UniProtKB - P04099 (SCX9_BUTOC)
Protein
Alpha-toxin Bot9
Gene
N/A
Organism
Buthus occitanus tunetanus (Common European scorpion) (Buthus tunetanus)
Status
Functioni
Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin is active against rat Nav1.2/SCN2A and B.germanica Nav1.1 Publication
GO - Molecular functioni
- ion channel inhibitor activity Source: InterPro
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- defense response Source: InterPro
Keywordsi
Molecular function | Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-toxin Bot91 PublicationAlternative name(s): BotIX1 Publication Neurotoxin 9Curated Neurotoxin IXCurated |
Organismi | Buthus occitanus tunetanus (Common European scorpion) (Buthus tunetanus) |
Taxonomic identifieri | 6871 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Buthus › |
Subcellular locationi
Extracellular region or secreted
- Secreted 2 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Toxic dosei
LD50 is 100 ng/kg by subcutaneous injection in mice.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000066737 | 1 – 70 | Alpha-toxin Bot91 PublicationAdd BLAST | 70 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 16 ↔ 68 | PROSITE-ProRule annotation | ||
Disulfide bondi | 20 ↔ 40 | PROSITE-ProRule annotation | ||
Disulfide bondi | 26 ↔ 50 | PROSITE-ProRule annotation | ||
Disulfide bondi | 30 ↔ 52 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.1 Publication
Structurei
3D structure databases
ProteinModelPortali | P04099 |
SMRi | P04099 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 69 | LCN-type CS-alpha/betaPROSITE-ProRule annotationAdd BLAST | 64 |
Domaini
Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (beta/alpha/beta/beta).Curated
Sequence similaritiesi
Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Alpha subfamily.Curated
Family and domain databases
CDDi | cd00107 Knot1, 1 hit |
Gene3Di | 3.30.30.10, 1 hit |
InterProi | View protein in InterPro IPR003614 Scorpion_toxin-like IPR036574 Scorpion_toxin-like_sf IPR018218 Scorpion_toxinL IPR002061 Scorpion_toxinL/defensin |
Pfami | View protein in Pfam PF00537 Toxin_3, 1 hit |
PRINTSi | PR00285 SCORPNTOXIN |
SMARTi | View protein in SMART SM00505 Knot1, 1 hit |
SUPFAMi | SSF57095 SSF57095, 1 hit |
PROSITEi | View protein in PROSITE PS51863 LCN_CSAB, 1 hit |
i Sequence
Sequence statusi: Complete.
P04099-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
AEIKVRDGYI VYPNNCVYHC GLNPYCNDLC TKNGAKSGYC QWLTKWGNAC
60 70
YCYALPEKVP IKDPSYKCYS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 23 | N → D AA sequence (PubMed:6729843).Curated | 1 | |
Sequence conflicti | 32 – 33 | Missing AA sequence (PubMed:6729843).Curated | 2 |
Mass spectrometryi
Sequence databases
PIRi | A05136 |
Similar proteinsi
Cross-referencesi
Sequence databases
PIRi | A05136 |
3D structure databases
ProteinModelPortali | P04099 |
SMRi | P04099 |
ModBasei | Search... |
MobiDBi | Search... |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Family and domain databases
CDDi | cd00107 Knot1, 1 hit |
Gene3Di | 3.30.30.10, 1 hit |
InterProi | View protein in InterPro IPR003614 Scorpion_toxin-like IPR036574 Scorpion_toxin-like_sf IPR018218 Scorpion_toxinL IPR002061 Scorpion_toxinL/defensin |
Pfami | View protein in Pfam PF00537 Toxin_3, 1 hit |
PRINTSi | PR00285 SCORPNTOXIN |
SMARTi | View protein in SMART SM00505 Knot1, 1 hit |
SUPFAMi | SSF57095 SSF57095, 1 hit |
PROSITEi | View protein in PROSITE PS51863 LCN_CSAB, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | SCX9_BUTOC | |
Accessioni | P04099Primary (citable) accession number: P04099 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1986 |
Last sequence update: | February 15, 2017 | |
Last modified: | December 5, 2018 | |
This is version 73 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families