UniProtKB - P03345 (GAG_HTL1A)

BLAST

>sp|P03345|GAG_HTL1A Gag polyprotein OS=Human T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) OX=11926 GN=gag PE=1 SV=3
MGQIFSRSASPIPRPPRGLAAHHWLNFLQAAYRLEPGPSSYDFHQLKKFLKIALETPARI
CPINYSLLASLLPKGYPGRVNEILHILIQTQAQIPSRPAPPPPSSPTHDPPDSDPQIPPP
YVEPTAPQVLPVMHPHGAPPNHRPWQMKDLQAIKQEVSQAAPGSPQFMQTIRLAVQQFDP
TAKDLQDLLQYLCSSLVASLHHQQLDSLISEAETRGITGYNPLAGPLRVQANNPQQQGLR
REYQQLWLAAFAALPGSAKDPSWASILQGLEEPYHAFVERLNIALDNGLPEGTPKDPILR
SLAYSNANKECQKLLQARGHTNSPLGDMLRACQTWTPKDKTKVLVVQPKKPPPNQPCFRC
GKAGHWSRDCTQPRPPPGPCPLCQDPTHWKRDCPRLKPTIPEPEPEEDALLLDLPADIPH
PKNSIGGEV

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History
Entry version 125 (20 Jun 2018)
Sequence version 3 (23 Jan 2007)
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Protein

Gag polyprotein

Gene

gag

Organism

Human T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) (HTLV-1)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Gag polyprotein: The matrix domain targets Gag, Gag-Pro and Gag-Pro-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus.1 Publication

Matrix protein p19: Matrix protein.1 Publication

Capsid protein p24: Forms the spherical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.Curated

Nucleocapsid protein p15-gag: Binds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.1 Publication

Miscellaneous

HTLV-1 lineages are divided in four clades, A (Cosmopolitan), B (Central African group), C (Melanesian group) and D (New Central African group).Curated

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	355 – 372	CCHC-type 1PROSITE-ProRule annotationAdd BLAST		18
Zinc fingerⁱ	378 – 395	CCHC-type 2PROSITE-ProRule annotationAdd BLAST		18

GO - Molecular functionⁱ

nucleic acid binding Source: InterPro
structural molecule activity Source: InterPro
zinc ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

viral process Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Viral nucleoprotein
Biological process	Host-virus interaction
Ligand	Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Gag polyprotein Alternative name(s): Pr53Gag Cleaved into the following 3 chains: Matrix protein p19 Short name: MA Capsid protein p24 Short name: CA Nucleocapsid protein p15-gag Short name: NC-gag
Gene namesⁱ	Name:gag
Organismⁱ	Human T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) (HTLV-1)
Taxonomic identifierⁱ	11926 [NCBI]
Taxonomic lineageⁱ	Viruses › Ortervirales › Retroviridae › Orthoretrovirinae › Deltaretrovirus › Primate T-lymphotropic virus 1 › Human T-cell leukemia virus type I › HTLV-1 subtype A
Virus hostⁱ	Homo sapiens (Human) [TaxID: 9606]
Proteomesⁱ	UP000007683 Componentⁱ: Genome

Subcellular locationⁱ

Matrix protein p19 :

Virion
1 Publication
Manual assertion based on experiment inⁱ
- Ref.11
  "In vivo homodimerisation of HTLV-1 Gag and MA gives clues to the retroviral capsid and TM envelope protein arrangement."
  Rayne F., Kajava A.V., Lalanne J., Mamoun R.Z.
  J. Mol. Biol. 343:903-916(2004) [PubMed] [Europe PMC] [Abstract]
  Cited for: DISULFIDE BOND, SUBUNIT (GAG POLYPROTEIN), SUBUNIT (MATRIX PROTEIN P19), MUTAGENESIS OF CYS-61, SUBCELLULAR LOCATION (MATRIX PROTEIN P19), FUNCTION (GAG POLYPROTEIN), FUNCTION (MATRIX PROTEIN P19).

Capsid protein p24 :

Virion Curated

Nucleocapsid protein p15-gag :

Virion Curated

GO - Cellular componentⁱ

viral nucleocapsid Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Capsid protein, Virion

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	2	G → A: Complete loss of myristoylation the polyprotein. The concomitent loss of binding to the host cell membrane impairs the formation of viral particles. 1 Publication	1
Mutagenesisⁱ	61	C → S: Complete loss of homodimerization of matrix protein p19. 1 Publication	1
Mutagenesisⁱ	105	S → A: 70% loss of viral particle release. 1 Publication	1
Mutagenesisⁱ	118 – 120	PPP → AAA: Complete loss of interaction with human NEDD4. 1 Publication	3
Mutagenesisⁱ	124 – 127	PTAP → ATAA: Complete loss of interaction with human TSG101. 1 Publication	4

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Initiator methionineⁱ		Removed; by hostSequence analysis
ChainⁱPRO_0000259771	2 – 429	Gag polyproteinAdd BLAST	428
ChainⁱPRO_0000038811	2 – 130	Matrix protein p19Add BLAST	129
ChainⁱPRO_0000038812	131 – 344	Capsid protein p24Add BLAST	214
ChainⁱPRO_0000038813	345 – 429	Nucleocapsid protein p15-gagAdd BLAST	85

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Lipidationⁱ	2	N-myristoyl glycine; by hostSequence analysis2 Publications	1
Disulfide bondⁱ	61	Interchain1 Publication
Modified residueⁱ	105	Phosphoserine; by host MAPK11 Publication	1

Post-translational modificationⁱ

Gag polyprotein: Specific enzymatic cleavages by the viral protease yield mature proteins. The polyprotein is cleaved during and after budding, this process is termed maturation.2 Publications

Matrix protein p19: Phosphorylation of the matrix protein p19 by MAPK1 seems to play a role in budding.1 Publication

Gag polyprotein: Ubiquitinated by host NEDD4.1 Publication

Gag polyprotein: Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.2 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	130 – 131	Cleavage; by viral protease1 Publication		2
Siteⁱ	344 – 345	Cleavage; by viral protease1 Publication		2

Keywords - PTMⁱ

Disulfide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

PTM databases

iPTMnetⁱ	P03345

iPTMnetⁱ

P03345

Interactionⁱ

Subunit structureⁱ

Gag polyprotein: Homodimer; the homodimers are part of the immature particles (PubMed:15476809). Gag polyprotein: Interacts with human TSG101 and NEDD4; these interactions are essential for budding and release of viral particles (PubMed:14581525, PubMed:15126635). Matrix protein p19: Homodimer; further assembles as homohexamers (PubMed:15476809).3 Publications

Protein-protein interaction databases

ELMⁱ	P03345

ELMⁱ

P03345

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	147 – 159	Combined sources	13
Helixⁱ	167 – 177	Combined sources	11
Helixⁱ	182 – 189	Combined sources	8
Turnⁱ	190 – 192	Combined sources	3
Helixⁱ	195 – 214	Combined sources	20
Turnⁱ	215 – 219	Combined sources	5
Turnⁱ	222 – 224	Combined sources	3
Helixⁱ	228 – 231	Combined sources	4
Helixⁱ	238 – 251	Combined sources	14
Helixⁱ	263 – 265	Combined sources	3
Helixⁱ	274 – 286	Combined sources	13
Helixⁱ	296 – 303	Combined sources	8
Turnⁱ	304 – 306	Combined sources	3
Helixⁱ	309 – 318	Combined sources	10
Beta strandⁱ	321 – 323	Combined sources	3
Helixⁱ	325 – 332	Combined sources	8

Show more details

3D structure databases

ProteinModelPortalⁱ	P03345
SMRⁱ	P03345
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P03345

