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Protein

Subtilisin E

Gene

aprE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.

Miscellaneous

Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation.

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.

Cofactori

Ca2+1 PublicationNote: Binds 2 calcium ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108Calcium 11 Publication1
Active sitei138Charge relay system1 Publication1
Metal bindingi147Calcium 11 Publication1
Active sitei170Charge relay system1 Publication1
Metal bindingi181Calcium 1; via carbonyl oxygen1 Publication1
Metal bindingi183Calcium 11 Publication1
Metal bindingi185Calcium 1; via carbonyl oxygen1 Publication1
Metal bindingi187Calcium 1; via carbonyl oxygen1 Publication1
Metal bindingi275Calcium 2; via carbonyl oxygen1 Publication1
Metal bindingi277Calcium 2; via carbonyl oxygen1 Publication1
Metal bindingi280Calcium 21 Publication1
Metal bindingi303Calcium 21 Publication1
Active sitei327Charge relay system1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processSporulation
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU10300-MONOMER
SABIO-RKiP04189

Protein family/group databases

MEROPSiS08.036

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin E (EC:3.4.21.62)
Gene namesi
Name:aprE
Synonyms:apr, aprA, sprE
Ordered Locus Names:BSU10300
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi138D → N: Prevents intramolecular processing to an active enzyme. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
PropeptideiPRO_000002718730 – 106Add BLAST77
ChainiPRO_0000027188107 – 381Subtilisin EAdd BLAST275

Keywords - PTMi

Autocatalytic cleavage, Zymogen

Proteomic databases

PaxDbiP04189
PRIDEiP04189

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005716

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04189
SMRiP04189
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04189

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 379Peptidase S8Add BLAST247

Domaini

The propeptide functions as an intramolecular chaperone which is essential for the correct folding of the protease domain but is not required for enzymatic function of the folded protein. It is autoprocessed and degraded after completion of the folding process.1 Publication

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105RX7 Bacteria
COG1404 LUCA
HOGENOMiHOG000199176
InParanoidiP04189
KOiK01342
OMAiSGQYDWI
PhylomeDBiP04189

Family and domain databases

CDDicd07477 Peptidases_S8_Subtilisin_subse, 1 hit
Gene3Di3.30.70.80, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR010259 S8pro/Inhibitor_I9
IPR037045 S8pro/Inhibitor_I9_sf
IPR034202 Subtilisin_Carlsberg-like
PfamiView protein in Pfam
PF05922 Inhibitor_I9, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04189-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRSKKLWISL LFALTLIFTM AFSNMSAQAA GKSSTEKKYI VGFKQTMSAM
60 70 80 90 100
SSAKKKDVIS EKGGKVQKQF KYVNAAAATL DEKAVKELKK DPSVAYVEED
110 120 130 140 150
HIAHEYAQSV PYGISQIKAP ALHSQGYTGS NVKVAVIDSG IDSSHPDLNV
160 170 180 190 200
RGGASFVPSE TNPYQDGSSH GTHVAGTIAA LNNSIGVLGV APSASLYAVK
210 220 230 240 250
VLDSTGSGQY SWIINGIEWA ISNNMDVINM SLGGPTGSTA LKTVVDKAVS
260 270 280 290 300
SGIVVAAAAG NEGSSGSTST VGYPAKYPST IAVGAVNSSN QRASFSSAGS
310 320 330 340 350
ELDVMAPGVS IQSTLPGGTY GAYNGTSMAT PHVAGAAALI LSKHPTWTNA
360 370 380
QVRDRLESTA TYLGNSFYYG KGLINVQAAA Q
Length:381
Mass (Da):39,479
Last modified:June 16, 2009 - v3
Checksum:i5C8A4B0E42FCE83D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27A → V in CAA74536 (PubMed:9579061).Curated1
Sequence conflicti191A → S in AAA22742 (PubMed:6427178).Curated1
Sequence conflicti191A → S in CAA74536 (PubMed:9579061).Curated1
Sequence conflicti191A → S (PubMed:7589571).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01988 Genomic DNA Translation: AAA22742.1
Y14083 Genomic DNA Translation: CAA74536.1
AL009126 Genomic DNA Translation: CAB12870.2
K01443 Genomic DNA Translation: AAA22814.1
M16639 Genomic DNA Translation: AAA22744.1
M31060 Genomic DNA Translation: AAA22246.1
M19125 Genomic DNA Translation: AAA22245.1
PIRiA00972 SUBSI
RefSeqiNP_388911.2, NC_000964.3
WP_003233171.1, NZ_JNCM01000035.1
WP_009966941.1, NZ_CM000487.1

Genome annotation databases

EnsemblBacteriaiCAB12870; CAB12870; BSU10300
GeneIDi939313
KEGGibsu:BSU10300
PATRICifig|224308.179.peg.1106

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01988 Genomic DNA Translation: AAA22742.1
Y14083 Genomic DNA Translation: CAA74536.1
AL009126 Genomic DNA Translation: CAB12870.2
K01443 Genomic DNA Translation: AAA22814.1
M16639 Genomic DNA Translation: AAA22744.1
M31060 Genomic DNA Translation: AAA22246.1
M19125 Genomic DNA Translation: AAA22245.1
PIRiA00972 SUBSI
RefSeqiNP_388911.2, NC_000964.3
WP_003233171.1, NZ_JNCM01000035.1
WP_009966941.1, NZ_CM000487.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SCJX-ray2.00A107-381[»]
B36-106[»]
3WHIX-ray2.40A/B30-381[»]
ProteinModelPortaliP04189
SMRiP04189
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005716

Protein family/group databases

MEROPSiS08.036

Proteomic databases

PaxDbiP04189
PRIDEiP04189

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12870; CAB12870; BSU10300
GeneIDi939313
KEGGibsu:BSU10300
PATRICifig|224308.179.peg.1106

Phylogenomic databases

eggNOGiENOG4105RX7 Bacteria
COG1404 LUCA
HOGENOMiHOG000199176
InParanoidiP04189
KOiK01342
OMAiSGQYDWI
PhylomeDBiP04189

Enzyme and pathway databases

BioCyciBSUB:BSU10300-MONOMER
SABIO-RKiP04189

Miscellaneous databases

EvolutionaryTraceiP04189

Family and domain databases

CDDicd07477 Peptidases_S8_Subtilisin_subse, 1 hit
Gene3Di3.30.70.80, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR010259 S8pro/Inhibitor_I9
IPR037045 S8pro/Inhibitor_I9_sf
IPR034202 Subtilisin_Carlsberg-like
PfamiView protein in Pfam
PF05922 Inhibitor_I9, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSUBT_BACSU
AccessioniPrimary (citable) accession number: P04189
Secondary accession number(s): O07613, P70989
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 157 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
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Main funding by: National Institutes of Health

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