UniProtKB - P03502 (NS1_INBLE)
Non-structural protein 1
NS
Functioni
Binds and inhibits the conjugation of the ubiquitin-like G1P2/ISG15 protein to its target proteins. Since G1P2/ISG15 is an early antiviral protein, NS1 may inhibit the host antiviral response. Prevents EIF2AK2/PKR activation, either by binding double strand RNA or by interacting directly with EIF2AK2/PKR. Also binds poly(A) and U6 snRNA.
UniRule annotation1 PublicationGO - Molecular functioni
- protein serine/threonine kinase inhibitor activity Source: UniProtKB-KW
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- suppression by virus of host ISG15-protein conjugation Source: UniProtKB-KW
- suppression by virus of host PKR signaling Source: UniProtKB-KW
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Non-structural protein 1UniRule annotationShort name: NS1UniRule annotation Alternative name(s): NS1AUniRule annotation |
Gene namesi | Name:NSUniRule annotation |
Organismi | Influenza B virus (strain B/Lee/1940) |
Taxonomic identifieri | 518987 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Polyploviricotina › Insthoviricetes › Articulavirales › Orthomyxoviridae › Betainfluenzavirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Host cytoplasm UniRule annotation
- Host nucleus UniRule annotation
Keywords - Cellular componenti
Host cytoplasm, Host nucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 33 | R → A: Partial loss of dsRNA-binding and no effect on inhibition of IFN-beta promoter; when associated with A-38. 1 Publication | 1 | |
Mutagenesisi | 38 | R → A: Partial loss of dsRNA-binding and no effect on inhibition of IFN-beta promoter; when associated with A-33. 1 Publication | 1 | |
Mutagenesisi | 47 | R → A: Complete loss of dsRNA-binding and 40% loss of inhibition of IFN-beta promoter; when associated with A-50. 1 Publication | 1 | |
Mutagenesisi | 50 | R → A: Complete loss of dsRNA-binding and 40% loss of inhibition of IFN-beta promoter; when associated with A-47. 1 Publication | 1 | |
Mutagenesisi | 52 | K → A: Partial loss of dsRNA-binding and 15% loss of inhibition of IFN-beta promoter; when associated with A-53 and A-54. 1 Publication | 1 | |
Mutagenesisi | 53 | R → A: Partial loss of dsRNA-binding and 15% loss of inhibition of IFN-beta promoter; when associated with A-52 and A-54. 1 Publication | 1 | |
Mutagenesisi | 54 | K → A: Partial loss of dsRNA-binding and 15% loss of inhibition of IFN-beta promoter; when associated with A-52 and A-53. 1 Publication | 1 | |
Mutagenesisi | 58 | R → A: Complete loss of dsRNA-binding and 20% loss of inhibition of IFN-beta promoter; when associated with A-60 and A-64. 1 Publication | 1 | |
Mutagenesisi | 60 | K → A: Complete loss of dsRNA-binding and 20% loss of inhibition of IFN-beta promoter; when associated with A-58 and A-64. 1 Publication | 1 | |
Mutagenesisi | 64 | K → A: Complete loss of dsRNA-binding and 20% loss of inhibition of IFN-beta promoter; when associated with A-58 and A-60. 1 Publication | 1 | |
Mutagenesisi | 70 | K → A: No effect on dsRNA-binding and inhibition of IFN-beta promoter; when associated with A-71. 1 Publication | 1 | |
Mutagenesisi | 71 | R → A: No effect on dsRNA-binding and inhibition of IFN-beta promoter; when associated with A-70. 1 Publication | 1 | |
Mutagenesisi | 77 | R → A: Complete loss of dsRNA-binding and inhibition of IFN-beta promoter; when associated with A-78. 1 Publication | 1 | |
Mutagenesisi | 78 | K → A: Complete loss of dsRNA-binding and inhibition of IFN-beta promoter; when associated with A-77. 1 Publication | 1 | |
Mutagenesisi | 83 | K → A: No effect on dsRNA-binding and 45% loss of inhibition of IFN-beta promoter; when associated with A-86. 1 Publication | 1 | |
Mutagenesisi | 86 | K → A: No effect on dsRNA-binding and 45% loss of inhibition of IFN-beta promoter; when associated with A-83. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000078960 | 1 – 281 | Non-structural protein 1Add BLAST | 281 |
Interactioni
Subunit structurei
Homodimer.
