UniProtKB - P04053 (TDT_HUMAN)
Protein
DNA nucleotidylexotransferase
Gene
DNTT
Organism
Homo sapiens (Human)
Status
Functioni
Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.By similarity
Catalytic activityi
- EC:2.7.7.313 Publications
Cofactori
Mg2+1 PublicationNote: Can also utilize other divalent cations, such as Mn2+ and Co2+ (in vitro).By similarityCurated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 343 | MagnesiumBy similarity | 1 | |
Metal bindingi | 345 | MagnesiumBy similarity | 1 | |
Metal bindingi | 433 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- DNA binding Source: InterPro
- DNA-directed DNA polymerase activity Source: InterPro
- DNA nucleotidylexotransferase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- DNA metabolic process Source: UniProtKB
- DNA modification Source: UniProtKB-KW
- double-strand break repair via nonhomologous end joining Source: GO_Central
- response to ATP Source: Ensembl
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Terminal addition |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | P04053 |
SABIO-RKi | P04053 |
SignaLinki | P04053 |
Names & Taxonomyi
Protein namesi | Recommended name: DNA nucleotidylexotransferase (EC:2.7.7.313 Publications)Alternative name(s): Terminal addition enzyme Terminal deoxynucleotidyltransferase Short name: Terminal transferase1 Publication |
Gene namesi | Name:DNTT Synonyms:TDT1 Publication |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000107447.7 |
HGNCi | HGNC:2983, DNTT |
MIMi | 187410, gene |
neXtProti | NX_P04053 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear chromatin Source: Ensembl
- nuclear matrix Source: Ensembl
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- euchromatin Source: Ensembl
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 343 | D → E: Nearly abolishes enzyme activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 1791 |
OpenTargetsi | ENSG00000107447 |
PharmGKBi | PA27449 |
Miscellaneous databases
Pharosi | P04053, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4810 |
DrugBanki | DB02189, 2',3'-Dideoxyadenosine triphosphate |
Polymorphism and mutation databases
BioMutai | DNTT |
DMDMi | 311033533 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218791 | 1 – 509 | DNA nucleotidylexotransferaseAdd BLAST | 509 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 134 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P04053 |
MassIVEi | P04053 |
MaxQBi | P04053 |
PaxDbi | P04053 |
PeptideAtlasi | P04053 |
PRIDEi | P04053 |
ProteomicsDBi | 51639 [P04053-1] 51640 [P04053-2] |
PTM databases
iPTMneti | P04053 |
PhosphoSitePlusi | P04053 |
Expressioni
Gene expression databases
Bgeei | ENSG00000107447, Expressed in bone marrow and 69 other tissues |
Genevisiblei | P04053, HS |
Organism-specific databases
HPAi | ENSG00000107447, Tissue enriched (lymphoid) |
Interactioni
Subunit structurei
Interacts with PRP19 and DNTTIP1.
Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.
Interacts with TRERF1 (PubMed:16371131).
4 PublicationsBinary interactionsi
Hide detailsP04053
With | #Exp. | IntAct |
---|---|---|
MEI4 [A8MW99] | 3 | EBI-1220259,EBI-19944212 |
REL [Q04864] | 3 | EBI-1220259,EBI-307352 |
TCF4 - isoform SEF2-1D [P15884-3] | 3 | EBI-1220259,EBI-13636688 |
Protein-protein interaction databases
BioGRIDi | 108127, 11 interactors |
CORUMi | P04053 |
IntActi | P04053, 5 interactors |
STRINGi | 9606.ENSP00000360216 |
Chemistry databases
BindingDBi | P04053 |
Miscellaneous databases
RNActi | P04053, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P04053 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04053 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 124 | BRCTPROSITE-ProRule annotationAdd BLAST | 98 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 151 – 509 | Mediates interaction with DNTTIP21 PublicationAdd BLAST | 359 | |
Regioni | 258 – 262 | Involved in DNA bindingBy similarity | 5 | |
Regioni | 333 – 338 | Deoxynucleoside triphosphate bindingBy similarity | 6 | |
Regioni | 342 – 345 | Deoxynucleoside triphosphate bindingBy similarity | 4 | |
Regioni | 448 – 449 | Deoxynucleoside triphosphate bindingBy similarity | 2 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 11 – 17 | Nuclear localization signalBy similarity | 7 |
Sequence similaritiesi
Belongs to the DNA polymerase type-X family.Curated
Phylogenomic databases
eggNOGi | KOG2534, Eukaryota |
GeneTreei | ENSGT00940000158584 |
HOGENOMi | CLU_008698_0_0_1 |
InParanoidi | P04053 |
OMAi | YCDLVES |
OrthoDBi | 1212057at2759 |
PhylomeDBi | P04053 |
TreeFami | TF103012 |
Family and domain databases
CDDi | cd00141, NT_POLXc, 1 hit |
Gene3Di | 1.10.150.110, 1 hit 3.30.210.10, 1 hit 3.30.460.10, 1 hit 3.40.50.10190, 1 hit |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR002054, DNA-dir_DNA_pol_X IPR019843, DNA_pol-X_BS IPR010996, DNA_pol_b-like_N IPR028207, DNA_pol_B_palm_palm IPR018944, DNA_pol_lambd_fingers_domain IPR027421, DNA_pol_lamdba_lyase_dom_sf IPR037160, DNA_Pol_thumb_sf IPR022312, DNA_pol_X IPR043519, NT_sf IPR029398, PolB_thumb IPR027292, TdT IPR001726, TdT/Mu |
Pfami | View protein in Pfam PF00533, BRCT, 1 hit PF14792, DNA_pol_B_palm, 1 hit PF14791, DNA_pol_B_thumb, 1 hit PF10391, DNA_pol_lambd_f, 1 hit PF14716, HHH_8, 1 hit |
PIRSFi | PIRSF000817, DNA_NT, 1 hit PIRSF501175, TDT, 1 hit |
PRINTSi | PR00869, DNAPOLX PR00871, DNAPOLXTDT |
SMARTi | View protein in SMART SM00292, BRCT, 1 hit SM00483, POLXc, 1 hit |
SUPFAMi | SSF47802, SSF47802, 1 hit SSF52113, SSF52113, 1 hit SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit PS00522, DNA_POLYMERASE_X, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: P04053-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDPPRASHLS PRKKRPRQTG ALMASSPQDI KFQDLVVFIL EKKMGTTRRA
60 70 80 90 100
FLMELARRKG FRVENELSDS VTHIVAENNS GSDVLEWLQA QKVQVSSQPE
110 120 130 140 150
LLDVSWLIEC IRAGKPVEMT GKHQLVVRRD YSDSTNPGPP KTPPIAVQKI
160 170 180 190 200
SQYACQRRTT LNNCNQIFTD AFDILAENCE FRENEDSCVT FMRAASVLKS
210 220 230 240 250
LPFTIISMKD TEGIPCLGSK VKGIIEEIIE DGESSEVKAV LNDERYQSFK
260 270 280 290 300
LFTSVFGVGL KTSEKWFRMG FRTLSKVRSD KSLKFTRMQK AGFLYYEDLV
310 320 330 340 350
SCVTRAEAEA VSVLVKEAVW AFLPDAFVTM TGGFRRGKKM GHDVDFLITS
360 370 380 390 400
PGSTEDEEQL LQKVMNLWEK KGLLLYYDLV ESTFEKLRLP SRKVDALDHF
410 420 430 440 450
QKCFLIFKLP RQRVDSDQSS WQEGKTWKAI RVDLVLCPYE RRAFALLGWT
460 470 480 490 500
GSRQFERDLR RYATHERKMI LDNHALYDKT KRIFLKAESE EEIFAHLGLD
YIEPWERNA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 | F → L in BAD97037 (Ref. 4) Curated | 1 | |
Sequence conflicti | 299 | L → P in BAD97037 (Ref. 4) Curated | 1 | |
Sequence conflicti | 329 | T → A in BAD97037 (Ref. 4) Curated | 1 | |
Sequence conflicti | 383 | T → A in BAD97037 (Ref. 4) Curated | 1 | |
Sequence conflicti | 389 | L → M in BAD97037 (Ref. 4) Curated | 1 | |
Sequence conflicti | 452 | S → SP in AAA53100 (PubMed:2833741).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_058200 | 112 | R → G5 PublicationsCorresponds to variant dbSNP:rs6584066Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_038397 | 454 | Missing in isoform 2. 2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11722 mRNA Translation: AAA36726.1 M20703 , M22968, M20694, M20695, M20696, M20697, M20698, M20699, M20700, M20701, M20702 mRNA Translation: AAA53100.1 AB046378 mRNA Translation: BAB72001.1 AK223317 mRNA Translation: BAD97037.1 AL136181 Genomic DNA No translation available. BC012920 mRNA Translation: AAH12920.1 M21195 Genomic DNA Translation: AAA61137.1 K01919 mRNA Translation: AAA61136.1 |
CCDSi | CCDS44465.1 [P04053-2] CCDS7447.1 [P04053-1] |
PIRi | A23924, WXHU |
RefSeqi | NP_001017520.1, NM_001017520.1 [P04053-2] NP_004079.3, NM_004088.3 [P04053-1] |
Genome annotation databases
Ensembli | ENST00000371174; ENSP00000360216; ENSG00000107447 [P04053-1] ENST00000630152; ENSP00000486733; ENSG00000107447 [P04053-2] |
GeneIDi | 1791 |
KEGGi | hsa:1791 |
