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Protein

Polymerase basic protein 2

Gene

PB2

Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with and inhibition of the host mitochondrial antiviral signaling protein MAVS.UniRule annotation6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei627Mammalian adaptationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing, Viral immunoevasion, Viral transcription

Enzyme and pathway databases

ReactomeiR-HSA-168255 Influenza Life Cycle
R-HSA-168271 Transport of Ribonucleoproteins into the Host Nucleus
R-HSA-168275 Entry of Influenza Virion into Host Cell via Endocytosis
R-HSA-168288 Fusion of the Influenza Virion to the Host Cell Endosome
R-HSA-168298 Release
R-HSA-168302 Budding
R-HSA-168303 Packaging of Eight RNA Segments
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-168330 Viral RNP Complexes in the Host Cell Nucleus
R-HSA-168333 NEP/NS2 Interacts with the Cellular Export Machinery
R-HSA-168336 Uncoating of the Influenza Virion
R-HSA-192814 vRNA Synthesis
R-HSA-192823 Viral mRNA Translation
R-HSA-192869 cRNA Synthesis
R-HSA-192905 vRNP Assembly

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase basic protein 2UniRule annotation
Alternative name(s):
RNA-directed RNA polymerase subunit P3UniRule annotation
Gene namesi
Name:PB2UniRule annotation
OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Taxonomic identifieri211044 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeAlphainfluenzavirus
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000116373 Componenti: Genome
  • UP000170967 Componenti: Genome
  • UP000009255 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host mitochondrion, Host nucleus, Virion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3317339

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000788341 – 759Polymerase basic protein 2Add BLAST759

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-terminus) with host MAVS (via N-terminus); this interaction inhibits host innate immune response.UniRule annotation4 Publications

Binary interactionsi

Protein-protein interaction databases

DIPiDIP-43997N
IntActiP03428, 172 interactors
MINTiP03428

Chemistry databases

BindingDBiP03428

Structurei

Secondary structure

1759
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP03428
SMRiP03428
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03428

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi736 – 739Nuclear localization signalUniRule annotation4

Sequence similaritiesi

Belongs to the influenza viruses PB2 family.UniRule annotationCurated

Phylogenomic databases

KOiK19388
OrthoDBiVOG0900002O

Family and domain databases

Gene3Di3.30.30.90, 1 hit
HAMAPiMF_04062 INV_PB2, 1 hit
InterProiView protein in InterPro
IPR001591 INV_PB2
IPR037258 PDB2_C
PfamiView protein in Pfam
PF00604 Flu_PB2, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001667 RNA_pol_PB2_orthomyxovir, 1 hit
SUPFAMiSSF160453 SSF160453, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform Polymerase basic protein 2 (identifier: P03428-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM
60 70 80 90 100
MAMKYPITAD KRITEMIPER NEQGQTLWSK MNDAGSDRVM VSPLAVTWWN
110 120 130 140 150
RNGPITNTVH YPKIYKTYFE RVERLKHGTF GPVHFRNQVK IRRRVDINPG
160 170 180 190 200
HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQDCKISP
210 220 230 240 250
LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV
260 270 280 290 300
RNDDVDQSLI IAARNIVRRA AVSADPLASL LEMCHSTQIG GIRMVDILRQ
310 320 330 340 350
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKR EEEVLTGNLQ
360 370 380 390 400
TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV
410 420 430 440 450
AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG
460 470 480 490 500
VEPIDNVMGM IGILPDMTPS IEMSMRGVRI SKMGVDEYSS TERVVVSIDR
510 520 530 540 550
FLRIRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN GPESVLVNTY
560 570 580 590 600
QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAIRG QYSGFVRTLF
610 620 630 640 650
QQMRDVLGTF DTAQIIKLLP FAAAPPKQSR MQFSSFTVNV RGSGMRILVR
660 670 680 690 700
GNSPVFNYNK ATKRLTVLGK DAGTLTEDPD EGTAGVESAV LRGFLILGKE
710 720 730 740 750
DKRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA

