UniProtKB - P04167 (CP2B2_RAT)
Cytochrome P450 2B2
Cyp2b2
Functioni
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activityi
- an organic molecule + O2 + reduced [NADPH—hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]EC:1.14.14.1
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 436 | Iron (heme axial ligand) | 1 |
GO - Molecular functioni
- arachidonic acid epoxygenase activity Source: GO_Central
- aromatase activity Source: UniProtKB-EC
- heme binding Source: GO_Central
- iron ion binding Source: InterPro
- oxidoreductase activity Source: RGD
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
- steroid hydroxylase activity Source: RGD
GO - Biological processi
- aging Source: RGD
- epoxygenase P450 pathway Source: GO_Central
- nicotine metabolic process Source: RGD
- organic acid metabolic process Source: GO_Central
- response to calcium ion Source: RGD
- response to metal ion Source: RGD
- response to organic cyclic compound Source: RGD
- xenobiotic metabolic process Source: RGD
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 2B2 (EC:1.14.14.1)Alternative name(s): CYPIIB2 Cytochrome P450 PB4 Cytochrome P450E |
Gene namesi | Name:Cyp2b2 Synonyms:Cyp2b-2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2467, Cyp2b2 |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: RGD
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051679 | 1 – 491 | Cytochrome P450 2B2Add BLAST | 491 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 128 | Phosphoserine; by PKA1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P04167 |
PeptideAtlasi | P04167 |
PTM databases
iPTMneti | P04167 |
Expressioni
Inductioni
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
InParanoidi | P04167 |
OrthoDBi | 702827at2759 |
PhylomeDBi | P04167 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR008068, Cyt_P450_E_grp-I_CYP2B-like IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR01685, EP450ICYP2B PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEPSILLLLA LLVGFLLLLV RGHPKSRGNF PPGPRPLPLL GNLLQLDRGG
60 70 80 90 100
LLNSFMQLRE KYGDVFTVHL GPRPVVMLCG TDTIKEALVG QAEDFSGRGT
110 120 130 140 150
IAVIEPIFKE YGVIFANGER WKALRRFSLA TMRDFGMGKR SVEERIQEEA
160 170 180 190 200
QCLVEELRKS QGAPLDPTFL FQCITANIIC SIVFGERFDY TDRQFLRLLE
210 220 230 240 250
LFYRTFSLLS SFSSQVFEFF SGFLKYFPGA HRQISKNLQE ILDYIGHIVE
260 270 280 290 300
KHRATLDPSA PRDFIDTYLL RMEKEKSNHH TEFHHENLMI SLLSLFFAGT
310 320 330 340 350
ETGSTTLRYG FLLMLKYPHV TEKVQKEIDQ VIGSHRPPSL DDRTKMPYTD
360 370 380 390 400
AVIHEIQRFA DLAPIGLPHR VTKDTMFRGY LLPKNTEVYP ILSSALHDPQ
410 420 430 440 450
YFDHPDTFNP EHFLDADGTL KKSEAFMPFS TGKRICLGEG IARNELFLFF
460 470 480 490
TTILQNFSVS SHLAPKDIDL TPKESGIAKI PPTYQICFSA R
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A8I6GGX5 | A0A8I6GGX5_RAT | Cytochrome P450 2B2 | Cyp2b2 | 499 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 114 | I → F in AAA41004 (PubMed:2323573).Curated | 1 | |
Sequence conflicti | 292 | L → P AA sequence (PubMed:3877725).Curated | 1 | |
Sequence conflicti | 321 | T → A AA sequence (PubMed:3877725).Curated | 1 | |
Sequence conflicti | 321 | T → A in AAA41029 (PubMed:6688421).Curated | 1 | |
Sequence conflicti | 322 | E → V in AAA41056 (PubMed:6306654).Curated | 1 | |
Sequence conflicti | 359 – 360 | FA → AS no nucleotide entry (PubMed:2539047).Curated | 2 | |
Sequence conflicti | 438 | G → D in AAA41029 (PubMed:6688421).Curated | 1 | |
Sequence conflicti | 444 | N → K in AAA41026 (PubMed:6689485).Curated | 1 | |
Sequence conflicti | 473 | K → M in AAA41056 (PubMed:6306654).Curated | 1 | |
Sequence conflicti | 476 | G → D AA sequence (PubMed:3877725).Curated | 1 | |
Sequence conflicti | 476 | G → D in AAA41029 (PubMed:6688421).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011939 | 274 | K → KVSPAWMRE in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J00728 , J00720, J00721, J00722, J00723, J00724, J00725, J00726 Genomic DNA Translation: AAA41056.1 M34452 mRNA Translation: AAA41004.1 K01721 mRNA Translation: AAA41026.1 K00996 mRNA Translation: AAA41029.1 M13234 Genomic DNA Translation: AAA41057.1 K01626 mRNA Translation: AAA41037.1 D00250 Genomic DNA Translation: BAA00181.1 |
PIRi | A21162, O4RTP2 A60822 |
RefSeqi | NP_001185605.1, NM_001198676.1 |
Genome annotation databases
GeneIDi | 361523 |
KEGGi | rno:361523 |
UCSCi | RGD:2467, rat [P04167-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J00728 , J00720, J00721, J00722, J00723, J00724, J00725, J00726 Genomic DNA Translation: AAA41056.1 M34452 mRNA Translation: AAA41004.1 K01721 mRNA Translation: AAA41026.1 K00996 mRNA Translation: AAA41029.1 M13234 Genomic DNA Translation: AAA41057.1 K01626 mRNA Translation: AAA41037.1 D00250 Genomic DNA Translation: BAA00181.1 |
PIRi | A21162, O4RTP2 A60822 |
RefSeqi | NP_001185605.1, NM_001198676.1 |
3D structure databases
AlphaFoldDBi | P04167 |
SMRi | P04167 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000045196 |
Chemistry databases
DrugBanki | DB08834, Tauroursodeoxycholic acid |
PTM databases
iPTMneti | P04167 |
Proteomic databases
PaxDbi | P04167 |
PeptideAtlasi | P04167 |
Genome annotation databases
GeneIDi | 361523 |
KEGGi | rno:361523 |
UCSCi | RGD:2467, rat [P04167-1] |
Organism-specific databases
CTDi | 361523 |
RGDi | 2467, Cyp2b2 |
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
InParanoidi | P04167 |
OrthoDBi | 702827at2759 |
PhylomeDBi | P04167 |
Miscellaneous databases
PROi | PR:P04167 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR008068, Cyt_P450_E_grp-I_CYP2B-like IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR01685, EP450ICYP2B PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP2B2_RAT | |
Accessioni | P04167Primary (citable) accession number: P04167 Secondary accession number(s): Q64579, Q64582 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 1987 |
Last sequence update: | July 15, 1998 | |
Last modified: | May 25, 2022 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families