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Protein

Site-specific recombinase Flp

Gene

FLP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is >144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c.3 Publications

Miscellaneous

The plasmid 2-micron circle is a extrachromosomal element that resides in the nucleus and propagates itself stably in host cell populations. It provides no obvious advantage to the host but imposes no significant disadvantage either at its steady-state copy number of 40-60 molecules/cell.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei343O-(3'-phospho-DNA)-tyrosine intermediate1 Publication1

GO - Molecular functioni

  • DNA binding, bending Source: SGD
  • double-stranded DNA binding Source: SGD
  • single-stranded DNA binding Source: SGD
  • site-specific recombinase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA integration, DNA recombination

Names & Taxonomyi

Protein namesi
Recommended name:
Site-specific recombinase Flp
Short name:
FLP
Alternative name(s):
Protein Able
Gene namesi
Name:FLP1
Ordered Locus Names:R0010W
Encoded oniPlasmid 2-micron0 Publication
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Plasmid 2-micron

Organism-specific databases

SGDiS000029654 FLP1

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi305H → L or P: Inactive and weakened DNA binding. 1 Publication1
Mutagenesisi305H → Q: Reduced activity. 1 Publication1
Mutagenesisi308R → G: Inactive and weakened DNA binding. 1 Publication1
Mutagenesisi343Y → F or S: No strand cleavage or recombination. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001975671 – 423Site-specific recombinase FlpAdd BLAST423

Proteomic databases

PRIDEiP03870

Expressioni

Inductioni

Repressed by the negative regulatory complex REP1-REP2.1 Publication

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1423
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP03870
SMRiP03870
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03870

Family & Domainsi

Sequence similaritiesi

Belongs to the 'phage' integrase family.Curated

Phylogenomic databases

InParanoidiP03870
OrthoDBiEOG092C2Q6Y

Family and domain databases

Gene3Di1.10.443.10, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013762 Integrase-like_cat_sf
IPR005626 Recombinase_Flp_C
IPR022647 Recombinase_Flp_N
PfamiView protein in Pfam
PF05202 Flp_C, 1 hit
PF03930 Flp_N, 1 hit
SUPFAMiSSF56349 SSF56349, 1 hit

Sequencei

Sequence statusi: Complete.

P03870-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPQFGILCKT PPKVLVRQFV ERFERPSGEK IALCAAELTY LCWMITHNGT
60 70 80 90 100
AIKRATFMSY NTIISNSLSF DIVNKSLQFK YKTQKATILE ASLKKLIPAW
110 120 130 140 150
EFTIIPYYGQ KHQSDITDIV SSLQLQFESS EEADKGNSHS KKMLKALLSE
160 170 180 190 200
GESIWEITEK ILNSFEYTSR FTKTKTLYQF LFLATFINCG RFSDIKNVDP
210 220 230 240 250
KSFKLVQNKY LGVIIQCLVT ETKTSVSRHI YFFSARGRID PLVYLDEFLR
260 270 280 290 300
NSEPVLKRVN RTGNSSSNKQ EYQLLKDNLV RSYNKALKKN APYSIFAIKN
310 320 330 340 350
GPKSHIGRHL MTSFLSMKGL TELTNVVGNW SDKRASAVAR TTYTHQITAI
360 370 380 390 400
PDHYFALVSR YYAYDPISKE MIALKDETNP IEEWQHIEQL KGSAEGSIRY
410 420
PAWNGIISQE VLDYLSSYIN RRI
Length:423
Mass (Da):48,620
Last modified:July 21, 1986 - v1
Checksum:iB9C3DE95AB253BC3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5G → D AA sequence (PubMed:2995370).Curated1
Sequence conflicti5G → D AA sequence (PubMed:2040639).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01347 Genomic DNA Translation: AAB59340.1
PIRiA04502 PDBYA

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01347 Genomic DNA Translation: AAB59340.1
PIRiA04502 PDBYA

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FLOX-ray2.65A/B/C/D2-423[»]
1M6XX-ray2.80A/B/C/D1-423[»]
1P4EX-ray2.70A/B2-423[»]
C/D2-422[»]
ProteinModelPortaliP03870
SMRiP03870
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP03870

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

SGDiS000029654 FLP1

Phylogenomic databases

InParanoidiP03870
OrthoDBiEOG092C2Q6Y

Miscellaneous databases

EvolutionaryTraceiP03870
PROiPR:P03870

Family and domain databases

Gene3Di1.10.443.10, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013762 Integrase-like_cat_sf
IPR005626 Recombinase_Flp_C
IPR022647 Recombinase_Flp_N
PfamiView protein in Pfam
PF05202 Flp_C, 1 hit
PF03930 Flp_N, 1 hit
SUPFAMiSSF56349 SSF56349, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFLP_YEAST
AccessioniPrimary (citable) accession number: P03870
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 12, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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Main funding by: National Institutes of Health

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