UniProtKB - P03879 (MBI4_YEAST)
Intron-encoded RNA maturase bI4
BI4
Functioni
Mitochondrial mRNA maturase required for splicing of intron 4 of the cytochrome b (COB) gene, containing its own coding sequence, and intron 4 in COX1, coding for the related homing endonuclease aI4. In vivo splicing requires in addition the imported mitochondrial leucyl-tRNA synthetase NAM2. Both proteins seem to stimulate the intrinsic ribozyme activity of intron bI4 through binding to and stabilizing specific secondary and tertiary structure elements in the RNA.
4 PublicationsMiscellaneous
GO - Molecular functioni
- endonuclease activity Source: InterPro
- RNA binding Source: SGD
GO - Biological processi
- Group I intron splicing Source: SGD
- mitochondrial mRNA processing Source: SGD
- mRNA processing Source: ComplexPortal
Keywordsi
Molecular function | Hydrolase |
Biological process | mRNA processing, mRNA splicing |
Names & Taxonomyi
Protein namesi | Recommended name: Intron-encoded RNA maturase bI4Alternative name(s): RNA maturase SCbI4 Cleaved into the following 2 chains: |
Gene namesi | Name:BI4 Synonyms:SCBI4 Ordered Locus Names:Q0120 |
Encoded oni | Mitochondrion |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000007273, BI4 |
VEuPathDBi | FungiDB:Q0120 |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000014020 | 1 – ? | Truncated, nonfunctional cytochrome b | ||
ChainiPRO_0000014021 | ? – 638 | RNA maturase bI4 |
Post-translational modificationi
Proteomic databases
PaxDbi | P03879 |
PRIDEi | P03879 |
Interactioni
Subunit structurei
Forms a ternary complex with intron derived RNA and the imported mitochondrial leucyl-tRNA synthetase NAM2. The proteins do not interact directly with each other.
Protein-protein interaction databases
BioGRIDi | 34778, 8 interactors |
ComplexPortali | CPX-1314, bI4 intron splicing factor complex |
IntActi | P03879, 1 interactor |
STRINGi | 4932.Q0120 |
Miscellaneous databases
RNActi | P03879, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 253 | COB exons 1 to 4 encodedAdd BLAST | 253 | |
Regioni | 253 – 638 | COB intron 4 encodedAdd BLAST | 386 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4663, Eukaryota |
GeneTreei | ENSGT00390000017948 |
HOGENOMi | CLU_429071_0_0_1 |
InParanoidi | P03879 |
Family and domain databases
CDDi | cd00284, Cytochrome_b_N, 1 hit |
Gene3Di | 1.20.810.10, 1 hit 3.10.28.10, 2 hits |
InterProi | View protein in InterPro IPR005797, Cyt_b/b6_N IPR027387, Cytb/b6-like_sf IPR016174, Di-haem_cyt_TM IPR027434, Homing_endonucl IPR004860, LAGLIDADG_2 |
Pfami | View protein in Pfam PF00033, Cytochrome_B, 1 hit PF00961, LAGLIDADG_1, 2 hits |
SUPFAMi | SSF55608, SSF55608, 2 hits SSF81342, SSF81342, 1 hit |
PROSITEi | View protein in PROSITE PS51002, CYTB_NTER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAFRKSNVYL SLVNSYIIDS PQPSSINYWW NMGSLLGLCL VIQIVTGIFM
60 70 80 90 100
AMHYSSNIEL AFSSVEHIMR DVHNGYILRY LHANGASFFF MVMFMHMAKG
110 120 130 140 150
LYYGSYRSPR VTLWNVGVII FILTIATAFL GYCCVYGQMS HWGATVITNL
160 170 180 190 200
FSAIPFVGND IVSWLWGGFS VSNPTIQRFF ALHYLVPFII AAMVIMHLMA
210 220 230 240 250
LHIHGSSNPL GITGNLDRIP MHSYFIFKDL VTVFLFMLIL ALFVFYSPNT
260 270 280 290 300
LGQNMALLLI TYVINILCAV CWKSLFIKYQ WKIYNKTTYY FIIQNILNTK
310 320 330 340 350
