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Protein

DNA nucleotidylexotransferase

Gene

DNTT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).3 Publications

Cofactori

Mg2+1 PublicationNote: Can also utilize other divalent cations, such as Mn2+ and Co2+ (in vitro).By similarityCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi343MagnesiumBy similarity1
Metal bindingi345MagnesiumBy similarity1
Metal bindingi433MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTerminal addition
LigandMagnesium, Metal-binding

Enzyme and pathway databases

SABIO-RKiP04053
SignaLinkiP04053

Names & Taxonomyi

Protein namesi
Recommended name:
DNA nucleotidylexotransferase (EC:2.7.7.313 Publications)
Alternative name(s):
Terminal addition enzyme
Terminal deoxynucleotidyltransferase
Short name:
Terminal transferase1 Publication
Gene namesi
Name:DNTT
Synonyms:TDT1 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000107447.7
HGNCiHGNC:2983 DNTT
MIMi187410 gene
neXtProtiNX_P04053

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi343D → E: Nearly abolishes enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi1791
OpenTargetsiENSG00000107447
PharmGKBiPA27449

Chemistry databases

ChEMBLiCHEMBL4810

Polymorphism and mutation databases

BioMutaiDNTT
DMDMi311033533

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187911 – 509DNA nucleotidylexotransferaseAdd BLAST509

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei134PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04053
PaxDbiP04053
PeptideAtlasiP04053
PRIDEiP04053
ProteomicsDBi51639
51640 [P04053-2]

PTM databases

iPTMnetiP04053
PhosphoSitePlusiP04053

Expressioni

Gene expression databases

BgeeiENSG00000107447 Expressed in 57 organ(s), highest expression level in bone marrow
CleanExiHS_DNTT
GenevisibleiP04053 HS

Organism-specific databases

HPAiHPA044194

Interactioni

Subunit structurei

Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA. Interacts with TRERF1 (PubMed:16371131).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RELQ048643EBI-1220259,EBI-307352

Protein-protein interaction databases

BioGridi108127, 9 interactors
CORUMiP04053
IntActiP04053, 5 interactors
STRINGi9606.ENSP00000360216

Chemistry databases

BindingDBiP04053

Structurei

Secondary structure

1509
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04053
SMRiP04053
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04053

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 124BRCTPROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni151 – 509Mediates interaction with DNTTIP21 PublicationAdd BLAST359
Regioni258 – 262Involved in DNA bindingBy similarity5
Regioni333 – 338Deoxynucleoside triphosphate bindingBy similarity6
Regioni342 – 345Deoxynucleoside triphosphate bindingBy similarity4
Regioni448 – 449Deoxynucleoside triphosphate bindingBy similarity2

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated

Phylogenomic databases

eggNOGiKOG2534 Eukaryota
COG1796 LUCA
GeneTreeiENSGT00530000063002
HOGENOMiHOG000263600
HOVERGENiHBG003670
InParanoidiP04053
KOiK00977
OMAiKTWKAIR
OrthoDBiEOG091G073W
PhylomeDBiP04053
TreeFamiTF103012

