Cd3d

Functionⁱ

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly (PubMed:10935641). Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.By similarity1 Publication

GO - Molecular functionⁱ

protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI
transcription coactivator activity Source: Ensembl
transmembrane signaling receptor activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adaptive immune response Source: UniProtKB-KW
cell surface receptor signaling pathway Source: InterPro
positive regulation of transcription by RNA polymerase II Source: Ensembl
positive thymic T cell selection
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 9135151
protein-containing complex assembly
Source: UniProtKB
Non-traceable author statementⁱ
- 9135151
protein homooligomerization Source: MGI
T cell activation
Source: UniProtKB
Non-traceable author statementⁱ
- 12215456

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Receptor
Biological process	Adaptive immunity, Immunity

Enzyme and pathway databases

Reactomeⁱ	R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-202424 Downstream TCR signaling R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains R-MMU-202430 Translocation of ZAP-70 to Immunological synapse R-MMU-202433 Generation of second messenger molecules R-MMU-389948 PD-1 signaling R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828 Clathrin-mediated endocytosis

Reactomeⁱ

R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-202424 Downstream TCR signaling
R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains
R-MMU-202430 Translocation of ZAP-70 to Immunological synapse
R-MMU-202433 Generation of second messenger molecules
R-MMU-389948 PD-1 signaling
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: T-cell surface glycoprotein CD3 delta chain Alternative name(s): T-cell receptor T3 delta chain CD_antigen: CD3d
Gene namesⁱ	Name:Cd3d Synonyms:T3d
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 9

Organism-specific databases

MGIⁱ	MGI:88331 Cd3d

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	22 – 105	ExtracellularSequence analysisAdd BLAST	84
Transmembraneⁱ	106 – 126	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	127 – 173	CytoplasmicSequence analysisAdd BLAST	47

Keywords - Cellular componentⁱ

Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Thymocyte development in mice lacking CD3D is arrested at the double-positive stage with markedly diminished TCR expression. Thymocytes are defective in the induction of ERK pathway upon TCR engagement, whereas activation of other MAP kinases is unaffected.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 21	Add BLAST		21
ChainⁱPRO_0000016489	22 – 173	T-cell surface glycoprotein CD3 delta chainAdd BLAST		152

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	37 ↔ 73	By similarity
Glycosylationⁱ	38	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	55	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	74	N-linked (GlcNAc...) asparagineSequence analysis	1
Modified residueⁱ	149	PhosphotyrosinePROSITE-ProRule annotationBy similarity	1
Modified residueⁱ	160	PhosphotyrosinePROSITE-ProRule annotationBy similarity	1

Post-translational modificationⁱ

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.By similarity

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDⁱ	P04235
PaxDbⁱ	P04235
PRIDEⁱ	P04235

PTM databases

iPTMnetⁱ	P04235
PhosphoSitePlusⁱ	P04235
SwissPalmⁱ	P04235

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000032094 Expressed in 65 organ(s), highest expression level in thymus
CleanExⁱ	MM_CD3D
ExpressionAtlasⁱ	P04235 baseline and differential
Genevisibleⁱ	P04235 MM

Interactionⁱ

Subunit structureⁱ

The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with coreceptors CD4 and CD8.By similarity1 Publication

GO - Molecular functionⁱ

protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

CORUMⁱ	P04235
ELMⁱ	P04235
IntActⁱ	P04235, 1 interactor
STRINGⁱ	10090.ENSMUSP00000034602

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P04235
SMRⁱ	P04235
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	138 – 166	ITAMPROSITE-ProRule annotationAdd BLAST		29

Keywords - Domainⁱ

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IWAX Eukaryota ENOG410Y2TD LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046930
HOGENOMⁱ	HOG000015287
HOVERGENⁱ	HBG005278
InParanoidⁱ	P04235
KEGG Orthology (KO) More...KOⁱ	K06450
OMAⁱ	MCQSCVE
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0U7X
PhylomeDBⁱ	P04235
TreeFamⁱ	TF335892

Family and domain databases

Gene3Dⁱ	2.60.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR015484 CD3_esu/gsu/dsu IPR015485 CD3D IPR036179 Ig-like_dom_sf IPR013783 Ig-like_fold IPR032052 Ig_4 IPR003110 Phos_immunorcpt_sig_ITAM
PANTHERⁱ	PTHR10570 PTHR10570, 1 hit PTHR10570:SF5 PTHR10570:SF5, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF16680 Ig_4, 1 hit PF02189 ITAM, 1 hit
SMARTⁱ	View protein in SMART SM00077 ITAM, 1 hit
SUPFAMⁱ	SSF48726 SSF48726, 1 hit
PROSITEⁱ	View protein in PROSITE PS51055 ITAM_1, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

P04235-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEHSGILASL ILIAVLPQGS PFKIQVTEYE DKVFVTCNTS VMHLDGTVEG 
        60         70         80         90        100
WFAKNKTLNL GKGVLDPRGI YLCNGTEQLA KVVSSVQVHY RMCQNCVELD 
       110        120        130        140        150
SGTMAGVIFI DLIATLLLAL GVYCFAGHET GRPSGAAEVQ ALLKNEQLYQ 
       160        170 
PLRDREDTQY SRLGGNWPRN KKS

