UniProtKB - P04173 (LEU3_YEAST)
Protein
3-isopropylmalate dehydrogenase
Gene
LEU2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activityi
- EC:1.1.1.85
Cofactori
: L-leucine biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- 2-isopropylmalate synthase (LEU4), 2-isopropylmalate synthase 2, mitochondrial (LEU9)
- 3-isopropylmalate dehydratase (LEU1)
- 3-isopropylmalate dehydrogenase (LEU2)
- Branched-chain-amino-acid aminotransferase, mitochondrial (BAT1), Branched-chain-amino-acid aminotransferase, cytosolic (BAT2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 97 | SubstrateBy similarity | 1 | |
Binding sitei | 107 | SubstrateBy similarity | 1 | |
Binding sitei | 136 | SubstrateBy similarity | 1 | |
Sitei | 143 | Important for catalysisBy similarity | 1 | |
Sitei | 192 | Important for catalysisBy similarity | 1 | |
Metal bindingi | 225 | Magnesium or manganeseBy similarity | 1 | |
Binding sitei | 225 | SubstrateBy similarity | 1 | |
Metal bindingi | 250 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 254 | Magnesium or manganeseBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 79 – 90 | NADBy similarityAdd BLAST | 12 | |
Nucleotide bindingi | 289 – 300 | NADBy similarityAdd BLAST | 12 |
GO - Molecular functioni
- 3-isopropylmalate dehydrogenase activity Source: SGD
- magnesium ion binding Source: InterPro
- NAD binding Source: InterPro
GO - Biological processi
- glyoxylate cycle Source: SGD
- leucine biosynthetic process Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis |
Ligand | Magnesium, Manganese, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | MetaCyc:YCL018W-MONOMER |
UniPathwayi | UPA00048;UER00072 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-isopropylmalate dehydrogenase (EC:1.1.1.85)Short name: 3-IPM-DH Short name: IMDH Alternative name(s): Beta-IPM dehydrogenase |
Gene namesi | Name:LEU2 Ordered Locus Names:YCL018W ORF Names:YCL18W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YCL018W |
SGDi | S000000523, LEU2 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: SGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083623 | 1 – 364 | 3-isopropylmalate dehydrogenaseAdd BLAST | 364 |
Proteomic databases
PaxDbi | P04173 |
PRIDEi | P04173 |
2D gel databases
SWISS-2DPAGEi | P04173 |
PTM databases
iPTMneti | P04173 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 30966, 20 interactors |
DIPi | DIP-7880N |
IntActi | P04173, 157 interactors |
MINTi | P04173 |
STRINGi | 4932.YCL018W |
Miscellaneous databases
RNActi | P04173, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the isocitrate and isopropylmalate dehydrogenases family.Curated
Phylogenomic databases
eggNOGi | KOG0786, Eukaryota |
GeneTreei | ENSGT00950000182989 |
HOGENOMi | CLU_031953_0_3_1 |
InParanoidi | P04173 |
OMAi | EYDLGAR |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR024084, IsoPropMal-DH-like_dom IPR004429, Isopropylmalate_DH |
PANTHERi | PTHR42979, PTHR42979, 1 hit |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00169, leuB, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
i Sequence
Sequence statusi: Complete.
P04173-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAPKKIVVL PGDHVGQEIT AEAIKVLKAI SDVRSNVKFD FENHLIGGAA
60 70 80 90 100
IDATGVPLPD EALEASKKAD AVLLGAVGGP KWGTGSVRPE QGLLKIRKEL
110 120 130 140 150
QLYANLRPCN FASDSLLDLS PIKPQFAKGT DFVVVRELVG GIYFGKRKED
160 170 180 190 200
DGDGVAWDSE QYTVPEVQRI TRMAAFMALQ HEPPLPIWSL DKANVLASSR
210 220 230 240 250
LWRKTVEETI KNEFPTLKVQ HQLIDSAAMI LVKNPTHLNG IIITSNMFGD
260 270 280 290 300
IISDEASVIP GSLGLLPSAS LASLPDKNTA FGLYEPCHGS APDLPKNKVN
310 320 330 340 350
PIATILSAAM MLKLSLNLPE EGKAIEDAVK KVLDAGIRTG DLGGSNSTTE
360
VGDAVAEEVK KILA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 69 | A → V (PubMed:3010239).Curated | 1 | |
Sequence conflicti | 69 | A → V (PubMed:6330094).Curated | 1 | |
Sequence conflicti | 69 | A → V (Ref. 3) Curated | 1 | |
Sequence conflicti | 300 | N → D (PubMed:6330094).Curated | 1 | |
Sequence conflicti | 300 | N → D (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03840 Genomic DNA Translation: CAA27459.1 X59720 Genomic DNA Translation: CAA42366.2 M12909 Genomic DNA Translation: AAA66917.1 BK006937 Genomic DNA Translation: DAA07465.1 |
PIRi | S19344, DEBYI |
RefSeqi | NP_009911.2, NM_001178665.1 |
Genome annotation databases
EnsemblFungii | YCL018W_mRNA; YCL018W; YCL018W |
GeneIDi | 850342 |
KEGGi | sce:YCL018W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03840 Genomic DNA Translation: CAA27459.1 X59720 Genomic DNA Translation: CAA42366.2 M12909 Genomic DNA Translation: AAA66917.1 BK006937 Genomic DNA Translation: DAA07465.1 |
PIRi | S19344, DEBYI |
RefSeqi | NP_009911.2, NM_001178665.1 |
3D structure databases
SMRi | P04173 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 30966, 20 interactors |
DIPi | DIP-7880N |
IntActi | P04173, 157 interactors |
MINTi | P04173 |
STRINGi | 4932.YCL018W |
PTM databases
iPTMneti | P04173 |
2D gel databases
SWISS-2DPAGEi | P04173 |
Proteomic databases
PaxDbi | P04173 |
PRIDEi | P04173 |
Genome annotation databases
EnsemblFungii | YCL018W_mRNA; YCL018W; YCL018W |
GeneIDi | 850342 |
KEGGi | sce:YCL018W |
Organism-specific databases
EuPathDBi | FungiDB:YCL018W |
SGDi | S000000523, LEU2 |
Phylogenomic databases
eggNOGi | KOG0786, Eukaryota |
GeneTreei | ENSGT00950000182989 |
HOGENOMi | CLU_031953_0_3_1 |
InParanoidi | P04173 |
OMAi | EYDLGAR |
Enzyme and pathway databases
UniPathwayi | UPA00048;UER00072 |
BioCyci | MetaCyc:YCL018W-MONOMER |
Miscellaneous databases
PHI-basei | PHI:504 |
PROi | PR:P04173 |
RNActi | P04173, protein |
Family and domain databases
InterProi | View protein in InterPro IPR019818, IsoCit/isopropylmalate_DH_CS IPR024084, IsoPropMal-DH-like_dom IPR004429, Isopropylmalate_DH |
PANTHERi | PTHR42979, PTHR42979, 1 hit |
Pfami | View protein in Pfam PF00180, Iso_dh, 1 hit |
SMARTi | View protein in SMART SM01329, Iso_dh, 1 hit |
TIGRFAMsi | TIGR00169, leuB, 1 hit |
PROSITEi | View protein in PROSITE PS00470, IDH_IMDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LEU3_YEAST | |
Accessioni | P04173Primary (citable) accession number: P04173 Secondary accession number(s): D6VQZ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 1987 |
Last sequence update: | March 15, 2004 | |
Last modified: | December 2, 2020 | |
This is version 181 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome III
Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names