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00141 NT_POLXc, 1 hit
Gene3Di1.10.150.110, 1 hit
3.30.210.10, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR002054 DNA-dir_DNA_pol_X
IPR019843 DNA_pol-X_BS
IPR010996 DNA_pol_b-like_N
IPR028207 DNA_pol_B_palm_palm
IPR018944 DNA_pol_lambd_fingers_domain
IPR027421 DNA_pol_lamdba_lyase_dom_sf
IPR037160 DNA_Pol_thumb_sf
IPR022312 DNA_pol_X
IPR029398 PolB_thumb
IPR027292 TdT
IPR001726 TdT/Mu
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF14792 DNA_pol_B_palm, 1 hit
PF14791 DNA_pol_B_thumb, 1 hit
PF10391 DNA_pol_lambd_f, 1 hit
PF14716 HHH_8, 1 hit
PIRSFiPIRSF000817 DNA_NT, 1 hit
PIRSF501175 TDT, 1 hit
PRINTSiPR00869 DNAPOLX
PR00871 DNAPOLXTDT
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00483 POLXc, 1 hit
SUPFAMiSSF47802 SSF47802, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS00522 DNA_POLYMERASE_X, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: P04053-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDPPRASHLS PRKKRPRQTG ALMASSPQDI KFQDLVVFIL EKKMGTTRRA
60 70 80 90 100
FLMELARRKG FRVENELSDS VTHIVAENNS GSDVLEWLQA QKVQVSSQPE
110 120 130 140 150
LLDVSWLIEC IRAGKPVEMT GKHQLVVRRD YSDSTNPGPP KTPPIAVQKI
160 170 180 190 200
SQYACQRRTT LNNCNQIFTD AFDILAENCE FRENEDSCVT FMRAASVLKS
210 220 230 240 250
LPFTIISMKD TEGIPCLGSK VKGIIEEIIE DGESSEVKAV LNDERYQSFK
260 270 280 290 300
LFTSVFGVGL KTSEKWFRMG FRTLSKVRSD KSLKFTRMQK AGFLYYEDLV
310 320 330 340 350
SCVTRAEAEA VSVLVKEAVW AFLPDAFVTM TGGFRRGKKM GHDVDFLITS
360 370 380 390 400
PGSTEDEEQL LQKVMNLWEK KGLLLYYDLV ESTFEKLRLP SRKVDALDHF
410 420 430 440 450
QKCFLIFKLP RQRVDSDQSS WQEGKTWKAI RVDLVLCPYE RRAFALLGWT
460 470 480 490 500
GSRQFERDLR RYATHERKMI LDNHALYDKT KRIFLKAESE EEIFAHLGLD

YIEPWERNA
Length:509
Mass (Da):58,536
Last modified:November 2, 2010 - v3
Checksum:i0EC01E380FA7E5A2
GO
Isoform 2 (identifier: P04053-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     454-454: Missing.

Show »
Length:508
Mass (Da):58,408
Checksum:iC72DABE3ACAEADD1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51F → L in BAD97037 (Ref. 4) Curated1
Sequence conflicti299L → P in BAD97037 (Ref. 4) Curated1
Sequence conflicti329T → A in BAD97037 (Ref. 4) Curated1
Sequence conflicti383T → A in BAD97037 (Ref. 4) Curated1
Sequence conflicti389L → M in BAD97037 (Ref. 4) Curated1
Sequence conflicti452S → SP in AAA53100 (PubMed:2833741).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_058200112R → G5 PublicationsCorresponds to variant dbSNP:rs6584066Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038397454Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11722 mRNA Translation: AAA36726.1
M20703
, M22968, M20694, M20695, M20696, M20697, M20698, M20699, M20700, M20701, M20702 mRNA Translation: AAA53100.1
AB046378 mRNA Translation: BAB72001.1
AK223317 mRNA Translation: BAD97037.1
AL136181 Genomic DNA No translation available.
BC012920 mRNA Translation: AAH12920.1
M21195 Genomic DNA Translation: AAA61137.1
K01919 mRNA Translation: AAA61136.1
CCDSiCCDS44465.1 [P04053-2]
CCDS7447.1 [P04053-1]
PIRiA23924 WXHU
RefSeqiNP_001017520.1, NM_001017520.1 [P04053-2]
NP_004079.3, NM_004088.3 [P04053-1]
UniGeneiHs.534206

Genome annotation databases

EnsembliENST00000371174; ENSP00000360216; ENSG00000107447 [P04053-1]
ENST00000630152; ENSP00000486733; ENSG00000107447 [P04053-2]
GeneIDi1791
KEGGihsa:1791
UCSCiuc001kmf.4 human [P04053-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11722 mRNA Translation: AAA36726.1
M20703
, M22968, M20694, M20695, M20696, M20697, M20698, M20699, M20700, M20701, M20702 mRNA Translation: AAA53100.1
AB046378 mRNA Translation: BAB72001.1
AK223317 mRNA Translation: BAD97037.1
AL136181 Genomic DNA No translation available.
BC012920 mRNA Translation: AAH12920.1
M21195 Genomic DNA Translation: AAA61137.1
K01919 mRNA Translation: AAA61136.1
CCDSiCCDS44465.1 [P04053-2]
CCDS7447.1 [P04053-1]
PIRiA23924 WXHU
RefSeqiNP_001017520.1, NM_001017520.1 [P04053-2]
NP_004079.3, NM_004088.3 [P04053-1]
UniGeneiHs.534206

