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Protein

Endo-beta-N-acetylglucosaminidase H

Gene
N/A
Organism
Streptomyces plicatus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves asparagine-linked oligomannose and hybrid, but not complex, oligosaccharides from glycoproteins.2 Publications

Catalytic activityi

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei174Proton donor1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH18 Glycoside Hydrolase Family 18

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-beta-N-acetylglucosaminidase H (EC:3.2.1.96)
Alternative name(s):
DI-N-acetylchitobiosyl beta-N-acetylglucosaminidase H
Endoglycosidase H
Short name:
Endo H
Mannosyl-glycoprotein endo-beta-N-acetyl-glucosaminidase H
OrganismiStreptomyces plicatus
Taxonomic identifieri1922 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi172D → A or E: 2% activity. 1 Publication1
Mutagenesisi172D → N: 0.1% activity. 1 Publication1
Mutagenesisi174E → A: Loss of activity. 1 Publication1
Mutagenesisi174E → D or Q: 0.05% activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 42Or 441 PublicationAdd BLAST42
ChainiPRO_000001195843 – 313Endo-beta-N-acetylglucosaminidase HAdd BLAST271

Structurei

Secondary structure

1313
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04067
SMRiP04067
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04067

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 18 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR016289 Endo-Fsp
IPR001223 Glyco_hydro18_cat
IPR001579 Glyco_hydro_18_chit_AS
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00704 Glyco_hydro_18, 1 hit
PIRSFiPIRSF001103 Endo-b-N-acetylglucosaminidase, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS01095 CHITINASE_18, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04067-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFTPVRRRVR TAALALSAAA ALVLGSTAAS GASATPSPAP APAPAPVKQG
60 70 80 90 100
PTSVAYVEVN NNSMLNVGKY TLADGGGNAF DVAVIFAANI NYDTGTKTAY
110 120 130 140 150
LHFNENVQRV LDNAVTQIRP LQQQGIKVLL SVLGNHQGAG FANFPSQQAA
160 170 180 190 200
SAFAKQLSDA VAKYGLDGVD FDDEYAEYGN NGTAQPNDSS FVHLVTALRA
210 220 230 240 250
NMPDKIISLY NIGPAASRLS YGGVDVSDKF DYAWNPYYGT WQVPGIALPK
260 270 280 290 300
AQLSPAAVEI GRTSRSTVAD LARRTVDEGY GVYLTYNLDG GDRTADVSAF
310
TRELYGSEAV RTP
Length:313
Mass (Da):33,052
Last modified:November 1, 1986 - v1
Checksum:i69E25DE1A615CF71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02182 Genomic DNA Translation: AAA26738.1
PIRiA00903 RBSMHP

Similar proteinsi

Cross-referencesi

Web resourcesi

ProZyme product sheet

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02182 Genomic DNA Translation: AAA26738.1
PIRiA00903 RBSMHP

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C3FX-ray2.10A48-312[»]
1C8XX-ray2.00A48-312[»]
1C8YX-ray2.00A48-312[»]
1C90X-ray2.10A/B48-312[»]
1C91X-ray2.10A48-312[»]
1C92X-ray2.10A48-312[»]
1C93X-ray2.10A48-312[»]
1EDTX-ray1.90A43-313[»]
ProteinModelPortaliP04067
SMRiP04067
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH18 Glycoside Hydrolase Family 18

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP04067

Family and domain databases

InterProiView protein in InterPro
IPR016289 Endo-Fsp
IPR001223 Glyco_hydro18_cat
IPR001579 Glyco_hydro_18_chit_AS
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00704 Glyco_hydro_18, 1 hit
PIRSFiPIRSF001103 Endo-b-N-acetylglucosaminidase, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS01095 CHITINASE_18, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEBAG_STRPL
AccessioniPrimary (citable) accession number: P04067
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: May 10, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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