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UniProtKB - P03856 (REPE1_ECOLI)
Protein
Replication initiation protein
Gene
repE
Organism
Escherichia coli (strain K12)
Status
Functioni
Replication initiator in the monomeric form, and autogenous repressor in the dimeric form.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 75 – 83 | H-T-H motif | 9 | |
DNA bindingi | 200 – 207 | H-T-H motif | 8 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: InterPro
GO - Biological processi
- DNA replication initiation Source: InterPro
- plasmid maintenance Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA replication, Plasmid copy control |
Names & Taxonomyi
Protein namesi | Recommended name: Replication initiation proteinShort name: Protein E Short name: Protein rep Alternative name(s): Protein F4 |
Gene namesi | Name:repE Synonyms:E, rep Ordered Locus Names:ECOK12F045 |
Encoded oni | Plasmid F |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068297 | 1 – 251 | Replication initiation proteinAdd BLAST | 251 |
Proteomic databases
PRIDEi | P03856 |
Interactioni
Subunit structurei
Monomer and homodimer.
1 PublicationProtein-protein interaction databases
DIPi | DIP-27645N |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P03856 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P03856 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 144 | DimerizationAdd BLAST | 144 |
Sequence similaritiesi
Belongs to the initiator RepB protein family.Curated
Phylogenomic databases
PhylomeDBi | P03856 |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
InterProi | View protein in InterPro IPR000525, Initiator_Rep_prot IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF01051, Rep_3, 1 hit |
SUPFAMi | SSF46785, SSF46785, 2 hits |
i Sequence
Sequence statusi: Complete.
P03856-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAETAVINHK KRKNSPRIVQ SNDLTEAAYS LSRDQKRMLY LFVDQIRKSD
60 70 80 90 100
GTLQEHDGIC EIHVAKYAEI FGLTSAEASK DIRQALKSFA GKEVVFYRPE
110 120 130 140 150
EDAGDEKGYE SFPWFIKRAH SPSRGLYSVH INPYLIPFFI GLQNRFTQFR
160 170 180 190 200
LSETKEITNP YAMRLYESLC QYRKPDGSGI VSLKIDWIIE RYQLPQSYQR
210 220 230 240 250
MPDFRRRFLQ VCVNEINSRT PMRLSYIEKK KGRQTTHIVF SFRDITSMTT
G
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01724 Genomic DNA Translation: AAA91062.1 M12987 Genomic DNA Translation: AAA24901.1 AP001918 Genomic DNA Translation: BAA97915.1 X03410 Genomic DNA Translation: CAA27146.1 X04619 Genomic DNA Translation: CAA28294.1 |
PIRi | A91487, QQECF |
RefSeqi | NP_061424.1, NC_002483.1 WP_000852146.1, NZ_STEB01000056.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01724 Genomic DNA Translation: AAA91062.1 M12987 Genomic DNA Translation: AAA24901.1 AP001918 Genomic DNA Translation: BAA97915.1 X03410 Genomic DNA Translation: CAA27146.1 X04619 Genomic DNA Translation: CAA28294.1 |
PIRi | A91487, QQECF |
RefSeqi | NP_061424.1, NC_002483.1 WP_000852146.1, NZ_STEB01000056.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1REP | X-ray | 2.60 | C | 1-251 | [»] | |
2Z9O | X-ray | 3.14 | A/B | 2-251 | [»] | |
7RVA | X-ray | 1.89 | C | 1-251 | [»] | |
SMRi | P03856 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-27645N |
Proteomic databases
PRIDEi | P03856 |
Phylogenomic databases
PhylomeDBi | P03856 |
Miscellaneous databases
EvolutionaryTracei | P03856 |
PROi | PR:P03856 |
Family and domain databases
Gene3Di | 1.10.10.10, 2 hits |
InterProi | View protein in InterPro IPR000525, Initiator_Rep_prot IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF01051, Rep_3, 1 hit |
SUPFAMi | SSF46785, SSF46785, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | REPE1_ECOLI | |
Accessioni | P03856Primary (citable) accession number: P03856 Secondary accession number(s): P08969 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, PlasmidDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families