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Protein

Granzyme B(G,H)

Gene

Gzmb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (By similarity).By similarity

Catalytic activityi

Preferential cleavage: -Asp-|-Xaa- >> -Asn-|-Xaa- > -Met-|-Xaa-, -Ser-|-Xaa-.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei64Charge relay systemBy similarity1
Active sitei108Charge relay systemBy similarity1
Active sitei203Charge relay systemBy similarity1
Sitei228Mediates preference for Asp-containing substratesBy similarity1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processApoptosis, Cytolysis

Enzyme and pathway databases

ReactomeiR-MMU-75108 Activation, myristolyation of BID and translocation to mitochondria

Protein family/group databases

MEROPSiS01.136

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme B(G,H) (EC:3.4.21.79)
Alternative name(s):
CTLA-1
Cytotoxic cell protease 1
Short name:
CCP1
Fragmentin-2
Gene namesi
Name:Gzmb
Synonyms:Ctla-1, Ctla1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:109267 Gzmb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
PropeptideiPRO_000002740119 – 20Activation peptide1 Publication2
ChainiPRO_000002740221 – 247Granzyme B(G,H)Add BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
Glycosylationi71N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi142 ↔ 209PROSITE-ProRule annotation
Disulfide bondi173 ↔ 188PROSITE-ProRule annotation
Glycosylationi182N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP04187
PaxDbiP04187
PeptideAtlasiP04187
PRIDEiP04187

PTM databases

iPTMnetiP04187
PhosphoSitePlusiP04187

Expressioni

Gene expression databases

BgeeiENSMUSG00000015437 Expressed in 54 organ(s), highest expression level in decidua
CleanExiMM_GZMB
ExpressionAtlasiP04187 baseline and differential
GenevisibleiP04187 MM

Interactioni

Protein-protein interaction databases

BioGridi200135, 6 interactors
IntActiP04187, 1 interactor
MINTiP04187
STRINGi10090.ENSMUSP00000015581

Structurei

3D structure databases

ProteinModelPortaliP04187
SMRiP04187
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 245Peptidase S1PROSITE-ProRule annotationAdd BLAST225

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118895
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP04187
KOiK01353
OMAiYDSTVEL
OrthoDBiEOG091G0G5F
PhylomeDBiP04187
TreeFamiTF333630

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR037553 Granzyme_B
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PANTHERiPTHR24271:SF41 PTHR24271:SF41, 1 hit
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04187-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKILLLLLTL SLASRTKAGE IIGGHEVKPH SRPYMALLSI KDQQPEAICG
60 70 80 90 100
GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY
110 120 130 140 150
NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM
160 170 180 190 200
APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR
210 220 230 240
GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS
Length:247
Mass (Da):27,470
Last modified:March 20, 1987 - v1
Checksum:i996BCD199965C6D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04072 mRNA Translation: CAA27715.1
M12302 mRNA Translation: AAA37383.1
M22526 Genomic DNA Translation: AAB61756.1
BC002085 mRNA Translation: AAH02085.1
U05707 Genomic DNA Translation: AAB60470.1
CCDSiCCDS27147.1
PIRiA94288 PRMSCL
RefSeqiNP_038570.1, NM_013542.3
UniGeneiMm.14874

Genome annotation databases

EnsembliENSMUST00000015581; ENSMUSP00000015581; ENSMUSG00000015437
GeneIDi14939
KEGGimmu:14939
UCSCiuc007ubv.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04072 mRNA Translation: CAA27715.1
M12302 mRNA Translation: AAA37383.1
M22526 Genomic DNA Translation: AAB61756.1
BC002085 mRNA Translation: AAH02085.1
U05707 Genomic DNA Translation: AAB60470.1
CCDSiCCDS27147.1
PIRiA94288 PRMSCL
RefSeqiNP_038570.1, NM_013542.3
UniGeneiMm.14874

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CP1model-A21-247[»]
ProteinModelPortaliP04187
SMRiP04187
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200135, 6 interactors
IntActiP04187, 1 interactor
MINTiP04187
STRINGi10090.ENSMUSP00000015581

Protein family/group databases

MEROPSiS01.136

PTM databases

iPTMnetiP04187
PhosphoSitePlusiP04187

Proteomic databases

EPDiP04187
PaxDbiP04187
PeptideAtlasiP04187
PRIDEiP04187

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015581; ENSMUSP00000015581; ENSMUSG00000015437
GeneIDi14939
KEGGimmu:14939
UCSCiuc007ubv.1 mouse

Organism-specific databases

CTDi3002
MGIiMGI:109267 Gzmb

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118895
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP04187
KOiK01353
OMAiYDSTVEL
OrthoDBiEOG091G0G5F
PhylomeDBiP04187
TreeFamiTF333630

Enzyme and pathway databases

ReactomeiR-MMU-75108 Activation, myristolyation of BID and translocation to mitochondria

Miscellaneous databases

PROiPR:P04187
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015437 Expressed in 54 organ(s), highest expression level in decidua
CleanExiMM_GZMB
ExpressionAtlasiP04187 baseline and differential
GenevisibleiP04187 MM

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR037553 Granzyme_B
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PANTHERiPTHR24271:SF41 PTHR24271:SF41, 1 hit
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGRAB_MOUSE
AccessioniPrimary (citable) accession number: P04187
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: November 7, 2018
This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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