
ABCDi | P04235, 1 sequenced antibody |
Antibodypediai | 4581, 873 antibodies from 41 providers |
DNASUi | 12500 |
Genome annotation databases
Ensembli | ENSMUST00000034602; ENSMUSP00000034602; ENSMUSG00000032094 |
GeneIDi | 12500 |
KEGGi | mmu:12500 |
UCSCi | uc009pey.2, mouse |
Organism-specific databases
CTDi | 915 |
MGIi | MGI:88331, Cd3d |
VEuPathDBi | HostDB:ENSMUSG00000032094 |
Phylogenomic databases
eggNOGi | ENOG502S4XC, Eukaryota |
GeneTreei | ENSGT00940000153312 |
HOGENOMi | CLU_115449_0_0_1 |
InParanoidi | P04235 |
OMAi | YQPLRDH |
OrthoDBi | 1466902at2759 |
PhylomeDBi | P04235 |
TreeFami | TF335892 |
Enzyme and pathway databases
Reactomei | R-MMU-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-202424, Downstream TCR signaling R-MMU-202427, Phosphorylation of CD3 and TCR zeta chains R-MMU-202430, Translocation of ZAP-70 to Immunological synapse R-MMU-202433, Generation of second messenger molecules R-MMU-389948, PD-1 signaling R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
BioGRID-ORCSi | 12500, 5 hits in 73 CRISPR screens |
ChiTaRSi | Cd3d, mouse |
PROi | PR:P04235 |
RNActi | P04235, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032094, Expressed in thymus and 98 other tissues |
ExpressionAtlasi | P04235, baseline and differential |
Genevisiblei | P04235, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR015484, CD3_esu/gsu/dsu IPR015485, CD3D IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR032052, Ig_4 IPR003110, Phos_immunorcpt_sig_ITAM |
PANTHERi | PTHR10570, PTHR10570, 1 hit PTHR10570:SF5, PTHR10570:SF5, 1 hit |
Pfami | View protein in Pfam PF16680, Ig_4, 1 hit PF02189, ITAM, 1 hit |
SMARTi | View protein in SMART SM00077, ITAM, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit |
PROSITEi | View protein in PROSITE PS51055, ITAM_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CD3D_MOUSE | |
Accessioni | P04235Primary (citable) accession number: P04235 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 1987 |
Last sequence update: | March 20, 1987 | |
Last modified: | May 25, 2022 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot