UniProtKB - P03959 (KDPA_ECOLI)
Potassium-transporting ATPase potassium-binding subunit
kdpA
Functioni
Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm (PubMed:23930894, PubMed:2849541, PubMed:8499455).
This subunit binds the periplasmic potassium ions and delivers the ions to the membrane domain of KdpB through an intramembrane tunnel (PubMed:7896809, PubMed:30478378, PubMed:34272288).
6 PublicationsMiscellaneous
GO - Molecular functioni
- potassium ion binding Source: UniProtKB-UniRule
- P-type potassium transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- cation transmembrane transport Source: EcoCyc
- potassium ion transmembrane transport Source: EcoCyc
- potassium ion transport Source: ComplexPortal
Keywordsi
Biological process | Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10513-MONOMER |
BRENDAi | 7.2.2.6, 2026 |
Protein family/group databases
TCDBi | 3.A.3.7.1, the p-type atpase (p-atpase) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Potassium-transporting ATPase potassium-binding subunitUniRule annotationAlternative name(s): ATP phosphohydrolase [potassium-transporting] A chainUniRule annotation Potassium-binding and translocating subunit AUniRule annotation Potassium-translocating ATPase A chainUniRule annotation |
Gene namesi | Name:kdpAUniRule annotation Ordered Locus Names:b0698, JW0686 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation5 Publications; Multi-pass membrane protein UniRule annotation3 Publications
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- potassium ion-transporting ATPase complex Source: EcoCyc
- potassium:proton antiporter complex Source: ComplexPortal
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 2 | Periplasmic1 Publication1 Publication | 2 | |
Transmembranei | 3 – 27 | HelicalCombined sources1 PublicationAdd BLAST | 25 | |
Topological domaini | 28 – 61 | Cytoplasmic1 Publication1 PublicationAdd BLAST | 34 | |
Transmembranei | 62 – 84 | HelicalCombined sources1 PublicationAdd BLAST | 23 | |
Topological domaini | 85 – 125 | Periplasmic1 Publication1 PublicationAdd BLAST | 41 | |
Transmembranei | 126 – 149 | HelicalCombined sources1 PublicationAdd BLAST | 24 | |
Topological domaini | 150 – 165 | Cytoplasmic1 Publication1 PublicationAdd BLAST | 16 | |
Transmembranei | 166 – 192 | HelicalCombined sources1 PublicationAdd BLAST | 27 | |
Topological domaini | 193 – 249 | Periplasmic1 Publication1 PublicationAdd BLAST | 57 | |
Transmembranei | 250 – 269 | HelicalCombined sources1 PublicationAdd BLAST | 20 | |
Topological domaini | 270 – 279 | Cytoplasmic1 Publication1 Publication | 10 | |
Transmembranei | 280 – 300 | HelicalCombined sources1 PublicationAdd BLAST | 21 | |
Topological domaini | 301 – 356 | Periplasmic1 Publication1 PublicationAdd BLAST | 56 | |
Transmembranei | 357 – 368 | HelicalCombined sources1 PublicationAdd BLAST | 12 | |
Topological domaini | 369 – 414 | Cytoplasmic1 Publication1 PublicationAdd BLAST | 46 | |
Transmembranei | 415 – 435 | HelicalCombined sources1 PublicationAdd BLAST | 21 | |
Topological domaini | 436 – 479 | Periplasmic1 Publication1 PublicationAdd BLAST | 44 | |
Transmembranei | 480 – 501 | HelicalCombined sources1 PublicationAdd BLAST | 22 | |
Topological domaini | 502 – 524 | Cytoplasmic1 Publication1 PublicationAdd BLAST | 23 | |
Transmembranei | 525 – 552 | HelicalCombined sources1 PublicationAdd BLAST | 28 | |
Topological domaini | 553 – 557 | Periplasmic1 Publication1 Publication | 5 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 232 | G → A or S: Decrease in K(+) affinity and loss of cation selectivity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166494 | 1 – 557 | Potassium-transporting ATPase potassium-binding subunitAdd BLAST | 557 |
Proteomic databases
PaxDbi | P03959 |
PRIDEi | P03959 |
Expressioni
Inductioni
Interactioni
Subunit structurei
The system is composed of three essential subunits: KdpA, KdpB and KdpC (PubMed:2849541, PubMed:9858692, PubMed:28636601, PubMed:30478378). The complex also contains KdpF, a small non-essential subunit (PubMed:10608856, PubMed:28636601, PubMed:30478378). The KdpFABC complex exists as a dimer above concentrations of 30-50 nM, whereas the complex exists as a functional monomer at lower concentrations (PubMed:18298081).
