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Protein

Peptidoglycan transglycosylase gp16

Gene

16

Organism
Enterobacteria phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cylindrical core that assembles on the inner surface of the capsid during capsid formation and plays a role in viral DNA ejection into the host cell. The inner core is composed of stacked rings of gp14, gp15 and gp16 proteins. Following binding to the host cell surface, the internal core is diassembled and gp16 is ejected along with gp14 and gp15 into the infected cell. Gp16 probably inserts in the host inner membrane and remains associated with gp15. The gp15-gp16 complex binds to both the viral DNA and the host inner membrane, probably escorting the leading end of the genome through the periplasm and controlling the extend of DNA translocated into the host cell. Functions as an exolysin that catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycans allowing the local digestion of the bacterial peptidoglycan wall.UniRule annotation4 Publications

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei37UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzyme, Hydrolase, Lyase, Viral short tail ejection system
Biological processDegradation of host cell envelope components during virus entry, Degradation of host peptidoglycans during virus entry, Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Virus entry into host cell

Protein family/group databases

CAZyiGH23 Glycoside Hydrolase Family 23
TCDBi3.A.17.1.1 the phage t7 injectisome (t7 injectisome) family

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan transglycosylase gp16UniRule annotation (EC:4.2.2.n1UniRule annotation1 Publication)
Alternative name(s):
Internal core protein gp16UniRule annotation
Gene namesi
Ordered Locus Names:16
OrganismiEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifieri10760 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000840 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 1136PeriplasmicUniRule annotation1 PublicationAdd BLAST1136
Transmembranei1137 – 1157HelicalUniRule annotationAdd BLAST21
Topological domaini1158 – 1318CytoplasmicUniRule annotation1 PublicationAdd BLAST161

GO - Cellular componenti

Keywords - Cellular componenti

Host cell inner membrane, Host cell membrane, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001965601 – 1318Peptidoglycan transglycosylase gp16Add BLAST1318

Proteomic databases

PRIDEiP03726

Interactioni

Subunit structurei

Homotetramer. Interacts with gp15; after ejection the gp15-gp16 complex composed of a gp15 octamer and a gp16 tetramer probably binds both the viral DNA and the host inner membrane.UniRule annotation2 Publications

Protein-protein interaction databases

IntActiP03726, 1 interactor
MINTiP03726

Structurei

3D structure databases

ProteinModelPortaliP03726
SMRiP03726
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 111Transglycosylase SLT-type domainUniRule annotationAdd BLAST88
Regioni1314 – 1318Essential for viral DNA translocationUniRule annotation1 Publication5

Domaini

The N-terminus contains the transglycosylase activity (Potential). The C-terminus is essential for the viral DNA translocation into the host cytoplasm.UniRule annotation1 Publication

Sequence similaritiesi

Belongs to the transglycosylase Slt family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK21508
OrthoDBiVOG0900000M

Family and domain databases

HAMAPiMF_04121 TRANSGLYCOSYLASE_T7, 1 hit
InterProiView protein in InterPro
IPR038994 Gp16
IPR023346 Lysozyme-like_dom_sf
IPR000189 Transglyc_AS
IPR008258 Transglycosylase_SLT_dom_1
PfamiView protein in Pfam
PF01464 SLT, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
PROSITEiView protein in PROSITE
PS00922 TRANSGLYCOSYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

P03726-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDKYDKNVPS DYDGLFQKAA DANGVSYDLL RKVAWTESRF VPTAKSKTGP
60 70 80 90 100
LGMMQFTKAT AKALGLRVTD GPDDDRLNPE LAINAAAKQL AGLVGKFDGD
110 120 130 140 150
ELKAALAYNQ GEGRLGNPQL EAYSKGDFAS ISEEGRNYMR NLLDVAKSPM
160 170 180 190 200
AGQLETFGGI TPKGKGIPAE VGLAGIGHKQ KVTQELPEST SFDVKGIEQE
210 220 230 240 250
ATAKPFAKDF WETHGETLDE YNSRSTFFGF KNAAEAELSN SVAGMAFRAG
260 270 280 290 300
RLDNGFDVFK DTITPTRWNS HIWTPEELEK IRTEVKNPAY INVVTGGSPE
310 320 330 340 350
NLDDLIKLAN ENFENDSRAA EAGLGAKLSA GIIGAGVDPL SYVPMVGVTG
360 370 380 390 400
KGFKLINKAL VVGAESAALN VASEGLRTSV AGGDADYAGA ALGGFVFGAG
410 420 430 440 450
MSAISDAVAA GLKRSKPEAE FDNEFIGPMM RLEARETARN ANSADLSRMN
460 470 480 490 500
TENMKFEGEH NGVPYEDLPT ERGAVVLHDG SVLSASNPIN PKTLKEFSEV
510 520 530 540 550
DPEKAARGIK LAGFTEIGLK TLGSDDADIR RVAIDLVRSP TGMQSGASGK
560 570 580 590 600
FGATASDIHE RLHGTDQRTY NDLYKAMSDA MKDPEFSTGG AKMSREETRY
610 620 630 640 650
TIYRRAALAI ERPELQKALT PSERIVMDII KRHFDTKREL MENPAIFGNT
660 670 680 690 700
KAVSIFPESR HKGTYVPHVY DRHAKALMIQ RYGAEGLQEG IARSWMNSYV
710 720 730 740 750
SRPEVKARVD EMLKELHGVK EVTPEMVEKY AMDKAYGISH SDQFTNSSII
760 770 780 790 800
EENIEGLVGI ENNSFLEARN LFDSDLSITM PDGQQFSVND LRDFDMFRIM
810 820 830 840 850
PAYDRRVNGD IAIMGSTGKT TKELKDEILA LKAKAEGDGK KTGEVHALMD
860 870 880 890 900
TVKILTGRAR RNQDTVWETS LRAINDLGFF AKNAYMGAQN ITEIAGMIVT
910 920 930 940 950
GNVRALGHGI PILRDTLYKS KPVSAKELKE LHASLFGKEV DQLIRPKRAD
960 970 980 990 1000
IVQRLREATD TGPAVANIVG TLKYSTQELA ARSPWTKLLN GTTNYLLDAA
1010 1020 1030 1040 1050
RQGMLGDVIS ATLTGKTTRW EKEGFLRGAS VTPEQMAGIK SLIKEHMVRG
1060 1070 1080 1090 1100
EDGKFTVKDK QAFSMDPRAM DLWRLADKVA DEAMLRPHKV SLQDSHAFGA
1110 1120 1130 1140 1150
LGKMVMQFKS FTIKSLNSKF LRTFYDGYKN NRAIDAALSI ITSMGLAGGF
1160 1170 1180 1190 1200
YAMAAHVKAY ALPKEKRKEY LERALDPTMI AHAALSRSSQ LGAPLAMVDL
1210 1220 1230 1240 1250
VGGVLGFESS KMARSTILPK DTVKERDPNK PYTSREVMGA MGSNLLEQMP
1260 1270 1280 1290 1300
SAGFVANVGA TLMNAAGVVN SPNKATEQDF MTGLMNSTKE LVPNDPLTQQ
1310
LVLKIYEANG VNLRERRK
Length:1,318
Mass (Da):143,838
Last modified:July 21, 1986 - v1
Checksum:i51A0AAA920CBF210
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA Translation: CAA24434.1
PIRiA04352 HIBPD7
RefSeqiNP_042004.1, NC_001604.1

Genome annotation databases

GeneIDi1261031
KEGGivg:1261031

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA Translation: CAA24434.1
PIRiA04352 HIBPD7
RefSeqiNP_042004.1, NC_001604.1

3D structure databases

ProteinModelPortaliP03726
SMRiP03726
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03726, 1 interactor
MINTiP03726

Protein family/group databases

CAZyiGH23 Glycoside Hydrolase Family 23
TCDBi3.A.17.1.1 the phage t7 injectisome (t7 injectisome) family

Proteomic databases

PRIDEiP03726

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261031
KEGGivg:1261031

Phylogenomic databases

KOiK21508
OrthoDBiVOG0900000M

Family and domain databases

HAMAPiMF_04121 TRANSGLYCOSYLASE_T7, 1 hit
InterProiView protein in InterPro
IPR038994 Gp16
IPR023346 Lysozyme-like_dom_sf
IPR000189 Transglyc_AS
IPR008258 Transglycosylase_SLT_dom_1
PfamiView protein in Pfam
PF01464 SLT, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
PROSITEiView protein in PROSITE
PS00922 TRANSGLYCOSYLASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEXLYS_BPT7
AccessioniPrimary (citable) accession number: P03726
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 10, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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