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Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.UniRule annotation1 Publication1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological processEukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication, Viral transcription
LigandNucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-168255 Influenza Life Cycle
R-HSA-168271 Transport of Ribonucleoproteins into the Host Nucleus
R-HSA-168275 Entry of Influenza Virion into Host Cell via Endocytosis
R-HSA-168288 Fusion of the Influenza Virion to the Host Cell Endosome
R-HSA-168298 Release
R-HSA-168302 Budding
R-HSA-168303 Packaging of Eight RNA Segments
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-168330 Viral RNP Complexes in the Host Cell Nucleus
R-HSA-168333 NEP/NS2 Interacts with the Cellular Export Machinery
R-HSA-168336 Uncoating of the Influenza Virion
R-HSA-192814 vRNA Synthesis
R-HSA-192823 Viral mRNA Translation
R-HSA-192869 cRNA Synthesis
R-HSA-192905 vRNP Assembly

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase catalytic subunitUniRule annotation (EC:2.7.7.48UniRule annotation)
Alternative name(s):
Polymerase basic protein 1UniRule annotation
Short name:
PB1UniRule annotation
RNA-directed RNA polymerase subunit P1UniRule annotation
Gene namesi
Name:PB1UniRule annotation
OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Taxonomic identifieri211044 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeAlphainfluenzavirus
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000116373 Componenti: Genome
  • UP000170967 Componenti: Genome
  • UP000009255 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000787631 – 757RNA-directed RNA polymerase catalytic subunitAdd BLAST757

Post-translational modificationi

Phosphorylated by host PRKCA.UniRule annotation1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP03431

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.UniRule annotation2 Publications

Binary interactionsi

Protein-protein interaction databases

DIPiDIP-56974N
IntActiP03431, 141 interactors
MINTiP03431

Structurei

Secondary structure

1757
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP03431
SMRiP03431
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03431

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini286 – 483RdRp catalyticUniRule annotationAdd BLAST198

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni249 – 256Promoter-binding siteUniRule annotation8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi187 – 195Nuclear localization signalUniRule annotation9
Motifi203 – 216Nuclear localization signalUniRule annotationAdd BLAST14

Sequence similaritiesi

Belongs to the influenza viruses polymerase PB1 family.UniRule annotation

Phylogenomic databases

KOiK19389
OrthoDBiVOG09000028

Family and domain databases

HAMAPiMF_04065 INFV_RDRP, 1 hit
InterProiView protein in InterPro
IPR007099 RNA-dir_pol_NSvirus
IPR001407 RNA_pol_PB1_influenza
PfamiView protein in Pfam
PF00602 Flu_PB1, 1 hit
PIRSFiPIRSF000827 RdRPol_OMV, 1 hit
PROSITEiView protein in PROSITE
PS50525 RDRP_SSRNA_NEG_SEG, 1 hit

Sequencei

Sequence statusi: Complete.

P03431-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS
60 70 80 90 100
EKGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP
110 120 130 140 150
GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF
160 170 180 190 200
RSNGLTANES GRLIDFLKDV MESMKKEEMG ITTHFQRKRR VRDNMTKKMI
210 220 230 240 250
TQRTIGKKKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG
260 270 280 290 300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTELSF
310 320 330 340 350
TITGDNTKWN ENQNPRMFLA MITYMTRNQP EWFRNVLSIA PIMFSNKMAR
360 370 380 390 400
LGKGYMFESK SMKLRTQIPA EMLASIDLKY FNDSTRKKIE KIRPLLIEGT
410 420 430 440 450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWWDG LQSSDDFALI
460 470 480 490 500
VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG
510 520 530 540 550
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL
560 570 580 590 600
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLYN
610 620 630 640 650
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESM NNAVMMPAHG
660 670 680 690 700
PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GVLEDEQMYQ RCCNLFEKFF
710 720 730 740 750
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI

EELRRQK
Length:757
Mass (Da):86,576
Last modified:October 2, 2007 - v2
Checksum:iFB23E4976E06C18F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53G → A in AAA43581 (PubMed:6281731).Curated1
Sequence conflicti175K → N in ABO21706 (Ref. 4) Curated1
Sequence conflicti175K → N in ABD77683 (PubMed:15163504).Curated1
Sequence conflicti205I → M in ABO21706 (Ref. 4) Curated1
Sequence conflicti205I → M in ABD77683 (PubMed:15163504).Curated1
Sequence conflicti208K → R in AAA43581 (PubMed:6281731).Curated1
Sequence conflicti300F → L in AAA43581 (PubMed:6281731).Curated1
Sequence conflicti394P → S in ABO21706 (Ref. 4) Curated1
Sequence conflicti394P → S in ABD77683 (PubMed:15163504).Curated1
Sequence conflicti473L → H in AAA43581 (PubMed:6281731).Curated1
Sequence conflicti517I → S in AAA43581 (PubMed:6281731).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02151 Genomic RNA Translation: AAA43581.1
AF389116 Genomic RNA Translation: AAM75156.1
CY009450 Genomic RNA Translation: ABD77683.1
EF467819 Genomic RNA Translation: ABO21706.1
PIRiA93418 P1IV34
RefSeqiNP_040985.1, NC_002021.1

Genome annotation databases

GeneIDi956534
KEGGivg:956534

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02151 Genomic RNA Translation: AAA43581.1
AF389116 Genomic RNA Translation: AAM75156.1
CY009450 Genomic RNA Translation: ABD77683.1
EF467819 Genomic RNA Translation: ABO21706.1
PIRiA93418 P1IV34
RefSeqiNP_040985.1, NC_002021.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZNLX-ray2.30B1-81[»]
2ZTTX-ray2.10A/C679-757[»]
3A1GX-ray1.70A/C678-757[»]
ProteinModelPortaliP03431
SMRiP03431
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-56974N
IntActiP03431, 141 interactors
MINTiP03431

Proteomic databases

PRIDEiP03431

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi956534
KEGGivg:956534

Phylogenomic databases

KOiK19389
OrthoDBiVOG09000028

Enzyme and pathway databases

ReactomeiR-HSA-168255 Influenza Life Cycle
R-HSA-168271 Transport of Ribonucleoproteins into the Host Nucleus
R-HSA-168275 Entry of Influenza Virion into Host Cell via Endocytosis
R-HSA-168288 Fusion of the Influenza Virion to the Host Cell Endosome
R-HSA-168298 Release
R-HSA-168302 Budding
R-HSA-168303 Packaging of Eight RNA Segments
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-168330 Viral RNP Complexes in the Host Cell Nucleus
R-HSA-168333 NEP/NS2 Interacts with the Cellular Export Machinery
R-HSA-168336 Uncoating of the Influenza Virion
R-HSA-192814 vRNA Synthesis
R-HSA-192823 Viral mRNA Translation
R-HSA-192869 cRNA Synthesis
R-HSA-192905 vRNP Assembly

Miscellaneous databases

EvolutionaryTraceiP03431
PROiPR:P03431

Family and domain databases

HAMAPiMF_04065 INFV_RDRP, 1 hit
InterProiView protein in InterPro
IPR007099 RNA-dir_pol_NSvirus
IPR001407 RNA_pol_PB1_influenza
PfamiView protein in Pfam
PF00602 Flu_PB1, 1 hit
PIRSFiPIRSF000827 RdRPol_OMV, 1 hit
PROSITEiView protein in PROSITE
PS50525 RDRP_SSRNA_NEG_SEG, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRDRP_I34A1
AccessioniPrimary (citable) accession number: P03431
Secondary accession number(s): A4GXH1, Q20N30, Q8JUU7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 2, 2007
Last modified: May 23, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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