UniProtKB - P03966 (MYCN_MOUSE)
Protein
N-myc proto-oncogene protein
Gene
Mycn
Organism
Mus musculus (Mouse)
Status
Functioni
Positively regulates the transcription of MYCNOS in neuroblastoma cells.By similarity
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- kinase binding Source: MGI
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- branching morphogenesis of an epithelial tube Source: MGI
- cartilage condensation Source: MGI
- embryonic digit morphogenesis Source: MGI
- embryonic skeletal system morphogenesis Source: MGI
- lung development Source: MGI
- negative regulation of astrocyte differentiation Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of reactive oxygen species metabolic process Source: UniProtKB
- positive regulation of cell death Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of mesenchymal cell proliferation Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- regulation of inner ear auditory receptor cell differentiation Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Names & Taxonomyi
Protein namesi | Recommended name: N-myc proto-oncogene protein |
Gene namesi | Name:Mycn Synonyms:Nmyc, Nmyc1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97357, Mycn |
Subcellular locationi
Nucleus
Nucleus
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127325 | 1 – 462 | N-myc proto-oncogene proteinAdd BLAST | 462 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 259 | Phosphoserine; by CK2By similarity | 1 | |
Modified residuei | 261 | Phosphoserine; by CK2By similarity | 1 |
Post-translational modificationi
Phosphorylated by GSK3-beta which may promote its degradation. Phosphorylated by AURKA.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P03966 |
PRIDEi | P03966 |
ProteomicsDBi | 287648 |
PTM databases
iPTMneti | P03966 |
PhosphoSitePlusi | P03966 |
Expressioni
Developmental stagei
Expressed in the proliferating cells of the developing CNS and the epidermis. In the spinal cord at embryonic days 10.5, 11.5 and 12.5 dpc, expressed in the proliferating cells of the ventricular zone of the neural tube and is expressed at reduced levels in the intermediate zone. At 14.5 dpc, found in regions containing differentiating post-mitotic neurons. In the developing epidermis at 17 dpc, expression is restricted to primary hair germ cells only.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000037169, Expressed in telencephalon and 316 other tissues |
ExpressionAtlasi | P03966, baseline and differential |
Genevisiblei | P03966, MM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX.
Interacts with KDM5A, KDM5B and HUWE1.
Interacts with MYCNOS.
Interacts with AURKA; interaction is phospho-independent and triggers AURKA activation; AURKA competes with FBXW7 for binding to unphosphorylated MYCN but not for binding to unphosphorylated MYCN.
Interacts with FBXW7; FBXW7 competes with AURKA for binding to unphosphorylated MYCN but not for binding to phosphorylated MYCN.
By similarityGO - Molecular functioni
- kinase binding Source: MGI
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 201795, 8 interactors |
DIPi | DIP-49592N |
IntActi | P03966, 4 interactors |
STRINGi | 10090.ENSMUSP00000045993 |
Miscellaneous databases
RNActi | P03966, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 379 – 431 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 19 – 47 | Interaction with AURKABy similarityAdd BLAST | 29 | |
Regioni | 61 – 90 | Interaction with AURKA and FBXW7By similarityAdd BLAST | 30 | |
Regioni | 431 – 452 | Leucine-zipperAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 260 – 276 | Asp/Glu-rich (acidic)Add BLAST | 17 |
Phylogenomic databases
eggNOGi | KOG2588, Eukaryota |
GeneTreei | ENSGT00940000158432 |
HOGENOMi | CLU_052560_0_0_1 |
InParanoidi | P03966 |
OMAi | SCTASTM |
OrthoDBi | 877891at2759 |
PhylomeDBi | P03966 |
TreeFami | TF106001 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR002418, Tscrpt_reg_Myc IPR012682, Tscrpt_reg_Myc_N |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF01056, Myc_N, 1 hit |
