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Protein

Steroid 21-hydroxylase

Gene

Cyp21

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.By similarity

Catalytic activityi

A C(21) steroid + [reduced NADPH--hemoprotein reductase] + O2 = a 21-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi420Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processSteroidogenesis
LigandHeme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 21-hydroxylase (EC:1.14.14.16By similarity)
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene namesi
Name:Cyp21
Synonyms:Cyp21a-1, Cyp21a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88591 Cyp21a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519771 – 487Steroid 21-hydroxylaseAdd BLAST487

Proteomic databases

MaxQBiP03940
PaxDbiP03940
PRIDEiP03940

PTM databases

iPTMnetiP03940
PhosphoSitePlusiP03940

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025223

Structurei

3D structure databases

ProteinModelPortaliP03940
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni334 – 350Steroid-bindingBy similarityAdd BLAST17

Domaini

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
HOVERGENiHBG106944
InParanoidiP03940
KOiK00513
PhylomeDBiP03940
TreeFamiTF105095

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P03940-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL
60 70 80 90 100
GLTQKLGPIY RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM
110 120 130 140 150
DLDLSLGDYS LMWKAHKKLS RSALMLGMRD SMEPLIEQLT QEFCERMRAQ
160 170 180 190 200
AGTPVAIHKE FSFLTCSIIS CLTFGDKDST LVQTLHDCVQ DLLQAWNHWS
210 220 230 240 250
IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK DSLVAGQWKD
260 270 280 290 300
MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA
310 320 330 340 350
FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV
360 370 380 390 400
VPLALPHRAT RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR
410 420 430 440 450
FLEPGKNPRT PSFGCGARVC LGEPLARLEL FVVLARLLQA FTLLPPPDGT
460 470 480
LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP QDQGERP
Length:487
Mass (Da):55,328
Last modified:April 26, 2005 - v3
Checksum:iCAD32B606004449F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J048A0A0R4J048_MOUSE
Cytochrome P450, family 21, subfami...
Cyp21a1 mCG_134597
487Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31 – 32PP → LR in AAA37114 (PubMed:3491986).Curated2
Sequence conflicti66M → L in AAA37114 (PubMed:3491986).Curated1
Sequence conflicti218L → F in BAA31153 (Ref. 3) Curated1
Sequence conflicti238Q → R in BAA31153 (Ref. 3) Curated1
Sequence conflicti238Q → R in AAC05278 (PubMed:14656967).Curated1
Sequence conflicti241D → E in AAA37114 (PubMed:3491986).Curated1
Sequence conflicti314E → A in AAA37114 (PubMed:3491986).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15009 Genomic DNA Translation: AAA37114.1
M64933 Genomic DNA Translation: AAA40118.1
AB015623 Genomic DNA Translation: BAA31153.1
AF049850 Genomic DNA Translation: AAC05278.1
BC127167 mRNA Translation: AAI27168.1
CCDSiCCDS28658.1
PIRiA26660
S54785
RefSeqiNP_034125.2, NM_009995.2
UniGeneiMm.440407

Genome annotation databases

GeneIDi13079
KEGGimmu:13079
UCSCiuc008cdo.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15009 Genomic DNA Translation: AAA37114.1
M64933 Genomic DNA Translation: AAA40118.1
AB015623 Genomic DNA Translation: BAA31153.1
AF049850 Genomic DNA Translation: AAC05278.1
BC127167 mRNA Translation: AAI27168.1
CCDSiCCDS28658.1
PIRiA26660
S54785
RefSeqiNP_034125.2, NM_009995.2
UniGeneiMm.440407

3D structure databases

ProteinModelPortaliP03940
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025223

PTM databases

iPTMnetiP03940
PhosphoSitePlusiP03940

Proteomic databases

MaxQBiP03940
PaxDbiP03940
PRIDEiP03940

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13079
KEGGimmu:13079
UCSCiuc008cdo.2 mouse

Organism-specific databases

CTDi13079
MGIiMGI:88591 Cyp21a1

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
HOVERGENiHBG106944
InParanoidiP03940
KOiK00513
PhylomeDBiP03940
TreeFamiTF105095

Miscellaneous databases

PROiPR:P03940
SOURCEiSearch...

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCP21A_MOUSE
AccessioniPrimary (citable) accession number: P03940
Secondary accession number(s): A0JP50
, O88304, Q64510, Q9QX14
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 1986
Last sequence update: April 26, 2005
Last modified: June 20, 2018
This is version 152 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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