UniProtKB - P04079 (GUAA_ECOLI)
Protein
GMP synthase [glutamine-hydrolyzing]
Gene
guaA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the synthesis of GMP from XMP.
Catalytic activityi
- EC:6.3.5.2
Activity regulationi
Inhibited by 2-fluoroinosine 5'-monophosphate (F-IMP) and by N2-hydroxyguanosine 5'-monophosphate (N2-OH-GMP).1 Publication
: GMP biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).Proteins known to be involved in this subpathway in this organism are:
- GMP synthase [glutamine-hydrolyzing] (guaA), GMP synthase [glutamine-hydrolyzing] (guaA)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 86 | Nucleophile | 1 | |
Active sitei | 181 | 1 | ||
Active sitei | 183 | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 235 – 241 | ATP | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- GMP synthase (glutamine-hydrolyzing) activity Source: EcoCyc
- GMP synthase activity Source: EcoCyc
- identical protein binding Source: IntAct
- pyrophosphatase activity Source: InterPro
GO - Biological processi
- glutamine metabolic process Source: UniProtKB-KW
- GMP biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Ligase |
Biological process | GMP biosynthesis, Purine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:GMP-SYN-MONOMER MetaCyc:GMP-SYN-MONOMER |
BRENDAi | 6.3.5.2, 2026 |
UniPathwayi | UPA00189;UER00296 |
Protein family/group databases
MEROPSi | C26.957 |
Names & Taxonomyi
Protein namesi | Recommended name: GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)Alternative name(s): GMP synthetase Short name: GMPS Glutamine amidotransferase |
Gene namesi | Name:guaA Ordered Locus Names:b2507, JW2491 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000140123 | 1 – 525 | GMP synthase [glutamine-hydrolyzing]Add BLAST | 525 |
Proteomic databases
jPOSTi | P04079 |
PaxDbi | P04079 |
PRIDEi | P04079 |
Expressioni
Inductioni
Activated by cAMP receptor protein (CRP). Repressed by DnaA.
Interactioni
Subunit structurei
Homodimer.
Binary interactionsi
P04079
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-909122,EBI-909122 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259645, 51 interactors |
DIPi | DIP-9852N |
IntActi | P04079, 8 interactors |
STRINGi | 511145.b2507 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P04079 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04079 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 207 | Glutamine amidotransferase type-1Add BLAST | 199 | |
Domaini | 208 – 400 | GMPS ATP-PPaseAdd BLAST | 193 |
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | COG0518, Bacteria COG0519, Bacteria |
HOGENOMi | CLU_014340_0_5_6 |
InParanoidi | P04079 |
PhylomeDBi | P04079 |
Family and domain databases
CDDi | cd01742, GATase1_GMP_Synthase, 1 hit cd01997, GMP_synthase_C, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.40.50.880, 1 hit |
HAMAPi | MF_00344, GMP_synthase, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR017926, GATASE IPR001674, GMP_synth_C IPR004739, GMP_synth_GATase IPR022955, GMP_synthase IPR025777, GMPS_ATP_PPase_dom IPR022310, NAD/GMP_synthase IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00117, GATase, 1 hit PF00958, GMP_synt_C, 1 hit PF02540, NAD_synthase, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR00884, guaA_Cterm, 1 hit TIGR00888, guaA_Nterm, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit PS51553, GMPS_ATP_PPASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P04079-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTENIHKHRI LILDFGSQYT QLVARRVREL GVYCELWAWD VTEAQIRDFN
60 70 80 90 100
