UniProtKB - P03960 (KDPB_ECOLI)
Protein
Potassium-transporting ATPase ATP-binding subunit
Gene
kdpB
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm (PubMed:2849541, PubMed:8499455, PubMed:23930894). This subunit is responsible for energy coupling to the transport system (PubMed:16354672).4 Publications
Catalytic activityi
- EC:7.2.2.6UniRule annotation1 Publication
Activity regulationi
ATPase activity is inhibited by vanadate, fluorescein isothiocyanate, N,N'-dicyclohexylcarbodiimide and N-ethylmaleimide.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 307 | 4-aspartylphosphate intermediateUniRule annotation | 1 | |
Binding sitei | 344 | ATPUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 348 | ATPUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 395 | ATPUniRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 518 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 522 | MagnesiumUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 377 – 384 | ATPUniRule annotationCombined sources1 Publication | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
- potassium transmembrane transporter activity, phosphorylative mechanism Source: EcoCyc
GO - Biological processi
- cation transmembrane transport Source: EcoCyc
- potassium ion transmembrane transport Source: EcoCyc
- potassium ion transport Source: EcoliWiki
Keywordsi
Molecular function | Translocase |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium |
Enzyme and pathway databases
BioCyci | EcoCyc:KDPB-MONOMER MetaCyc:KDPB-MONOMER |
Protein family/group databases
TCDBi | 3.A.3.7.1, the p-type atpase (p-atpase) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Potassium-transporting ATPase ATP-binding subunitUniRule annotation (EC:7.2.2.6UniRule annotation1 Publication)Alternative name(s): ATP phosphohydrolase [potassium-transporting] B chainUniRule annotation Potassium-binding and translocating subunit BUniRule annotation Potassium-translocating ATPase B chainUniRule annotation |
Gene namesi | Name:kdpBUniRule annotation Ordered Locus Names:b0697, JW0685 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation2 Publications; Multi-pass membrane protein UniRule annotation1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: EcoliWiki
- potassium ion-transporting ATPase complex Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | CytoplasmicCuratedAdd BLAST | 33 | |
Transmembranei | 34 – 54 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 55 – 61 | PeriplasmicCurated | 7 | |
Transmembranei | 62 – 82 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 83 – 218 | CytoplasmicCuratedAdd BLAST | 136 | |
Transmembranei | 219 – 239 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 240 – 253 | PeriplasmicCuratedAdd BLAST | 14 | |
Transmembranei | 254 – 274 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 275 – 587 | CytoplasmicCuratedAdd BLAST | 313 | |
Transmembranei | 588 – 608 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 609 – 615 | PeriplasmicCurated | 7 | |
Transmembranei | 616 – 636 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 637 – 655 | CytoplasmicCuratedAdd BLAST | 19 | |
Transmembranei | 656 – 676 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 677 – 682 | PeriplasmicCurated | 6 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 377 | F → A: Loss of ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 377 | F → Y: Slight decrease in ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 384 | S → A or T: Decrease in ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 395 | K → A: Strong decrease in ATPase activity. 1 Publication | 1 | |
Mutagenesisi | 399 | D → A: Decrease in ATPase activity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB04395, Phosphoaminophosphonic Acid-Adenylate Ester |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046115 | 1 – 682 | Potassium-transporting ATPase ATP-binding subunitAdd BLAST | 682 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P03960 |
PRIDEi | P03960 |
Expressioni
Inductioni
Transcriptionally regulated by the KdpD/KdpE two-component regulatory system.1 Publication
Interactioni
Subunit structurei
The system is composed of three essential subunits: KdpA, KdpB and KdpC (PubMed:2849541, PubMed:9858692). The complex also contains KdpF, a small non-essential subunit (PubMed:10608856). The KdpFABC complex exists as a dimer above concentrations of 30-50 nM, whereas the complex exists as a functional monomer at lower concentrations (PubMed:18298081).
