UniProtKB - P04074 (AT1A1_SHEEP)
Sodium/potassium-transporting ATPase subunit alpha-1
ATP1A1
Functioni
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
By similarityCatalytic activityi
- EC:7.2.2.13
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 374 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Binding sitei | 485 | ATPBy similarity | 1 | |
Metal bindingi | 715 | MagnesiumBy similarity | 1 | |
Metal bindingi | 719 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- chaperone binding Source: Ensembl
- metal ion binding Source: UniProtKB-KW
- phosphatase activity Source: Ensembl
- P-type sodium:potassium-exchanging transporter activity Source: UniProtKB
- steroid hormone binding Source: Ensembl
GO - Biological processi
- cellular potassium ion homeostasis Source: Ensembl
- cellular response to steroid hormone stimulus Source: Ensembl
- cellular sodium ion homeostasis Source: Ensembl
- membrane repolarization Source: Ensembl
- negative regulation of glucocorticoid biosynthetic process Source: Ensembl
- negative regulation of heart contraction Source: Ensembl
- positive regulation of heart contraction Source: Ensembl
- positive regulation of striated muscle contraction Source: Ensembl
- potassium ion import across plasma membrane Source: Ensembl
- regulation of blood pressure Source: Ensembl
- regulation of sodium ion transport Source: UniProtKB
- regulation of the force of heart contraction Source: Ensembl
- response to glycoside Source: Ensembl
- response to xenobiotic stimulus Source: Ensembl
- sodium ion export across plasma membrane Source: Ensembl
Keywordsi
Molecular function | Translocase |
Biological process | Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium |
Names & Taxonomyi
Protein namesi | Recommended name: Sodium/potassium-transporting ATPase subunit alpha-1 (EC:7.2.2.13)Short name: Na(+)/K(+) ATPase alpha-1 subunit Alternative name(s): Sodium pump subunit alpha-1 |
Gene namesi | Name:ATP1A1 |
Organismi | Ovis aries (Sheep) |
Taxonomic identifieri | 9940 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Basolateral cell membrane By similarity; Multi-pass membrane protein Sequence analysis
- sarcolemma By similarity; Multi-pass membrane protein Sequence analysis
Other locations
- axon By similarity
- Melanosome By similarity
Plasma Membrane
- apical plasma membrane Source: Ensembl
- basolateral plasma membrane Source: UniProtKB
- lateral plasma membrane Source: Ensembl
- plasma membrane Source: UniProtKB
- sodium:potassium-exchanging ATPase complex Source: Ensembl
- T-tubule Source: Ensembl
Other locations
- axon Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB
- melanosome Source: UniProtKB-SubCell
- organelle membrane Source: Ensembl
- postsynaptic density Source: Ensembl
- sperm flagellum Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 6 – 85 | CytoplasmicSequence analysisAdd BLAST | 80 | |
Transmembranei | 86 – 106 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 107 – 129 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 130 – 150 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 151 – 286 | CytoplasmicSequence analysisAdd BLAST | 136 | |
Transmembranei | 287 – 306 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 307 – 318 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 319 – 336 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 337 – 770 | CytoplasmicSequence analysisAdd BLAST | 434 | |
Transmembranei | 771 – 790 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 791 – 800 | ExtracellularSequence analysis | 10 | |
Transmembranei | 801 – 821 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 822 – 841 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 842 – 864 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 865 – 916 | ExtracellularSequence analysisAdd BLAST | 52 | |
Transmembranei | 917 – 936 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 937 – 949 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 950 – 968 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 969 – 983 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 984 – 1004 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1005 – 1021 | CytoplasmicSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Cell membrane, Cell projection, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000002491 | 1 – 5 | By similarity | 5 | |
ChainiPRO_0000002492 | 6 – 1021 | Sodium/potassium-transporting ATPase subunit alpha-1Add BLAST | 1016 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 10 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 16 | Phosphoserine; by PKCBy similarity | 1 | |
Modified residuei | 21 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 38 | PhosphoserineBy similarity | 1 | |
Modified residuei | 45 | PhosphoserineBy similarity | 1 | |
Modified residuei | 226 | PhosphoserineBy similarity | 1 | |
Modified residuei | 258 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 450 | PhosphoserineBy similarity | 1 | |
Modified residuei | 482 | PhosphoserineBy similarity | 1 | |
Modified residuei | 540 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 666 | PhosphoserineBy similarity | 1 | |
Modified residuei | 941 | Phosphoserine; by PKABy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinInteractioni
Subunit structurei
The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.
Interacts with regulatory subunit FXYD1.
Interacts with regulatory subunit FXYD3.
Interacts with SIK1.
Interacts with SLC35G1 and STIM1.
Interacts with CLN3; this interaction regulates the sodium/potassium-transporting ATPase complex localization at the plasma membrane (By similarity).
By similarityGO - Molecular functioni
- chaperone binding Source: Ensembl
Protein-protein interaction databases
STRINGi | 9940.ENSOARP00000021706 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 36 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 80 – 82 | Phosphoinositide-3 kinase bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0203, Eukaryota |
HOGENOMi | CLU_002360_4_3_1 |
OMAi | CYIAYSV |
OrthoDBi | 100699at2759 |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MGKGVGRDKY EPAAVSEHGD KKKAKKERDM DELKKEVSMD DHKLSLDELH
60 70 80 90 100
RKYGTDLNRG LTTARAAEIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSM
110 120 130 140 150
LLWIGAVLCF LAYGIQAATE EEPQNDNLYL GVVLSAVVII TGCFSYYQEA
160 170 180 190 200
KSSKIMESFK NMVPQQALVI RNGEKMSINA EEVVVGDLVE VKGGDRIPAD
210 220 230 240 250
LRIISANGCK VDNSSLTGES EPQTRSPDFT NENPLETRNI AFFSTNCVEG
260 270 280 290 300
TARGIVVYTG DRTVMGRIAT LASGLEGGQT PIAAEIEHFI HIITGVAVFL
310 320 330 340 350
GVSFFILSLI LEYTWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA
360 370 380 390 400
RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT
410 420 430 440 450
TENQSGVSFD KTSATWLALS RIAGLCNRAV FQANQDNLPI LKRAVAGDAS
460 470 480 490 500
ESALLKCIEV CCGSVKEMRE RYAKIVEIPF NSTNKYQLSI HKNANAGEPR
510 520 530 540 550
HLLVMKGAPE RILDRCSSIL IHGKEQPLDE ELKDAFQNAY LELGGLGERV
560 570 580 590 600
LGFCHLMLPD EQFPEGFQFD TDDVNFPVDN LCFVGLISMI DPPRAAVPDA
610 620 630 640 650
VGKCRSAGIK VIMVTGDHPI TAKAIAKGVG IISEGNETVE DIAARLNIPV
660 670 680 690 700
SQVNPRDARA CVVHGSDLKD MTPEQLDDIL KYHTEIVFAR TSPQQKLIIV
710 720 730 740 750
EGCQRQGAIV AVTGDGVNDS PALKKADIGV AMGIAGSDVS KQAADMILLD
760 770 780 790 800
DNFASIVTGV EEGRLIFDNL KKSIAYTLTS NIPEITPFLI FIIANIPLPL
810 820 830 840 850
GTVTILCIDL GTDMVPAISL AYEQAESDIM KRQPRNPQTD KLVNERLISM
860 870 880 890 900
AYGQIGMIQA LGGFFTYFVI MAENGFLPNH LLGIRVTWDD RWINDVEDSY
910 920 930 940 950
GQQWTYEQRK IVEFTCHTAF FVSIVVVQWA DLVICKTRRN SVFQQGMKNK
960 970 980 990 1000
ILIFGLFEET ALAAFLSYCP GMGVALRMYP LKPTWWFCAF PYSLLIFVYD
1010 1020
EVRKLIIRRR PGGWVEKETY Y
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketW5QG59 | W5QG59_SHEEP | Sodium/potassium-transporting ATPas... | ATP1A1 | 1,004 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02813 mRNA Translation: CAA26581.1 X02813 mRNA Translation: CAA26582.1 |
PIRi | A01074, PWSHNA |
RefSeqi | NP_001009360.1, NM_001009360.1 |
Genome annotation databases
Ensembli | ENSOART00000022009; ENSOARP00000021706; ENSOARG00000020213 |
GeneIDi | 443381 |
KEGGi | oas:443381 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02813 mRNA Translation: CAA26581.1 X02813 mRNA Translation: CAA26582.1 |
PIRi | A01074, PWSHNA |
RefSeqi | NP_001009360.1, NM_001009360.1 |
3D structure databases
AlphaFoldDBi | P04074 |
BMRBi | P04074 |
SMRi | P04074 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9940.ENSOARP00000021706 |
Genome annotation databases
Ensembli | ENSOART00000022009; ENSOARP00000021706; ENSOARG00000020213 |
GeneIDi | 443381 |
KEGGi | oas:443381 |
Organism-specific databases
CTDi | 476 |
Phylogenomic databases
eggNOGi | KOG0203, Eukaryota |
HOGENOMi | CLU_002360_4_3_1 |
OMAi | CYIAYSV |
OrthoDBi | 100699at2759 |
Gene expression databases
ExpressionAtlasi | P04074, baseline |
Family and domain databases
CDDi | cd02608, P-type_ATPase_Na-K_like, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR005775, P-type_ATPase_IIC IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01106, ATPase-IIC_X-K, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AT1A1_SHEEP | |
Accessioni | P04074Primary (citable) accession number: P04074 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1986 |
Last sequence update: | November 1, 1986 | |
Last modified: | May 25, 2022 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families