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Protein

Urokinase-type plasminogen activator

Gene

PLAU

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei235Charge relay system1
Active sitei286Charge relay system1
Active sitei387Charge relay system1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processPlasminogen activation

Protein family/group databases

MEROPSiS01.231

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 3 chains:
Gene namesi
Name:PLAU
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075030

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
ChainiPRO_000002833021 – 442Urokinase-type plasminogen activatorAdd BLAST422
ChainiPRO_000002833121 – 188Urokinase-type plasminogen activator long chain ABy similarityAdd BLAST168
ChainiPRO_0000285900167 – 188Urokinase-type plasminogen activator short chain ABy similarityAdd BLAST22
ChainiPRO_0000028332190 – 442Urokinase-type plasminogen activator chain BBy similarityAdd BLAST253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 41By similarity
Disulfide bondi35 ↔ 53By similarity
Disulfide bondi55 ↔ 64By similarity
Disulfide bondi72 ↔ 153By similarity
Disulfide bondi93 ↔ 135By similarity
Disulfide bondi124 ↔ 148By similarity
Glycosylationi152N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi179 ↔ 310Interchain (between A and B chains)PROSITE-ProRule annotation
Disulfide bondi220 ↔ 236By similarity
Disulfide bondi228 ↔ 299By similarity
Disulfide bondi324 ↔ 393By similarity
Disulfide bondi356 ↔ 372By similarity
Disulfide bondi383 ↔ 411By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP04185
PRIDEiP04185

Interactioni

Subunit structurei

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR (By similarity).By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000010995

Structurei

3D structure databases

ProteinModelPortaliP04185
SMRiP04185
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 65EGF-likePROSITE-ProRule annotationAdd BLAST37
Domaini72 – 153KringlePROSITE-ProRule annotationAdd BLAST82
Domaini190 – 435Peptidase S1PROSITE-ProRule annotationAdd BLAST246

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 59Binds urokinase plasminogen activator surface receptorBy similarityAdd BLAST24
Regioni154 – 189Connecting peptideAdd BLAST36

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiENOG410IGFI Eukaryota
COG5640 LUCA
HOGENOMiHOG000237314
HOVERGENiHBG008633
InParanoidiP04185
KOiK01348

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
Gene3Di2.40.20.10, 1 hit
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
IPR034814 Urokinase
PANTHERiPTHR24264:SF38 PTHR24264:SF38, 1 hit
PfamiView protein in Pfam
PF00051 Kringle, 1 hit
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00130 KR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit
PS00021 KRINGLE_1, 1 hit
PS50070 KRINGLE_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04185-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLRACLSL CVLVVSDSKG SHELHQESGA SNCGCLNGGK CVSYKYFSNI
60 70 80 90 100
QRCSCPKKFQ GEHCEIDTSQ TCFEGNGHSY RGKANTNTGG RPCLPWNSAT
110 120 130 140 150
VLLNTYHAHR PDALQLGLGK HNYCRNPDNQ RRPWCYVQVG LKQLVQECMV
160 170 180 190 200
PNCSGGESHR PAYDGKNPFS TPEKVEFQCG QKALRPRFKI VGGKSTTIEN
210 220 230 240 250
QPWFAAIYRR HRGGSVTYVC GGSLISPCWV VSATHCFINY QQKEDYIVYL
260 270 280 290 300
GRQTLHSSTH GEMKFEVEKL ILHEDYSADS LAHHNDIALL KIRTDKGQCA
310 320 330 340 350
QPSRSIQTIC LPPVNGDAHF GASCEIVGFG KEDPSDYLYP EQLKMTVVKL
360 370 380 390 400
VSHRECQQPH YYGSEVTTKM LCAADPQWKT DSCQGDSGGP LVCSTQGRLT
410 420 430 440
LTGIVSWGRE CAMKDKPGVY TRVSRFLTWI HTHVGGENGL AH
Length:442
Mass (Da):49,117
Last modified:August 13, 1987 - v1
Checksum:iEE32FCEF501321EE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti241Q → H in CAA25806 (PubMed:6096832).Curated1
Sequence conflicti242Q → H in CAA26511 (PubMed:6096832).Curated1
Sequence conflicti288A → GS in CAA25806 (PubMed:6096832).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01648 Genomic DNA Translation: CAA25806.1
X02724 mRNA Translation: CAA26511.1
PIRiA00932 UKPG
RefSeqiNP_999110.1, NM_213945.1
UniGeneiSsc.11194

Genome annotation databases

GeneIDi396985
KEGGissc:396985

Similar proteinsi

Entry informationi

Entry nameiUROK_PIG
AccessioniPrimary (citable) accession number: P04185
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: August 13, 1987
Last modified: March 28, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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