PIRSFi | PIRSF001705, Myc_protein, 1 hit |
PRINTSi | PR00044, LEUZIPPRMYC |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
P03966-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPSCTASTMP GMICKNPDLE FDSLQPCFYP DEDDFYFGGP DSTPPGEDIW
60 70 80 90 100
KKFELLPTPP LSPSRAFPEH SPEPSNWATE MLLPEADLWG NPAEEDAFGL
110 120 130 140 150
GGLGGLTPNP VILQDCMWSG FSAREKLERA VNEKLQHGHG PPGVSSACSA
160 170 180 190 200
PGVGASSPGG RALGGSSSAS HTGATLPTDL SHPAAECVDP AVVFPFPVNK
210 220 230 240 250
RESASVPAAP TSAPATSAAV TSVSVPATAP VAAPARAGGR PASSGEAKAL
260 270 280 290 300
STSGEDTLSD SDDEDDEEED EEEEIDVVTV EKRRSSSNNK AVTTFTITVR
310 320 330 340 350
PKTSALGLGR AQPGELILKR CVPIHQQHNY AAPSPYVESE DAPPQKKIKS
360 370 380 390 400
EASPRPLKSV VPAKAKSLSP RNSDSEDSER RRNHNILERQ RRNDLRSSFL
410 420 430 440 450
TLRDHVPELV KNEKAAKVVI LKKATEYVHA LQANEHQLLL EKEKLQARQQ
460
QLLKKIEHAR TC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 28 – 29 | FY → LH in AAA39788 (PubMed:3513190).Curated | 2 | |
Sequence conflicti | 36 | Y → H in AAA39788 (PubMed:3513190).Curated | 1 | |
Sequence conflicti | 246 – 247 | EA → GT in AAA39788 (PubMed:3513190).Curated | 2 | |
Sequence conflicti | 313 | P → L in AAA39788 (PubMed:3513190).Curated | 1 | |
Sequence conflicti | 390 | Q → E in CAA27557 (PubMed:3015591).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12731 Genomic DNA Translation: AAA39788.1 X03919 Genomic DNA Translation: CAA27557.1 M36277 mRNA Translation: AAA39833.1 M29208 Genomic DNA Translation: AAA39830.1 X06993 Genomic DNA No translation available. |
CCDSi | CCDS25818.1 |
PIRi | A01356, TVMSMC A01357, TVMSM2 |
RefSeqi | NP_032735.3, NM_008709.3 |
Genome annotation databases
Ensembli | ENSMUST00000043396; ENSMUSP00000045993; ENSMUSG00000037169 ENSMUST00000130990; ENSMUSP00000114225; ENSMUSG00000037169 |
GeneIDi | 18109 |
KEGGi | mmu:18109 |
UCSCi | uc007nbf.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M12731 Genomic DNA Translation: AAA39788.1 X03919 Genomic DNA Translation: CAA27557.1 M36277 mRNA Translation: AAA39833.1 M29208 Genomic DNA Translation: AAA39830.1 X06993 Genomic DNA No translation available. |
CCDSi | CCDS25818.1 |
PIRi | A01356, TVMSMC A01357, TVMSM2 |
RefSeqi | NP_032735.3, NM_008709.3 |
3D structure databases
SMRi | P03966 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201795, 8 interactors |
DIPi | DIP-49592N |
IntActi | P03966, 4 interactors |
STRINGi | 10090.ENSMUSP00000045993 |
PTM databases
iPTMneti | P03966 |
PhosphoSitePlusi | P03966 |
Proteomic databases
PaxDbi | P03966 |
PRIDEi | P03966 |
ProteomicsDBi | 287648 |
Genome annotation databases
Ensembli | ENSMUST00000043396; ENSMUSP00000045993; ENSMUSG00000037169 ENSMUST00000130990; ENSMUSP00000114225; ENSMUSG00000037169 |
GeneIDi | 18109 |
KEGGi | mmu:18109 |
UCSCi | uc007nbf.2, mouse |
Organism-specific databases
CTDi | 4613 |
MGIi | MGI:97357, Mycn |
Phylogenomic databases
eggNOGi | KOG2588, Eukaryota |
GeneTreei | ENSGT00940000158432 |
HOGENOMi | CLU_052560_0_0_1 |
InParanoidi | P03966 |
OMAi | SCTASTM |
OrthoDBi | 877891at2759 |
PhylomeDBi | P03966 |
TreeFami | TF106001 |
Miscellaneous databases
BioGRID-ORCSi | 18109, 5 hits in 52 CRISPR screens |
ChiTaRSi | Mycn, mouse |
PROi | PR:P03966 |
RNActi | P03966, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037169, Expressed in telencephalon and 316 other tissues |
ExpressionAtlasi | P03966, baseline and differential |
Genevisiblei | P03966, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR002418, Tscrpt_reg_Myc IPR012682, Tscrpt_reg_Myc_N |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF01056, Myc_N, 1 hit |
PIRSFi | PIRSF001705, Myc_protein, 1 hit |
PRINTSi | PR00044, LEUZIPPRMYC |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MYCN_MOUSE | |
Accessioni | P03966Primary (citable) accession number: P03966 Secondary accession number(s): Q61978 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 1986 |
Last sequence update: | December 15, 1998 | |
Last modified: | April 7, 2021 | |
This is version 173 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot