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Entry version 106 (02 Dec 2020)
Sequence version 1 (21 Jul 1986)
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Protein

Peptidoglycan transglycosylase gp16

Gene

16

Organism
Escherichia phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the cylindrical core that assembles on the inner surface of the capsid during capsid formation and plays a role in viral DNA ejection into the host cell. The inner core is composed of stacked rings of gp14, gp15 and gp16 proteins. Following binding to the host cell surface, the internal core is diassembled and gp16 is ejected along with gp14 and gp15 into the infected cell. Gp16 probably inserts in the host inner membrane and remains associated with gp15. The gp15-gp16 complex binds to both the viral DNA and the host inner membrane, probably escorting the leading end of the genome through the periplasm and controlling the extend of DNA translocated into the host cell. Functions as an exolysin that catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycans allowing the local digestion of the bacterial peptidoglycan wall.UniRule annotation4 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation1 Publication EC:4.2.2.n1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei37UniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzyme, Hydrolase, Lyase, Viral short tail ejection system
Biological processDegradation of host cell envelope components during virus entry, Degradation of host peptidoglycans during virus entry, Viral genome ejection through host cell envelope, Viral penetration into host cytoplasm, Virus entry into host cell

Protein family/group databases

Carbohydrate-Active enZymes

More...
CAZyi
GH23, Glycoside Hydrolase Family 23

Transport Classification Database

More...
TCDBi
3.A.17.1.1, the phage t7 injectisome (t7 injectisome) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Peptidoglycan transglycosylase gp16UniRule annotation (EC:4.2.2.n1UniRule annotation1 Publication)
Alternative name(s):
Internal core protein gp16UniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Ordered Locus Names:16
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia phage T7 (Bacteriophage T7)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10760 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesDuplodnaviriaHeunggongviraeUroviricotaCaudoviricetesCaudoviralesAutographiviridaeStudiervirinaeTeseptimavirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiEscherichia coli [TaxID: 562]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000840 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 1136PeriplasmicUniRule annotation1 PublicationAdd BLAST1136
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei1137 – 1157HelicalUniRule annotationAdd BLAST21
Topological domaini1158 – 1318CytoplasmicUniRule annotation1 PublicationAdd BLAST161

Keywords - Cellular componenti

Host cell inner membrane, Host cell membrane, Host membrane, Membrane, Virion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001965601 – 1318Peptidoglycan transglycosylase gp16Add BLAST1318

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homotetramer.

Interacts with gp15; after ejection the gp15-gp16 complex composed of a gp15 octamer and a gp16 tetramer probably binds both the viral DNA and the host inner membrane.

UniRule annotation2 Publications

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
P03726, 1 interactor

Molecular INTeraction database

More...
MINTi
P03726

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P03726

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni24 – 111Transglycosylase SLT-type domainUniRule annotationAdd BLAST88
Regioni1314 – 1318Essential for viral DNA translocationUniRule annotation1 Publication5

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The N-terminus contains the transglycosylase activity (Potential). The C-terminus is essential for the viral DNA translocation into the host cytoplasm.UniRule annotation1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the transglycosylase Slt family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

HAMAP database of protein families

More...
HAMAPi
MF_04121, TRANSGLYCOSYLASE_T7, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR038994, Gp16
IPR023346, Lysozyme-like_dom_sf
IPR000189, Transglyc_AS
IPR008258, Transglycosylase_SLT_dom_1

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01464, SLT, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53955, SSF53955, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00922, TRANSGLYCOSYLASE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P03726-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDKYDKNVPS DYDGLFQKAA DANGVSYDLL RKVAWTESRF VPTAKSKTGP
60 70 80 90 100
LGMMQFTKAT AKALGLRVTD GPDDDRLNPE LAINAAAKQL AGLVGKFDGD
110 120 130 140 150
ELKAALAYNQ GEGRLGNPQL EAYSKGDFAS ISEEGRNYMR NLLDVAKSPM
160 170 180 190 200
AGQLETFGGI TPKGKGIPAE VGLAGIGHKQ KVTQELPEST SFDVKGIEQE
210 220 230 240 250
ATAKPFAKDF WETHGETLDE YNSRSTFFGF KNAAEAELSN SVAGMAFRAG
260 270 280 290 300
RLDNGFDVFK DTITPTRWNS HIWTPEELEK IRTEVKNPAY INVVTGGSPE
310 320 330 340 350
NLDDLIKLAN ENFENDSRAA EAGLGAKLSA GIIGAGVDPL SYVPMVGVTG
360 370 380 390 400
KGFKLINKAL VVGAESAALN VASEGLRTSV AGGDADYAGA ALGGFVFGAG
410 420 430 440 450
MSAISDAVAA GLKRSKPEAE FDNEFIGPMM RLEARETARN ANSADLSRMN
460 470 480 490 500
TENMKFEGEH NGVPYEDLPT ERGAVVLHDG SVLSASNPIN PKTLKEFSEV
510 520 530 540 550
DPEKAARGIK LAGFTEIGLK TLGSDDADIR RVAIDLVRSP TGMQSGASGK
560 570 580 590 600
FGATASDIHE RLHGTDQRTY NDLYKAMSDA MKDPEFSTGG AKMSREETRY
610 620 630 640 650
TIYRRAALAI ERPELQKALT PSERIVMDII KRHFDTKREL MENPAIFGNT
660 670 680 690 700
KAVSIFPESR HKGTYVPHVY DRHAKALMIQ RYGAEGLQEG IARSWMNSYV
710 720 730 740 750
SRPEVKARVD EMLKELHGVK EVTPEMVEKY AMDKAYGISH SDQFTNSSII
760 770 780 790 800
EENIEGLVGI ENNSFLEARN LFDSDLSITM PDGQQFSVND LRDFDMFRIM
810 820 830 840 850
PAYDRRVNGD IAIMGSTGKT TKELKDEILA LKAKAEGDGK KTGEVHALMD
860 870 880 890 900
TVKILTGRAR RNQDTVWETS LRAINDLGFF AKNAYMGAQN ITEIAGMIVT
910 920 930 940 950
GNVRALGHGI PILRDTLYKS KPVSAKELKE LHASLFGKEV DQLIRPKRAD
960 970 980 990 1000
IVQRLREATD TGPAVANIVG TLKYSTQELA ARSPWTKLLN GTTNYLLDAA
1010 1020 1030 1040 1050
RQGMLGDVIS ATLTGKTTRW EKEGFLRGAS VTPEQMAGIK SLIKEHMVRG
1060 1070 1080 1090 1100
EDGKFTVKDK QAFSMDPRAM DLWRLADKVA DEAMLRPHKV SLQDSHAFGA
1110 1120 1130 1140 1150
LGKMVMQFKS FTIKSLNSKF LRTFYDGYKN NRAIDAALSI ITSMGLAGGF
1160 1170 1180 1190 1200
YAMAAHVKAY ALPKEKRKEY LERALDPTMI AHAALSRSSQ LGAPLAMVDL
1210 1220 1230 1240 1250
VGGVLGFESS KMARSTILPK DTVKERDPNK PYTSREVMGA MGSNLLEQMP
1260 1270 1280 1290 1300
SAGFVANVGA TLMNAAGVVN SPNKATEQDF MTGLMNSTKE LVPNDPLTQQ
1310
LVLKIYEANG VNLRERRK
Length:1,318
Mass (Da):143,838
Last modified:July 21, 1986 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i51A0AAA920CBF210
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
V01146 Genomic DNA Translation: CAA24434.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A04352, HIBPD7

NCBI Reference Sequences

More...
RefSeqi
NP_042004.1, NC_001604.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1261031

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
vg:1261031

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA Translation: CAA24434.1
PIRiA04352, HIBPD7
RefSeqiNP_042004.1, NC_001604.1

3D structure databases

SMRiP03726
ModBaseiSearch...

Protein-protein interaction databases

IntActiP03726, 1 interactor
MINTiP03726

Protein family/group databases

CAZyiGH23, Glycoside Hydrolase Family 23
TCDBi3.A.17.1.1, the phage t7 injectisome (t7 injectisome) family

Genome annotation databases

GeneIDi1261031
KEGGivg:1261031

Family and domain databases

HAMAPiMF_04121, TRANSGLYCOSYLASE_T7, 1 hit
InterProiView protein in InterPro
IPR038994, Gp16
IPR023346, Lysozyme-like_dom_sf
IPR000189, Transglyc_AS
IPR008258, Transglycosylase_SLT_dom_1
PfamiView protein in Pfam
PF01464, SLT, 1 hit
SUPFAMiSSF53955, SSF53955, 1 hit
PROSITEiView protein in PROSITE
PS00922, TRANSGLYCOSYLASE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiEXLYS_BPT7
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P03726
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: December 2, 2020
This is version 106 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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