Length:173

Mass (Da):19,032

Last modified:March 20, 1987 - v1

Checksum:ⁱEEEC182BDF2AFB6B

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A1L1SV10	A0A1L1SV10_MOUSE		T-cell surface glycoprotein CD3 del... T-cell surface glycoprotein CD3 delta chain	Cd3d	38	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M12729, M12728 Genomic DNA Translation: AAA37268.1 M18222 Genomic DNA Translation: AAA37399.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS23124.1
PIRⁱ	A02246 RWMSD2
NCBI Reference Sequences More...RefSeqⁱ	NP_038515.3, NM_013487.3
UniGeneⁱ	Mm.4527

Genome annotation databases

Ensemblⁱ	ENSMUST00000034602; ENSMUSP00000034602; ENSMUSG00000032094
GeneIDⁱ	12500
KEGGⁱ	mmu:12500
UCSC genome browser More...UCSCⁱ	uc009pey.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P04235	CD3 antigen delta polypeptide		MOUSE		173	UniRef100_P04235
T3 delta-chain (Fragment)		MOUSE		162

Protein	Similar proteins		Species	Score	Length	Source
P04235	CD3 antigen delta polypeptide		MOUSE		173	UniRef90_P04235
UPI000A31A114		MUSCR		173
T3 delta-chain (Fragment)		MOUSE		162
Uncharacterized protein		MOUSE		173
CD3 antigen delta polypeptide		MOUSE		173
+2

Protein	Similar proteins		Species	Score	Length	Source
P04235	T-cell surface glycoprotein CD3 delta chain	)	SHEEP		167	UniRef50_P04235
T-cell surface glycoprotein CD3 delta chain		PIG		171
T-cell surface glycoprotein CD3 delta chain		MACFA		171
T-cell surface glycoprotein CD3 delta chain		BOVIN		168
T-cell surface glycoprotein CD3 delta chain		RAT		173
+79

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M12729, M12728 Genomic DNA Translation: AAA37268.1 M18222 Genomic DNA Translation: AAA37399.1
CCDSⁱ	CCDS23124.1
PIRⁱ	A02246 RWMSD2
RefSeqⁱ	NP_038515.3, NM_013487.3
UniGeneⁱ	Mm.4527

3D structure databases

ProteinModelPortalⁱ	P04235
SMRⁱ	P04235
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

CORUMⁱ	P04235
ELMⁱ	P04235
IntActⁱ	P04235, 1 interactor
STRINGⁱ	10090.ENSMUSP00000034602

PTM databases

iPTMnetⁱ	P04235
PhosphoSitePlusⁱ	P04235
SwissPalmⁱ	P04235

Proteomic databases

EPDⁱ	P04235
PaxDbⁱ	P04235
PRIDEⁱ	P04235

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000034602; ENSMUSP00000034602; ENSMUSG00000032094
GeneIDⁱ	12500
KEGGⁱ	mmu:12500
UCSCⁱ	uc009pey.2 mouse

Organism-specific databases

CTDⁱ	915
MGIⁱ	MGI:88331 Cd3d

Phylogenomic databases

eggNOGⁱ	ENOG410IWAX Eukaryota ENOG410Y2TD LUCA
GeneTreeⁱ	ENSGT00510000046930
HOGENOMⁱ	HOG000015287
HOVERGENⁱ	HBG005278
InParanoidⁱ	P04235
KOⁱ	K06450
OMAⁱ	MCQSCVE
OrthoDBⁱ	EOG091G0U7X
PhylomeDBⁱ	P04235
TreeFamⁱ	TF335892

Enzyme and pathway databases

Reactomeⁱ	R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-202424 Downstream TCR signaling R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains R-MMU-202430 Translocation of ZAP-70 to Immunological synapse R-MMU-202433 Generation of second messenger molecules R-MMU-389948 PD-1 signaling R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828 Clathrin-mediated endocytosis

Reactomeⁱ

R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-202424 Downstream TCR signaling
R-MMU-202427 Phosphorylation of CD3 and TCR zeta chains
R-MMU-202430 Translocation of ZAP-70 to Immunological synapse
R-MMU-202433 Generation of second messenger molecules
R-MMU-389948 PD-1 signaling
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P04235
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000032094 Expressed in 65 organ(s), highest expression level in thymus
CleanExⁱ	MM_CD3D
ExpressionAtlasⁱ	P04235 baseline and differential
Genevisibleⁱ	P04235 MM

Family and domain databases

Gene3Dⁱ	2.60.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR015484 CD3_esu/gsu/dsu IPR015485 CD3D IPR036179 Ig-like_dom_sf IPR013783 Ig-like_fold IPR032052 Ig_4 IPR003110 Phos_immunorcpt_sig_ITAM
PANTHERⁱ	PTHR10570 PTHR10570, 1 hit PTHR10570:SF5 PTHR10570:SF5, 1 hit
Pfamⁱ	View protein in Pfam PF16680 Ig_4, 1 hit PF02189 ITAM, 1 hit
SMARTⁱ	View protein in SMART SM00077 ITAM, 1 hit
SUPFAMⁱ	SSF48726 SSF48726, 1 hit
PROSITEⁱ	View protein in PROSITE PS51055 ITAM_1, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CD3D_MOUSE
Accessionⁱ	P04235Primary (citable) accession number: P04235
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
	Last sequence update:	March 20, 1987
	Last modified:	September 12, 2018
	This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P04235 (CD3D_MOUSE)

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T-cell surface glycoprotein CD3 delta chain

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