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Protein

Gag polyprotein

Gene

gag

Organism
Friend spleen focus-forming virus (isolate 502) (FSFFV)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release (By similarity).By similarity
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).By similarity

Caution

This gag polyprotein does not encode any capsid and nucleoprotein.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 2 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Gene namesi
Name:gag
OrganismiFriend spleen focus-forming virus (isolate 502) (FSFFV)
Taxonomic identifieri355329 [NCBI]
Taxonomic lineageiVirusesOrterviralesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostiMus [TaxID: 10088]

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
ChainiPRO_00003907992 – 187Gag polyproteinAdd BLAST186
ChainiPRO_00000408412 – 129Matrix protein p15Sequence analysisAdd BLAST128
ChainiPRO_0000040842130 – 187RNA-binding phosphoprotein p12Sequence analysisAdd BLAST58

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostBy similarity1

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei129 – 130Cleavage; by viral proteaseBy similarity2

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Protein-protein interaction databases

ELMiP03331

Structurei

3D structure databases

ProteinModelPortaliP03331
SMRiP03331
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi109 – 112PTAP/PSAP motif4
Motifi128 – 132LYPX(n)L motif5

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 contains one L domain: a LYPX(n)L motif which interacts with PDCD6IP (By similarity).By similarity

Family and domain databases

Gene3Di1.10.150.180, 1 hit
InterProiView protein in InterPro
IPR000840 G_retro_matrix
IPR036946 G_retro_matrix_sf
IPR010999 Retrovr_matrix
PfamiView protein in Pfam
PF01140 Gag_MA, 1 hit
SUPFAMiSSF47836 SSF47836, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQTVTTPLS LTLEHWEDVQ RTASNQSVDV KKRRWVTFCS AEWPTFGVGW
60 70 80 90 100
PQDGTFNLDI ILQVKSKVFS PGPHGHPDQV PYIVTWEAIA YEPPPWVKPF
110 120 130 140 150
VSPKLSPSPT APILPSGPST QPPPRSALYP ALTPSIKPGP SPIMADLSLT
160 170 180
FSQKTLRRTE DRDRPPLTEM ATEKRPPPLL RFLPPLP
Length:187
Mass (Da):20,804
Last modified:January 23, 2007 - v3
Checksum:i7B0774B671E31185
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00021 Genomic RNA Translation: AAA46486.1
PIRiA03929 FOVW5S
RefSeqiNP_041217.1, NC_001500.1

Genome annotation databases

GeneIDi1491887
KEGGivg:1491887

Similar proteinsi

Entry informationi

Entry nameiGAG_FRSF5
AccessioniPrimary (citable) accession number: P03331
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 94 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

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