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2COENMR-A19-125[»]
ProteinModelPortaliP04053
SMRiP04053
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108127, 9 interactors
CORUMiP04053
IntActiP04053, 5 interactors
STRINGi9606.ENSP00000360216

Chemistry databases

BindingDBiP04053
ChEMBLiCHEMBL4810

PTM databases

iPTMnetiP04053
PhosphoSitePlusiP04053

Polymorphism and mutation databases

BioMutaiDNTT
DMDMi311033533

Proteomic databases

MaxQBiP04053
PaxDbiP04053
PeptideAtlasiP04053
PRIDEiP04053
ProteomicsDBi51639
51640 [P04053-2]

Protocols and materials databases

DNASUi1791
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371174; ENSP00000360216; ENSG00000107447 [P04053-1]
ENST00000630152; ENSP00000486733; ENSG00000107447 [P04053-2]
GeneIDi1791
KEGGihsa:1791
UCSCiuc001kmf.4 human [P04053-1]

Organism-specific databases

CTDi1791
DisGeNETi1791
EuPathDBiHostDB:ENSG00000107447.7
GeneCardsiDNTT
H-InvDBiHIX0009072
HGNCiHGNC:2983 DNTT
HPAiHPA044194
MIMi187410 gene
neXtProtiNX_P04053
OpenTargetsiENSG00000107447
PharmGKBiPA27449
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2534 Eukaryota
COG1796 LUCA
GeneTreeiENSGT00530000063002
HOGENOMiHOG000263600
HOVERGENiHBG003670
InParanoidiP04053
KOiK00977
OMAiKTWKAIR
OrthoDBiEOG091G073W
PhylomeDBiP04053
TreeFamiTF103012

Enzyme and pathway databases

SABIO-RKiP04053
SignaLinkiP04053

Miscellaneous databases

EvolutionaryTraceiP04053
GeneWikiiTerminal_deoxynucleotidyl_transferase
GenomeRNAii1791
PROiPR:P04053
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000107447 Expressed in 57 organ(s), highest expression level in bone marrow
CleanExiHS_DNTT
GenevisibleiP04053 HS

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00141 NT_POLXc, 1 hit
Gene3Di1.10.150.110, 1 hit
3.30.210.10, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR002054 DNA-dir_DNA_pol_X
IPR019843 DNA_pol-X_BS
IPR010996 DNA_pol_b-like_N
IPR028207 DNA_pol_B_palm_palm
IPR018944 DNA_pol_lambd_fingers_domain
IPR027421 DNA_pol_lamdba_lyase_dom_sf
IPR037160 DNA_Pol_thumb_sf
IPR022312 DNA_pol_X
IPR029398 PolB_thumb
IPR027292 TdT
IPR001726 TdT/Mu
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF14792 DNA_pol_B_palm, 1 hit
PF14791 DNA_pol_B_thumb, 1 hit
PF10391 DNA_pol_lambd_f, 1 hit
PF14716 HHH_8, 1 hit
PIRSFiPIRSF000817 DNA_NT, 1 hit
PIRSF501175 TDT, 1 hit
PRINTSiPR00869 DNAPOLX
PR00871 DNAPOLXTDT
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00483 POLXc, 1 hit
SUPFAMiSSF47802 SSF47802, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS00522 DNA_POLYMERASE_X, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTDT_HUMAN
AccessioniPrimary (citable) accession number: P04053
Secondary accession number(s): Q53FH1, Q5W103, Q96E50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 2, 2010
Last modified: November 7, 2018
This is version 193 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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