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Protein

Endolysin

Gene

R

Organism
Escherichia phage lambda (Bacteriophage lambda)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endolysin with transglycosylase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.UniRule annotation2 Publications

Catalytic activityi

Endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation2 Publications

Activity regulationi

Inactivated by zinc.1 Publication

pH dependencei

Optimum pH is 6.6.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei19UniRule annotation1 Publication1

GO - Molecular functioni

  • lyase activity Source: UniProtKB-KW
  • lysozyme activity Source: UniProtKB
  • lytic transglycosylase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzyme, Lyase
Biological processCytolysis, Host cell lysis by virus, Viral release from host cell

Protein family/group databases

CAZyiGH104 Glycoside Hydrolase Family 104

Names & Taxonomyi

Protein namesi
Recommended name:
Endolysin1 PublicationUniRule annotation (EC:4.2.2.n2UniRule annotation2 Publications)
Alternative name(s):
Lysis proteinUniRule annotation
Lysozyme2 PublicationsUniRule annotation
Transglycosylase1 PublicationUniRule annotation
Gene namesi
Name:R
OrganismiEscherichia phage lambda (Bacteriophage lambda)
Taxonomic identifieri10710 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdavirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000001711 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31H → D: No effect on lytic activity. 1 Publication1
Mutagenesisi31H → N: No effect on lytic activity; when associated with N-137. 1 Publication1
Mutagenesisi48H → N: 97% loss of lytic activity. 1 Publication1
Mutagenesisi137H → N: No effect on lytic activity; when associated with N-31. 1 Publication1

Chemistry databases

DrugBankiDB02325 Isopropyl Alcohol
DB04206 Nz2-Tryptophan

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002181081 – 158EndolysinAdd BLAST158

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

IntActiP03706, 1 interactor

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP03706
SMRiP03706
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03706

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000W9

Family and domain databases

CDDicd00736 bacteriophage_lambda_lysozyme, 1 hit
HAMAPiMF_04109 ENDOLYSIN_LAMBDA, 1 hit
InterProiView protein in InterPro
IPR034691 Endolysin_lambda_type
IPR002196 Glyco_hydro_24
IPR023346 Lysozyme-like_dom_sf
PfamiView protein in Pfam
PF00959 Phage_lysozyme, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit

Sequencei

Sequence statusi: Complete.

P03706-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVEINNQRKA FLDMLAWSEG TDNGRQKTRN HGYDVIVGGE LFTDYSDHPR
60 70 80 90 100
KLVTLNPKLK STGAGRYQLL SRWWDAYRKQ LGLKDFSPKS QDAVALQQIK
110 120 130 140 150
ERGALPMIDR GDIRQAIDRC SNIWASLPGA GYGQFEHKAD SLIAKFKEAG

GTVREIDV
Length:158
Mass (Da):17,825
Last modified:July 21, 1986 - v1
Checksum:i94F8E9A512EAA03E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74Missing AA sequence (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02459 Genomic DNA Translation: AAA96598.1
PIRiB04333 EYBPL
RefSeqiNP_040645.1, NC_001416.1

Genome annotation databases

GeneIDi2703480
KEGGivg:2703480

Similar proteinsi

Entry informationi

Entry nameiENLYS_LAMBD
AccessioniPrimary (citable) accession number: P03706
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 12, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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