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Protein

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

Gene

ATP2A1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Activity regulationi

Inhibited by sarcolipin (SLN) and myoregulin (MRLN) (PubMed:10551848, PubMed:8428955). Inhibited by phospholamban (PLN) (PubMed:10551848, PubMed:8428955). Reversibly inhibited by phospholamban (PLN) at low calcium concentrations (PubMed:10551848, PubMed:8428955). Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium (PubMed:10551848, PubMed:8428955). This inhibition is regulated by the phosphorylation of PLN (PubMed:10551848, PubMed:8428955). Enhanced by DWORF; DWORF increases activity by displacing sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN) (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi304Calcium 2; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi305Calcium 2; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi307Calcium 2; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi309Calcium 2Combined sources1 Publication1
Active sitei3514-aspartylphosphate intermediate1 Publication1
Metal bindingi703MagnesiumBy similarity1
Metal bindingi707MagnesiumBy similarity1
Metal bindingi768Calcium 1Combined sources1 Publication1
Metal bindingi771Calcium 1Combined sources1 Publication1
Metal bindingi796Calcium 2Combined sources1 Publication1
Metal bindingi799Calcium 1Combined sources1 Publication1
Metal bindingi800Calcium 1Combined sources1 Publication1
Metal bindingi800Calcium 2Combined sources1 Publication1
Metal bindingi908Calcium 1Combined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.8 1749
SABIO-RKiP04191

Protein family/group databases

TCDBi3.A.3.2.43 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (EC:3.6.3.8)
Short name:
SERCA1
Short name:
SR Ca(2+)-ATPase 1
Alternative name(s):
Calcium pump 1
Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform
Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Gene namesi
Name:ATP2A1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48CytoplasmicAdd BLAST48
Transmembranei49 – 69Helical; Name=1Add BLAST21
Topological domaini70 – 89LumenalAdd BLAST20
Transmembranei90 – 110Helical; Name=2Add BLAST21
Topological domaini111 – 253CytoplasmicAdd BLAST143
Transmembranei254 – 273Helical; Name=3Add BLAST20
Topological domaini274 – 295LumenalAdd BLAST22
Transmembranei296 – 313Helical; Name=4Add BLAST18
Topological domaini314 – 757CytoplasmicAdd BLAST444
Transmembranei758 – 777Helical; Name=5Add BLAST20
Topological domaini778 – 787Lumenal10
Transmembranei788 – 808Helical; Name=6Add BLAST21
Topological domaini809 – 828CytoplasmicAdd BLAST20
Transmembranei829 – 851Helical; Name=7Add BLAST23
Topological domaini852 – 897LumenalAdd BLAST46
Transmembranei898 – 917Helical; Name=8Add BLAST20
Topological domaini918 – 930CytoplasmicAdd BLAST13
Transmembranei931 – 949Helical; Name=9Add BLAST19
Topological domaini950 – 964LumenalAdd BLAST15
Transmembranei965 – 985Helical; Name=10Add BLAST21
Topological domaini986 – 1001CytoplasmicAdd BLAST16

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4693

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000461891 – 1001Sarcoplasmic/endoplasmic reticulum calcium ATPase 1Add BLAST1001

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei441PhosphothreonineBy similarity1
Modified residuei569PhosphothreonineBy similarity1
Modified residuei581PhosphoserineBy similarity1
Disulfide bondi876 ↔ 8881 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PRIDEiP04191

PTM databases

SwissPalmiP04191

Expressioni

Tissue specificityi

Skeletal muscle, fast twitch muscle (type II) fibers.1 Publication

Developmental stagei

Isoform SERCA1A and isoform SERCA1B are predominantly found in adult and neonatal skeletal muscle respectively.1 Publication

Inductioni

Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression.

Interactioni

Subunit structurei

Interacts with sarcolipin (SLN) (By similarity). Interacts with phospholamban (PLN) (PubMed:10551848, PubMed:8428955). Interacts with myoregulin (MRLN) (By similarity). Interacts with DWORF (By similarity).By similarity2 Publications

Protein-protein interaction databases

IntActiP04191, 5 interactors
MINTiP04191
STRINGi9986.ENSOCUP00000002327

Chemistry databases

BindingDBiP04191

Structurei

Secondary structure

11001
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04191
SMRiP04191
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04191

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni370 – 400Interaction with phospholamban 11 PublicationAdd BLAST31
Regioni788 – 808Interaction with phospholamban 21 PublicationAdd BLAST21

