UniProtKB - P03417 (NCAP_CVMJH)
Protein
Nucleoprotein
Gene
N
Organism
Murine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus)
Status
Functioni
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.UniRule annotation1 Publication
GO - Molecular functioni
- RNA binding Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding, Viral nucleoprotein |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: NucleoproteinUniRule annotationAlternative name(s): Nucleocapsid proteinUniRule annotation Short name: NCUniRule annotation Short name: Protein NUniRule annotation |
Gene namesi | Name:NUniRule annotation ORF Names:7a |
Organismi | Murine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus) |
Taxonomic identifieri | 11144 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Embecovirus › |
Virus hosti | Mus musculus (Mouse) [TaxID: 10090] |
Proteomesi |
|
Subcellular locationi
- Virion UniRule annotation
- Host endoplasmic reticulum-Golgi intermediate compartment UniRule annotation
- Host Golgi apparatus UniRule annotation Note: Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding.UniRule annotation
GO - Cellular componenti
- host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
- host cell Golgi apparatus Source: UniProtKB-SubCell
- viral nucleocapsid Source: UniProtKB-UniRule
Keywords - Cellular componenti
Host Golgi apparatus, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106009 | 1 – 455 | NucleoproteinAdd BLAST | 455 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 170 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 177 | Phosphothreonine; by hostUniRule annotation | 1 | |
Modified residuei | 194 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 390 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 425 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 429 | Phosphothreonine; by hostUniRule annotation | 1 |
Post-translational modificationi
ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.UniRule annotation
Phosphorylated on serine and threonine residues (By similarity). Phosphorylated by host GSK3A and GSK3B. Phosphorylation allows recruitment of host RNA helicase DDX1 which facilitates template readthrough and enables longer subgenomic mRNA synthesis.UniRule annotation1 Publication
Keywords - PTMi
ADP-ribosylation, PhosphoproteinInteractioni
Subunit structurei
Homooligomer. Both monomeric and oligomeric forms interact with RNA.
Interacts with protein M.
Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection (By similarity).
Interacts (when phosphorylated) with host DDX1; this interaction is essential to produce a full set of subgenomic and genomic RNAs (PubMed:25299332).
UniRule annotation1 PublicationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 64 – 193 | CoV N NTDPROSITE-ProRule annotationAdd BLAST | 130 | |
Domaini | 260 – 383 | CoV N CTDPROSITE-ProRule annotationAdd BLAST | 124 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 56 – 197 | RNA-bindingUniRule annotationAdd BLAST | 142 | |
Regioni | 267 – 384 | DimerizationUniRule annotationAdd BLAST | 118 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 194 – 220 | Ser-richAdd BLAST | 27 |
Sequence similaritiesi
Belongs to the betacoronavirus nucleocapsid protein family.UniRule annotation
Family and domain databases
HAMAPi | MF_04096, BETA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR043505, NCAP_bCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
i Sequence
Sequence statusi: Complete.
P03417-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFVPGQENA GSRSSSGNRA GNGILKKTTW ADQTERGLNN QNRGRKNQPK
60 70 80 90 100
QTATTQPNSG SVVPHYSWFS GITQFQKGKE FQFAQGQGVP IANGIPASQQ
110 120 130 140 150
KGYWYRHNRR SFKTPDGQQK QLLPRWYFYY LGTGPYAGAE YGDDIEGVVW
160 170 180 190 200
VASQQAETRT SADIVERDPS SHEAIPTRFA PGTVLPQGFY VEGSGRSAPA
210 220 230 240 250
SRSGSRPQSR GPNNRARSSS NQRQPASTVK PDMAEEIAAL VLAKLGKDAG
260 270 280 290 300
QPKQVTKQSA KEVRQKILNK PRQKRTPNKQ CPVQQCFGKR GPNQNFGGPE
310 320 330 340 350
MLKLGTSDPQ FPILAELAPT AGAFFFGSKL ELVKKNSGGA DGPTKDVYEL
360 370 380 390 400
QYSGAVRFDS TLPGFETIMK VLNENLNAYQ NQDGGADVVS PKPQRKRGTK
410 420 430 440 450
QKAQKDEVDN VSVAKPKSSV QRNVSRELTP EDRSLLAQIL DDGVVPDGLE
DDSNV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00990 mRNA Translation: CAA25497.1 |
PIRi | A04024, VHIHMJ |
RefSeqi | YP_209238.1, AC_000192.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00990 mRNA Translation: CAA25497.1 |
PIRi | A04024, VHIHMJ |
RefSeqi | YP_209238.1, AC_000192.1 |
3D structure databases
SMRi | P03417 |
ModBasei | Search... |
Family and domain databases
HAMAPi | MF_04096, BETA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR043505, NCAP_bCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCAP_CVMJH | |
Accessioni | P03417Primary (citable) accession number: P03417 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 10, 2021 | |
This is version 100 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |