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Protein

Complement factor B

Gene

Cfb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.

Catalytic activityi

Cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to yield C5a and C5b.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei523Charge relay system1
Active sitei573Charge relay system1
Active sitei696Charge relay system1

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: MGI
  • complement activation Source: MGI
  • complement activation, alternative pathway Source: MGI
  • response to thyroid hormone Source: MGI

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processComplement alternate pathway, Immunity, Innate immunity

Enzyme and pathway databases

BRENDAi3.4.21.47 3474
ReactomeiR-MMU-173736 Alternative complement activation
R-MMU-174577 Activation of C3 and C5
R-MMU-977606 Regulation of Complement cascade

Protein family/group databases

MEROPSiS01.196

Names & Taxonomyi

Protein namesi
Recommended name:
Complement factor B (EC:3.4.21.47)
Alternative name(s):
C3/C5 convertase
Cleaved into the following 2 chains:
Gene namesi
Name:Cfb
Synonyms:Bf, H2-Bf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:105975 Cfb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000002754823 – 761Complement factor BAdd BLAST739
ChainiPRO_000002754923 – 256Complement factor B Ba fragmentAdd BLAST234
ChainiPRO_0000027550257 – 761Complement factor B Bb fragmentAdd BLAST505

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi34 ↔ 73By similarity
Disulfide bondi59 ↔ 95By similarity
Disulfide bondi100 ↔ 142By similarity
Glycosylationi119N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi128 ↔ 155By similarity
Glycosylationi139N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi162 ↔ 202By similarity
Disulfide bondi188 ↔ 215By similarity
Glycosylationi282N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi375N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi508 ↔ 524By similarity
Disulfide bondi692 ↔ 722By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP04186
PeptideAtlasiP04186
PRIDEiP04186

PTM databases

iPTMnetiP04186
PhosphoSitePlusiP04186
SwissPalmiP04186

Expressioni

Gene expression databases

BgeeiENSMUSG00000090231 Expressed in 27 organ(s), highest expression level in liver
CleanExiMM_CFB
ExpressionAtlasiP04186 baseline and differential
GenevisibleiP04186 MM

Interactioni

Protein-protein interaction databases

IntActiP04186, 1 interactor
MINTiP04186
STRINGi10090.ENSMUSP00000025229

Structurei

3D structure databases

ProteinModelPortaliP04186
SMRiP04186
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 97Sushi 1PROSITE-ProRule annotationAdd BLAST66
Domaini98 – 157Sushi 2PROSITE-ProRule annotationAdd BLAST60
Domaini160 – 217Sushi 3PROSITE-ProRule annotationAdd BLAST58
Domaini267 – 466VWFAPROSITE-ProRule annotationAdd BLAST200
Domaini474 – 754Peptidase S1PROSITE-ProRule annotationAdd BLAST281

Domaini

The unliganded VWA domain has an inactive 'locked' conformation whereby the scissile Arg-256|Lys-257 bond is protected from proteolytic activation.By similarity

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00530000063826
HOGENOMiHOG000038034
HOVERGENiHBG002567
InParanoidiP04186
OMAiQKGHENC
PhylomeDBiP04186

Family and domain databases

CDDicd00033 CCP, 3 hits
cd00190 Tryp_SPc, 1 hit
Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR011360 Compl_C2_B
IPR028341 Complement_B
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PANTHERiPTHR19325:SF394 PTHR19325:SF394, 1 hit
PfamiView protein in Pfam
PF00084 Sushi, 2 hits
PF00089 Trypsin, 1 hit
PF00092 VWA, 1 hit
PIRSFiPIRSF001154 Compl_C2_B, 1 hit
PIRSF500181 Complement_B, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00032 CCP, 3 hits
SM00020 Tryp_SPc, 1 hit
SM00327 VWA, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF53300 SSF53300, 1 hit
SSF57535 SSF57535, 3 hits
PROSITEiView protein in PROSITE
PS50923 SUSHI, 3 hits
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
PS50234 VWFA, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

P04186-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MESPQLCLVL LVLGFSSGGV SATPVLEARP QVSCSLEGVE IKGGSFQLLQ
60 70 80 90 100
GGQALEYLCP SGFYPYPVQT RTCRSTGSWS DLQTRDQKIV QKAECRAIRC
110 120 130 140 150
PRPQDFENGE FWPRSPFYNL SDQISFQCYD GYVLRGSANR TCQENGRWDG
160 170 180 190 200
QTAICDDGAG YCPNPGIPIG TRKVGSQYRL EDIVTYHCSR GLVLRGSQKR
210 220 230 240 250
KCQEGGSWSG TEPSCQDSFM YDSPQEVAEA FLSSLTETIE GADAEDGHSP
260 270 280 290 300
GEQQKRKIVL DPSGSMNIYL VLDGSDSIGS SNFTGAKRCL TNLIEKVASY
310 320 330 340 350
GVRPRYGLLT YATVPKVLVR VSDERSSDAD WVTEKLNQIS YEDHKLKSGT
360 370 380 390 400
NTKRALQAVY SMMSWAGDAP PEGWNRTRHV IIIMTDGLHN MGGNPVTVIQ
410 420 430 440 450
DIRALLDIGR DPKNPREDYL DVYVFGVGPL VDSVNINALA SKKDNEHHVF
460 470 480 490 500
KVKDMEDLEN VFYQMIDETK SLSLCGMVWE HKKGNDYHKQ PWQAKISVTR
510 520 530 540 550
PLKGHETCMG AVVSEYFVLT AAHCFMVDDQ KHSIKVSVGG QRRDLEIEEV
560 570 580 590 600
LFHPKYNING KKAEGIPEFY DYDVALVKLK NKLKYGQTLR PICLPCTEGT
610 620 630 640 650
TRALRLPQTA TCKQHKEQLL PVKDVKALFV SEQGKSLTRK EVYIKNGDKK
660 670 680 690 700
ASCERDATKA QGYEKVKDAS EVVTPRFLCT GGVDPYADPN TCKGDSGGPL
710 720 730 740 750
IVHKRSRFIQ VGVISWGVVD VCRDQRRQQL VPSYARDFHI NLFQVLPWLK
760
DKLKDEDLGF L
Length:761
Mass (Da):85,004
Last modified:August 1, 1991 - v2
Checksum:i9E9015448A61020E
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B8JJM5B8JJM5_MOUSE
Complement factor B
Cfb
763Annotation score:
B8JJM6B8JJM6_MOUSE
Complement factor B
Cfb
713Annotation score:
F6XQ00F6XQ00_MOUSE
Complement factor B
Cfb
711Annotation score:
B8JJM3B8JJM3_MOUSE
Complement factor B
Cfb
234Annotation score:
F6W2T4F6W2T4_MOUSE
Complement factor B
Cfb
259Annotation score:
H3BK95H3BK95_MOUSE
Complement factor B
Cfb
296Annotation score:
F6WZA2F6WZA2_MOUSE
Complement factor B
Cfb
81Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti730L → P in AAA39549 (PubMed:6689022).Curated1
Sequence conflicti745V → E in AAA39549 (PubMed:6689022).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60646
, M60629, M60630, M60631, M60632, M60633, M60634, M60635, M60636, M60637, M60638, M60639, M60640, M60641, M60642, M60643, M60644, M60645 Genomic DNA Translation: AAA37379.1
AF049850 Genomic DNA Translation: AAC05283.1
AF109906 Genomic DNA Translation: AAC84160.1
BC005451 mRNA Translation: AAH05451.1
K01496 mRNA Translation: AAA39549.1
K01497 mRNA Translation: AAA39550.1
K01498 mRNA Translation: AAA39551.1
M57890 mRNA Translation: AAA63293.1
PIRiA38875 BBMS
UniGeneiMm.653

Genome annotation databases

EnsembliENSMUST00000128767; ENSMUSP00000119977; ENSMUSG00000090231

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60646
, M60629, M60630, M60631, M60632, M60633, M60634, M60635, M60636, M60637, M60638, M60639, M60640, M60641, M60642, M60643, M60644, M60645 Genomic DNA Translation: AAA37379.1
AF049850 Genomic DNA Translation: AAC05283.1
AF109906 Genomic DNA Translation: AAC84160.1
BC005451 mRNA Translation: AAH05451.1
K01496 mRNA Translation: AAA39549.1
K01497 mRNA Translation: AAA39550.1
K01498 mRNA Translation: AAA39551.1
M57890 mRNA Translation: AAA63293.1
PIRiA38875 BBMS
UniGeneiMm.653

3D structure databases

ProteinModelPortaliP04186
SMRiP04186
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04186, 1 interactor
MINTiP04186
STRINGi10090.ENSMUSP00000025229

Protein family/group databases

MEROPSiS01.196

PTM databases

iPTMnetiP04186
PhosphoSitePlusiP04186
SwissPalmiP04186

Proteomic databases

PaxDbiP04186
PeptideAtlasiP04186
PRIDEiP04186

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000128767; ENSMUSP00000119977; ENSMUSG00000090231

Organism-specific databases

MGIiMGI:105975 Cfb

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00530000063826
HOGENOMiHOG000038034
HOVERGENiHBG002567
InParanoidiP04186
OMAiQKGHENC
PhylomeDBiP04186

Enzyme and pathway databases

BRENDAi3.4.21.47 3474
ReactomeiR-MMU-173736 Alternative complement activation
R-MMU-174577 Activation of C3 and C5
R-MMU-977606 Regulation of Complement cascade

Miscellaneous databases

ChiTaRSiCfb mouse
PROiPR:P04186
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000090231 Expressed in 27 organ(s), highest expression level in liver
CleanExiMM_CFB
ExpressionAtlasiP04186 baseline and differential
GenevisibleiP04186 MM

Family and domain databases

CDDicd00033 CCP, 3 hits
cd00190 Tryp_SPc, 1 hit
Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR011360 Compl_C2_B
IPR028341 Complement_B
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PANTHERiPTHR19325:SF394 PTHR19325:SF394, 1 hit
PfamiView protein in Pfam
PF00084 Sushi, 2 hits
PF00089 Trypsin, 1 hit
PF00092 VWA, 1 hit
PIRSFiPIRSF001154 Compl_C2_B, 1 hit
PIRSF500181 Complement_B, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00032 CCP, 3 hits
SM00020 Tryp_SPc, 1 hit
SM00327 VWA, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF53300 SSF53300, 1 hit
SSF57535 SSF57535, 3 hits
PROSITEiView protein in PROSITE
PS50923 SUSHI, 3 hits
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
PS50234 VWFA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCFAB_MOUSE
AccessioniPrimary (citable) accession number: P04186
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: August 1, 1991
Last modified: November 7, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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