QLNNFVLKFN WTKQYNKMNI VSDLFNPNRV KYYYKEDNQQ VTNMNSSNTH
360 370 380 390 400
LTSNKKNLLV DTSETTRTTK NKFNYLLNIF NMKKMNQIIT KRHYSIYKDS
410 420 430 440 450
NIRFNQWLAG LIDGDGYFCI TKNKYASCEI TVELKDEKML RQIQDKFGGS
460 470 480 490 500
VKLRSGVKAI RYRLQNKEGM IKLINAVNGN IRNSKRLVQF NKVCILLNID
510 520 530 540 550
FKEPIKLTKD NAWFMGFFDA DGTINYYYSG KLKIRPQLTI SVTNKYLHDV
560 570 580 590 600
EYYREVFGGN IYFDKAKNGY FKWSINNKEL HNIFYTYNKS CPSKSNKGKR
610 620 630
LFLIDKFYYL YDLLAFKAPH NTALYKAWLK FNEKWNNN
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 370 | K → E in CAB43023 (PubMed:6253454).Curated | 1 | |
Sequence conflicti | 390 | T → L in AAB21125 (PubMed:1310149).Curated | 1 | |
Sequence conflicti | 433 | E → K in AAB21125 (PubMed:1310149).Curated | 1 | |
Sequence conflicti | 459 | A → T in CAB43023 (PubMed:6253454).Curated | 1 | |
Sequence conflicti | 465 | Q → K in AAB21125 (PubMed:1310149).Curated | 1 | |
Sequence conflicti | 586 | T → L in AAB21125 (PubMed:1310149).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00696 Genomic DNA Translation: CAB43023.1 Sequence problems. V00696 Genomic DNA Translation: CAA24073.2 Sequence problems. KP263414 Genomic DNA Translation: AIZ98891.1 S76640 Genomic DNA Translation: AAB21125.2 |
PIRi | S78666, QQBY2M |
RefSeqi | NP_009316.1, NC_001224.1 |
Genome annotation databases
EnsemblFungii | Q0120_mRNA; Q0120; Q0120 |
GeneIDi | 854582 |
KEGGi | sce:Q0120 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00696 Genomic DNA Translation: CAB43023.1 Sequence problems. V00696 Genomic DNA Translation: CAA24073.2 Sequence problems. KP263414 Genomic DNA Translation: AIZ98891.1 S76640 Genomic DNA Translation: AAB21125.2 |
PIRi | S78666, QQBY2M |
RefSeqi | NP_009316.1, NC_001224.1 |
3D structure databases
SMRi | P03879 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34778, 8 interactors |
ComplexPortali | CPX-1314, bI4 intron splicing factor complex |
IntActi | P03879, 1 interactor |
STRINGi | 4932.Q0120 |
Proteomic databases
PaxDbi | P03879 |
PRIDEi | P03879 |
Genome annotation databases
EnsemblFungii | Q0120_mRNA; Q0120; Q0120 |
GeneIDi | 854582 |
KEGGi | sce:Q0120 |
Organism-specific databases
SGDi | S000007273, BI4 |
VEuPathDBi | FungiDB:Q0120 |
Phylogenomic databases
eggNOGi | KOG4663, Eukaryota |
GeneTreei | ENSGT00390000017948 |
HOGENOMi | CLU_429071_0_0_1 |
InParanoidi | P03879 |
Miscellaneous databases
PROi | PR:P03879 |
RNActi | P03879, protein |
Family and domain databases
CDDi | cd00284, Cytochrome_b_N, 1 hit |
Gene3Di | 1.20.810.10, 1 hit 3.10.28.10, 2 hits |
InterProi | View protein in InterPro IPR005797, Cyt_b/b6_N IPR027387, Cytb/b6-like_sf IPR016174, Di-haem_cyt_TM IPR027434, Homing_endonucl IPR004860, LAGLIDADG_2 |
Pfami | View protein in Pfam PF00033, Cytochrome_B, 1 hit PF00961, LAGLIDADG_1, 2 hits |
SUPFAMi | SSF55608, SSF55608, 2 hits SSF81342, SSF81342, 1 hit |
PROSITEi | View protein in PROSITE PS51002, CYTB_NTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MBI4_YEAST | |
Accessioni | P03879Primary (citable) accession number: P03879 Secondary accession number(s): A0A0A7NYH9, Q02324, Q9ZZW6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 19, 2004 | |
Last modified: | February 23, 2022 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - SIMILARITY comments
Index of protein domains and families