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Protein

Protein UmuC

Gene

umuC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in UV protection and mutation. Poorly processive, error-prone DNA polymerase involved in translesion repair (PubMed:10801133). Essential for induced (or SOS) mutagenesis. Able to replicate DNA across DNA lesions (thymine photodimers and abasic sites, translesion synthesis) in the presence of activated RecA; efficiency is maximal in the presence of the beta sliding-clamp and clamp-loading complex of DNA polymerase III plus single-stranded binding protein (SSB) (PubMed:10801133). RecA and to a lesser extent the beta clamp-complex may target Pol V to replication complexes stalled at DNA template lesions (PubMed:10801133).1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, SOS mutagenesis, SOS response

Enzyme and pathway databases

BioCyciEcoCyc:EG11056-MONOMER
MetaCyc:EG11056-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein UmuC
Alternative name(s):
DNA polymerase V
Short name:
Pol V
Gene namesi
Name:umuC
Ordered Locus Names:b1184, JW1173
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11056 umuC

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi75E → K in umuC36; non-mutable. 1 Publication1
Mutagenesisi101D → N in umuC104; non-mutable. 1 Publication1
Mutagenesisi290T → K in umuC25; non-mutable. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739821 – 422Protein UmuCAdd BLAST422

Proteomic databases

PaxDbiP04152
PRIDEiP04152

Expressioni

Inductioni

Repressed by LexA, induced by DNA damage (PubMed:10760155). Induced 1.5-fold by hydroxyurea (PubMed:20005847).2 Publications

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
umuDP0AG114EBI-1119849,EBI-1122622

Protein-protein interaction databases

BioGridi4261816, 142 interactors
DIPiDIP-11090N
IntActiP04152, 11 interactors
STRINGi316385.ECDH10B_1237

Structurei

3D structure databases

ProteinModelPortaliP04152
SMRiP04152
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 188UmuCPROSITE-ProRule annotationAdd BLAST187

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated

Phylogenomic databases

eggNOGiENOG4105CQ3 Bacteria
COG0389 LUCA
HOGENOMiHOG000252172
InParanoidiP04152
KOiK03502
PhylomeDBiP04152

Family and domain databases

Gene3Di3.30.1490.100, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR025188 DUF4113
IPR024728 PolY_HhH_motif
IPR001126 UmuC
PfamiView protein in Pfam
PF13438 DUF4113, 1 hit
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PF11798 IMS_HHH, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequencei

Sequence statusi: Complete.

P04152-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFALCDVNAF YASCETVFRP DLWGKPVVVL SNNDGCVIAR NAEAKALGVK
60 70 80 90 100
MGDPWFKQKD LFRRCGVVCF SSNYELYADM SNRVMSTLEE LSPRVEIYSI
110 120 130 140 150
DEAFCDLTGV RNCRDLTDFG REIRATVLQR THLTVGVGIA QTKTLAKLAN
160 170 180 190 200
HAAKKWQRQT GGVVDLSNLE RQRKLMSALP VDDVWGIGRR ISKKLDAMGI
210 220 230 240 250
KTVLDLADTD IRFIRKHFNV VLERTVRELR GEPCLQLEEF APTKQEIICS
260 270 280 290 300
RSFGERITDY PSMRQAICSY AARAAEKLRS EHQYCRFIST FIKTSPFALN
310 320 330 340 350
EPYYGNSASV KLLTPTQDSR DIINAATRSL DAIWQAGHRY QKAGVMLGDF
360 370 380 390 400
FSQGVAQLNL FDDNAPRPGS EQLMTVMDTL NAKEGRGTLY FAGQGIQQQW
410 420
QMKRAMLSPR YTTRSSDLLR VK
Length:422
Mass (Da):47,680
Last modified:November 1, 1997 - v2
Checksum:i0F1855D802A3F5F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152A → E in AAA24729 (PubMed:2989817).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10107 Genomic DNA Translation: AAA24729.1
M13387 Genomic DNA Translation: AAA98074.1
U00096 Genomic DNA Translation: AAC74268.1
AP009048 Genomic DNA Translation: BAA36031.1
PIRiE64864 ZWECC
RefSeqiNP_415702.1, NC_000913.3
WP_000457616.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74268; AAC74268; b1184
BAA36031; BAA36031; BAA36031
GeneIDi946359
KEGGiecj:JW1173
eco:b1184
PATRICifig|1411691.4.peg.1103

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10107 Genomic DNA Translation: AAA24729.1
M13387 Genomic DNA Translation: AAA98074.1
U00096 Genomic DNA Translation: AAC74268.1
AP009048 Genomic DNA Translation: BAA36031.1
PIRiE64864 ZWECC
RefSeqiNP_415702.1, NC_000913.3
WP_000457616.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP04152
SMRiP04152
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261816, 142 interactors
DIPiDIP-11090N
IntActiP04152, 11 interactors
STRINGi316385.ECDH10B_1237

Proteomic databases

PaxDbiP04152
PRIDEiP04152

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74268; AAC74268; b1184
BAA36031; BAA36031; BAA36031
GeneIDi946359
KEGGiecj:JW1173
eco:b1184
PATRICifig|1411691.4.peg.1103

Organism-specific databases

EchoBASEiEB1049
EcoGeneiEG11056 umuC

Phylogenomic databases

eggNOGiENOG4105CQ3 Bacteria
COG0389 LUCA
HOGENOMiHOG000252172
InParanoidiP04152
KOiK03502
PhylomeDBiP04152

Enzyme and pathway databases

BioCyciEcoCyc:EG11056-MONOMER
MetaCyc:EG11056-MONOMER

Miscellaneous databases

PROiPR:P04152

Family and domain databases

Gene3Di3.30.1490.100, 1 hit
InterProiView protein in InterPro
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR025188 DUF4113
IPR024728 PolY_HhH_motif
IPR001126 UmuC
PfamiView protein in Pfam
PF13438 DUF4113, 1 hit
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PF11798 IMS_HHH, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUMUC_ECOLI
AccessioniPrimary (citable) accession number: P04152
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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