6 PublicationsProtein-protein interaction databases
BioGRIDi | 4259932, 35 interactors |
ComplexPortali | CPX-3564, KdpFABC potassium import complex |
IntActi | P03959, 3 interactors |
STRINGi | 511145.b0698 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P03959 |
SMRi | P03959 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2060, Bacteria |
HOGENOMi | CLU_018614_3_0_6 |
InParanoidi | P03959 |
OMAi | RQGWAIL |
PhylomeDBi | P03959 |
Family and domain databases
HAMAPi | MF_00275, KdpA, 1 hit |
InterProi | View protein in InterPro IPR004623, KdpA |
PANTHERi | PTHR30607, PTHR30607, 1 hit |
Pfami | View protein in Pfam PF03814, KdpA, 1 hit |
PIRSFi | PIRSF001294, K_ATPaseA, 1 hit |
TIGRFAMsi | TIGR00680, kdpA, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAAQGFLLIA TFLLVLMVLA RPLGSGLARL INDIPLPGTT GVERVLFRAL
60 70 80 90 100
GVSDREMNWK QYLCAILGLN MLGLAVLFFM LLGQHYLPLN PQQLPGLSWD
110 120 130 140 150
LALNTAVSFV TNTNWQSYSG ETTLSYFSQM AGLTVQNFLS AASGIAVIFA
160 170 180 190 200
LIRAFTRQSM STLGNAWVDL LRITLWVLVP VALLIALFFI QQGALQNFLP
210 220 230 240 250
YQAVNTVEGA QQLLPMGPVA SQEAIKMLGT NGGGFFNANS SHPFENPTAL
260 270 280 290 300
TNFVQMLAIF LIPTALCFAF GEVMGDRRQG RMLLWAMSVI FVICVGVVMW
310 320 330 340 350
AEVQGNPHLL ALGTDSSINM EGKESRFGVL VSSLFAVVTT AASCGAVIAM
360 370 380 390 400
HDSFTALGGM VPMWLMQIGE VVFGGVGSGL YGMMLFVLLA VFIAGLMIGR
410 420 430 440 450
TPEYLGKKID VREMKLTALA ILVTPTLVLM GAALAMMTDA GRSAMLNPGP
460 470 480 490 500
HGFSEVLYAV SSAANNNGSA FAGLSANSPF WNCLLAFCMF VGRFGVIIPV
510 520 530 540 550
MAIAGSLVSK KSQAASSGTL PTHGPLFVGL LIGTVLLVGA LTFIPALALG
PVAEYLS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02670 Genomic DNA Translation: AAB96335.1 U00096 Genomic DNA Translation: AAC73792.1 AP009048 Genomic DNA Translation: BAA35356.1 |
PIRi | A01071, PWECAK |
RefSeqi | NP_415226.1, NC_000913.3 WP_000741129.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73792; AAC73792; b0698 BAA35356; BAA35356; BAA35356 |
GeneIDi | 946045 |
KEGGi | ecj:JW0686 eco:b0698 |
PATRICi | fig|1411691.4.peg.1577 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02670 Genomic DNA Translation: AAB96335.1 U00096 Genomic DNA Translation: AAC73792.1 AP009048 Genomic DNA Translation: BAA35356.1 |
PIRi | A01071, PWECAK |
RefSeqi | NP_415226.1, NC_000913.3 WP_000741129.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5MRW | X-ray | 2.90 | A/E/I | 1-557 | [»] | |
6HRA | electron microscopy | 3.70 | A | 1-557 | [»] | |
6HRB | electron microscopy | 4.00 | A | 1-557 | [»] | |
7BGY | electron microscopy | 2.90 | A | 1-557 | [»] | |
7BH1 | electron microscopy | 3.38 | A | 1-557 | [»] | |
7BH2 | electron microscopy | 3.00 | A | 1-557 | [»] | |
7LC3 | electron microscopy | 3.23 | A | 1-557 | [»] | |
7LC6 | electron microscopy | 3.70 | A | 1-557 | [»] | |
AlphaFoldDBi | P03959 | |||||
SMRi | P03959 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259932, 35 interactors |
ComplexPortali | CPX-3564, KdpFABC potassium import complex |
IntActi | P03959, 3 interactors |
STRINGi | 511145.b0698 |
Protein family/group databases
TCDBi | 3.A.3.7.1, the p-type atpase (p-atpase) superfamily |
Proteomic databases
PaxDbi | P03959 |
PRIDEi | P03959 |
Protocols and materials databases
DNASUi | 946045 |
Genome annotation databases
EnsemblBacteriai | AAC73792; AAC73792; b0698 BAA35356; BAA35356; BAA35356 |
GeneIDi | 946045 |
KEGGi | ecj:JW0686 eco:b0698 |
PATRICi | fig|1411691.4.peg.1577 |
Organism-specific databases
EchoBASEi | EB0508 |
Phylogenomic databases
eggNOGi | COG2060, Bacteria |
HOGENOMi | CLU_018614_3_0_6 |
InParanoidi | P03959 |
OMAi | RQGWAIL |
PhylomeDBi | P03959 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10513-MONOMER |
BRENDAi | 7.2.2.6, 2026 |
Miscellaneous databases
PROi | PR:P03959 |
Family and domain databases
HAMAPi | MF_00275, KdpA, 1 hit |
InterProi | View protein in InterPro IPR004623, KdpA |
PANTHERi | PTHR30607, PTHR30607, 1 hit |
Pfami | View protein in Pfam PF03814, KdpA, 1 hit |
PIRSFi | PIRSF001294, K_ATPaseA, 1 hit |
TIGRFAMsi | TIGR00680, kdpA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KDPA_ECOLI | |
Accessioni | P03959Primary (citable) accession number: P03959 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 1986 |
Last sequence update: | August 13, 1987 | |
Last modified: | May 25, 2022 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families