UniProtKB - P03953 (CFAD_MOUSE)
Protein
Complement factor D
Gene
Cfd
Organism
Mus musculus (Mouse)
Status
Functioni
Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Catalytic activityi
- Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor. EC:3.4.21.46
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 66 | Charge relay system | 1 | |
Active sitei | 115 | Charge relay system | 1 | |
Active sitei | 209 | Charge relay system | 1 |
GO - Molecular functioni
- endopeptidase activity Source: MGI
- serine-type endopeptidase activity Source: UniProtKB-EC
GO - Biological processi
- complement activation, alternative pathway Source: MGI
- Notch signaling pathway Source: MGI
- response to bacterium Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Complement alternate pathway, Immunity, Innate immunity |
Enzyme and pathway databases
BRENDAi | 3.4.21.46, 3474 |
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-173736, Alternative complement activation R-MMU-6798695, Neutrophil degranulation |
Protein family/group databases
MEROPSi | S01.191 |
Names & Taxonomyi
Protein namesi | Recommended name: Complement factor D (EC:3.4.21.46)Alternative name(s): 28 kDa adipocyte protein Adipsin C3 convertase activator Properdin factor D |
Gene namesi | Name:Cfd Synonyms:Adn, Df |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:87931, Cfd |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular space Source: MGI
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
PropeptideiPRO_0000027562 | 21 – 25 | Activation peptideSequence analysis | 5 | |
ChainiPRO_0000027563 | 26 – 259 | Complement factor DAdd BLAST | 234 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 46 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 51 ↔ 67 | PROSITE-ProRule annotation | ||
Glycosylationi | 124 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 149 ↔ 215 | PROSITE-ProRule annotation | ||
Glycosylationi | 176 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 180 ↔ 196 | PROSITE-ProRule annotation | ||
Disulfide bondi | 205 ↔ 230 | PROSITE-ProRule annotation | ||
Glycosylationi | 251 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 256 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
N-glycosylated.2 Publications
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
CPTACi | non-CPTAC-3697 |
PaxDbi | P03953 |
PRIDEi | P03953 |
PTM databases
GlyGeni | P03953, 5 sites |
iPTMneti | P03953 |
PhosphoSitePlusi | P03953 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000061780, Expressed in epididymal fat pad and 128 other tissues |
Genevisiblei | P03953, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 197991, 4 interactors |
IntActi | P03953, 2 interactors |
MINTi | P03953 |
STRINGi | 10090.ENSMUSP00000056836 |
Miscellaneous databases
RNActi | P03953, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P03953 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 254 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 229 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000162255 |
HOGENOMi | CLU_006842_1_0_1 |
InParanoidi | P03953 |
OMAi | NMICAHK |
PhylomeDBi | P03953 |
TreeFami | TF333630 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR037561, Complement_factor_D IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
PANTHERi | PTHR24271:SF54, PTHR24271:SF54, 1 hit |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: P03953-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHSSVYFVAL VILGAAVCAA QPRGRILGGQ EAAAHARPYM ASVQVNGTHV
60 70 80 90 100
CGGTLLDEQW VLSAAHCMDG VTDDDSVQVL LGAHSLSAPE PYKRWYDVQS
110 120 130 140 150
VVPHPGSRPD SLEDDLILFK LSQNASLGPH VRPLPLQYED KEVEPGTLCD
160 170 180 190 200
VAGWGVVTHA GRRPDVLHQL RVSIMNRTTC NLRTYHDGVV TINMMCAESN
210 220 230 240 250
RRDTCRGDSG SPLVCGDAVE GVVTWGSRVC GNGKKPGVYT RVSSYRMWIE
NITNGNMTS
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005382 | 20 | Missing in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11768 mRNA Translation: AAA40486.1 X04673 Genomic DNA Translation: CAA28378.1 X04673 Genomic DNA Translation: CAA28379.1 M13386 Genomic DNA Translation: AAA37262.1 |
CCDSi | CCDS23995.1 [P03953-1] CCDS88051.1 [P03953-2] |
PIRi | C25952, WMMS28 |
RefSeqi | NP_001278844.1, NM_001291915.2 [P03953-2] NP_038487.1, NM_013459.4 [P03953-1] |
Genome annotation databases
Ensembli | ENSMUST00000061653; ENSMUSP00000056836; ENSMUSG00000061780 [P03953-1] ENSMUST00000217837; ENSMUSP00000151894; ENSMUSG00000061780 [P03953-2] |
GeneIDi | 11537 |
KEGGi | mmu:11537 |
UCSCi | uc007gaj.3, mouse [P03953-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11768 mRNA Translation: AAA40486.1 X04673 Genomic DNA Translation: CAA28378.1 X04673 Genomic DNA Translation: CAA28379.1 M13386 Genomic DNA Translation: AAA37262.1 |
CCDSi | CCDS23995.1 [P03953-1] CCDS88051.1 [P03953-2] |
PIRi | C25952, WMMS28 |
RefSeqi | NP_001278844.1, NM_001291915.2 [P03953-2] NP_038487.1, NM_013459.4 [P03953-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5FCR | X-ray | 1.25 | A/B/C/D | 26-259 | [»] | |
SMRi | P03953 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 197991, 4 interactors |
IntActi | P03953, 2 interactors |
MINTi | P03953 |
STRINGi | 10090.ENSMUSP00000056836 |
Protein family/group databases
MEROPSi | S01.191 |
PTM databases
GlyGeni | P03953, 5 sites |
iPTMneti | P03953 |
PhosphoSitePlusi | P03953 |
Proteomic databases
CPTACi | non-CPTAC-3697 |
PaxDbi | P03953 |
PRIDEi | P03953 |
Protocols and materials databases
Antibodypediai | 4290, 464 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000061653; ENSMUSP00000056836; ENSMUSG00000061780 [P03953-1] ENSMUST00000217837; ENSMUSP00000151894; ENSMUSG00000061780 [P03953-2] |
GeneIDi | 11537 |
KEGGi | mmu:11537 |
UCSCi | uc007gaj.3, mouse [P03953-1] |
Organism-specific databases
CTDi | 1675 |
MGIi | MGI:87931, Cfd |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000162255 |
HOGENOMi | CLU_006842_1_0_1 |
InParanoidi | P03953 |
OMAi | NMICAHK |
PhylomeDBi | P03953 |
TreeFami | TF333630 |
Enzyme and pathway databases
BRENDAi | 3.4.21.46, 3474 |
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-173736, Alternative complement activation R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 11537, 1 hit in 17 CRISPR screens |
ChiTaRSi | Cfd, mouse |
PROi | PR:P03953 |
RNActi | P03953, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000061780, Expressed in epididymal fat pad and 128 other tissues |
Genevisiblei | P03953, MM |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR037561, Complement_factor_D IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
PANTHERi | PTHR24271:SF54, PTHR24271:SF54, 1 hit |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CFAD_MOUSE | |
Accessioni | P03953Primary (citable) accession number: P03953 Secondary accession number(s): Q61280 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 1986 |
Last sequence update: | October 23, 1986 | |
Last modified: | December 2, 2020 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries