UniProtKB - P03345 (GAG_HTL1A)
Gag polyprotein
gag
Functioni
The matrix domain targets Gag, Gag-Pro and Gag-Pro-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus.
1 PublicationMatrix protein.
1 PublicationForms the spherical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
CuratedBinds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.
1 PublicationMiscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 355 – 372 | CCHC-type 1PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 378 – 395 | CCHC-type 2PROSITE-ProRule annotationAdd BLAST | 18 |
GO - Molecular functioni
- nucleic acid binding Source: InterPro
- structural molecule activity Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- viral process Source: InterPro
Keywordsi
Molecular function | Viral nucleoprotein |
Biological process | Host-virus interaction |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Gag polyproteinAlternative name(s): Pr53Gag Cleaved into the following 3 chains: |
Gene namesi | Name:gag |
Organismi | Human T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) (HTLV-1) |
Taxonomic identifieri | 11926 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Pararnavirae › Artverviricota › Revtraviricetes › Ortervirales › Retroviridae › Orthoretrovirinae › Deltaretrovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion 1 Publication
- Virion Curated
Keywords - Cellular componenti
Capsid protein, VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | G → A: Complete loss of myristoylation the polyprotein. The concomitent loss of binding to the host cell membrane impairs the formation of viral particles. 1 Publication | 1 | |
Mutagenesisi | 61 | C → S: Complete loss of homodimerization of matrix protein p19. 1 Publication | 1 | |
Mutagenesisi | 105 | S → A: 70% loss of viral particle release. 1 Publication | 1 | |
Mutagenesisi | 118 – 120 | PPP → AAA: Complete loss of interaction with human NEDD4. 1 Publication | 3 | |
Mutagenesisi | 124 – 127 | PTAP → ATAA: Complete loss of interaction with human TSG101. 1 Publication | 4 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed; by hostSequence analysis | |||
ChainiPRO_0000259771 | 2 – 429 | Gag polyproteinAdd BLAST | 428 | |
ChainiPRO_0000038811 | 2 – 130 | Matrix protein p19Add BLAST | 129 | |
ChainiPRO_0000038812 | 131 – 344 | Capsid protein p24Add BLAST | 214 | |
ChainiPRO_0000038813 | 345 – 429 | Nucleocapsid protein p15-gagAdd BLAST | 85 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine; by hostSequence analysis2 Publications | 1 | |
Disulfide bondi | 61 | Interchain1 Publication | ||
Modified residuei | 105 | Phosphoserine; by host MAPK11 Publication | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 130 – 131 | Cleavage; by viral protease1 Publication | 2 | |
Sitei | 344 – 345 | Cleavage; by viral protease1 Publication | 2 |
Keywords - PTMi
Disulfide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugationPTM databases
iPTMneti | P03345 |
Interactioni
Subunit structurei
Protein-protein interaction databases
ELMi | P03345 |
Structurei
Secondary structure
3D structure databases
BMRBi | P03345 |
SMRi | P03345 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P03345 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 93 – 144 | DisorderedSequence analysisAdd BLAST | 52 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 118 – 121 | PPXY motif1 Publication | 4 | |
Motifi | 124 – 127 | PTAP/PSAP motif1 Publication | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 95 – 126 | Pro residuesSequence analysisAdd BLAST | 32 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 355 – 372 | CCHC-type 1PROSITE-ProRule annotationAdd BLAST | 18 | |
Zinc fingeri | 378 – 395 | CCHC-type 2PROSITE-ProRule annotationAdd BLAST | 18 |
Keywords - Domaini
Repeat, Zinc-fingerFamily and domain databases
Gene3Di | 1.10.1200.30, 1 hit 1.10.375.10, 1 hit |
InterProi | View protein in InterPro IPR003139, D_retro_matrix IPR045345, Gag_p24_C IPR000721, Gag_p24_N IPR008916, Retrov_capsid_C IPR008919, Retrov_capsid_N IPR010999, Retrovr_matrix IPR001878, Znf_CCHC IPR036875, Znf_CCHC_sf |
Pfami | View protein in Pfam PF02228, Gag_p19, 1 hit PF00607, Gag_p24, 1 hit PF19317, Gag_p24_C, 1 hit PF00098, zf-CCHC, 1 hit |
SMARTi | View protein in SMART SM00343, ZnF_C2HC, 2 hits |
SUPFAMi | SSF47836, SSF47836, 1 hit SSF47943, SSF47943, 1 hit SSF57756, SSF57756, 1 hit |
PROSITEi | View protein in PROSITE PS50158, ZF_CCHC, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsiribosomal frameshifting. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGQIFSRSAS PIPRPPRGLA AHHWLNFLQA AYRLEPGPSS YDFHQLKKFL
60 70 80 90 100
KIALETPARI CPINYSLLAS LLPKGYPGRV NEILHILIQT QAQIPSRPAP
110 120 130 140 150
PPPSSPTHDP PDSDPQIPPP YVEPTAPQVL PVMHPHGAPP NHRPWQMKDL
160 170 180 190 200
QAIKQEVSQA APGSPQFMQT IRLAVQQFDP TAKDLQDLLQ YLCSSLVASL
210 220 230 240 250
HHQQLDSLIS EAETRGITGY NPLAGPLRVQ ANNPQQQGLR REYQQLWLAA
260 270 280 290 300
FAALPGSAKD PSWASILQGL EEPYHAFVER LNIALDNGLP EGTPKDPILR
310 320 330 340 350
SLAYSNANKE CQKLLQARGH TNSPLGDMLR ACQTWTPKDK TKVLVVQPKK
360 370 380 390 400
PPPNQPCFRC GKAGHWSRDC TQPRPPPGPC PLCQDPTHWK RDCPRLKPTI
410 420
PEPEPEEDAL LLDLPADIPH PKNSIGGEV
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 416 | A → T AA sequence (PubMed:6313426).Curated | 1 | |
Sequence conflicti | 424 | S → F in AAA96672 (PubMed:6304725).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02029 Genomic DNA Translation: AAA96672.1 M13810 Genomic RNA Translation: AAA46205.1 |
PIRi | B93954, FOLJGH |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02029 Genomic DNA Translation: AAA96672.1 M13810 Genomic RNA Translation: AAA46205.1 |
PIRi | B93954, FOLJGH |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QRJ | NMR | - | A | 146-344 | [»] | |
BMRBi | P03345 | |||||
SMRi | P03345 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
ELMi | P03345 |
PTM databases
iPTMneti | P03345 |
Miscellaneous databases
EvolutionaryTracei | P03345 |
Family and domain databases
Gene3Di | 1.10.1200.30, 1 hit 1.10.375.10, 1 hit |
InterProi | View protein in InterPro IPR003139, D_retro_matrix IPR045345, Gag_p24_C IPR000721, Gag_p24_N IPR008916, Retrov_capsid_C IPR008919, Retrov_capsid_N IPR010999, Retrovr_matrix IPR001878, Znf_CCHC IPR036875, Znf_CCHC_sf |
Pfami | View protein in Pfam PF02228, Gag_p19, 1 hit PF00607, Gag_p24, 1 hit PF19317, Gag_p24_C, 1 hit PF00098, zf-CCHC, 1 hit |
SMARTi | View protein in SMART SM00343, ZnF_C2HC, 2 hits |
SUPFAMi | SSF47836, SSF47836, 1 hit SSF47943, SSF47943, 1 hit SSF57756, SSF57756, 1 hit |
PROSITEi | View protein in PROSITE PS50158, ZF_CCHC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GAG_HTL1A | |
Accessioni | P03345Primary (citable) accession number: P03345 Secondary accession number(s): Q85589 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 138 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references