EvolutionaryTraceⁱ

P03345

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	118 – 121	PPXY motif1 Publication		4
Motifⁱ	124 – 127	PTAP/PSAP motif1 Publication		4

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	95 – 144	Pro-richPROSITE-ProRule annotationAdd BLAST		50

Domainⁱ

Gag polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Matrix protein p19 contains two L domains: a PTAP/PSAP motif which interacts with the UEV domain of TSG101, and a PPXY motif which binds to the WW domains of the ubiquitin ligase NEDD4.2 Publications

Capsid protein p24: The capsid protein N-terminus seems to be involved in Gag-Gag interactions.1 Publication

Nucleocapsid protein p15-gag: The C-terminus is acidic.1 Publication

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	355 – 372	CCHC-type 1PROSITE-ProRule annotationAdd BLAST		18
Zinc fingerⁱ	378 – 395	CCHC-type 2PROSITE-ProRule annotationAdd BLAST		18

Keywords - Domainⁱ

Repeat, Zinc-finger

Phylogenomic databases

Database of Orthologous Groups More...OrthoDBⁱ	VOG09000057

OrthoDBⁱ

VOG09000057

Family and domain databases

Gene3Dⁱ	1.10.1200.30, 1 hit 1.10.185.10, 1 hit 1.10.375.10, 1 hit
InterProⁱ	View protein in InterPro IPR003139 D_retro_matrix IPR036989 D_retro_matrix_sf IPR000721 Gag_p24 IPR008916 Retrov_capsid_C IPR008919 Retrov_capsid_N IPR010999 Retrovr_matrix IPR001878 Znf_CCHC IPR036875 Znf_CCHC_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02228 Gag_p19, 1 hit PF00607 Gag_p24, 1 hit PF00098 zf-CCHC, 1 hit
SMARTⁱ	View protein in SMART SM00343 ZnF_C2HC, 2 hits
SUPFAMⁱ	SSF47836 SSF47836, 1 hit SSF47943 SSF47943, 1 hit SSF57756 SSF57756, 1 hit
PROSITEⁱ	View protein in PROSITE PS50158 ZF_CCHC, 1 hit

Sequences (3)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsⁱ produced by ribosomal frameshifting. Align Add to basket

Note: This strategy of translation probably allows the virus to modulate the quantity of each viral protein.Curated

Isoform Gag polyprotein (identifier: P03345-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQIFSRSAS PIPRPPRGLA AHHWLNFLQA AYRLEPGPSS YDFHQLKKFL 
        60         70         80         90        100
KIALETPARI CPINYSLLAS LLPKGYPGRV NEILHILIQT QAQIPSRPAP 
       110        120        130        140        150
PPPSSPTHDP PDSDPQIPPP YVEPTAPQVL PVMHPHGAPP NHRPWQMKDL 
       160        170        180        190        200
QAIKQEVSQA APGSPQFMQT IRLAVQQFDP TAKDLQDLLQ YLCSSLVASL 
       210        220        230        240        250
HHQQLDSLIS EAETRGITGY NPLAGPLRVQ ANNPQQQGLR REYQQLWLAA 
       260        270        280        290        300
FAALPGSAKD PSWASILQGL EEPYHAFVER LNIALDNGLP EGTPKDPILR 
       310        320        330        340        350
SLAYSNANKE CQKLLQARGH TNSPLGDMLR ACQTWTPKDK TKVLVVQPKK 
       360        370        380        390        400
PPPNQPCFRC GKAGHWSRDC TQPRPPPGPC PLCQDPTHWK RDCPRLKPTI 
       410        420 
PEPEPEEDAL LLDLPADIPH PKNSIGGEV

Note: Produced by conventional translation.1 Publication

Length:429

Mass (Da):47,496

Last modified:January 23, 2007 - v3

Checksum:ⁱ03FFD4E5A4500284

Isoform Gag-Pro polyprotein (identifier: P10274-1) [UniParc]FASTA Add to basket

The sequence of this isoform can be found in the external entry P10274.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by -1 ribosomal frameshifting at the gag-pro genes boundary.1 Publication

Length:651

Mass (Da):71,558

Isoform Gag-Pro-Pol polyprotein (identifier: P03362-1) [UniParc]FASTA Add to basket

The sequence of this isoform can be found in the external entry P03362.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by -1 ribosomal frameshiftings at the gag-pro and gag-pol genes boundaries.1 Publication

Length:1,462

Mass (Da):162,512

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	416	A → T AA sequence (PubMed:6313426).Curated		1
Sequence conflictⁱ	424	S → F in AAA96672 (PubMed:6304725).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J02029 Genomic DNA Translation: AAA96672.1 M13810 Genomic RNA Translation: AAA46205.1
PIRⁱ	B93954 FOLJGH

Keywords - Coding sequence diversityⁱ

Ribosomal frameshifting

Protein	Similar proteins		Species	Score	Length	Source
P03345	Gag protein (Fragment)		Human T-cell leukemia virus type I		326	UniRef100_P03345
Gag protein (Fragment)		Human T-cell leukemia virus type I		326

Protein	Similar proteins		Species	Score	Length	Source
P03345	Gag polyprotein	)	HTL1M		429	UniRef90_P03345
Gag polyprotein		HTL1L		429
Gag polyprotein		HTL1C		429
Gag protein (Fragment)		Human T-cell leukemia virus type I		326
Gag polyprotein		9STL1		433
+180

Protein	Similar proteins		Species	Score	Length	Source
P03345	Gag polyprotein	)	HTL1M		429	UniRef50_P03345
Gag polyprotein		HTL1L		429
Gag polyprotein		HTL1C		429
Gag polyprotein	)	HTLV2		433
Gag polyprotein		HTL3P		422
+214

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J02029 Genomic DNA Translation: AAA96672.1 M13810 Genomic RNA Translation: AAA46205.1
PIRⁱ	B93954 FOLJGH

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1QRJ	NMR	-	A	146-344	[»]
ProteinModelPortalⁱ	P03345
SMRⁱ	P03345
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

ELMⁱ	P03345

ELMⁱ

P03345

PTM databases

iPTMnetⁱ	P03345

iPTMnetⁱ

P03345

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Phylogenomic databases

OrthoDBⁱ	VOG09000057

OrthoDBⁱ

VOG09000057

Miscellaneous databases

EvolutionaryTraceⁱ	P03345

EvolutionaryTraceⁱ

P03345

Family and domain databases

Gene3Dⁱ	1.10.1200.30, 1 hit 1.10.185.10, 1 hit 1.10.375.10, 1 hit
InterProⁱ	View protein in InterPro IPR003139 D_retro_matrix IPR036989 D_retro_matrix_sf IPR000721 Gag_p24 IPR008916 Retrov_capsid_C IPR008919 Retrov_capsid_N IPR010999 Retrovr_matrix IPR001878 Znf_CCHC IPR036875 Znf_CCHC_sf
Pfamⁱ	View protein in Pfam PF02228 Gag_p19, 1 hit PF00607 Gag_p24, 1 hit PF00098 zf-CCHC, 1 hit
SMARTⁱ	View protein in SMART SM00343 ZnF_C2HC, 2 hits
SUPFAMⁱ	SSF47836 SSF47836, 1 hit SSF47943 SSF47943, 1 hit SSF57756 SSF57756, 1 hit
PROSITEⁱ	View protein in PROSITE PS50158 ZF_CCHC, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GAG_HTL1A
Accessionⁱ	P03345Primary (citable) accession number: P03345 Secondary accession number(s): Q85589
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	January 23, 2007
	Last modified:	June 20, 2018
	This is version 125 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB

UniParc

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UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P03345 (GAG_HTL1A)

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Gag polyprotein

gag

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Sites

Keywords - PTMi

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Compositional bias

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Protocols and materials databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