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Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity).By similarity

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Feedback inhibited by tryptophan.By similarity

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE), Aminodeoxychorismate/anthranilate synthase component 2 (pabA), Anthranilate synthase component 1 (trpE)
  2. Anthranilate phosphoribosyltransferase (trpD), Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF), N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC), Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50TryptophanBy similarity1
Metal bindingi343MagnesiumBy similarity1
Binding sitei431ChorismateBy similarity1
Binding sitei451ChorismateBy similarity1
Binding sitei467Chorismate; via amide nitrogenBy similarity1
Metal bindingi480MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU22680-MONOMER
UniPathwayiUPA00035; UER00040

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1 (EC:4.1.3.27)
Short name:
AS
Short name:
ASI
Gene namesi
Name:trpE
Ordered Locus Names:BSU22680
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001540791 – 515Anthranilate synthase component 1Add BLAST515

Proteomic databases

PaxDbiP03963
PRIDEiP03963

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).By similarity

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012461

Structurei

3D structure databases

ProteinModelPortaliP03963
SMRiP03963
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni281 – 283Tryptophan bindingBy similarity3
Regioni316 – 317Chorismate bindingBy similarity2
Regioni465 – 467Chorismate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CRQ Bacteria
COG0147 LUCA
HOGENOMiHOG000025142
InParanoidiP03963
KOiK01657
OMAiIAGTFKR
PhylomeDBiP03963

Family and domain databases

Gene3Di3.60.120.10, 1 hit
InterProiView protein in InterPro
IPR005801 ADC_synthase
IPR019999 Anth_synth_I-like
IPR006805 Anth_synth_I_N
IPR005256 Anth_synth_I_PabB
IPR015890 Chorismate_C
PfamiView protein in Pfam
PF04715 Anth_synt_I_N, 1 hit
PF00425 Chorismate_bind, 1 hit
PRINTSiPR00095 ANTSNTHASEI
SUPFAMiSSF56322 SSF56322, 1 hit
TIGRFAMsiTIGR00564 trpE_most, 1 hit

Sequencei

Sequence statusi: Complete.

P03963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFQSNISAF LEDSLSHHTI PIVETFTVDT LTPIQMIEKL DREITYLLES
60 70 80 90 100
KDDTSTWSRY SFIGLNPFLT IKEEQGRFSA ADQDSKSLYT GNELKEVLNW
110 120 130 140 150
MNTTYKIKTP ELGIPFVGGA VGYLSYDMIP LIEPSVPSHT KETDMEKCML
160 170 180 190 200
FVCRTLIAYD HETKNVHFIQ YARLTGEETK NEKMDVFHQN HLELQNLIEK
210 220 230 240 250
MMDQKNIKEL FLSADSYKTP SFETVSSNYE KSAFMADVEK IKSYIKAGDI
260 270 280 290 300
FQGVLSQKFE VPIKADAFEL YRVLRIVNPS PYMYYMKLLD REIVGSSPER
310 320 330 340 350
LIHVQDGHLE IHPIAGTRKR GADKAEDERL KVELMKDEKE KAEHYMLVDL
360 370 380 390 400
ARNDIGRVAE YGSVSVPEFT KIVSFSHVMH IISVVTGRLK KGVHPVDALM
410 420 430 440 450
SAFPAGTLTG APKIRAMQLL QELEPTPRET YGGCIAYIGF DGNIDSCITI
460 470 480 490 500
RTMSVKNGVA SIQAGAGIVA DSVPEAEYEE SCNKAGALLK TIHIAEDMFH
510
SKEDKADEQI STIVR
Length:515
Mass (Da):58,116
Last modified:July 1, 1993 - v2
Checksum:i33B88D9D80BCD991
GO

Sequence cautioni

The sequence described in PubMed:3924737 differs from that shown. Reason: Frameshift at positions 343 and 402.Curated
The sequence described in PubMed:6425119 differs from that shown. Reason: Frameshift at positions 343 and 402.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80A → P in AAA22865 (PubMed:3924737).Curated1
Sequence conflicti80A → P in AAA20862 (PubMed:6425119).Curated1
Sequence conflicti120A → S in AAA22865 (PubMed:3924737).Curated1
Sequence conflicti120A → S in AAA20862 (PubMed:6425119).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01391 Genomic DNA Translation: AAA22865.1 Frameshift.
M80245 Genomic DNA Translation: AAA20862.1
AL009126 Genomic DNA Translation: CAB14184.1
M27566 Genomic DNA Translation: AAA22875.1
PIRiA01119 NNBS1
RefSeqiNP_390149.1, NC_000964.3
WP_003246124.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14184; CAB14184; BSU22680
GeneIDi939008
KEGGibsu:BSU22680

Entry informationi

Entry nameiTRPE_BACSU
AccessioniPrimary (citable) accession number: P03963
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: July 1, 1993
Last modified: May 23, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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