UCSCi | uc001kmf.4, human [P04053-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11722 mRNA Translation: AAA36726.1 M20703 , M22968, M20694, M20695, M20696, M20697, M20698, M20699, M20700, M20701, M20702 mRNA Translation: AAA53100.1 AB046378 mRNA Translation: BAB72001.1 AK223317 mRNA Translation: BAD97037.1 AL136181 Genomic DNA No translation available. BC012920 mRNA Translation: AAH12920.1 M21195 Genomic DNA Translation: AAA61137.1 K01919 mRNA Translation: AAA61136.1 |
CCDSi | CCDS44465.1 [P04053-2] CCDS7447.1 [P04053-1] |
PIRi | A23924, WXHU |
RefSeqi | NP_001017520.1, NM_001017520.1 [P04053-2] NP_004079.3, NM_004088.3 [P04053-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2COE | NMR | - | A | 19-125 | [»] | |
5W4E | X-ray | 2.18 | A/D | 7-22 | [»] | |
SMRi | P04053 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108127, 11 interactors |
CORUMi | P04053 |
IntActi | P04053, 5 interactors |
STRINGi | 9606.ENSP00000360216 |
Chemistry databases
BindingDBi | P04053 |
ChEMBLi | CHEMBL4810 |
DrugBanki | DB02189, 2',3'-Dideoxyadenosine triphosphate |
PTM databases
iPTMneti | P04053 |
PhosphoSitePlusi | P04053 |
Polymorphism and mutation databases
BioMutai | DNTT |
DMDMi | 311033533 |
Proteomic databases
jPOSTi | P04053 |
MassIVEi | P04053 |
MaxQBi | P04053 |
PaxDbi | P04053 |
PeptideAtlasi | P04053 |
PRIDEi | P04053 |
ProteomicsDBi | 51639 [P04053-1] 51640 [P04053-2] |
Protocols and materials databases
Antibodypediai | 16924, 563 antibodies |
DNASUi | 1791 |
Genome annotation databases
Ensembli | ENST00000371174; ENSP00000360216; ENSG00000107447 [P04053-1] ENST00000630152; ENSP00000486733; ENSG00000107447 [P04053-2] |
GeneIDi | 1791 |
KEGGi | hsa:1791 |
UCSCi | uc001kmf.4, human [P04053-1] |
Organism-specific databases
CTDi | 1791 |
DisGeNETi | 1791 |
EuPathDBi | HostDB:ENSG00000107447.7 |
GeneCardsi | DNTT |
HGNCi | HGNC:2983, DNTT |
HPAi | ENSG00000107447, Tissue enriched (lymphoid) |
MIMi | 187410, gene |
neXtProti | NX_P04053 |
OpenTargetsi | ENSG00000107447 |
PharmGKBi | PA27449 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2534, Eukaryota |
GeneTreei | ENSGT00940000158584 |
HOGENOMi | CLU_008698_0_0_1 |
InParanoidi | P04053 |
OMAi | YCDLVES |
OrthoDBi | 1212057at2759 |
PhylomeDBi | P04053 |
TreeFami | TF103012 |
Enzyme and pathway databases
PathwayCommonsi | P04053 |
SABIO-RKi | P04053 |
SignaLinki | P04053 |
Miscellaneous databases
BioGRID-ORCSi | 1791, 8 hits in 845 CRISPR screens |
ChiTaRSi | DNTT, human |
EvolutionaryTracei | P04053 |
GeneWikii | Terminal_deoxynucleotidyl_transferase |
GenomeRNAii | 1791 |
Pharosi | P04053, Tchem |
PROi | PR:P04053 |
RNActi | P04053, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000107447, Expressed in bone marrow and 69 other tissues |
Genevisiblei | P04053, HS |
Family and domain databases
CDDi | cd00141, NT_POLXc, 1 hit |
Gene3Di | 1.10.150.110, 1 hit 3.30.210.10, 1 hit 3.30.460.10, 1 hit 3.40.50.10190, 1 hit |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR002054, DNA-dir_DNA_pol_X IPR019843, DNA_pol-X_BS IPR010996, DNA_pol_b-like_N IPR028207, DNA_pol_B_palm_palm IPR018944, DNA_pol_lambd_fingers_domain IPR027421, DNA_pol_lamdba_lyase_dom_sf IPR037160, DNA_Pol_thumb_sf IPR022312, DNA_pol_X IPR043519, NT_sf IPR029398, PolB_thumb IPR027292, TdT IPR001726, TdT/Mu |
Pfami | View protein in Pfam PF00533, BRCT, 1 hit PF14792, DNA_pol_B_palm, 1 hit PF14791, DNA_pol_B_thumb, 1 hit PF10391, DNA_pol_lambd_f, 1 hit PF14716, HHH_8, 1 hit |
PIRSFi | PIRSF000817, DNA_NT, 1 hit PIRSF501175, TDT, 1 hit |
PRINTSi | PR00869, DNAPOLX PR00871, DNAPOLXTDT |
SMARTi | View protein in SMART SM00292, BRCT, 1 hit SM00483, POLXc, 1 hit |
SUPFAMi | SSF47802, SSF47802, 1 hit SSF52113, SSF52113, 1 hit SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit PS00522, DNA_POLYMERASE_X, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TDT_HUMAN | |
Accessioni | P04053Primary (citable) accession number: P04053 Secondary accession number(s): Q53FH1, Q5W103, Q96E50 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1986 |
Last sequence update: | November 2, 2010 | |
Last modified: | December 2, 2020 | |
This is version 207 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations