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UniProtKB - P03706 (ENLYS_LAMBD)
Protein
Endolysin
Gene
R
Organism
Escherichia phage lambda (Bacteriophage lambda)
Status
Functioni
Endolysin with transglycosylase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.
UniRule annotation2 PublicationsCatalytic activityi
- Endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation2 Publications EC:4.2.2.n2
Activity regulationi
Inactivated by zinc.1 Publication
pH dependencei
Optimum pH is 6.6.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 19 | UniRule annotation1 Publication | 1 |
GO - Molecular functioni
- lyase activity Source: UniProtKB-UniRule
- lysozyme activity Source: UniProtKB
- lytic transglycosylase activity Source: UniProtKB
GO - Biological processi
- cell wall macromolecule catabolic process Source: InterPro
- cytolysis Source: UniProtKB-KW
- defense response to bacterium Source: UniProtKB-KW
- peptidoglycan catabolic process Source: UniProtKB-UniRule
- viral release from host cell by cytolysis Source: UniProtKB
Keywordsi
Molecular function | Antimicrobial, Bacteriolytic enzyme, Lyase |
Biological process | Cytolysis, Host cell lysis by virus, Viral release from host cell |
Protein family/group databases
CAZyi | GH104, Glycoside Hydrolase Family 104 |
Names & Taxonomyi
Protein namesi | Recommended name: Endolysin1 PublicationUniRule annotation (EC:4.2.2.n2UniRule annotation2 Publications)Alternative name(s): Lysis proteinUniRule annotation Lysozyme2 PublicationsUniRule annotation Transglycosylase1 PublicationUniRule annotation |
Gene namesi | Name:R |
Organismi | Escherichia phage lambda (Bacteriophage lambda) |
Taxonomic identifieri | 10710 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Uroviricota › Caudoviricetes › Caudovirales › Siphoviridae › Lambdavirus |
Virus hosti | Escherichia coli [TaxID: 562] |
Proteomesi |
|
Subcellular locationi
- Host cytoplasm UniRule annotation Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.UniRule annotation1 Publication
Keywords - Cellular componenti
Host cytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 31 | H → D: No effect on lytic activity. 1 Publication | 1 | |
Mutagenesisi | 31 | H → N: No effect on lytic activity; when associated with N-137. 1 Publication | 1 | |
Mutagenesisi | 48 | H → N: 97% loss of lytic activity. 1 Publication | 1 | |
Mutagenesisi | 137 | H → N: No effect on lytic activity; when associated with N-31. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB04206, 7-Aza-L-tryptophan |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218108 | 1 – 158 | EndolysinAdd BLAST | 158 |
Interactioni
Subunit structurei
Monomer.
UniRule annotation1 PublicationProtein-protein interaction databases
IntActi | P03706, 1 interactor |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P03706 |
SMRi | P03706 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P03706 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 24 family.UniRule annotation
Family and domain databases
HAMAPi | MF_04109, ENDOLYSIN_LAMBDA, 1 hit |
InterProi | View protein in InterPro IPR034691, Endolysin_lambda_type IPR002196, Glyco_hydro_24 IPR023346, Lysozyme-like_dom_sf |
Pfami | View protein in Pfam PF00959, Phage_lysozyme, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit |
i Sequence
Sequence statusi: Complete.
P03706-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVEINNQRKA FLDMLAWSEG TDNGRQKTRN HGYDVIVGGE LFTDYSDHPR
60 70 80 90 100
KLVTLNPKLK STGAGRYQLL SRWWDAYRKQ LGLKDFSPKS QDAVALQQIK
110 120 130 140 150
ERGALPMIDR GDIRQAIDRC SNIWASLPGA GYGQFEHKAD SLIAKFKEAG
GTVREIDV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 74 | Missing AA sequence (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02459 Genomic DNA Translation: AAA96598.1 |
PIRi | B04333, EYBPL |
RefSeqi | NP_040645.1, NC_001416.1 |
Genome annotation databases
GeneIDi | 2703480 |
KEGGi | vg:2703480 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02459 Genomic DNA Translation: AAA96598.1 |
PIRi | B04333, EYBPL |
RefSeqi | NP_040645.1, NC_001416.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AM7 | X-ray | 2.30 | A/B/C | 1-158 | [»] | |
1D9U | X-ray | 2.60 | A/B | 1-154 | [»] | |
3D3D | X-ray | 2.60 | A/B | 1-154 | [»] | |
BMRBi | P03706 | |||||
SMRi | P03706 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P03706, 1 interactor |
Chemistry databases
DrugBanki | DB04206, 7-Aza-L-tryptophan |
Protein family/group databases
CAZyi | GH104, Glycoside Hydrolase Family 104 |
Genome annotation databases
GeneIDi | 2703480 |
KEGGi | vg:2703480 |
Miscellaneous databases
EvolutionaryTracei | P03706 |
Family and domain databases
HAMAPi | MF_04109, ENDOLYSIN_LAMBDA, 1 hit |
InterProi | View protein in InterPro IPR034691, Endolysin_lambda_type IPR002196, Glyco_hydro_24 IPR023346, Lysozyme-like_dom_sf |
Pfami | View protein in Pfam PF00959, Phage_lysozyme, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ENLYS_LAMBD | |
Accessioni | P03706Primary (citable) accession number: P03706 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | May 25, 2022 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families