TKRIRMAIN
Length:759
Mass (Da):86,095
Last modified:March 6, 2007 - v2
Checksum:i10D2D1608AE536D2
GO
Isoform PB2-S1By similarity (identifier: P0DOG6-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P0DOG6.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:510
Mass (Da):58,096
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105I → M in ABD77685 (PubMed:7060132).Curated1
Sequence conflicti251R → K in ABD77685 (PubMed:7060132).Curated1
Sequence conflicti299R → K in ABD77685 (PubMed:7060132).Curated1
Sequence conflicti309D → G in ABD77685 (PubMed:7060132).Curated1
Sequence conflicti504I → V in ABD77685 (PubMed:7060132).Curated1
Sequence conflicti701D → N in ABO21705 (PubMed:11779399).Curated1
Sequence conflicti702K → R in ABD77685 (PubMed:7060132).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00603 Unassigned RNA Translation: CAA23855.1
AF389115 Genomic RNA Translation: AAM75155.1
EF467818 Genomic RNA Translation: ABO21705.1
CY009451 Genomic RNA Translation: ABD77685.1
RefSeqiNP_040987.1, NC_002023.1

Genome annotation databases

GeneIDi956536
KEGGivg:956536

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00603 Unassigned RNA Translation: CAA23855.1
AF389115 Genomic RNA Translation: AAM75155.1
EF467818 Genomic RNA Translation: ABO21705.1
CY009451 Genomic RNA Translation: ABD77685.1
RefSeqiNP_040987.1, NC_002023.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZTTX-ray2.10B/D1-37[»]
3A1GX-ray1.70B/D1-37[»]
3CW4X-ray2.70A535-759[»]
3WI0X-ray2.00A318-484[»]
3WI1X-ray1.93A318-484[»]
4ENFX-ray1.32A318-483[»]
4J2RX-ray2.42A/B318-484[»]
4U6OX-ray1.30A/B318-483[»]
ProteinModelPortaliP03428
SMRiP03428
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-43997N
IntActiP03428, 172 interactors
MINTiP03428

Chemistry databases

BindingDBiP03428
ChEMBLiCHEMBL3317339

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi956536
KEGGivg:956536

Phylogenomic databases

KOiK19388
OrthoDBiVOG0900002O

Enzyme and pathway databases

ReactomeiR-HSA-168255 Influenza Life Cycle
R-HSA-168271 Transport of Ribonucleoproteins into the Host Nucleus
R-HSA-168275 Entry of Influenza Virion into Host Cell via Endocytosis
R-HSA-168288 Fusion of the Influenza Virion to the Host Cell Endosome
R-HSA-168298 Release
R-HSA-168302 Budding
R-HSA-168303 Packaging of Eight RNA Segments
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-168330 Viral RNP Complexes in the Host Cell Nucleus
R-HSA-168333 NEP/NS2 Interacts with the Cellular Export Machinery
R-HSA-168336 Uncoating of the Influenza Virion
R-HSA-192814 vRNA Synthesis
R-HSA-192823 Viral mRNA Translation
R-HSA-192869 cRNA Synthesis
R-HSA-192905 vRNP Assembly

Miscellaneous databases

EvolutionaryTraceiP03428
PROiPR:P03428

Family and domain databases

Gene3Di3.30.30.90, 1 hit
HAMAPiMF_04062 INV_PB2, 1 hit
InterProiView protein in InterPro
IPR001591 INV_PB2
IPR037258 PDB2_C
PfamiView protein in Pfam
PF00604 Flu_PB2, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001667 RNA_pol_PB2_orthomyxovir, 1 hit
SUPFAMiSSF160453 SSF160453, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPB2_I34A1
AccessioniPrimary (citable) accession number: P03428
Secondary accession number(s): A4GXH0, Q20N28, Q8JUU8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 6, 2007
Last modified: October 10, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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