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Protein

Phospholipase A2

Gene

PLA2G1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Calcium; via carbonyl oxygenBy similarity1
Metal bindingi52Calcium; via carbonyl oxygenBy similarity1
Metal bindingi54Calcium; via carbonyl oxygenBy similarity1
Active sitei70By similarity1
Metal bindingi71CalciumBy similarity1
Active sitei121By similarity1

GO - Molecular functioni

  • bile acid binding Source: BHF-UCL
  • calcium-dependent phospholipase A2 activity Source: BHF-UCL
  • calcium ion binding Source: BHF-UCL
  • phospholipase A2 activity Source: BHF-UCL
  • phospholipase A2 activity (consuming 1,2-dipalmitoylphosphatidylcholine) Source: UniProtKB-EC
  • phospholipase A2 activity consuming 1,2-dioleoylphosphatidylethanolamine) Source: UniProtKB-EC
  • signaling receptor binding Source: BHF-UCL

GO - Biological processi

  • actin filament organization Source: ProtInc
  • activation of MAPK activity Source: BHF-UCL
  • activation of phospholipase A2 activity Source: BHF-UCL
  • antibacterial humoral response Source: UniProtKB
  • antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
  • arachidonic acid secretion Source: BHF-UCL
  • cellular response to insulin stimulus Source: BHF-UCL
  • defense response to Gram-positive bacterium Source: UniProtKB
  • fatty acid biosynthetic process Source: BHF-UCL
  • innate immune response in mucosa Source: UniProtKB
  • interleukin-8 production Source: BHF-UCL
  • intracellular signal transduction Source: BHF-UCL
  • leukotriene biosynthetic process Source: BHF-UCL
  • lipid catabolic process Source: BHF-UCL
  • neutrophil chemotaxis Source: BHF-UCL
  • neutrophil mediated immunity Source: BHF-UCL
  • phosphatidic acid biosynthetic process Source: Reactome
  • phosphatidylcholine acyl-chain remodeling Source: Reactome
  • phosphatidylcholine metabolic process Source: BHF-UCL
  • phosphatidylethanolamine acyl-chain remodeling Source: Reactome
  • phosphatidylglycerol acyl-chain remodeling Source: Reactome
  • phosphatidylinositol acyl-chain remodeling Source: Reactome
  • phosphatidylserine acyl-chain remodeling Source: Reactome
  • positive regulation of calcium ion transport into cytosol Source: BHF-UCL
  • positive regulation of cell proliferation Source: BHF-UCL
  • positive regulation of fibroblast proliferation Source: BHF-UCL
  • positive regulation of immune response Source: BHF-UCL
  • positive regulation of NF-kappaB transcription factor activity Source: BHF-UCL
  • positive regulation of protein secretion Source: BHF-UCL
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL
  • regulation of glucose import Source: BHF-UCL
  • signal transduction Source: ProtInc

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1482788 Acyl chain remodelling of PC
R-HSA-1482801 Acyl chain remodelling of PS
R-HSA-1482839 Acyl chain remodelling of PE
R-HSA-1482922 Acyl chain remodelling of PI
R-HSA-1482925 Acyl chain remodelling of PG
R-HSA-1483166 Synthesis of PA
SIGNORiP04054

Chemistry databases

SwissLipidsiSLP:000001083

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Group IB phospholipase A2
Phosphatidylcholine 2-acylhydrolase 1B
Gene namesi
Name:PLA2G1B
Synonyms:PLA2, PLA2A, PPLA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000170890.13
HGNCiHGNC:9030 PLA2G1B
MIMi172410 gene
neXtProtiNX_P04054

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5319
OpenTargetsiENSG00000170890
PharmGKBiPA33361

Chemistry databases

ChEMBLiCHEMBL4426
DrugBankiDB07836 1-DECYL-3-TRIFLUORO ETHYL-SN-GLYCERO-2-PHOSPHOMETHANOL
DB03565 1-O-Octyl-2-Heptylphosphonyl-Sn-Glycero-3-Phosphoethanolamine
DB02659 Cholic Acid
DB02938 Heptanoic Acid
DB03633 Lpc-Ether
DB02448 N-Tridecanoic Acid
DB04552 Niflumic Acid
DB02795 P-Anisic Acid
DB07657 PHOSPHONIC ACID 2-DODECANOYLAMINO-HEXYL ESTER PROPYL ESTER
DB03587 Pyruvoyl Group
DB00795 Sulfasalazine
GuidetoPHARMACOLOGYi1416

Polymorphism and mutation databases

BioMutaiPLA2G1B
DMDMi129404

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 151 PublicationAdd BLAST15
PropeptideiPRO_000002273916 – 22Activation peptide1 Publication7
ChainiPRO_000002274023 – 148Phospholipase A21 PublicationAdd BLAST126

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 991 Publication
Disulfide bondi49 ↔ 1461 Publication
Disulfide bondi51 ↔ 671 Publication
Disulfide bondi66 ↔ 1271 Publication
Disulfide bondi73 ↔ 1201 Publication
Disulfide bondi83 ↔ 1131 Publication
Disulfide bondi106 ↔ 1181 Publication

Post-translational modificationi

Activated by trypsin cleavage in the duodenum. Can also be activated by thrombin or autocatalytically.