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0202 Eukaryota
COG0474 LUCA
HOVERGENiHBG105648
InParanoidiP04191
KOiK05853

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR005782 P-type_ATPase_IIA
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01116 ATPase-IIA1_Ca, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform SERCA1B (identifier: P04191-1) [UniParc]FASTAAdd to basket
Also known as: Neonatal

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEAAHSKSTE ECLAYFGVSE TTGLTPDQVK RHLEKYGHNE LPAEEGKSLW
60 70 80 90 100
ELVIEQFEDL LVRILLLAAC ISFVLAWFEE GEETITAFVE PFVILLILIA
110 120 130 140 150
NAIVGVWQER NAENAIEALK EYEPEMGKVY RADRKSVQRI KARDIVPGDI
160 170 180 190 200
VEVAVGDKVP ADIRILSIKS TTLRVDQSIL TGESVSVIKH TEPVPDPRAV
210 220 230 240 250
NQDKKNMLFS GTNIAAGKAL GIVATTGVST EIGKIRDQMA ATEQDKTPLQ
260 270 280 290 300
QKLDEFGEQL SKVISLICVA VWLINIGHFN DPVHGGSWIR GAIYYFKIAV
310 320 330 340 350
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS
360 370 380 390 400
DKTGTLTTNQ MSVCKMFIID KVDGDFCSLN EFSITGSTYA PEGEVLKNDK
410 420 430 440 450
PIRSGQFDGL VELATICALC NDSSLDFNET KGVYEKVGEA TETALTTLVE
460 470 480 490 500
KMNVFNTEVR NLSKVERANA CNSVIRQLMK KEFTLEFSRD RKSMSVYCSP
510 520 530 540 550
AKSSRAAVGN KMFVKGAPEG VIDRCNYVRV GTTRVPMTGP VKEKILSVIK
560 570 580 590 600
EWGTGRDTLR CLALATRDTP PKREEMVLDD SSRFMEYETD LTFVGVVGML
610 620 630 640 650
DPPRKEVMGS IQLCRDAGIR VIMITGDNKG TAIAICRRIG IFGENEEVAD
660 670 680 690 700
RAYTGREFDD LPLAEQREAC RRACCFARVE PSHKSKIVEY LQSYDEITAM
710 720 730 740 750
TGDGVNDAPA LKKAEIGIAM GSGTAVAKTA SEMVLADDNF STIVAAVEEG
760 770 780 790 800
RAIYNNMKQF IRYLISSNVG EVVCIFLTAA LGLPEALIPV QLLWVNLVTD
810 820 830 840 850
GLPATALGFN PPDLDIMDRP PRSPKEPLIS GWLFFRYMAI GGYVGAATVG
860 870 880 890 900
AAAWWFMYAE DGPGVTYHQL THFMQCTEDH PHFEGLDCEI FEAPEPMTMA
910 920 930 940 950
LSVLVTIEMC NALNSLSENQ SLMRMPPWVN IWLLGSICLS MSLHFLILYV
960 970 980 990 1000
DPLPMIFKLK ALDLTQWLMV LKISLPVIGL DEILKFIARN YLEDPEDERR

K
Length:1,001
Mass (Da):110,459
Last modified:March 20, 1987 - v1
Checksum:i1F0D8C36CF975266
GO
Isoform SERCA1A (identifier: P04191-2) [UniParc]FASTAAdd to basket
Also known as: Adult

The sequence of this isoform differs from the canonical sequence as follows:
     994-1001: DPEDERRK → G

Show »
Length:994
Mass (Da):109,490
Checksum:i148C462A8653A3E5
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000356994 – 1001DPEDERRK → G in isoform SERCA1A. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12898 mRNA Translation: AAA31165.1
M15351 mRNA Translation: AAA31166.1
M15158 mRNA Translation: AAA31167.1
PIRiA01075 PWRBFC
RefSeqiNP_001082787.1, NM_001089318.1 [P04191-1]
UniGeneiOcu.1829
Ocu.6937

Genome annotation databases

GeneIDi100037716
KEGGiocu:100037716

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12898 mRNA Translation: AAA31165.1
M15351 mRNA Translation: AAA31166.1
M15158 mRNA Translation: AAA31167.1
PIRiA01075 PWRBFC
RefSeqiNP_001082787.1, NM_001089318.1 [P04191-1]
UniGeneiOcu.1829
Ocu.6937

