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UniProtKB - P04043 (MTD21_STREE)
Protein
Type II methyltransferase M1.DpnII
Gene
dpnM
Organism
Streptococcus pneumoniae
Status
Functioni
An alpha subtype methylase that recognizes the double-stranded sequence 5'-GATC-3', methylates A-2 on both strands, and protects the DNA from cleavage by the DpnII endonuclease.
1 Publication3 PublicationsMiscellaneous
The DpnII restriction system has two different methylases.1 Publication
Catalytic activityi
- a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H+ + S-adenosyl-L-homocysteine2 PublicationsEC:2.1.1.722 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 17 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 21 | S-adenosyl-L-methionine; via amide nitrogenCombined sources | 1 | |
Binding sitei | 46 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 62 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 194 | S-adenosyl-L-methionineCombined sources1 Publication | 1 |
GO - Molecular functioni
- nucleic acid binding Source: InterPro
- site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
GO - Biological processi
- DNA restriction-modification system Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Methyltransferase, Transferase |
Biological process | Restriction system |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.72, 1960 |
Protein family/group databases
REBASEi | 162065, M.Wso11848ORF439P 3636, M1.DpnII |
Names & Taxonomyi
Protein namesi | Recommended name: Type II methyltransferase M1.DpnII1 Publication (EC:2.1.1.721 Publication)Short name: M1.DpnII1 Publication Alternative name(s): Adenine-specific methyltransferase DpnM1 Publication M.DpnII 1 Modification methylase DpnIIA1 Publication |
Gene namesi | Name:dpnM1 Publication |
Organismi | Streptococcus pneumoniae |
Taxonomic identifieri | 1313 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087957 | 1 – 284 | Type II methyltransferase M1.DpnIIAdd BLAST | 284 |
Interactioni
Subunit structurei
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P04043 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P04043 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 177 – 178 | S-adenosyl-L-methionineCombined sources1 Publication | 2 |
Sequence similaritiesi
Belongs to the N(4)/N(6)-methyltransferase family.Curated
Family and domain databases
Gene3Di | 1.10.1020.10, 1 hit |
InterProi | View protein in InterPro IPR023095, Ade_MeTrfase_dom_2 IPR002052, DNA_methylase_N6_adenine_CS IPR012263, M_m6A_EcoRV IPR012327, MeTrfase_D12 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR30481, PTHR30481, 1 hit |
Pfami | View protein in Pfam PF02086, MethyltransfD12, 1 hit |
PIRSFi | PIRSF000398, M_m6A_EcoRV, 1 hit |
PRINTSi | PR00505, D12N6MTFRASE |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00571, dam, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P04043-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIKEIKKVT LQPFTKWTGG KRQLLPVIRE LIPKTYNRYF EPFVGGGALF
60 70 80 90 100
FDLAPKDAVI NDFNAELINC YQQIKDNPQE LIEILKVHQE YNSKEYYLDL
110 120 130 140 150
RSADRDERID MMSEVQRAAR ILYMLRVNFN GLYRVNSKNQ FNVPYGRYKN
160 170 180 190 200
PKIVDEELIS AISVYINNNQ LEIKVGDFEK AIVDVRTGDF VYFDPPYIPL
210 220 230 240 250
SETSAFTSYT HEGFSFADQV RLRDAFKRLS DTGAYVMLSN SSSALVEELY
260 270 280
KDFNIHYVEA TRTNGAKSSS RGKISEIIVT NYEK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11226 Genomic DNA Translation: AAA26872.1 M14339 Genomic DNA Translation: AAA88580.1 |
PIRi | A00556, XYSONA |
RefSeqi | WP_000692845.1, NZ_WNIC01000007.1 |
Genome annotation databases
GeneIDi | 66806924 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11226 Genomic DNA Translation: AAA26872.1 M14339 Genomic DNA Translation: AAA88580.1 |
PIRi | A00556, XYSONA |
RefSeqi | WP_000692845.1, NZ_WNIC01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DPM | X-ray | 1.80 | A | 1-284 | [»] | |
SMRi | P04043 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
REBASEi | 162065, M.Wso11848ORF439P 3636, M1.DpnII |
Genome annotation databases
GeneIDi | 66806924 |
Enzyme and pathway databases
BRENDAi | 2.1.1.72, 1960 |
Miscellaneous databases
EvolutionaryTracei | P04043 |
PROi | PR:P04043 |
Family and domain databases
Gene3Di | 1.10.1020.10, 1 hit |
InterProi | View protein in InterPro IPR023095, Ade_MeTrfase_dom_2 IPR002052, DNA_methylase_N6_adenine_CS IPR012263, M_m6A_EcoRV IPR012327, MeTrfase_D12 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR30481, PTHR30481, 1 hit |
Pfami | View protein in Pfam PF02086, MethyltransfD12, 1 hit |
PIRSFi | PIRSF000398, M_m6A_EcoRV, 1 hit |
PRINTSi | PR00505, D12N6MTFRASE |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00571, dam, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MTD21_STREE | |
Accessioni | P04043Primary (citable) accession number: P04043 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1986 |
Last sequence update: | November 1, 1986 | |
Last modified: | February 23, 2022 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- Restriction enzymes and methylases
Classification of restriction enzymes and methylases and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families