4 PublicationsBinary interactionsi
P03960
With | #Exp. | IntAct |
---|---|---|
kdpC [P03961] | 2 | EBI-1116956,EBI-6997216 |
Protein-protein interaction databases
BioGRIDi | 4263067, 21 interactors |
ComplexPortali | CPX-3564, KdpFABC potassium import complex |
IntActi | P03960, 9 interactors |
MINTi | P03960 |
STRINGi | 511145.b0697 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P03960 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P03960 |
Family & Domainsi
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IA subfamily. [View classification]UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2216, Bacteria |
InParanoidi | P03960 |
PhylomeDBi | P03960 |
Family and domain databases
CDDi | cd02078, P-type_ATPase_K, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
HAMAPi | MF_00285, KdpB, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006391, P-type_ATPase_bsu_IA IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
PANTHERi | PTHR43743, PTHR43743, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01494, ATPase_P-type, 2 hits TIGR01497, kdpB, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
P03960-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRKQLALFE PTLVVQALKE AVKKLNPQAQ WRNPVMFIVW IGSLLTTCIS
60 70 80 90 100
IAMASGAMPG NALFSAAISG WLWITVLFAN FAEALAEGRS KAQANSLKGV
110 120 130 140 150
KKTAFARKLR EPKYGAAADK VPADQLRKGD IVLVEAGDII PCDGEVIEGG
160 170 180 190 200
ASVDESAITG ESAPVIRESG GDFASVTGGT RILSDWLVIE CSVNPGETFL
210 220 230 240 250
DRMIAMVEGA QRRKTPNEIA LTILLIALTI VFLLATATLW PFSAWGGNAV
260 270 280 290 300
SVTVLVALLV CLIPTTIGGL LSAIGVAGMS RMLGANVIAT SGRAVEAAGD
310 320 330 340 350
VDVLLLDKTG TITLGNRQAS EFIPAQGVDE KTLADAAQLA SLADETPEGR
360 370 380 390 400
SIVILAKQRF NLRERDVQSL HATFVPFTAQ SRMSGINIDN RMIRKGSVDA
410 420 430 440 450
IRRHVEANGG HFPTDVDQKV DQVARQGATP LVVVEGSRVL GVIALKDIVK
460 470 480 490 500
GGIKERFAQL RKMGIKTVMI TGDNRLTAAA IAAEAGVDDF LAEATPEAKL
510 520 530 540 550
ALIRQYQAEG RLVAMTGDGT NDAPALAQAD VAVAMNSGTQ AAKEAGNMVD
560 570 580 590 600
LDSNPTKLIE VVHIGKQMLM TRGSLTTFSI ANDVAKYFAI IPAAFAATYP
610 620 630 640 650
QLNALNIMCL HSPDSAILSA VIFNALIIVF LIPLALKGVS YKPLTASAML
660 670 680
RRNLWIYGLG GLLVPFIGIK VIDLLLTVCG LV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 111 – 112 | EP → DA in AAB96336 (PubMed:6146979).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02670 Genomic DNA Translation: AAB96336.1 U00096 Genomic DNA Translation: AAC73791.1 AP009048 Genomic DNA Translation: BAA35354.1 |
PIRi | H64804, PWECBK |
RefSeqi | NP_415225.1, NC_000913.3 WP_000087939.1, NZ_SSZK01000045.1 |
Genome annotation databases
EnsemblBacteriai | AAC73791; AAC73791; b0697 BAA35354; BAA35354; BAA35354 |
GeneIDi | 947450 |
KEGGi | ecj:JW0685 eco:b0697 |
PATRICi | fig|1411691.4.peg.1578 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02670 Genomic DNA Translation: AAB96336.1 U00096 Genomic DNA Translation: AAC73791.1 AP009048 Genomic DNA Translation: BAA35354.1 |
PIRi | H64804, PWECBK |
RefSeqi | NP_415225.1, NC_000913.3 WP_000087939.1, NZ_SSZK01000045.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SVJ | NMR | - | A | 316-451 | [»] | |
1U7Q | NMR | - | A | 316-451 | [»] | |
2A00 | NMR | - | A | 316-451 | [»] | |
2A29 | NMR | - | A | 316-451 | [»] | |
5MRW | X-ray | 2.90 | B/F/J | 9-682 | [»] | |
6HRA | electron microscopy | 3.70 | B | 1-682 | [»] | |
6HRB | electron microscopy | 4.00 | B | 1-682 | [»] | |
SMRi | P03960 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263067, 21 interactors |
ComplexPortali | CPX-3564, KdpFABC potassium import complex |
IntActi | P03960, 9 interactors |
MINTi | P03960 |
STRINGi | 511145.b0697 |
Chemistry databases
DrugBanki | DB04395, Phosphoaminophosphonic Acid-Adenylate Ester |
Protein family/group databases
TCDBi | 3.A.3.7.1, the p-type atpase (p-atpase) superfamily |
Proteomic databases
PaxDbi | P03960 |
PRIDEi | P03960 |
Genome annotation databases
EnsemblBacteriai | AAC73791; AAC73791; b0697 BAA35354; BAA35354; BAA35354 |
GeneIDi | 947450 |
KEGGi | ecj:JW0685 eco:b0697 |
PATRICi | fig|1411691.4.peg.1578 |
Organism-specific databases
EchoBASEi | EB0509 |
Phylogenomic databases
eggNOGi | COG2216, Bacteria |
InParanoidi | P03960 |
PhylomeDBi | P03960 |
Enzyme and pathway databases
BioCyci | EcoCyc:KDPB-MONOMER MetaCyc:KDPB-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P03960 |
PROi | PR:P03960 |
Family and domain databases
CDDi | cd02078, P-type_ATPase_K, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
HAMAPi | MF_00285, KdpB, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006391, P-type_ATPase_bsu_IA IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
PANTHERi | PTHR43743, PTHR43743, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01494, ATPase_P-type, 2 hits TIGR01497, kdpB, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KDPB_ECOLI | |
Accessioni | P03960Primary (citable) accession number: P03960 Secondary accession number(s): P78053, P78145 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 1986 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 193 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families