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Zymogen

Proteomic databases

PaxDbiP04054
PeptideAtlasiP04054
PRIDEiP04054
ProteomicsDBi51641

Expressioni

Gene expression databases

BgeeiENSG00000170890
CleanExiHS_PLA2G1B
ExpressionAtlasiP04054 baseline and differential
GenevisibleiP04054 HS

Organism-specific databases

HPAiCAB022329
HPA047822
HPA060803

Interactioni

Subunit structurei

Monomer or homodimer (By similarity). The inactive pro-form is a homotrimer.By similarity1 Publication

GO - Molecular functioni

  • signaling receptor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi111336, 2 interactors
IntActiP04054, 1 interactor
STRINGi9606.ENSP00000312286

Chemistry databases

BindingDBiP04054

Structurei

Secondary structure

1148
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 23Combined sources3
Helixi25 – 30Combined sources6
Helixi31 – 33Combined sources3
Helixi40 – 44Combined sources5
Beta strandi45 – 47Combined sources3
Turni48 – 50Combined sources3
Beta strandi51 – 54Combined sources4
Helixi62 – 77Combined sources16
Helixi81 – 83Combined sources3
Turni84 – 87Combined sources4
Beta strandi97 – 100Combined sources4
Beta strandi103 – 106Combined sources4
Helixi112 – 130Combined sources19
Helixi135 – 137Combined sources3
Helixi142 – 145Combined sources4

3D structure databases

ProteinModelPortaliP04054
SMRiP04054
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04054

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4087 Eukaryota
ENOG411283D LUCA
GeneTreeiENSGT00760000119160
HOGENOMiHOG000231749
HOVERGENiHBG008137
InParanoidiP04054
KOiK01047
OMAiWQFRKMI
OrthoDBiEOG091G0UZ3
PhylomeDBiP04054
TreeFamiTF319283

Family and domain databases

CDDicd00125 PLA2c, 1 hit
Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR001211 PLipase_A2
IPR033112 PLipase_A2_Asp_AS
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PANTHERiPTHR11716 PTHR11716, 1 hit
PfamiView protein in Pfam
PF00068 Phospholip_A2_1, 1 hit
PRINTSiPR00389 PHPHLIPASEA2
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00119 PA2_ASP, 1 hit
PS00118 PA2_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04054-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLVLAVLL TVAAADSGIS PRAVWQFRKM IKCVIPGSDP FLEYNNYGCY
60 70 80 90 100
CGLGGSGTPV DELDKCCQTH DNCYDQAKKL DSCKFLLDNP YTHTYSYSCS
110 120 130 140
GSAITCSSKN KECEAFICNC DRNAAICFSK APYNKAHKNL DTKKYCQS
Length:148
Mass (Da):16,360
Last modified:February 1, 1991 - v3
Checksum:iC8E38B2B64AEE8CB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144Missing AA sequence (PubMed:6349696).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01191116D → A. Corresponds to variant dbSNP:rs5632Ensembl.1
Natural variantiVAR_01191389N → K. Corresponds to variant dbSNP:rs5636Ensembl.1
Natural variantiVAR_01191289N → T. Corresponds to variant dbSNP:rs5635Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21056, M22970 Genomic DNA Translation: AAA60107.1
M21054 mRNA Translation: AAA36450.1
AK311830 mRNA Translation: BAG34772.1
AY438977 Genomic DNA Translation: AAR05441.1
AC003982 Genomic DNA Translation: AAB95635.1
CH471054 Genomic DNA Translation: EAW98184.1
BC106725 mRNA Translation: AAI06726.1
BC106726 mRNA Translation: AAI06727.1
CCDSiCCDS9195.1
PIRiC25793 PSHU
RefSeqiNP_000919.1, NM_000928.2
UniGeneiHs.992

Genome annotation databases

EnsembliENST00000308366; ENSP00000312286; ENSG00000170890
GeneIDi5319
KEGGihsa:5319
UCSCiuc001tyd.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPA21B_HUMAN
AccessioniPrimary (citable) accession number: P04054
Secondary accession number(s): B2R4H5, Q3KPI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 1991
Last modified: June 20, 2018
This is version 184 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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