Interacts with and inhibits human G1P2 conjugation by UBE1L.
UniRule annotation1 PublicationBinary interactionsi
P03502
With | #Exp. | IntAct |
---|---|---|
ISG15 [P05161] from Homo sapiens. | 4 | EBI-15938710,EBI-746466 |
Protein-protein interaction databases
DIPi | DIP-59701N |
IntActi | P03502, 1 interactor |
Structurei
Secondary structure
3D structure databases
BMRBi | P03502 |
SMRi | P03502 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P03502 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 103 | G1P2-bindingAdd BLAST | 103 | |
Regioni | 1 – 93 | RNA-binding and homodimerizationUniRule annotationAdd BLAST | 93 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 50 – 55 | Nuclear localization signalUniRule annotation | 6 |
Domaini
Sequence similaritiesi
Family and domain databases
HAMAPi | MF_04066, INFV_NS1, 1 hit |
InterProi | View protein in InterPro IPR004208, NS1 IPR009068, S15_NS1_RNA-bd |
Pfami | View protein in Pfam PF02942, Flu_B_NS1, 1 hit |
PIRSFi | PIRSF003938, Flu_B_NS1, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADNMTTTQI EVGPGATNAT INFEAGILEC YERFSWQRAL DYPGQDRLHR
60 70 80 90 100
LKRKLESRIK THNKSEPENK RMSLEERKAI GVKMMKVLLF MDPSAGIEGF
110 120 130 140 150
EPYCVKNPST SKCPNYDWTD YPPTPGKYLD DIEEEPENVD HPIEVVLRDM
160 170 180 190 200
NNKDARQKIK DEVNTQKEGK FRLTIKRDIR NVLSLRVLVN GTFLKHPNGD
210 220 230 240 250
KSLSTLHRLN AYDQNGGLVA KLVATDDRTV EDEKDGHRIL NSLFERFDEG
260 270 280
HSKPIRAAET AVGVLSQFGQ EHRLSPEEGD N
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02096 Genomic RNA Translation: AAA43756.1 |
PIRi | A04093, MNIVA |
RefSeqi | NP_056666.1, NC_002211.1 [P03502-1] |
Genome annotation databases
GeneIDi | 26824008 |
KEGGi | vg:26824008 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02096 Genomic RNA Translation: AAA43756.1 |
PIRi | A04093, MNIVA |
RefSeqi | NP_056666.1, NC_002211.1 [P03502-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1XEQ | X-ray | 2.10 | A/B | 1-103 | [»] | |
3R66 | X-ray | 2.30 | A/B | 1-103 | [»] | |
3RT3 | X-ray | 2.01 | C | 1-103 | [»] | |
3SDL | X-ray | 2.29 | A/B | 1-103 | [»] | |
6J62 | X-ray | 2.49 | C | 1-103 | [»] | |
6JH0 | X-ray | 2.40 | A/C | 1-103 | [»] | |
6JH1 | X-ray | 3.00 | A/B | 1-103 | [»] | |
BMRBi | P03502 | |||||
SMRi | P03502 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-59701N |
IntActi | P03502, 1 interactor |
Protocols and materials databases
DNASUi | 26824008 |
Genome annotation databases
GeneIDi | 26824008 |
KEGGi | vg:26824008 |
Miscellaneous databases
EvolutionaryTracei | P03502 |
Family and domain databases
HAMAPi | MF_04066, INFV_NS1, 1 hit |
InterProi | View protein in InterPro IPR004208, NS1 IPR009068, S15_NS1_RNA-bd |
Pfami | View protein in Pfam PF02942, Flu_B_NS1, 1 hit |
PIRSFi | PIRSF003938, Flu_B_NS1, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NS1_INBLE | |
Accessioni | P03502Primary (citable) accession number: P03502 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families