PSGIILSGGP ESTTEENSPR APQYVFEAGV PVFGVCYGMQ TMAMQLGGHV
110 120 130 140 150
EASNEREFGY AQVEVVNDSA LVRGIEDALT ADGKPLLDVW MSHGDKVTAI
160 170 180 190 200
PSDFITVAST ESCPFAIMAN EEKRFYGVQF HPEVTHTRQG MRMLERFVRD
210 220 230 240 250
ICQCEALWTP AKIIDDAVAR IREQVGDDKV ILGLSGGVDS SVTAMLLHRA
260 270 280 290 300
IGKNLTCVFV DNGLLRLNEA EQVLDMFGDH FGLNIVHVPA EDRFLSALAG
310 320 330 340 350
ENDPEAKRKI IGRVFVEVFD EEALKLEDVK WLAQGTIYPD VIESAASATG
360 370 380 390 400
KAHVIKSHHN VGGLPKEMKM GLVEPLKELF KDEVRKIGLE LGLPYDMLYR
410 420 430 440 450
HPFPGPGLGV RVLGEVKKEY CDLLRRADAI FIEELRKADL YDKVSQAFTV
460 470 480 490 500
FLPVRSVGVM GDGRKYDWVV SLRAVETIDF MTAHWAHLPY DFLGRVSNRI
510 520
INEVNGISRV VYDISGKPPA TIEWE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 | E → N AA sequence (PubMed:3894345).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M10101 Genomic DNA Translation: AAB18619.1 U00096 Genomic DNA Translation: AAC75560.1 AP009048 Genomic DNA Translation: BAA16394.1 |
PIRi | A24640, SYECGU |
RefSeqi | NP_417002.1, NC_000913.3 WP_000138270.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75560; AAC75560; b2507 BAA16394; BAA16394; BAA16394 |
GeneIDi | 57732391 947334 |
KEGGi | ecj:JW2491 eco:b2507 |
PATRICi | fig|1411691.4.peg.4230 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M10101 Genomic DNA Translation: AAB18619.1 U00096 Genomic DNA Translation: AAC75560.1 AP009048 Genomic DNA Translation: BAA16394.1 |
PIRi | A24640, SYECGU |
RefSeqi | NP_417002.1, NC_000913.3 WP_000138270.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GPM | X-ray | 2.20 | A/B/C/D | 1-525 | [»] | |
SMRi | P04079 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259645, 51 interactors |
DIPi | DIP-9852N |
IntActi | P04079, 8 interactors |
STRINGi | 511145.b2507 |
Chemistry databases
DrugBanki | DB04272, Citric acid |
Protein family/group databases
MEROPSi | C26.957 |
Proteomic databases
jPOSTi | P04079 |
PaxDbi | P04079 |
PRIDEi | P04079 |
Genome annotation databases
EnsemblBacteriai | AAC75560; AAC75560; b2507 BAA16394; BAA16394; BAA16394 |
GeneIDi | 57732391 947334 |
KEGGi | ecj:JW2491 eco:b2507 |
PATRICi | fig|1411691.4.peg.4230 |
Organism-specific databases
EchoBASEi | EB0415 |
Phylogenomic databases
eggNOGi | COG0518, Bacteria COG0519, Bacteria |
HOGENOMi | CLU_014340_0_5_6 |
InParanoidi | P04079 |
PhylomeDBi | P04079 |
Enzyme and pathway databases
UniPathwayi | UPA00189;UER00296 |
BioCyci | EcoCyc:GMP-SYN-MONOMER MetaCyc:GMP-SYN-MONOMER |
BRENDAi | 6.3.5.2, 2026 |
Miscellaneous databases
EvolutionaryTracei | P04079 |
PROi | PR:P04079 |
Family and domain databases
CDDi | cd01742, GATase1_GMP_Synthase, 1 hit cd01997, GMP_synthase_C, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.40.50.880, 1 hit |
HAMAPi | MF_00344, GMP_synthase, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR017926, GATASE IPR001674, GMP_synth_C IPR004739, GMP_synth_GATase IPR022955, GMP_synthase IPR025777, GMPS_ATP_PPase_dom IPR022310, NAD/GMP_synthase IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00117, GATase, 1 hit PF00958, GMP_synt_C, 1 hit PF02540, NAD_synthase, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR00884, guaA_Cterm, 1 hit TIGR00888, guaA_Nterm, 1 hit |
PROSITEi | View protein in PROSITE PS51273, GATASE_TYPE_1, 1 hit PS51553, GMPS_ATP_PPASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GUAA_ECOLI | |
Accessioni | P04079Primary (citable) accession number: P04079 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1986 |
Last sequence update: | January 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 194 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references