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Protein

Granulocyte-macrophage colony-stimulating factor

Gene

CSF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Growth factor

Enzyme and pathway databases

ReactomeiR-HSA-392451 G beta:gamma signalling through PI3Kgamma
R-HSA-512988 Interleukin-3, 5 and GM-CSF signaling
R-HSA-5673001 RAF/MAP kinase cascade
R-HSA-6783783 Interleukin-10 signaling
R-HSA-8939246 RUNX1 regulates transcription of genes involved in differentiation of myeloid cells
R-HSA-912526 Interleukin receptor SHC signaling
SignaLinkiP04141
SIGNORiP04141

Names & Taxonomyi

Protein namesi
Recommended name:
Granulocyte-macrophage colony-stimulating factor
Short name:
GM-CSF
Alternative name(s):
Colony-stimulating factor
Short name:
CSF
Molgramostin
Sargramostim
Gene namesi
Name:CSF2
Synonyms:GMCSF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000164400.5
HGNCiHGNC:2434 CSF2
MIMi138960 gene
neXtProtiNX_P04141

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Organism-specific databases

DisGeNETi1437
OpenTargetsiENSG00000164400
PharmGKBiPA26937

Chemistry databases

ChEMBLiCHEMBL3712955
DrugBankiDB05194 KB002

Polymorphism and mutation databases

BioMutaiCSF2
DMDMi117561

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Add BLAST17
ChainiPRO_000000586518 – 144Granulocyte-macrophage colony-stimulating factorAdd BLAST127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi22O-linked (GalNAc...) serine1 Publication1
Glycosylationi24O-linked (GalNAc...) serine1 Publication1
Glycosylationi26O-linked (GalNAc...) serine1 Publication1
Glycosylationi27O-linked (GalNAc...) threonine; partial1 Publication1
Glycosylationi44N-linked (GlcNAc...) asparagine1
Glycosylationi54N-linked (GlcNAc...) asparagine1
Disulfide bondi71 ↔ 113Combined sources2 Publications
Disulfide bondi105 ↔ 138Combined sources2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP04141
PeptideAtlasiP04141
PRIDEiP04141
ProteomicsDBi51656

PTM databases

iPTMnetiP04141
PhosphoSitePlusiP04141

Miscellaneous databases

PMAP-CutDBiP04141

Expressioni

Gene expression databases

BgeeiENSG00000164400
CleanExiHS_CSF2
GenevisibleiP04141 HS

Organism-specific databases

HPAiHPA057404
HPA062006

Interactioni

Subunit structurei

Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • cytokine activity Source: UniProtKB
  • granulocyte macrophage colony-stimulating factor receptor binding Source: ProtInc
  • growth factor activity Source: UniProtKB-KW
  • Ras guanyl-nucleotide exchange factor activity Source: Reactome

Protein-protein interaction databases

BioGridi107824, 11 interactors
CORUMiP04141
DIPiDIP-386N
IntActiP04141, 3 interactors
STRINGi9606.ENSP00000296871

Structurei

Secondary structure

1144
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni25 – 27Combined sources3
Turni29 – 31Combined sources3
Helixi32 – 45Combined sources14
Helixi50 – 53Combined sources4
Beta strandi56 – 60Combined sources5
Beta strandi66 – 68Combined sources3
Helixi72 – 80Combined sources9
Helixi88 – 90Combined sources3
Helixi91 – 104Combined sources14
Beta strandi115 – 119Combined sources5
Helixi120 – 133Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CSGX-ray2.70A/B18-144[»]
2GMFX-ray2.40A/B18-144[»]
4NKQX-ray3.30C18-144[»]
4RS1X-ray2.68A31-142[»]
5C7XX-ray2.95A/B18-144[»]
5D70X-ray2.06A18-144[»]
5D71X-ray2.25A1-127[»]
5D72X-ray2.60A/B1-127[»]
ProteinModelPortaliP04141
SMRiP04141
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04141

Family & Domainsi

Sequence similaritiesi

Belongs to the GM-CSF family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JBQ7 Eukaryota
ENOG411164J LUCA
GeneTreeiENSGT00390000013425
HOGENOMiHOG000037940
HOVERGENiHBG000532
InParanoidiP04141
KOiK05427
OMAiMWLQNLL
OrthoDBiEOG091G0XFL
PhylomeDBiP04141
TreeFamiTF338611

Family and domain databases

CDDicd00040 CSF2, 1 hit
InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR000773 GM_colony-stim-fac
PANTHERiPTHR10059 PTHR10059, 1 hit
PfamiView protein in Pfam
PF01109 GM_CSF, 1 hit
PRINTSiPR00693 GMCSFACTOR
ProDomiView protein in ProDom or Entries sharing at least one domain
PD007349 GM_colony-stim-fac, 1 hit
SMARTiView protein in SMART
SM00040 CSF2, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit
PROSITEiView protein in PROSITE
PS00702 GM_CSF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLQSLLLLG TVACSISAPA RSPSPSTQPW EHVNAIQEAR RLLNLSRDTA
60 70 80 90 100
AEMNETVEVI SEMFDLQEPT CLQTRLELYK QGLRGSLTKL KGPLTMMASH
110 120 130 140
YKQHCPPTPE TSCATQIITF ESFKENLKDF LLVIPFDCWE PVQE
Length:144
Mass (Da):16,295
Last modified:November 1, 1986 - v1
Checksum:i75D1E50506BCA7A8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96M → K in AAM44054 (Ref. 9) Curated1
Sequence conflicti123F → L in AAM44054 (Ref. 9) Curated1

Polymorphismi

Variant Ile-117 may be a risk factor for atopic asthma.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_013089115T → I1 PublicationCorresponds to variant dbSNP:rs2069640Ensembl.1
Natural variantiVAR_001975117I → T3 PublicationsCorresponds to variant dbSNP:rs25882Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13207 Genomic DNA Translation: AAA98768.1
M11734 mRNA Translation: AAA52122.1
M11220 mRNA Translation: AAA52578.1
X03021 Genomic DNA Translation: CAA26822.1
M10663 mRNA Translation: AAA52121.1
AF373868 Genomic DNA Translation: AAK51563.1
AC004511 Genomic DNA Translation: AAC08707.1
BC108724 mRNA Translation: AAI08725.1
BC113924 mRNA Translation: AAI13925.1
BC113999 mRNA Translation: AAI14000.1
AF510855 mRNA Translation: AAM44054.1
CCDSiCCDS4150.1
PIRiC24636 FQHUGM
RefSeqiNP_000749.2, NM_000758.3
UniGeneiHs.1349

Genome annotation databases

EnsembliENST00000296871; ENSP00000296871; ENSG00000164400
GeneIDi1437
KEGGihsa:1437
UCSCiuc003kwf.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCSF2_HUMAN
AccessioniPrimary (citable) accession number: P04141
Secondary accession number(s): Q14CE8, Q2VPI8, Q8NFI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: June 20, 2018
This is version 182 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Pharmaceutical, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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