UniProtKB - P04039 (COX8_YEAST)
Cytochrome c oxidase subunit 8, mitochondrial
COX8
Functioni
Miscellaneous
: oxidative phosphorylation Pathwayi
This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.
GO - Molecular functioni
- cytochrome-c oxidase activity Source: InterPro
GO - Biological processi
Keywordsi
Molecular function | Oxidoreductase |
Enzyme and pathway databases
BioCyci | MetaCyc:YLR395C-MONOMER |
UniPathwayi | UPA00705 |
Protein family/group databases
TCDBi | 3.D.4.8.1, the proton-translocating cytochrome oxidase (cox) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome c oxidase subunit 8, mitochondrialAlternative name(s): Cytochrome c oxidase polypeptide VIII |
Gene namesi | Name:COX8 Ordered Locus Names:YLR395C ORF Names:L8084.14 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004387, COX8 |
VEuPathDBi | FungiDB:YLR395C |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Single-pass membrane protein 1 Publication
Mitochondrion
- mitochondrial respiratory chain complex IV Source: SGD
- mitochondrion Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 28 – 51 | Mitochondrial matrix1 PublicationAdd BLAST | 24 | |
Transmembranei | 52 – 73 | Helical1 PublicationAdd BLAST | 22 | |
Topological domaini | 74 | Mitochondrial intermembrane1 Publication | 1 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 27 | Mitochondrion3 PublicationsAdd BLAST | 27 | |
ChainiPRO_0000006173 | 28 – 74 | Cytochrome c oxidase subunit 8, mitochondrialAdd BLAST | 47 | |
PropeptideiPRO_0000006174 | 75 – 78 | 1 Publication | 4 |
Keywords - PTMi
Cleavage on pair of basic residuesProteomic databases
MaxQBi | P04039 |
PaxDbi | P04039 |
PRIDEi | P04039 |
Interactioni
Subunit structurei
Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 12 subunits. The complex is composed of a catalytic core of 3 subunits COX1, COX2 and COX3, encoded in the mitochondrial DNA, and 9 supernumerary subunits COX4, COX5A (or COX5B), COX6, COX7, COX8, COX9, COX12, COX13 and COX26, which are encoded in the nuclear genome (PubMed:7851399, PubMed:30598556, PubMed:30598554). The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with a dimer of ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), resulting in 2 different assemblies (supercomplexes III2IV and III2IV2) (PubMed:10775262, PubMed:10764779, PubMed:30598556, PubMed:30598554).
5 PublicationsProtein-protein interaction databases
BioGRIDi | 31654, 189 interactors |
ComplexPortali | CPX-1721, Mitochondrial respiratory chain complex IV, COX5A variant CPX-1722, Mitochondrial respiratory chain complex IV, COX5B variant |
DIPi | DIP-5445N |
IntActi | P04039, 1 interactor |
STRINGi | 4932.YLR395C |
Miscellaneous databases
RNActi | P04039, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P04039 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4527, Eukaryota |
HOGENOMi | CLU_169812_0_0_1 |
InParanoidi | P04039 |
OMAi | HFKEGVY |
Family and domain databases
CDDi | cd00929, Cyt_c_Oxidase_VIIc, 1 hit |
Gene3Di | 4.10.49.10, 1 hit |
InterProi | View protein in InterPro IPR004202, COX7C/Cox8 IPR036636, COX7C/Cox8_sf |
Pfami | View protein in Pfam PF02935, COX7C, 1 hit |
SUPFAMi | SSF81427, SSF81427, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MLCQQMIRTT AKRSSNIMTR PIIMKRSVHF KDGVYENIPF KVKGRKTPYA
60 70
LSHFGFFAIG FAVPFVACYV QLKKSGAF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 47 | T → C AA sequence (PubMed:6327685).Curated | 1 | |
Sequence conflicti | 68 | C → T AA sequence (PubMed:6327685).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02634 Genomic DNA Translation: AAA34522.1 U19729 Genomic DNA Translation: AAB82353.1 AY558228 Genomic DNA Translation: AAS56554.1 BK006945 Genomic DNA Translation: DAA09696.1 |
PIRi | A25353 |
RefSeqi | NP_013499.1, NM_001182283.1 |
Genome annotation databases
EnsemblFungii | YLR395C_mRNA; YLR395C; YLR395C |
GeneIDi | 851111 |
KEGGi | sce:YLR395C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02634 Genomic DNA Translation: AAA34522.1 U19729 Genomic DNA Translation: AAB82353.1 AY558228 Genomic DNA Translation: AAS56554.1 BK006945 Genomic DNA Translation: DAA09696.1 |
PIRi | A25353 |
RefSeqi | NP_013499.1, NM_001182283.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6GIQ | electron microscopy | 3.23 | h | 1-78 | [»] | |
6HU9 | electron microscopy | 3.35 | h/t | 28-74 | [»] | |
6T0B | electron microscopy | 2.80 | h/u | 28-78 | [»] | |
6T15 | electron microscopy | 3.29 | h | 28-78 | [»] | |
6YMX | electron microscopy | 3.17 | h | 28-78 | [»] | |
6YMY | electron microscopy | 3.41 | h | 28-78 | [»] | |
SMRi | P04039 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31654, 189 interactors |
ComplexPortali | CPX-1721, Mitochondrial respiratory chain complex IV, COX5A variant CPX-1722, Mitochondrial respiratory chain complex IV, COX5B variant |
DIPi | DIP-5445N |
IntActi | P04039, 1 interactor |
STRINGi | 4932.YLR395C |
Protein family/group databases
TCDBi | 3.D.4.8.1, the proton-translocating cytochrome oxidase (cox) superfamily |
Proteomic databases
MaxQBi | P04039 |
PaxDbi | P04039 |
PRIDEi | P04039 |
Genome annotation databases
EnsemblFungii | YLR395C_mRNA; YLR395C; YLR395C |
GeneIDi | 851111 |
KEGGi | sce:YLR395C |
Organism-specific databases
SGDi | S000004387, COX8 |
VEuPathDBi | FungiDB:YLR395C |
Phylogenomic databases
eggNOGi | KOG4527, Eukaryota |
HOGENOMi | CLU_169812_0_0_1 |
InParanoidi | P04039 |
OMAi | HFKEGVY |
Enzyme and pathway databases
UniPathwayi | UPA00705 |
BioCyci | MetaCyc:YLR395C-MONOMER |
Miscellaneous databases
PROi | PR:P04039 |
RNActi | P04039, protein |
Family and domain databases
CDDi | cd00929, Cyt_c_Oxidase_VIIc, 1 hit |
Gene3Di | 4.10.49.10, 1 hit |
InterProi | View protein in InterPro IPR004202, COX7C/Cox8 IPR036636, COX7C/Cox8_sf |
Pfami | View protein in Pfam PF02935, COX7C, 1 hit |
SUPFAMi | SSF81427, SSF81427, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | COX8_YEAST | |
Accessioni | P04039Primary (citable) accession number: P04039 Secondary accession number(s): D6VZ30 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1986 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 173 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families