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Protein

Internal scaffolding protein B

Gene

B

Organism
Enterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the assembly of the viral procapsid in the cytoplasm. Forms first a 12S pre-assembly complex with protein H, and F and G pentamers, then twelve 12S complexes are joined by the D protein to form the procapsid. Internal scaffold protein B is released from the procapsid upon genome packaging (PubMed:159449). Autoproteolytic activity cleaves protein B and probably facilitates its removal through the pores of the procapsid (PubMed:12473449).2 Publications

GO - Molecular functioni

GO - Biological processi

  • viral scaffold assembly and maintenance Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease
Biological processViral capsid assembly, Viral release from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Internal scaffolding protein B (EC:3.4.-.-1 Publication)
Alternative name(s):
Scaffolding protein B
Short name:
GPB
Gene namesi
Name:B
OrganismiEnterobacteria phage phiX174 (Isolate Sanger) (Bacteriophage phi-X174)
Taxonomic identifieri1217068 [NCBI]
Taxonomic lineageiVirusesssDNA virusesMicroviridaeBullavirinaePhix174microvirus
Virus hostiEscherichia coli C [TaxID: 498388]
Proteomesi
  • UP000005893 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • host cell cytoplasm Source: UniProtKB-SubCell
  • viral procapsid Source: InterPro
  • viral scaffold Source: UniProtKB

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001648701 – 120Internal scaffolding protein BAdd BLAST120

Post-translational modificationi

The proteolytic cleavage of the internal scaffolding protein B releases the scaffold protein in order to continue virion assembly.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei77 – 78Cleavage; by autolysis1 Publication2
Sitei93 – 94Cleavage; by autolysis1 Publication2
Sitei108 – 109Cleavage; by autolysis1 Publication2

Keywords - PTMi

Autocatalytic cleavage

Interactioni

Subunit structurei

Component of the procapsid complex composed of 60 copies of the internally located B, 240 copies of the external scaffolding protein D, 60 copies of each of the viral structural proteins F and G proteins, and 12 copies of H.1 Publication

Structurei

Secondary structure

1120
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi62 – 79Combined sources18
Helixi87 – 90Combined sources4
Helixi112 – 115Combined sources4

3D structure databases

ProteinModelPortaliP03633
SMRiP03633
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03633

Family & Domainsi

Sequence similaritiesi

Belongs to the microviridae B protein family.Curated

Phylogenomic databases

OrthoDBiVOG0900015X

Family and domain databases

Gene3Di4.10.1260.10, 1 hit
InterProiView protein in InterPro
IPR003513 Phage_B
IPR038149 Phage_B_sf
PfamiView protein in Pfam
PF02304 Phage_B, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012751 Phage_B, 1 hit

Sequencei

Sequence statusi: Complete.

P03633-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQLTKNQAV ATSQEAVQNQ NEPQLRDENA HNDKSVHGVL NPTYQAGLRR
60 70 80 90 100
DAVQPDIEAE RKKRDEIEAG KSYCSRRFGG ATCDDKSAQI YARFDKNDWR
110 120
IQPAEFYRFH DAEVNTFGYF
Length:120
Mass (Da):13,843
Last modified:July 21, 1986 - v1
Checksum:iD8E52894116497ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02482 Genomic DNA Translation: AAA32572.1
PIRiA04241 ZBBPF4

Similar proteinsi

Entry informationi

Entry nameiSCAFB_BPPHS
AccessioniPrimary (citable) accession number: P03633
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 18, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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