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FQUmodel-A1-993[»]
1IWOX-ray3.10A/B1-994[»]
1KJUelectron microscopy6.00A1-994[»]
1SU4X-ray2.40A1-994[»]
1T5SX-ray2.60A1-993[»]
1T5TX-ray2.90A1-993[»]
1VFPX-ray2.90A/B1-994[»]
1WPGX-ray2.30A/B/C/D1-993[»]
1XP5X-ray3.00A1-993[»]
2AGVX-ray2.40A/B1-993[»]
2BY4X-ray3.30A1-993[»]
2C88X-ray3.10A1-993[»]
2C8KX-ray2.80A1-993[»]
2C8LX-ray3.10A1-993[»]
2C9MX-ray3.00A/B1-993[»]
2DQSX-ray2.50A1-993[»]
2EARX-ray3.10A1-993[»]
2EATX-ray2.90A1-993[»]
2EAUX-ray2.80A1-993[»]
2O9JX-ray2.65A1-993[»]
2OA0X-ray3.40A1-993[»]
2VOYelectron microscopy18.00B36-77[»]
C967-988[»]
D832-854[»]
E86-115[»]
G243-278[»]
H289-336[»]
K749-780[»]
L789-809[»]
2YFYX-ray3.10A1-993[»]
2ZBDX-ray2.40A1-993[»]
2ZBEX-ray3.80A/B1-993[»]
2ZBFX-ray2.40A1-993[»]
2ZBGX-ray2.55A1-993[»]
3AR2X-ray2.50A1-994[»]
3AR3X-ray2.30A1-994[»]
3AR4X-ray2.15A1-994[»]
3AR5X-ray2.20A1-994[»]
3AR6X-ray2.20A1-994[»]
3AR7X-ray2.15A1-994[»]
3AR8X-ray2.60A1-994[»]
3AR9X-ray2.60A1-994[»]
3B9BX-ray2.65A1-993[»]
3B9RX-ray3.00A/B1-993[»]
3BA6X-ray2.80A1-993[»]
3FGOX-ray2.50A/B1-994[»]
3FPBX-ray2.55A1-994[»]
3FPSX-ray3.20A1-994[»]
3J7Telectron microscopy3.40A1-994[»]
3N5KX-ray2.20A/B1-994[»]
3N8GX-ray2.58A1-993[»]
3W5AX-ray3.01A/B1-993[»]
3W5BX-ray3.20A1-993[»]
3W5CX-ray2.50A1-993[»]
3W5DX-ray2.45A1-993[»]
4BEWX-ray2.50A/B1-994[»]
4H1WX-ray3.10A1-993[»]
4J2TX-ray3.20A1-993[»]
4KYTX-ray2.83A1-993[»]
4NABX-ray3.50A1-993[»]
4UU0X-ray2.50A1-993[»]
4UU1X-ray2.80A1-993[»]
4XOUX-ray2.80A1-993[»]
4Y3UX-ray3.51A1-993[»]
4YCLX-ray3.25A1-993[»]
4YCMX-ray3.20A2-993[»]
4YCNX-ray3.50A2-993[»]
5A3QX-ray3.05A1-993[»]
5A3RX-ray3.05A1-993[»]
5A3SX-ray3.30A/B1-993[»]
5NCQX-ray3.00A1-993[»]
5XA7X-ray3.20A1-993[»]
5XA8X-ray3.20A1-993[»]
5XA9X-ray3.20A1-993[»]
5XAAX-ray3.20A1-993[»]
5XABX-ray3.20A1-993[»]
6HEFX-ray3.54A1-993[»]
ProteinModelPortaliP04191
SMRiP04191
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04191, 5 interactors
MINTiP04191
STRINGi9986.ENSOCUP00000002327

Chemistry databases

BindingDBiP04191
ChEMBLiCHEMBL4693

Protein family/group databases

TCDBi3.A.3.2.43 the p-type atpase (p-atpase) superfamily

PTM databases

SwissPalmiP04191

Proteomic databases

PRIDEiP04191

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100037716
KEGGiocu:100037716

Organism-specific databases

CTDi487

Phylogenomic databases

eggNOGiKOG0202 Eukaryota
COG0474 LUCA
HOVERGENiHBG105648
InParanoidiP04191
KOiK05853

Enzyme and pathway databases

BRENDAi3.6.3.8 1749
SABIO-RKiP04191

Miscellaneous databases

EvolutionaryTraceiP04191

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR005782 P-type_ATPase_IIA
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01116 ATPase-IIA1_Ca, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAT2A1_RABIT
AccessioniPrimary (citable) accession number: P04191
Secondary accession number(s): P11719
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: October 10, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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