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Protein

Thymidine kinase, cytosolic

Gene

Tk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98Proton acceptorSequence analysis1
Binding sitei128Substrate; via amide nitrogenBy similarity1
Metal bindingi153ZincBy similarity1
Metal bindingi156ZincBy similarity1
Binding sitei181SubstrateBy similarity1
Metal bindingi185ZincBy similarity1
Metal bindingi188ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33ATPCurated8
Nucleotide bindingi58 – 60ATPBy similarity3
Nucleotide bindingi97 – 100ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • DNA biosynthetic process Source: UniProtKB-KW
  • DNA metabolic process Source: GO_Central
  • protein homotetramerization Source: MGI
  • thymidine metabolic process Source: MGI

Keywordsi

Molecular functionKinase, Transferase
Biological processDNA synthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.21 3474
ReactomeiR-MMU-73614 Pyrimidine salvage
SABIO-RKiP04184

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:Tk1
Synonyms:Tk-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98763 Tk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2618

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001749492 – 233Thymidine kinase, cytosolicAdd BLAST232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Ser-13 in mitosis.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP04184
MaxQBiP04184
PaxDbiP04184
PeptideAtlasiP04184
PRIDEiP04184

PTM databases

iPTMnetiP04184
PhosphoSitePlusiP04184

Expressioni

Gene expression databases

BgeeiENSMUSG00000025574 Expressed in 209 organ(s), highest expression level in fetal liver hematopoietic progenitor cell
CleanExiMM_TK1
GenevisibleiP04184 MM

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204212, 1 interactor
STRINGi10090.ENSMUSP00000026661

Chemistry databases

BindingDBiP04184

Structurei

3D structure databases

ProteinModelPortaliP04184
SMRiP04184
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 176Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

eggNOGiKOG3125 Eukaryota
COG1435 LUCA
GeneTreeiENSGT00390000011309
HOGENOMiHOG000076390
HOVERGENiHBG006215
InParanoidiP04184
KOiK00857
OMAiCAGLDQD
OrthoDBiEOG091G0HZR
PhylomeDBiP04184
TreeFamiTF314839

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR001267 Thymidine_kinase
IPR020633 Thymidine_kinase_CS
PANTHERiPTHR11441 PTHR11441, 1 hit
PfamiView protein in Pfam
PF00265 TK, 1 hit
PIRSFiPIRSF035805 TK_cell, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00603 TK_CELLULAR_TYPE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04184-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSYINLPTVL PSSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQYKC
60 70 80 90 100
LVIKYAKDTR YSNSFSTHDR NTMDALPACM LRDVTQESLG VAVIGIDEGQ
110 120 130 140 150
FFPDIVDFCE MMANEGKTVI VAALDGTFQR KAFGSILNLV PLAESVVKLT
160 170 180 190 200
AVCMECFREA AYTKRLGLEK EVEVIGGADK YHSVCRLCYF KKSSAQTAGS
210 220 230
DNKNCLVLGQ PGEALVVRKL FASQQVLQYN SAN
Length:233
Mass (Da):25,776
Last modified:February 26, 2008 - v3
Checksum:i78366623EDED0C6C
GO

Sequence cautioni

The sequence AAA40452 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27P → L in AAH91758 (PubMed:15489334).Curated1
Sequence conflicti88S → L in AAA40451 (PubMed:3018504).Curated1
Sequence conflicti88S → L in AAA40452 (PubMed:3018504).Curated1
Sequence conflicti185C → G in BAC39384 (PubMed:16141072).Curated1
Sequence conflicti198A → V in BAC39384 (PubMed:16141072).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti88S → A1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11945 mRNA Translation: AAA40451.1
M19438 mRNA Translation: AAA40452.1 Different initiation.
M68489 Genomic DNA Translation: AAA40454.1
S57244 mRNA Translation: AAB26001.1
AK085188 mRNA Translation: BAC39384.1
AK087988 mRNA Translation: BAC40077.1
AK139244 mRNA Translation: BAE23931.1
BC012215 mRNA Translation: AAH12215.1
BC091758 mRNA Translation: AAH91758.1
X60980 Genomic DNA Translation: CAA43296.1
M22581 Genomic DNA Translation: AAA40450.1
CCDSiCCDS25692.1
PIRiJC1252 KIMST
RefSeqiNP_001258658.1, NM_001271729.1
NP_033413.2, NM_009387.2
UniGeneiMm.2661
Mm.443968

Genome annotation databases

EnsembliENSMUST00000026661; ENSMUSP00000026661; ENSMUSG00000025574
GeneIDi21877
KEGGimmu:21877
UCSCiuc007mnz.2 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11945 mRNA Translation: AAA40451.1
M19438 mRNA Translation: AAA40452.1 Different initiation.
M68489 Genomic DNA Translation: AAA40454.1
S57244 mRNA Translation: AAB26001.1
AK085188 mRNA Translation: BAC39384.1
AK087988 mRNA Translation: BAC40077.1
AK139244 mRNA Translation: BAE23931.1
BC012215 mRNA Translation: AAH12215.1
BC091758 mRNA Translation: AAH91758.1
X60980 Genomic DNA Translation: CAA43296.1
M22581 Genomic DNA Translation: AAA40450.1
CCDSiCCDS25692.1
PIRiJC1252 KIMST
RefSeqiNP_001258658.1, NM_001271729.1
NP_033413.2, NM_009387.2
UniGeneiMm.2661
Mm.443968

3D structure databases

ProteinModelPortaliP04184
SMRiP04184
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204212, 1 interactor
STRINGi10090.ENSMUSP00000026661

Chemistry databases

BindingDBiP04184
ChEMBLiCHEMBL2618

PTM databases

iPTMnetiP04184
PhosphoSitePlusiP04184

Proteomic databases

EPDiP04184
MaxQBiP04184
PaxDbiP04184
PeptideAtlasiP04184
PRIDEiP04184

Protocols and materials databases

DNASUi21877
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026661; ENSMUSP00000026661; ENSMUSG00000025574
GeneIDi21877
KEGGimmu:21877
UCSCiuc007mnz.2 mouse

Organism-specific databases

CTDi7083
MGIiMGI:98763 Tk1

Phylogenomic databases

eggNOGiKOG3125 Eukaryota
COG1435 LUCA
GeneTreeiENSGT00390000011309
HOGENOMiHOG000076390
HOVERGENiHBG006215
InParanoidiP04184
KOiK00857
OMAiCAGLDQD
OrthoDBiEOG091G0HZR
PhylomeDBiP04184
TreeFamiTF314839

Enzyme and pathway databases

BRENDAi2.7.1.21 3474
ReactomeiR-MMU-73614 Pyrimidine salvage
SABIO-RKiP04184

Miscellaneous databases

PROiPR:P04184
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025574 Expressed in 209 organ(s), highest expression level in fetal liver hematopoietic progenitor cell
CleanExiMM_TK1
GenevisibleiP04184 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR001267 Thymidine_kinase
IPR020633 Thymidine_kinase_CS
PANTHERiPTHR11441 PTHR11441, 1 hit
PfamiView protein in Pfam
PF00265 TK, 1 hit
PIRSFiPIRSF035805 TK_cell, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00603 TK_CELLULAR_TYPE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_MOUSE
AccessioniPrimary (citable) accession number: P04184
Secondary accession number(s): Q58EU0, Q8C3P8, Q921J8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: February 26, 2008
Last modified: September 12, 2018
This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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