R - Endolysin - Escherichia phage lambda

Functionⁱ

Endolysin with transglycosylase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.UniRule annotation2 Publications

Catalytic activityⁱ

Endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation2 Publications

Activity regulationⁱ

Inactivated by zinc.1 Publication

pH dependenceⁱ

Optimum pH is 6.6.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	19	UniRule annotation1 Publication		1

GO - Molecular functionⁱ

lyase activity Source: UniProtKB-KW
lysozyme activity
Source: UniProtKB
Inferred from direct assayⁱ
- 4889461
lytic transglycosylase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 9514719

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell wall macromolecule catabolic process Source: InterPro
cytolysis Source: UniProtKB-KW
cytolysis by virus of host cell
Source: UniProtKB
Inferred from direct assayⁱ
- 6448076
defense response to bacterium Source: UniProtKB-KW
peptidoglycan catabolic process Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Antimicrobial, Bacteriolytic enzyme, Lyase
Biological process	Cytolysis, Host cell lysis by virus, Viral release from host cell

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GH104 Glycoside Hydrolase Family 104

CAZyⁱ

GH104 Glycoside Hydrolase Family 104

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Endolysin1 PublicationUniRule annotation (EC:4.2.2.n2UniRule annotation2 Publications) Alternative name(s): Lysis proteinUniRule annotation Lysozyme2 PublicationsUniRule annotation Transglycosylase1 PublicationUniRule annotation
Gene namesⁱ	Name:R
Organismⁱ	Escherichia phage lambda (Bacteriophage lambda)
Taxonomic identifierⁱ	10710 [NCBI]
Taxonomic lineageⁱ	Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambdavirus
Virus hostⁱ	Escherichia coli [TaxID: 562]
Proteomesⁱ	UP000001711 Componentⁱ: Genome

Subcellular locationⁱ

Host cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_04109

Note:

The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.UniRule annotation1 Publication

GO - Cellular componentⁱ

host cell cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	31	H → D: No effect on lytic activity. 1 Publication	1
Mutagenesisⁱ	31	H → N: No effect on lytic activity; when associated with N-137. 1 Publication	1
Mutagenesisⁱ	48	H → N: 97% loss of lytic activity. 1 Publication	1
Mutagenesisⁱ	137	H → N: No effect on lytic activity; when associated with N-31. 1 Publication	1

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB02325 Isopropyl Alcohol DB04206 Nz2-Tryptophan

DrugBankⁱ

DB02325 Isopropyl Alcohol
DB04206 Nz2-Tryptophan

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000218108	1 – 158	EndolysinAdd BLAST		158

Interactionⁱ

Subunit structureⁱ

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

IntActⁱ	P03706, 1 interactor

IntActⁱ

P03706, 1 interactor

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	6 – 16	Combined sources	11
Beta strandⁱ	20 – 26	Combined sources	7
Turnⁱ	31 – 34	Combined sources	4
Beta strandⁱ	53 – 56	Combined sources	4
Beta strandⁱ	59 – 61	Combined sources	3
Turnⁱ	65 – 68	Combined sources	4
Helixⁱ	76 – 81	Combined sources	6
Helixⁱ	88 – 101	Combined sources	14
Helixⁱ	105 – 110	Combined sources	6
Helixⁱ	113 – 120	Combined sources	8
Turnⁱ	121 – 123	Combined sources	3
Beta strandⁱ	132 – 134	Combined sources	3
Helixⁱ	135 – 148	Combined sources	14

Show more details

3D structure databases

ProteinModelPortalⁱ	P03706
SMRⁱ	P03706
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P03706

EvolutionaryTraceⁱ

P03706

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the glycosyl hydrolase 24 family.UniRule annotation

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG090000W9

OrthoDBⁱ

VOG090000W9

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00736 bacteriophage_lambda_lysozyme, 1 hit
HAMAPⁱ	MF_04109 ENDOLYSIN_LAMBDA, 1 hit
InterProⁱ	View protein in InterPro IPR034691 Endolysin_lambda_type IPR002196 Glyco_hydro_24 IPR023346 Lysozyme-like_dom_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00959 Phage_lysozyme, 1 hit
SUPFAMⁱ	SSF53955 SSF53955, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P03706-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVEINNQRKA FLDMLAWSEG TDNGRQKTRN HGYDVIVGGE LFTDYSDHPR 
        60         70         80         90        100
KLVTLNPKLK STGAGRYQLL SRWWDAYRKQ LGLKDFSPKS QDAVALQQIK 
       110        120        130        140        150
ERGALPMIDR GDIRQAIDRC SNIWASLPGA GYGQFEHKAD SLIAKFKEAG 

GTVREIDV

Length:158

Mass (Da):17,825

Last modified:July 21, 1986 - v1

Checksum:ⁱ94F8E9A512EAA03E

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	74	Missing AA sequence (Ref. 2) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J02459 Genomic DNA Translation: AAA96598.1
PIRⁱ	B04333 EYBPL
NCBI Reference Sequences More...RefSeqⁱ	NP_040645.1, NC_001416.1

Genome annotation databases

GeneIDⁱ	2703480
KEGGⁱ	vg:2703480

Protein	Similar proteins		Species	Score	Length	Source
P03706	Endolysin		Enterobacteria phage DE3		158	UniRef100_P03706
Endolysin		Enterobacteria phage HK630		158
Endolysin		LAMBD		158
Lysozyme		STREE		158
Lysozyme		Andreesenia angusta		158
+12

Protein	Similar proteins		Species	Score	Length	Source
P03706	Endolysin		Enterobacteria phage DE3		158	UniRef90_P03706
Endolysin		Enterobacteria phage HK630		158
Endolysin		LAMBD		158
Lysozyme		STREE		158
Lysozyme		Andreesenia angusta		158
+668

Protein	Similar proteins		Species	Score	Length	Source
P03706	Endolysin		Enterobacteria phage DE3		158	UniRef50_P03706
Endolysin		Enterobacteria phage HK630		158
Endolysin		LAMBD		158
Lysozyme		STREE		158
Lysozyme		Andreesenia angusta		158
+1555

Entry informationⁱ

Entry nameⁱ	ENLYS_LAMBD
Accessionⁱ	P03706Primary (citable) accession number: P03706
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	July 21, 1986
	Last modified:	September 12, 2018
	This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

UniProtKB

UniParc

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UniRef

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Annotation systems

Supporting data

UniProtKB - P03706 (ENLYS_LAMBD)

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Endolysin

R

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

pH dependenceⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